{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:19:35Z","timestamp":1792678775121,"version":"4.5.0"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2011,10,25]],"date-time":"2011-10-25T00:00:00Z","timestamp":1319500800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetologia"],"published-print":{"date-parts":[[2012,2]]},"DOI":"10.1007\/s00125-011-2344-9","type":"journal-article","created":{"date-parts":[[2011,10,24]],"date-time":"2011-10-24T18:49:48Z","timestamp":1319482188000},"page":"372-381","source":"Crossref","is-referenced-by-count":156,"title":["Immunohistochemical characterisation of cells co-producing insulin and glucagon in the developing human pancreas"],"prefix":"10.1007","volume":"55","author":[{"given":"M. J.","family":"Riedel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Asadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Ao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. L.","family":"Warnock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Kieffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,10,25]]},"reference":[{"key":"2344_CR1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1677\/joe.0.1810011","volume":"181","author":"K Piper","year":"2004","unstructured":"Piper K, Brickwood S, Turnpenny LW et al (2004) Beta cell differentiation during early human pancreas development. J Endocrinol 181:11\u201323","journal-title":"J Endocrinol"},{"key":"2344_CR2","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1101\/gad.269003","volume":"17","author":"P Collombat","year":"2003","unstructured":"Collombat P, Mansouri A, Hecksher-Sorensen J et al (2003) Opposing actions of Arx and Pax4 in endocrine pancreas development. Genes Dev 17:2591\u20132603","journal-title":"Genes Dev"},{"key":"2344_CR3","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1210\/en.2004-1576","volume":"146","author":"JF Habener","year":"2005","unstructured":"Habener JF, Kemp DM, Thomas MK (2005) Minireview: transcriptional regulation in pancreatic development. Endocrinology 146:1025\u20131034","journal-title":"Endocrinology"},{"key":"2344_CR4","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0012-1606(92)90121-V","volume":"153","author":"RR Krijger de","year":"1992","unstructured":"de Krijger RR, Aanstoot HJ, Kranenburg G, Reinhard M, Visser WJ, Bruining GJ (1992) The midgestational human fetal pancreas contains cells coexpressing islet hormones. Dev Biol 153:368\u2013375","journal-title":"Dev Biol"},{"key":"2344_CR5","first-page":"759","volume":"2","author":"LI Larsson","year":"1994","unstructured":"Larsson LI, Hougaard DM (1994) Coexpression of islet hormones and messenger RNAs in the human foetal pancreas. Endocrine 2:759\u2013765","journal-title":"Endocrine"},{"key":"2344_CR6","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.1242\/dev.127.11.2317","volume":"127","author":"PL Herrera","year":"2000","unstructured":"Herrera PL (2000) Adult insulin- and glucagon-producing cells differentiate from two independent cell lineages. Development 127:2317\u20132322","journal-title":"Development"},{"key":"2344_CR7","doi-asserted-by":"crossref","first-page":"12999","DOI":"10.1073\/pnas.91.26.12999","volume":"91","author":"PL Herrera","year":"1994","unstructured":"Herrera PL, Huarte J, Zufferey R et al (1994) Ablation of islet endocrine cells by targeted expression of hormone-promoter-driven toxigenes. Proc Natl Acad Sci USA 91:12999\u201313003","journal-title":"Proc Natl Acad Sci USA"},{"key":"2344_CR8","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0092-8674(88)90391-1","volume":"53","author":"S Alpert","year":"1988","unstructured":"Alpert S, Hanahan D, Teitelman G (1988) Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons. Cell 53:295\u2013308","journal-title":"Cell"},{"key":"2344_CR9","doi-asserted-by":"crossref","first-page":"G608","DOI":"10.1152\/ajpgi.00024.2010","volume":"298","author":"Y Fujita","year":"2010","unstructured":"Fujita Y, Asadi A, Yang GK, Kwok YN, Kieffer TJ (2010) Differential processing of pro-glucose-dependent insulinotropic polypeptide in gut. Am J Physiol Gastrointest Liver Physiol 298:G608\u2013G614","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"2344_CR10","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/BF01236270","volume":"17","author":"F Malaisse-Lagae","year":"1979","unstructured":"Malaisse-Lagae F, Stefan Y, Cox J, Perrelet A, Orci L (1979) Identification of a lobe in the adult human pancreas rich in pancreatic polypeptide. Diabetologia 17:361\u2013365","journal-title":"Diabetologia"},{"key":"2344_CR11","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s001090050118","volume":"75","author":"M Sander","year":"1997","unstructured":"Sander M, German MS (1997) The beta cell transcription factors and development of the pancreas. J Mol Med 75:327\u2013340","journal-title":"J Mol Med"},{"key":"2344_CR12","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1038\/387406a0","volume":"387","author":"L St-Onge","year":"1997","unstructured":"St-Onge L, Sosa-Pineda B, Chowdhury K, Mansouri A, Gruss P (1997) Pax6 is required for differentiation of glucagon-producing alpha-cells in mouse pancreas. Nature 387:406\u2013409","journal-title":"Nature"},{"key":"2344_CR13","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.ydbio.2003.12.008","volume":"268","author":"RS Heller","year":"2004","unstructured":"Heller RS, Stoffers DA, Liu A et al (2004) The role of Brn4\/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity. Dev Biol 268:123\u2013134","journal-title":"Dev Biol"},{"key":"2344_CR14","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1073\/pnas.0306233101","volume":"101","author":"TA Matsuoka","year":"2004","unstructured":"Matsuoka TA, Artner I, Henderson E, Means A, Sander M, Stein R (2004) The MafA transcription factor appears to be responsible for tissue-specific expression of insulin. Proc Natl Acad Sci USA 101:2930\u20132933","journal-title":"Proc Natl Acad Sci USA"},{"key":"2344_CR15","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1007\/s00125-006-0490-2","volume":"50","author":"H Wang","year":"2007","unstructured":"Wang H, Brun T, Kataoka K, Sharma AJ, Wollheim CB (2007) MAFA controls genes implicated in insulin biosynthesis and secretion. Diabetologia 50:348\u2013358","journal-title":"Diabetologia"},{"key":"2344_CR16","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1128\/MCB.25.12.4969-4976.2005","volume":"25","author":"C Zhang","year":"2005","unstructured":"Zhang C, Moriguchi T, Kajihara M et al (2005) MafA is a key regulator of glucose-stimulated insulin secretion. Mol Cell Biol 25:4969\u20134976","journal-title":"Mol Cell Biol"},{"key":"2344_CR17","doi-asserted-by":"crossref","first-page":"3853","DOI":"10.1073\/pnas.0700013104","volume":"104","author":"I Artner","year":"2007","unstructured":"Artner I, Blanchi B, Raum JC et al (2007) MafB is required for islet beta cell maturation. Proc Natl Acad Sci USA 104:3853\u20133858","journal-title":"Proc Natl Acad Sci USA"},{"key":"2344_CR18","doi-asserted-by":"crossref","first-page":"297","DOI":"10.2337\/diabetes.55.02.06.db05-0946","volume":"55","author":"I Artner","year":"2006","unstructured":"Artner I, Le Lay J, Hang Y et al (2006) MafB: an activator of the glucagon gene expressed in developing islet alpha- and beta-cells. Diabetes 55:297\u2013304","journal-title":"Diabetes"},{"key":"2344_CR19","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s00125-007-0880-0","volume":"51","author":"SA Sarkar","year":"2008","unstructured":"Sarkar SA, Kobberup S, Wong R et al (2008) Global gene expression profiling and histochemical analysis of the developing human fetal pancreas. Diabetologia 51:285\u2013297","journal-title":"Diabetologia"},{"key":"2344_CR20","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1038\/ni.1897","volume":"11","author":"H Kim","year":"2010","unstructured":"Kim H, Seed B (2010) The transcription factor MafB antagonizes antiviral responses by blocking recruitment of coactivators to the transcription factor IRF3. Nat Immunol 11:743\u2013750","journal-title":"Nat Immunol"},{"key":"2344_CR21","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1242\/dev.125.12.2213","volume":"125","author":"L Sussel","year":"1998","unstructured":"Sussel L, Kalamaras J, Hartigan-O\u2019Connor DJ et al (1998) Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 125:2213\u20132221","journal-title":"Development"},{"key":"2344_CR22","doi-asserted-by":"crossref","first-page":"12203","DOI":"10.1073\/pnas.91.25.12203","volume":"91","author":"A Rudnick","year":"1994","unstructured":"Rudnick A, Ling TY, Odagiri H, Rutter WJ, German MS (1994) Pancreatic beta cells express a diverse set of homeobox genes. Proc Natl Acad Sci USA 91:12203\u201312207","journal-title":"Proc Natl Acad Sci USA"},{"key":"2344_CR23","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s00125-008-1006-z","volume":"51","author":"BM Lyttle","year":"2008","unstructured":"Lyttle BM, Li J, Krishnamurthy M et al (2008) Transcription factor expression in the developing human fetal endocrine pancreas. Diabetologia 51:1169\u20131180","journal-title":"Diabetologia"},{"key":"2344_CR24","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.ydbio.2005.10.001","volume":"288","author":"JK Pedersen","year":"2005","unstructured":"Pedersen JK, Nelson SB, Jorgensen MC et al (2005) Endodermal expression of Nkx6 genes depends differentially on Pdx1. Dev Biol 288:487\u2013501","journal-title":"Dev Biol"},{"key":"2344_CR25","doi-asserted-by":"crossref","first-page":"5533","DOI":"10.1242\/dev.127.24.5533","volume":"127","author":"M Sander","year":"2000","unstructured":"Sander M, Sussel L, Conners J et al (2000) Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas. Development 127:5533\u20135540","journal-title":"Development"},{"key":"2344_CR26","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1172\/JCI200316537","volume":"111","author":"JD Johnson","year":"2003","unstructured":"Johnson JD, Ahmed NT, Luciani DS et al (2003) Increased islet apoptosis in Pdx1+\/\u2212 mice. J Clin Invest 111:1147\u20131160","journal-title":"J Clin Invest"},{"key":"2344_CR27","doi-asserted-by":"crossref","first-page":"12236","DOI":"10.1073\/pnas.192255099","volume":"99","author":"AM Holland","year":"2002","unstructured":"Holland AM, Hale MA, Kagami H, Hammer RE, MacDonald RJ (2002) Experimental control of pancreatic development and maintenance. Proc Natl Acad Sci USA 99:12236\u201312241","journal-title":"Proc Natl Acad Sci USA"},{"key":"2344_CR28","doi-asserted-by":"crossref","first-page":"2969","DOI":"10.1242\/dev.01870","volume":"132","author":"P Collombat","year":"2005","unstructured":"Collombat P, Hecksher-Sorensen J, Broccoli V et al (2005) The simultaneous loss of Arx and Pax4 genes promotes a somatostatin-producing cell fate specification at the expense of the alpha- and beta-cell lineages in the mouse endocrine pancreas. Development 132:2969\u20132980","journal-title":"Development"},{"key":"2344_CR29","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/0012-1606(72)90083-8","volume":"29","author":"RL Pictet","year":"1972","unstructured":"Pictet RL, Clark WR, Williams RH, Rutter WJ (1972) An ultrastructural analysis of the developing embryonic pancreas. Dev Biol 29:436\u2013467","journal-title":"Dev Biol"},{"key":"2344_CR30","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1369\/jhc.2009.953307","volume":"57","author":"J Jeon","year":"2009","unstructured":"Jeon J, Correa-Medina M, Ricordi C, Edlund H, Diez JA (2009) Endocrine cell clustering during human pancreas development. J Histochem Cytochem 57:811\u2013824","journal-title":"J Histochem Cytochem"},{"key":"2344_CR31","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/dvdy.20547","volume":"234","author":"M Castaing","year":"2005","unstructured":"Castaing M, Duvillie B, Quemeneur E, Basmaciogullari A, Scharfmann R (2005) Ex vivo analysis of acinar and endocrine cell development in the human embryonic pancreas. Dev Dyn 234:339\u2013345","journal-title":"Dev Dyn"},{"key":"2344_CR32","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1210\/en.138.4.1736","volume":"138","author":"L Scaglia","year":"1997","unstructured":"Scaglia L, Cahill CJ, Finegood DT, Bonner-Weir S (1997) Apoptosis participates in the remodeling of the endocrine pancreas in the neonatal rat. Endocrinology 138:1736\u20131741","journal-title":"Endocrinology"},{"key":"2344_CR33","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/0012-1606(92)90122-W","volume":"153","author":"A Lukinius","year":"1992","unstructured":"Lukinius A, Ericsson JL, Grimelius L, Korsgren O (1992) Ultrastructural studies of the ontogeny of fetal human and porcine endocrine pancreas, with special reference to colocalization of the four major islet hormones. Dev Biol 153:376\u2013385","journal-title":"Dev Biol"},{"key":"2344_CR34","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s004180050401","volume":"112","author":"J Bocian-Sobkowska","year":"1999","unstructured":"Bocian-Sobkowska J, Zabel M, Wozniak W, Surdyk-Zasada J (1999) Polyhormonal aspect of the endocrine cells of the human fetal pancreas. Histochem Cell Biol 112:147\u2013153","journal-title":"Histochem Cell Biol"},{"key":"2344_CR35","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1242\/dev.118.4.1031","volume":"118","author":"G Teitelman","year":"1993","unstructured":"Teitelman G, Alpert S, Polak JM, Martinez A, Hanahan D (1993) Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide. Development 118:1031\u20131039","journal-title":"Development"},{"key":"2344_CR36","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1038\/nbt1259","volume":"24","author":"KA D\u2019Amour","year":"2006","unstructured":"D\u2019Amour KA, Bang AG, Eliazer S et al (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24:1392\u20131401","journal-title":"Nat Biotechnol"},{"key":"2344_CR37","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1634\/stemcells.2006-0761","volume":"25","author":"J Jiang","year":"2007","unstructured":"Jiang J, Au M, Lu K et al (2007) Generation of insulin-producing islet-like clusters from human embryonic stem cells. Stem Cells 25:1940\u20131953","journal-title":"Stem Cells"},{"key":"2344_CR38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.2337\/db10-0573","volume":"60","author":"A Rezania","year":"2011","unstructured":"Rezania A, Riedel MJ, Wideman RD et al (2011) Production of functional glucagon-secreting alpha-cells from human embryonic stem cells. Diabetes 60:239\u2013247","journal-title":"Diabetes"},{"key":"2344_CR39","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1038\/nbt.1931","volume":"29","author":"OG Kelly","year":"2011","unstructured":"Kelly OG, Chan MY, Martinson LA et al (2011) Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol 29:750\u2013756","journal-title":"Nat Biotechnol"},{"key":"2344_CR40","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.cell.2009.05.035","volume":"138","author":"P Collombat","year":"2009","unstructured":"Collombat P, Xu X, Ravassard P et al (2009) The ectopic expression of Pax4 in the mouse pancreas converts progenitor cells into alpha and subsequently beta cells. Cell 138:449\u2013462","journal-title":"Cell"},{"key":"2344_CR41","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/nature08894","volume":"464","author":"F Thorel","year":"2010","unstructured":"Thorel F, Nepote V, Avril I et al (2010) Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss. Nature 464:1149\u20131154","journal-title":"Nature"},{"key":"2344_CR42","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1053\/j.gastro.2010.01.046","volume":"138","author":"J Lu","year":"2010","unstructured":"Lu J, Herrera PL, Carreira C et al (2010) Alpha-cell-specific Men1 ablation triggers the transdifferentiation of glucagon-expressing cells and insulinoma development. Gastroenterology 138:1954\u20131965","journal-title":"Gastroenterology"},{"key":"2344_CR43","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1002\/stem.482","volume":"28","author":"CH Chung","year":"2010","unstructured":"Chung CH, Hao E, Piran R, Keinan E, Levine F (2010) Pancreatic beta-cell neogenesis by direct conversion from mature alpha-cells. Stem Cells 28:1630\u20131638","journal-title":"Stem Cells"},{"key":"2344_CR44","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1007\/s001250100012","volume":"44","author":"M Castaing","year":"2001","unstructured":"Castaing M, Peault B, Basmaciogullari A, Casal I, Czernichow P, Scharfmann R (2001) Blood glucose normalization upon transplantation of human embryonic pancreas into beta-cell-deficient SCID mice. Diabetologia 44:2066\u20132076","journal-title":"Diabetologia"},{"key":"2344_CR45","doi-asserted-by":"crossref","first-page":"e3559","DOI":"10.1371\/journal.pone.0003559","volume":"3","author":"R Scharfmann","year":"2008","unstructured":"Scharfmann R, Xiao X, Heimberg H, Mallet J, Ravassard P (2008) Beta cells within single human islets originate from multiple progenitors. PLoS One 3:e3559","journal-title":"PLoS One"},{"key":"2344_CR46","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1038\/nprot.2008.118","volume":"3","author":"S Speier","year":"2008","unstructured":"Speier S, Nyqvist D, Kohler M, Caicedo A, Leibiger IB, Berggren PO (2008) Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye. Nat Protoc 3:1278\u20131286","journal-title":"Nat Protoc"},{"key":"2344_CR47","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1038\/nm1701","volume":"14","author":"S Speier","year":"2008","unstructured":"Speier S, Nyqvist D, Cabrera O et al (2008) Noninvasive in vivo imaging of pancreatic islet cell biology. Nat Med 14:574\u2013578","journal-title":"Nat Med"}],"container-title":["Diabetologia"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00125-011-2344-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00125-011-2344-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00125-011-2344-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,12]],"date-time":"2021-12-12T14:05:55Z","timestamp":1639317955000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00125-011-2344-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,25]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["2344"],"URL":"https:\/\/doi.org\/10.1007\/s00125-011-2344-9","relation":{},"ISSN":["0012-186X","1432-0428"],"issn-type":[{"value":"0012-186X","type":"print"},{"value":"1432-0428","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,25]]}}}