{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:14:01Z","timestamp":1786979641967,"version":"build-2736575974"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["2022FRBXHY"],"award-info":[{"award-number":["2022FRBXHY"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["2022TJWNLL"],"award-info":[{"award-number":["2022TJWNLL"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["CN00000041"],"award-info":[{"award-number":["CN00000041"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["P2022445ES"],"award-info":[{"award-number":["P2022445ES"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["P2022EB5B8"],"award-info":[{"award-number":["P2022EB5B8"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PNC 0000001 D3 4 Health"],"award-info":[{"award-number":["PNC 0000001 D3 4 Health"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["GR-2018-12365577"],"award-info":[{"award-number":["GR-2018-12365577"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["T4-AN-07"],"award-info":[{"award-number":["T4-AN-07"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Diabetologia"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1007\/s00125-026-06766-7","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:16:29Z","timestamp":1780758989000},"page":"2620-2633","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Plasma small RNA profiling reveals a three-miRNA signature associated with early beta cell dysfunction across glucose tolerance stages"],"prefix":"10.1007","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2054-4369","authenticated-orcid":false,"given":"Elena","family":"Aiello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7325-6128","authenticated-orcid":false,"given":"Giuseppina Emanuela","family":"Grieco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0139-4749","authenticated-orcid":false,"given":"Michela","family":"Brunetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1065-6030","authenticated-orcid":false,"given":"Giulia","family":"Gliozzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8803-5846","authenticated-orcid":false,"given":"Daniela","family":"Fignani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0001-9479","authenticated-orcid":false,"given":"Giuseppe","family":"Quero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7813-4426","authenticated-orcid":false,"given":"Sergio","family":"Alfieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4506-5193","authenticated-orcid":false,"given":"Gianfranco","family":"Di Giuseppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3621-6298","authenticated-orcid":false,"given":"Gea","family":"Ciccarelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6176-4379","authenticated-orcid":false,"given":"Angela","family":"Dardano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Parenti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1436-5591","authenticated-orcid":false,"given":"Andrea","family":"Mari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8251-1730","authenticated-orcid":false,"given":"Roberto","family":"Bizzotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6301-9161","authenticated-orcid":false,"given":"Giuseppe","family":"Daniele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7462-7792","authenticated-orcid":false,"given":"Andrea","family":"Giaccari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4947-7478","authenticated-orcid":false,"given":"Francesco","family":"Dotta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4374-8564","authenticated-orcid":false,"given":"Guido","family":"Sebastiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5407-9576","authenticated-orcid":false,"given":"Teresa","family":"Mezza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"6766_CR1","doi-asserted-by":"publisher","first-page":"15019","DOI":"10.1038\/nrdp.2015.19","volume":"1","author":"RA DeFronzo","year":"2015","unstructured":"DeFronzo RA, Ferrannini E, Groop L et al (2015) Type 2 diabetes mellitus. Nat Rev Dis Primers 1:15019. https:\/\/doi.org\/10.1038\/nrdp.2015.19","journal-title":"Nat Rev Dis Primers"},{"key":"6766_CR2","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1016\/j.metabol.2014.05.012","volume":"63","author":"E Ferrannini","year":"2014","unstructured":"Ferrannini E, Mari A (2014) \u03b2-Cell function in type 2 diabetes. Metab Clin Exp 63:1217\u20131227. https:\/\/doi.org\/10.1016\/j.metabol.2014.05.012","journal-title":"Metab Clin Exp"},{"key":"6766_CR3","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/S2213-8587(20)30022-X","volume":"8","author":"GC Weir","year":"2020","unstructured":"Weir GC, Gaglia J, Bonner-Weir S (2020) Inadequate \u03b2-cell mass is essential for the pathogenesis of type 2 diabetes. Lancet Diabetes Endocrinol 8:249\u2013256. https:\/\/doi.org\/10.1016\/S2213-8587(20)30022-X","journal-title":"Lancet Diabetes Endocrinol"},{"key":"6766_CR4","doi-asserted-by":"publisher","first-page":"e146788","DOI":"10.1172\/JCI146788","volume":"131","author":"T Mezza","year":"2021","unstructured":"Mezza T, Ferraro PM, Di Giuseppe G et al (2021) Pancreaticoduodenectomy model demonstrates a fundamental role of dysfunctional \u03b2 cells in predicting diabetes. J Clin Invest 131:e146788. https:\/\/doi.org\/10.1172\/JCI146788","journal-title":"J Clin Invest"},{"key":"6766_CR5","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.tcb.2011.12.001","volume":"22","author":"X Chen","year":"2012","unstructured":"Chen X, Liang H, Zhang J et al (2012) Secreted microRNAs: a new form of intercellular communication. Trends Cell Biol 22:125\u2013132. https:\/\/doi.org\/10.1016\/j.tcb.2011.12.001","journal-title":"Trends Cell Biol"},{"key":"6766_CR6","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/s0092-8674(04)00045-5","volume":"116","author":"DP Bartel","year":"2004","unstructured":"Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281\u2013297. https:\/\/doi.org\/10.1016\/s0092-8674(04)00045-5","journal-title":"Cell"},{"key":"6766_CR7","doi-asserted-by":"publisher","first-page":"7716","DOI":"10.3390\/ijms22147716","volume":"22","author":"C Formichi","year":"2021","unstructured":"Formichi C, Nigi L, Grieco GE et al (2021) Non-coding RNAs: novel players in insulin resistance and related diseases. Int J Mol Sci 22:7716. https:\/\/doi.org\/10.3390\/ijms22147716","journal-title":"Int J Mol Sci"},{"key":"6766_CR8","doi-asserted-by":"publisher","first-page":"3705","DOI":"10.3390\/ijms19123705","volume":"19","author":"L Nigi","year":"2018","unstructured":"Nigi L, Grieco GE, Ventriglia G et al (2018) MicroRNAs as regulators of insulin signaling: research updates and potential therapeutic perspectives in type 2 diabetes. Int J Mol Sci 19:3705. https:\/\/doi.org\/10.3390\/ijms19123705","journal-title":"Int J Mol Sci"},{"key":"6766_CR9","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1038\/s41420-022-01142-x","volume":"8","author":"GE Grieco","year":"2022","unstructured":"Grieco GE, Brusco N, Fignani D et al (2022) Reduced miR-184-3p expression protects pancreatic \u03b2-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation. Cell Death Discov 8:340. https:\/\/doi.org\/10.1038\/s41420-022-01142-x","journal-title":"Cell Death Discov"},{"key":"6766_CR10","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1007\/s00592-014-0675-y","volume":"52","author":"G Sebastiani","year":"2015","unstructured":"Sebastiani G, Po A, Miele E et al (2015) MicroRNA-124a is hyperexpressed in type 2 diabetic human pancreatic islets and negatively regulates insulin secretion. Acta Diabetol 52:523\u2013530. https:\/\/doi.org\/10.1007\/s00592-014-0675-y","journal-title":"Acta Diabetol"},{"key":"6766_CR11","doi-asserted-by":"publisher","first-page":"e0188980","DOI":"10.1371\/journal.pone.0188980","volume":"12","author":"P de Candia","year":"2017","unstructured":"de Candia P, Spinetti G, Specchia C et al (2017) A unique plasma microRNA profile defines type 2 diabetes progression. PLoS One 12:e0188980. https:\/\/doi.org\/10.1371\/journal.pone.0188980","journal-title":"PLoS One"},{"key":"6766_CR12","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.2337\/dc13-1847","volume":"37","author":"FJ Ortega","year":"2014","unstructured":"Ortega FJ, Mercader JM, Moreno-Navarrete JM et al (2014) Profiling of circulating microRNAs reveals common microRNAs linked to type 2 diabetes that change with insulin sensitization. Diabetes Care 37:1375\u20131383. https:\/\/doi.org\/10.2337\/dc13-1847","journal-title":"Diabetes Care"},{"key":"6766_CR13","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1039\/c6mb00596a","volume":"13","author":"YO Nunez Lopez","year":"2016","unstructured":"Nunez Lopez YO, Garufi G, Seyhan AA (2016) Altered levels of circulating cytokines and microRNAs in lean and obese individuals with prediabetes and type 2 diabetes. Mol Biosyst 13:106\u2013121. https:\/\/doi.org\/10.1039\/c6mb00596a","journal-title":"Mol Biosyst"},{"key":"6766_CR14","doi-asserted-by":"publisher","first-page":"6872635","DOI":"10.1155\/2018\/6872635","volume":"2018","author":"G Catanzaro","year":"2018","unstructured":"Catanzaro G, Besharat ZM, Chiacchiarini M et al (2018) Circulating micrornas in elderly type 2 diabetic patients. Int J Endocrinol 2018:6872635. https:\/\/doi.org\/10.1155\/2018\/6872635","journal-title":"Int J Endocrinol"},{"key":"6766_CR15","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1080\/13813455.2017.1396349","volume":"124","author":"S Ghorbani","year":"2018","unstructured":"Ghorbani S, Mahdavi R, Alipoor B et al (2018) Decreased serum microRNA-21 level is associated with obesity in healthy and type 2 diabetic subjects. Arch Physiol Biochem 124:300\u2013305. https:\/\/doi.org\/10.1080\/13813455.2017.1396349","journal-title":"Arch Physiol Biochem"},{"key":"6766_CR16","doi-asserted-by":"publisher","first-page":"e3000603","DOI":"10.1371\/journal.pbio.3000603","volume":"18","author":"H Xu","year":"2020","unstructured":"Xu H, Du X, Xu J et al (2020) Pancreatic \u03b2 cell microRNA-26a alleviates type 2 diabetes by improving peripheral insulin sensitivity and preserving \u03b2 cell function. PLoS Biol 18:e3000603. https:\/\/doi.org\/10.1371\/journal.pbio.3000603","journal-title":"PLoS Biol"},{"key":"6766_CR17","doi-asserted-by":"publisher","first-page":"e73272","DOI":"10.1371\/journal.pone.0073272","volume":"8","author":"Y Rong","year":"2013","unstructured":"Rong Y, Bao W, Shan Z et al (2013) Increased microRNA-146a levels in plasma of patients with newly diagnosed type 2 diabetes mellitus. PLoS One 8:e73272. https:\/\/doi.org\/10.1371\/journal.pone.0073272","journal-title":"PLoS One"},{"key":"6766_CR18","doi-asserted-by":"publisher","first-page":"31479","DOI":"10.1038\/srep31479","volume":"6","author":"AA Seyhan","year":"2016","unstructured":"Seyhan AA, Nunez Lopez YO, Xie H et al (2016) Pancreas-enriched miRNAs are altered in the circulation of subjects with diabetes: a pilot cross-sectional study. Sci Rep 6:31479. https:\/\/doi.org\/10.1038\/srep31479","journal-title":"Sci Rep"},{"key":"6766_CR19","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1161\/CIRCRESAHA.110.226357","volume":"107","author":"A Zampetaki","year":"2010","unstructured":"Zampetaki A, Kiechl S, Drozdov I et al (2010) Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes. Circ Res 107:810\u2013817. https:\/\/doi.org\/10.1161\/CIRCRESAHA.110.226357","journal-title":"Circ Res"},{"key":"6766_CR20","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.trsl.2022.03.008","volume":"247","author":"GE Grieco","year":"2022","unstructured":"Grieco GE, Besharat ZM, Licata G et al (2022) Circulating microRNAs as clinically useful biomarkers for type 2 diabetes mellitus: miRNomics from bench to bedside. Transl Res 247:137\u2013157. https:\/\/doi.org\/10.1016\/j.trsl.2022.03.008","journal-title":"Transl Res"},{"key":"6766_CR21","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1016\/j.molmet.2017.06.020","volume":"6","author":"MP LaPierre","year":"2017","unstructured":"LaPierre MP, Stoffel M (2017) MicroRNAs as stress regulators in pancreatic beta cells and diabetes. Mol Metab 6:1010\u20131023. https:\/\/doi.org\/10.1016\/j.molmet.2017.06.020","journal-title":"Mol Metab"},{"key":"6766_CR22","doi-asserted-by":"publisher","first-page":"bqad040","DOI":"10.1210\/endocr\/bqad040","volume":"164","author":"A Karagiannopoulos","year":"2023","unstructured":"Karagiannopoulos A, Cowan E, Eliasson L (2023) miRNAs in the beta cell \u2013 friends or foes? Endocrinology 164:bqad040. https:\/\/doi.org\/10.1210\/endocr\/bqad040","journal-title":"Endocrinology"},{"key":"6766_CR23","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1017\/S2040174422000137","volume":"13","author":"MV Joglekar","year":"2022","unstructured":"Joglekar MV, Kunte PS, Wong WKM et al (2022) Circulating microRNAs from early childhood and adolescence are associated with pre-diabetes at 18 years of age in women from the PMNS cohort. J Dev Orig Health Dis 13:806\u2013811. https:\/\/doi.org\/10.1017\/S2040174422000137","journal-title":"J Dev Orig Health Dis"},{"key":"6766_CR24","doi-asserted-by":"publisher","first-page":"2622","DOI":"10.1038\/s41591-025-03730-7","volume":"31","author":"MV Joglekar","year":"2025","unstructured":"Joglekar MV, Wong WKM, Kunte PS et al (2025) A microRNA-based dynamic risk score for type 1 diabetes. Nat Med 31:2622\u20132631. https:\/\/doi.org\/10.1038\/s41591-025-03730-7","journal-title":"Nat Med"},{"key":"6766_CR25","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.2337\/dc12-0811","volume":"36","author":"G Muscogiuri","year":"2013","unstructured":"Muscogiuri G, Mezza T, Prioletta A et al (2013) Removal of duodenum elicits GLP-1 secretion. Diabetes Care 36:1641\u20131646. https:\/\/doi.org\/10.2337\/dc12-0811","journal-title":"Diabetes Care"},{"key":"6766_CR26","doi-asserted-by":"publisher","first-page":"100606","DOI":"10.1016\/j.xpro.2021.100606","volume":"2","author":"GE Grieco","year":"2021","unstructured":"Grieco GE, Sebastiani G, Fignani D et al (2021) Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples. STAR Protocols 2:100606. https:\/\/doi.org\/10.1016\/j.xpro.2021.100606","journal-title":"STAR Protocols"},{"key":"6766_CR27","doi-asserted-by":"publisher","first-page":"101591","DOI":"10.1016\/j.xcrm.2024.101591","volume":"5","author":"G Sebastiani","year":"2024","unstructured":"Sebastiani G, Grieco GE, Bruttini M et al (2024) A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes. Cell Rep Med 5:101591. https:\/\/doi.org\/10.1016\/j.xcrm.2024.101591","journal-title":"Cell Rep Med"},{"key":"6766_CR28","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1007\/s00125-003-1169-6","volume":"46","author":"E Ferrannini","year":"2003","unstructured":"Ferrannini E, Gastaldelli A, Miyazaki Y et al (2003) Predominant role of reduced beta-cell sensitivity to glucose over insulin resistance in impaired glucose tolerance. Diabetologia 46:1211\u20131219. https:\/\/doi.org\/10.1007\/s00125-003-1169-6","journal-title":"Diabetologia"},{"key":"6766_CR29","doi-asserted-by":"publisher","first-page":"3027","DOI":"10.1210\/clinem\/dgae376","volume":"109","author":"D Redling","year":"2024","unstructured":"Redling D, Bialak S, El Ghormli L et al (2024) Circulating microRNAs as predictors of beta cell function in youth-onset type 2 diabetes: the TODAY study. J Clin Endocrinol Metab 109:3027\u20133035. https:\/\/doi.org\/10.1210\/clinem\/dgae376","journal-title":"J Clin Endocrinol Metab"},{"key":"6766_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s12276-018-0194-y","volume":"50","author":"R Jim\u00e9nez-Lucena","year":"2018","unstructured":"Jim\u00e9nez-Lucena R, Camargo A, Alcal\u00e1-Diaz JF et al (2018) A plasma circulating miRNAs profile predicts type 2 diabetes mellitus and prediabetes: from the CORDIOPREV study. Exp Mol Med 50:1\u201312. https:\/\/doi.org\/10.1038\/s12276-018-0194-y","journal-title":"Exp Mol Med"},{"key":"6766_CR31","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.metabol.2014.12.003","volume":"64","author":"C Higuchi","year":"2015","unstructured":"Higuchi C, Nakatsuka A, Eguchi J et al (2015) Identification of circulating miR-101, miR-375 and miR-802 as biomarkers for type 2 diabetes. Metab Clin Exp 64:489\u2013497. https:\/\/doi.org\/10.1016\/j.metabol.2014.12.003","journal-title":"Metab Clin Exp"},{"key":"6766_CR32","doi-asserted-by":"publisher","first-page":"14274","DOI":"10.1038\/s41598-018-32274-9","volume":"8","author":"A Jaeger","year":"2018","unstructured":"Jaeger A, Zollinger L, Saely CH et al (2018) Circulating microRNAs -192 and -194 are associated with the presence and incidence of diabetes mellitus. Sci Rep 8:14274. https:\/\/doi.org\/10.1038\/s41598-018-32274-9","journal-title":"Sci Rep"},{"key":"6766_CR33","doi-asserted-by":"publisher","first-page":"130","DOI":"10.4239\/wjd.v14.i3.130","volume":"14","author":"PV Dludla","year":"2023","unstructured":"Dludla PV, Mabhida SE, Ziqubu K et al (2023) Pancreatic \u03b2-cell dysfunction in type 2 diabetes: implications of inflammation and oxidative stress. World J Diabetes 14:130\u2013146. https:\/\/doi.org\/10.4239\/wjd.v14.i3.130","journal-title":"World J Diabetes"},{"issue":"Suppl 4","key":"6766_CR34","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1111\/j.1463-1326.2008.00946.x","volume":"10","author":"A Mari","year":"2008","unstructured":"Mari A, Ferrannini E (2008) Beta-cell function assessment from modelling of oral tests: an effective approach. Diabetes Obes Metab 10(Suppl 4):77\u201387. https:\/\/doi.org\/10.1111\/j.1463-1326.2008.00946.x","journal-title":"Diabetes Obes Metab"},{"key":"6766_CR35","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1210\/jc.2004-1133","volume":"90","author":"E Ferrannini","year":"2005","unstructured":"Ferrannini E, Gastaldelli A, Miyazaki Y et al (2005) \u03b2-Cell function in subjects spanning the range from normal glucose tolerance to overt diabetes: a new analysis. J Clin Endocrinol Metab 90:493\u2013500. https:\/\/doi.org\/10.1210\/jc.2004-1133","journal-title":"J Clin Endocrinol Metab"},{"key":"6766_CR36","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.tem.2023.02.001","volume":"34","author":"G Di Giuseppe","year":"2023","unstructured":"Di Giuseppe G, Ciccarelli G, Soldovieri L et al (2023) First-phase insulin secretion: can its evaluation direct therapeutic approaches? Trends Endocrinol Metab 34:216\u2013230. https:\/\/doi.org\/10.1016\/j.tem.2023.02.001","journal-title":"Trends Endocrinol Metab"},{"key":"6766_CR37","doi-asserted-by":"publisher","first-page":"2090","DOI":"10.2337\/dc09-2261","volume":"33","author":"M Manco","year":"2010","unstructured":"Manco M, Panunzi S, Macfarlane DP et al (2010) One-hour plasma glucose identifies insulin resistance and beta-cell dysfunction in individuals with normal glucose tolerance: cross-sectional data from the Relationship between Insulin Sensitivity and Cardiovascular Risk (RISC) study. Diabetes Care 33:2090\u20132097. https:\/\/doi.org\/10.2337\/dc09-2261","journal-title":"Diabetes Care"},{"key":"6766_CR38","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.2337\/dc24-2832","volume":"48","author":"M Abdul-Ghani","year":"2025","unstructured":"Abdul-Ghani M, Abu-Farha M, Abdul-Ghani T et al (2025) One-hour plasma glucose predicts the progression from normal glucose tolerance to prediabetes. Diabetes Care 48:1273\u20131279. https:\/\/doi.org\/10.2337\/dc24-2832","journal-title":"Diabetes Care"},{"key":"6766_CR39","doi-asserted-by":"publisher","first-page":"5589","DOI":"10.3892\/etm.2017.5254","volume":"14","author":"Y Shen","year":"2017","unstructured":"Shen Y, Xu H, Pan X et al (2017) miR-34a and miR-125b are upregulated in peripheral blood mononuclear cells from patients with type 2 diabetes mellitus. Exp Ther Med 14:5589\u20135596. https:\/\/doi.org\/10.3892\/etm.2017.5254","journal-title":"Exp Ther Med"},{"key":"6766_CR40","doi-asserted-by":"publisher","first-page":"2886","DOI":"10.1080\/15384101.2020.1827509","volume":"19","author":"Q Ji","year":"2020","unstructured":"Ji Q, Han J, Wang L et al (2020) MicroRNA-34a promotes apoptosis of retinal vascular endothelial cells by targeting SIRT1 in rats with diabetic retinopathy. Cell Cycle 19:2886\u20132896. https:\/\/doi.org\/10.1080\/15384101.2020.1827509","journal-title":"Cell Cycle"},{"key":"6766_CR41","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1055\/s-0043-125324","volume":"127","author":"D Zhao","year":"2019","unstructured":"Zhao D, Wang N-S, Chen F et al (2019) Intravenous injection of miR-34a inhibitor alleviates diabetes mellitus-induced vascular endothelial dysfunction by targeting NOTCH1. Exp Clin Endocrinol Diabetes 127:255\u2013262. https:\/\/doi.org\/10.1055\/s-0043-125324","journal-title":"Exp Clin Endocrinol Diabetes"},{"key":"6766_CR42","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.1038\/s41418-017-0047-6","volume":"25","author":"I Fomison-Nurse","year":"2018","unstructured":"Fomison-Nurse I, Saw EEL, Gandhi S et al (2018) Diabetes induces the activation of pro-ageing miR-34a in the heart, but has differential effects on cardiomyocytes and cardiac progenitor cells. Cell Death Differ 25:1336\u20131349. https:\/\/doi.org\/10.1038\/s41418-017-0047-6","journal-title":"Cell Death Differ"},{"key":"6766_CR43","doi-asserted-by":"publisher","first-page":"978","DOI":"10.2337\/db09-0881","volume":"59","author":"E Roggli","year":"2010","unstructured":"Roggli E, Britan A, Gattesco S et al (2010) Involvement of microRNAs in the cytotoxic effects exerted by proinflammatory cytokines on pancreatic beta-cells. Diabetes 59:978\u2013986. https:\/\/doi.org\/10.2337\/db09-0881","journal-title":"Diabetes"},{"key":"6766_CR44","doi-asserted-by":"publisher","first-page":"2728","DOI":"10.2337\/db07-1252","volume":"57","author":"P Lovis","year":"2008","unstructured":"Lovis P, Roggli E, Laybutt DR et al (2008) Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction. Diabetes 57:2728\u20132736. https:\/\/doi.org\/10.2337\/db07-1252","journal-title":"Diabetes"},{"key":"6766_CR45","doi-asserted-by":"publisher","first-page":"6654","DOI":"10.1002\/jcp.27406","volume":"234","author":"X-W Tang","year":"2019","unstructured":"Tang X-W, Qin Q-X (2019) miR-335-5p induces insulin resistance and pancreatic islet \u03b2-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-\u03b2 signaling pathway. J Cell Physiol 234:6654\u20136666. https:\/\/doi.org\/10.1002\/jcp.27406","journal-title":"J Cell Physiol"},{"key":"6766_CR46","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.bbrc.2021.04.011","volume":"558","author":"G Li","year":"2021","unstructured":"Li G, Zhang L (2021) miR-335-5p aggravates type 2 diabetes by inhibiting SLC2A4 expression. Biochem Biophys Res Commun 558:71\u201378. https:\/\/doi.org\/10.1016\/j.bbrc.2021.04.011","journal-title":"Biochem Biophys Res Commun"},{"key":"6766_CR47","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.2337\/db17-1238","volume":"67","author":"W Wang","year":"2018","unstructured":"Wang W, Yang C, Wang XY et al (2018) MicroRNA-129 and -335 promote diabetic wound healing by inhibiting Sp1-mediated MMP-9 expression. Diabetes 67:1627\u20131638. https:\/\/doi.org\/10.2337\/db17-1238","journal-title":"Diabetes"},{"key":"6766_CR48","doi-asserted-by":"publisher","first-page":"e18613","DOI":"10.1371\/journal.pone.0018613","volume":"6","author":"JLS Esguerra","year":"2011","unstructured":"Esguerra JLS, Bolmeson C, Cilio CM, Eliasson L (2011) Differential glucose-regulation of microRNAs in pancreatic islets of non-obese type 2 diabetes model Goto-Kakizaki rat. PLoS ONE 6:e18613. https:\/\/doi.org\/10.1371\/journal.pone.0018613","journal-title":"PLoS ONE"},{"key":"6766_CR49","doi-asserted-by":"publisher","first-page":"e13493","DOI":"10.14814\/phy2.13493","volume":"5","author":"VA Salunkhe","year":"2017","unstructured":"Salunkhe VA, Ofori JK, Gandasi NR et al (2017) MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles. Physiol Rep 5:e13493. https:\/\/doi.org\/10.14814\/phy2.13493","journal-title":"Physiol Rep"},{"key":"6766_CR50","doi-asserted-by":"publisher","first-page":"e75348","DOI":"10.1371\/journal.pone.0075348","volume":"8","author":"P de Candia","year":"2013","unstructured":"de Candia P, Torri A, Gorletta T et al (2013) Intracellular modulation, extracellular disposal and serum increase of MiR-150 mark lymphocyte activation. PLoS One 8:e75348. https:\/\/doi.org\/10.1371\/journal.pone.0075348","journal-title":"PLoS One"}],"container-title":["Diabetologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00125-026-06766-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00125-026-06766-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00125-026-06766-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:30:01Z","timestamp":1785472201000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00125-026-06766-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,6]]},"references-count":50,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["6766"],"URL":"https:\/\/doi.org\/10.1007\/s00125-026-06766-7","relation":{},"ISSN":["0012-186X","1432-0428"],"issn-type":[{"value":"0012-186X","type":"print"},{"value":"1432-0428","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,6]]},"assertion":[{"value":"11 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"We acknowledge the help of S. Auddino (Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy) for the analysis of small RNA sequencing data.Parts of this work overlap with the PhD thesis of E. Aiello: \u2018Deciphering islets and circulating microRNAs signature to explore new putative key regulators of glucose tolerance and beta cell dysfunction in type 2 diabetes\u2019, University of Siena, 2025.","order":1,"name":"Ethics","label":"Acknowledgements","group":{"name":"EthicsHeading","label":"Ethics"}},{"value":"Raw and analysed data are available from the corresponding author upon request, after publication.","order":2,"name":"Ethics","label":"Data availability","group":{"name":"EthicsHeading","label":"Ethics"}},{"value":"This work is supported by the Italian Ministry of Health through \u2018Bando Ricerca Finalizzata 2018\u2019 GR-2018-12365577: \u2018The study of human pancreatic islet cell plasticity to predict diabetes onset, progression and personalise therapy\u2019. GS and TM are also supported by the Italian Ministry of University and Research (PNRR-PRIN2022 no. P2022EB5B8 and PRIN2022 no. 2022FRBXHY). FD was supported by the Italian Ministry of University and Research through project PNC 0000001 D3\u00a04 Health, the National Plan for Complementary Investments to the PNRR, funded by NextGeneration EU, by the Italian Ministry of Health project \u2018Multidisciplinary and Interregional Hub for Research and Clinical Experimentation To Combat Pandemics and Antibiotic Resistance\u2019 (project T4-AN-07, PAN-HUB), by the European Union (EU) within the Italian Ministry of University and Research PNRR \u2018National Center for Gene Therapy and Drugs based on RNA Technology\u2019 (project no. CN00000041 CN3 Spoke #5 \u2018Inflammatory and Infectious Diseases\u2019). AG is supported by the Italian Ministry of University and Research (PNRR-PRIN2022 Prot. P2022445ES; PRIN Prot. 2022TJWNLL, PNRR-MCNT2-2023-12378096).","order":3,"name":"Ethics","label":"Funding","group":{"name":"EthicsHeading","label":"Ethics"}},{"value":"The authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.","order":4,"name":"Ethics","label":"Authors\u2019 relationships and activities","group":{"name":"EthicsHeading","label":"Ethics"}},{"value":"EA and GS contributed to the conceptualisation, supervision and coordination of the study and the design of the methodology, conducted the investigation and implemented the bioinformatics and statistical analysis, composed the figures and wrote and reviewed the manuscript. GEG, MB, GG, DF, GDG, GC, AD and MP conducted the experiments and the analyses, interpreted the data and critically revised the manuscript. GQ and SA contributed to the investigation and to the design of the methodology. AG, TM and GD contributed to the investigation and to the design of the methodology, helped acquire funding for the research, and reviewed the manuscript. AM and RB contributed to the investigation and to the design of the methodology, and critically revised the manuscript for important intellectual content. FD and GS contributed to the conceptualisation, supervision and coordination of the study and the design of the methodology, wrote and reviewed the manuscript. and helped acquire funding for the research. All authors substantially contributed to the conception or design of the work, or the acquisition, analysis or interpretation of the data. All authors approved the final version. FD, GS, TM and GD are the guarantors of this work, and, as such, had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of their analysis.","order":5,"name":"Ethics","label":"Contribution statement","group":{"name":"EthicsHeading","label":"Ethics"}}]}}