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The analysis of the ionizing radiation tolerance of the chemolithoautotrophic, obligate anaerobic, hyperthermophilic Crenarchaeon <jats:italic>Ignicoccus hospitalis<\/jats:italic> showed a <jats:italic>D<\/jats:italic><jats:sub>10<\/jats:sub>-value of 4.7\u00a0kGy, fourfold exceeding the doses previously determined for other extremophilic archaea. The genome integrity of <jats:italic>I. hospitalis<\/jats:italic> after \u03b3-ray exposure in relation to its survival was visualized by RAPD and qPCR. Furthermore, the discrimination between reproduction, and ongoing metabolic activity was possible for the first time indicating that a potential viable but non-culturable (VBNC) state may also account for <jats:italic>I. hospitalis<\/jats:italic>.<\/jats:p>","DOI":"10.1007\/s00203-020-02125-1","type":"journal-article","created":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T22:02:26Z","timestamp":1608069746000},"page":"1299-1308","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Questioning the radiation limits of life: Ignicoccus hospitalis between replication and VBNC"],"prefix":"10.1007","volume":"203","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7699-0563","authenticated-orcid":false,"given":"Dagmar","family":"Koschnitzki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Moeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Leuko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartos","family":"Przybyla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Beblo-Vranesevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Wirth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Huber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Rachel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Rettberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,15]]},"reference":[{"key":"2125_CR1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S1383-5718(02)00216-4","volume":"521","author":"FA Atienzar","year":"2002","unstructured":"Atienzar FA, Venier P, Jha AN, Depledge MH (2002) Evaluation of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations. 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