{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:21:00Z","timestamp":1789730460330,"version":"4.0.1"},"reference-count":9,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":10,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100009757","name":"National Institute of Advanced Industrial Science and Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009757","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Bull Environ Contam Toxicol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Urban stormwater runoff introduces tire\u2011related contaminants such as 6PPD\u2011quinone (6PPD\u2011Q), which has been shown to exhibit acute toxicity in some salmonid species, although the underlying toxic mechanisms remain incompletely understood. To expand in vitro toxicological assessments, we evaluated the cytotoxicity of 3,4\u2011dichloroaniline and 6PPD\u2011quinone using six fish\u2011derived cell lines from salmonids and phylogenetically distant species, based on nominal concentrations at the time of addition. Among these cell lines, CSE\u2011119 cells, which are derived from coho salmon, exhibited the highest sensitivity to 6PPD\u2011quinone (EC\n                    <jats:sub>50<\/jats:sub>\n                    = 8.54\u00a0\u00b5g\/L), demonstrating pronounced species\u2011specific differences in sensitivity to quinone\u2011type toxicants and underscoring the value of comparative in vitro models. Using multiple fish cell lines enables the efficient, animal\u2011welfare\u2013conscious screening of tire\u2011associated chemicals. However, further investigation is needed into evaluation methods that take into account factors such as the stability of chemicals in the culture medium.\n                  <\/jats:p>","DOI":"10.1007\/s00128-026-04314-y","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:22:50Z","timestamp":1786436570000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Toxicity Assessment of Tire-related Chemicals in Cells from Diverse Fish Species"],"prefix":"10.1007","volume":"117","author":[{"given":"Akihiro","family":"Moriyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Horie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2064-7170","authenticated-orcid":false,"given":"Wataru","family":"Naito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"4314_CR1","doi-asserted-by":"publisher","first-page":"107374","DOI":"10.1016\/j.envint.2022.107374","volume":"166","author":"S Di","year":"2022","unstructured":"Di S, Liu Z, Zhao H, Li Y, Qi P, Wang Z, Xu H, Jin Y, Wang X (2022) Chiral perspective evaluations: enantioselective hydrolysis of 6PPD and 6PPD-Quinone in water and enantioselective toxicity to Gobiocypris rarus and Oncorhynchus mykiss. Environ Int 166:107374. https:\/\/doi.org\/10.1016\/j.envint.2022.107374","journal-title":"Environ Int"},{"key":"4314_CR2","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1021\/acs.estlett.3c00196","volume":"10","author":"JB Greer","year":"2023","unstructured":"Greer JB, Dalsky EM, Lane RF, Hansen JD (2023) Establishing an in vitro model to assess the toxicity of 6PPD-quinone and other tire wear transformation products. Environ Sci Technol Lett 10:533\u2013537. https:\/\/doi.org\/10.1021\/acs.estlett.3c00196","journal-title":"Environ Sci Technol Lett"},{"key":"4314_CR3","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1021\/acs.estlett.2c00431","volume":"9","author":"H Mahoney","year":"2022","unstructured":"Mahoney H, da Silva Junior FC, Roberts C, Schultz M, Ji X, Alcaraz AJ, Brinkmann M (2022) Exposure to the tire rubber-derived contaminant 6PPD-quinone causes mitochondrial dysfunction in vitro. Environ Sci Technol Lett 9:765\u2013771. https:\/\/doi.org\/10.1021\/acs.estlett.2c00431","journal-title":"Environ Sci Technol Lett"},{"key":"4314_CR4","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1007\/s00128-025-04150-6","volume":"115","author":"H Mano","year":"2025","unstructured":"Mano H, Moriyama A, Hara J, Tai R, Naito W (2025) Acute toxicities of three para-phenylenediamine quinones to Coho Salmon (Oncorhynchus kisutch) Juveniles and embryonic cells. Bull Environ Contam Toxicol 115:78. https:\/\/doi.org\/10.1007\/s00128-025-04150-6","journal-title":"Bull Environ Contam Toxicol"},{"key":"4314_CR5","doi-asserted-by":"publisher","first-page":"21071","DOI":"10.1021\/acs.est.3c06891","volume":"57","author":"D Montgomery","year":"2023","unstructured":"Montgomery D, Ji X, Cantin J, Philibert D, Foster G, Selinger S, Jain N, Miller J, McIntyre J, de Jourdan B, Wiseman S, Hecker M, Brinkmann M (2023) Interspecies differences in 6PPD-quinone toxicity across seven fish species: Metabolite identification and semi-quantification. Environ Sci Technol Lett 57:21071\u201321079. https:\/\/doi.org\/10.1021\/acs.est.3c06891","journal-title":"Environ Sci Technol Lett"},{"key":"4314_CR6","doi-asserted-by":"publisher","unstructured":"Nair P, Barrett H, Tanoto K, Xie L, Sun J, Yang D, Yao H, Song D, Peng H (2025) Structure and toxicity characterization of alkyl hydroxylated metabolites of 6PPD-Q. Environ Sci Technol 59(15):7474\u20137484. https:\/\/doi.org\/10.1021\/acs.est.4c11823","DOI":"10.1021\/acs.est.4c11823"},{"key":"4314_CR7","doi-asserted-by":"publisher","DOI":"10.1787\/c66d5190-en","volume-title":"Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay","author":"OECD","year":"2021","unstructured":"OECD (2021) Test No. 249: Fish cell line acute toxicity\u2014the RTgill-W1 cell line assay. OECD Publishing, Paris. https:\/\/doi.org\/10.1787\/c66d5190-en"},{"key":"4314_CR8","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/s10616-022-00560-0","volume":"75","author":"A Solhaug","year":"2023","unstructured":"Solhaug A, Gjessing M, Sandvik M, Eriksen GS (2023) The gill epithelial cell lines RTgill-W1, from rainbow trout and ASG-10, from Atlantic salmon, exert different toxicity profiles towards rotenone. Cytotechnology 75:63\u201375. https:\/\/doi.org\/10.1007\/s10616-022-00560-0","journal-title":"Cytotechnology"},{"key":"4314_CR9","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1126\/science.abd6951","volume":"371","author":"Z Tian","year":"2021","unstructured":"Tian Z, Peter KT, Gipe AD, Zhao H, Hou F, Wark DA, Khangaonkar T, Kolodziej E (2021) P. A ubiquitous tire rubber\u2013derived chemical induces acute mortality in coho salmon. Science 371:185\u2013189. https:\/\/doi.org\/10.1126\/science.abd6951","journal-title":"Science"}],"container-title":["Bulletin of Environmental Contamination and Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00128-026-04314-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00128-026-04314-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00128-026-04314-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:08:16Z","timestamp":1787670496000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00128-026-04314-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":9,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["4314"],"URL":"https:\/\/doi.org\/10.1007\/s00128-026-04314-y","relation":{},"ISSN":["0007-4861","1432-0800"],"issn-type":[{"value":"0007-4861","type":"print"},{"value":"1432-0800","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"6 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"40"}}