{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T15:55:55Z","timestamp":1768319755948,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,12,24]],"date-time":"2009-12-24T00:00:00Z","timestamp":1261612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002803","name":"Fondazione Cariplo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Knee surg. sports traumatol. arthrosc."],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Research in tissue engineering has been focused on articular cartilage repair for more than a decade. Some pioneristic studies involved the use of hydrogels such as alginate and fibrin glue which still possess valuable potential for cartilage regeneration. One of the main issues in cartilage tissue engineering is represented by the ideal maturation of the construct, before in vivo implantation, in order to optimize matrix quality and integration. The present study was focused on the effect of in vitro culture on a fibrin glue hydrogel embedding swine chondrocytes. We performed an evaluation of the immunohistochemical and biochemical composition and of the biomechanical properties of the construct after 1 and 5 weeks of culture. We noticed that chondrocytes survived in the fibrin glue gel and enhanced their synthetic activity. In fact, DNA content remained stable, while all indices of cartilage matrix production increased (GAGs content, immunohistochemistry for collagen II and safranin\u2010o staining). On the other hand, the biomechanical properties remained steady, indicating a gradual substitution of the hydrogel scaffold by cartilaginous matrix. This demonstrates that an optimal preculture could provide the surgeon with a better engineered cartilage for implantation. However, whether this more mature tissue will result in a more efficient regeneration of the articular surface still has to be evaluated in future investigations.<\/jats:p>","DOI":"10.1007\/s00167-009-1014-7","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T12:44:37Z","timestamp":1261572277000},"page":"1400-1406","source":"Crossref","is-referenced-by-count":43,"title":["Effect of in vitro culture on a chondrocyte\u2010fibrin glue hydrogel for cartilage repair"],"prefix":"10.1002","volume":"18","author":[{"given":"Celeste","family":"Scotti","sequence":"first","affiliation":[{"name":"Residency Program in Orthopaedics and Traumatology Gaetano Pini Orthopaedic Institute Universit\u00e0 degli Studi di Milano Piazza A. Ferrari 1 20122 Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mangiavini","sequence":"additional","affiliation":[{"name":"Residency Program in Orthopaedics and Traumatology Orthopaedics and Traumatology Clinics Hospital San Gerardo University of Milano Bicocca Via Donizetti 106 20052 Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Boschetti","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Structures Mechanics Department of Structural Engineering Politecnico Di Milano Piazza Leonardo da Vinci, 32 20133 Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Vitari","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences and Technologies for Food Safety Faculty of Veterinary Medicine Universit\u00e0 degli Studi di Milano Via Trentacoste 2 20134 Milan 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