{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T03:08:53Z","timestamp":1768273733914,"version":"3.49.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T00:00:00Z","timestamp":1617408000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T00:00:00Z","timestamp":1617408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1007\/s00203-021-02263-0","type":"journal-article","created":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T13:02:42Z","timestamp":1617454962000},"page":"3125-3133","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genome sequencing and comparative genome analysis of 6 hypervirulent Klebsiella pneumoniae strains isolated in China"],"prefix":"10.1007","volume":"203","author":[{"given":"Ling","family":"Du","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kewen","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyue","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongshuang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5191-4887","authenticated-orcid":false,"given":"Yingli","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingfu","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,4,3]]},"reference":[{"key":"2263_CR1","doi-asserted-by":"publisher","first-page":"e00253","DOI":"10.1128\/mSphere.00253-16","volume":"1","author":"BM Carignan","year":"2016","unstructured":"Carignan BM, Brumfield KD, Son MS (2016) Single nucleotide polymorphisms in regulator-encoding genes have an additive effect on virulence gene expression in a Vibrio cholerae clinical isolate. mSphere 1:e00253-e316","journal-title":"mSphere"},{"key":"2263_CR2","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1080\/21505594.2017.1317412","volume":"8","author":"JC Catal\u00e1n-N\u00e1jera","year":"2017","unstructured":"Catal\u00e1n-N\u00e1jera JC, Garza-Ramos U, Barrios-Camacho H (2017) Hypervirulence and hypermucoviscosity: two different but complementary Klebsiella spp. phenotypes? Virulence 8:1111\u20131123","journal-title":"Virulence"},{"key":"2263_CR3","doi-asserted-by":"publisher","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","volume":"44","author":"L Chen","year":"2016","unstructured":"Chen L, Zheng D, Liu B, Yang J, Jin Q (2016) VFDB 2016: hierarchical and refined dataset for big data analysis\u201310 years on. Nucleic Acids Res 44:D694-697","journal-title":"Nucleic Acids Res"},{"key":"2263_CR4","doi-asserted-by":"publisher","first-page":"930","DOI":"10.1093\/cid\/cis565","volume":"55","author":"NC Cheng","year":"2012","unstructured":"Cheng NC, Yu YC, Tai HC et al (2012) Recent trend of necrotizing fasciitis in Taiwan: focus on monomicrobial Klebsiella pneumoniae necrotizing fasciitis. Clin Infect Dis 55:930\u2013939","journal-title":"Clin Infect Dis"},{"key":"2263_CR5","doi-asserted-by":"publisher","DOI":"10.1142\/9789812799623_0012","author":"F Chiaromonte","year":"2002","unstructured":"Chiaromonte F, Yap VB, Miller W (2002) Scoring pairwise genomic sequence alignments. Pac Symp Biocomput. https:\/\/doi.org\/10.1142\/9789812799623_0012","journal-title":"Pac Symp Biocomput."},{"key":"2263_CR6","doi-asserted-by":"publisher","first-page":"e11147","DOI":"10.1371\/journal.pone.0011147","volume":"5","author":"AE Darling","year":"2010","unstructured":"Darling AE, Mau B, Perna NT (2010) progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS ONE 5:e11147","journal-title":"PLoS ONE"},{"key":"2263_CR7","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/s13073-020-00824-4","volume":"13","author":"R de Alwis","year":"2021","unstructured":"de Alwis R, Liang L, Taghavian O et al (2021) The identification of novel immunogenic antigens as potential Shigella vaccine components. Genome Med 13:8","journal-title":"Genome Med"},{"key":"2263_CR8","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1186\/s12866-014-0264-9","volume":"14","author":"A de Greeff","year":"2014","unstructured":"de Greeff A, Buys H, Wells JM, Smith HE (2014) A naturally occurring nucleotide polymorphism in the orf2\/folc promoter is associated with Streptococcus suis virulence. BMC Microbiol 14:264","journal-title":"BMC Microbiol"},{"key":"2263_CR9","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1093\/bioinformatics\/btm009","volume":"23","author":"AL Delcher","year":"2007","unstructured":"Delcher AL, Bratke KA, Powers EC, Salzberg SL (2007) Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23:673\u2013679","journal-title":"Bioinformatics"},{"key":"2263_CR10","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/1471-2105-5-113","volume":"5","author":"RC Edgar","year":"2004","unstructured":"Edgar RC (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5:113","journal-title":"BMC Bioinformatics"},{"key":"2263_CR11","doi-asserted-by":"publisher","first-page":"S18","DOI":"10.1093\/infdis\/jiw378","volume":"215","author":"A Gomez-Simmonds","year":"2017","unstructured":"Gomez-Simmonds A, Uhlemann AC (2017) Clinical implications of genomic adaptation and evolution of carbapenem-resistant Klebsiella pneumoniae. J Infect Dis 215:S18-s27","journal-title":"J Infect Dis"},{"key":"2263_CR12","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1093\/infdis\/jiy534","volume":"219","author":"PF Hsieh","year":"2019","unstructured":"Hsieh PF, Lu YR, Lin TL, Lai LY, Wang JT (2019) Klebsiella pneumoniae Type VI secretion system contributes to bacterial competition, cell invasion, type-1 fimbriae expression, and in vivo colonization. J Infect Dis 219:637\u2013647","journal-title":"J Infect Dis"},{"key":"2263_CR13","doi-asserted-by":"publisher","first-page":"3446","DOI":"10.1099\/mic.0.050336-0","volume":"157","author":"CR Hsu","year":"2011","unstructured":"Hsu CR, Lin TL, Chen YC, Chou HC, Wang JT (2011) The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology (Reading) 157:3446\u20133457","journal-title":"Microbiology (Reading)"},{"key":"2263_CR14","doi-asserted-by":"publisher","first-page":"2275","DOI":"10.1007\/s10096-019-03673-1","volume":"38","author":"F Hu","year":"2019","unstructured":"Hu F, Guo Y, Yang Y, Zheng Y, Wu S, Jiang X, Zhu D (2019) Resistance reported from China antimicrobial surveillance network (CHINET) in 2018. Eur J Clin Microbiol Infect Dis 38:2275\u20132281","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2263_CR15","doi-asserted-by":"publisher","first-page":"2397","DOI":"10.1099\/mic.0.26434-0","volume":"149","author":"J Jagnow","year":"2003","unstructured":"Jagnow J, Clegg S (2003) Klebsiella pneumoniae MrkD-mediated biofilm formation on extracellular matrix- and collagen-coated surfaces. Microbiology (Reading) 149:2397\u20132405","journal-title":"Microbiology (Reading)"},{"key":"2263_CR16","doi-asserted-by":"publisher","first-page":"e688","DOI":"10.1016\/j.ijid.2010.01.007","volume":"14","author":"CH Lee","year":"2010","unstructured":"Lee CH, Liu JW, Su LH, Chien CC, Li CC, Yang KD (2010) Hypermucoviscosity associated with Klebsiella pneumoniae-mediated invasive syndrome: a prospective cross-sectional study in Taiwan. Int J Infect Dis 14:e688-692","journal-title":"Int J Infect Dis"},{"key":"2263_CR17","doi-asserted-by":"publisher","first-page":"483","DOI":"10.3389\/fcimb.2017.00483","volume":"7","author":"CR Lee","year":"2017","unstructured":"Lee CR, Lee JH, Park KS et al (2017) Antimicrobial resistance of Hypervirulent Klebsiella pneumoniae: epidemiology, hypervirulence-associated determinants, and resistance mechanisms. Front Cell Infect Microbiol 7:483","journal-title":"Front Cell Infect Microbiol"},{"key":"2263_CR18","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 25:1754\u20131760","journal-title":"Bioinformatics"},{"key":"2263_CR19","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1093\/bioinformatics\/btn025","volume":"24","author":"R Li","year":"2008","unstructured":"Li R, Li Y, Kristiansen K, Wang J (2008) SOAP: short oligonucleotide alignment program. Bioinformatics 24:713\u2013714","journal-title":"Bioinformatics"},{"key":"2263_CR20","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Handsaker B, Wysoker A et al (2009) The Sequence Alignment\/Map format and SAMtools. Bioinformatics 25:2078\u20132079","journal-title":"Bioinformatics"},{"key":"2263_CR21","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.2217\/fmb.14.48","volume":"9","author":"B Li","year":"2014","unstructured":"Li B, Zhao Y, Liu C, Chen Z, Zhou D (2014) Molecular pathogenesis of Klebsiella pneumoniae. Future Microbiol 9:1071\u20131081","journal-title":"Future Microbiol"},{"key":"2263_CR22","doi-asserted-by":"publisher","first-page":"5379","DOI":"10.1128\/AAC.02523-14","volume":"58","author":"YM Liu","year":"2014","unstructured":"Liu YM, Li BB, Zhang YY, Zhang W, Shen H, Li H, Cao B (2014) Clinical and molecular characteristics of emerging hypervirulent Klebsiella pneumoniae bloodstream infections in mainland China. Antimicrob Agents Chemother 58:5379\u20135385","journal-title":"Antimicrob Agents Chemother"},{"key":"2263_CR23","doi-asserted-by":"publisher","first-page":"e0180666","DOI":"10.1371\/journal.pone.0180666","volume":"12","author":"M Luo","year":"2017","unstructured":"Luo M, Yang S, Li X et al (2017) The KP1_4563 gene is regulated by the cAMP receptor protein and controls type 3 fimbrial function in Klebsiella pneumoniae NTUH-K2044. PLoS ONE 12:e0180666","journal-title":"PLoS ONE"},{"key":"2263_CR24","doi-asserted-by":"publisher","first-page":"e1000845","DOI":"10.1371\/journal.ppat.1000845","volume":"6","author":"T Nandi","year":"2010","unstructured":"Nandi T, Ong C, Singh AP et al (2010) A genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence. PLoS Pathog 6:e1000845","journal-title":"PLoS Pathog"},{"key":"2263_CR25","doi-asserted-by":"publisher","first-page":"D206","DOI":"10.1093\/nar\/gkt1226","volume":"42","author":"R Overbeek","year":"2014","unstructured":"Overbeek R, Olson R, Pusch GD et al (2014) The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res 42:D206-214","journal-title":"Nucleic Acids Res"},{"key":"2263_CR26","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1128\/MMBR.00078-15","volume":"80","author":"MK Paczosa","year":"2016","unstructured":"Paczosa MK, Mecsas J (2016) Klebsiella pneumoniae: going on the offense with a strong defense. Microbiol Mol Biol Rev 80:629\u2013661","journal-title":"Microbiol Mol Biol Rev"},{"key":"2263_CR27","doi-asserted-by":"publisher","first-page":"2231","DOI":"10.1128\/JCM.01716-07","volume":"46","author":"YJ Pan","year":"2008","unstructured":"Pan YJ, Fang HC, Yang HC et al (2008) Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype. J Clin Microbiol 46:2231\u20132240","journal-title":"J Clin Microbiol"},{"issue":"3","key":"2263_CR28","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1111\/1751-7915.13370","volume":"12","author":"YJ Pan","year":"2019","unstructured":"Pan YJ, Lin TL, Chen YY et al (2019) Identification of three podoviruses infecting Klebsiella encoding capsule depolymerases that digest specific capsular types. Microb Biotechnol 12(3):472\u2013486","journal-title":"Microb Biotechnol"},{"key":"2263_CR29","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.micres.2018.08.010","volume":"216","author":"D Peng","year":"2018","unstructured":"Peng D, Li X, Liu P et al (2018) Transcriptional regulation of galF by RcsAB affects capsular polysaccharide formation in Klebsiella pneumoniae NTUH-K2044. Microbiol Res 216:70\u201378","journal-title":"Microbiol Res"},{"issue":"6","key":"2263_CR38","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1007\/s10096-011-1396-6","volume":"31","author":"DK Pomakova","year":"2012","unstructured":"Pomakova DK, Hsiao C-B, Beanan JM, Olson R, MacDonald U, Keynan Y, Russo TA (2012) Clinical and phenotypic differences between classic and hypervirulent Klebsiella pneumonia: an emerging and under-recognized pathogenic variant. Eur J Clin Microbiol Infect Dis 31(6):981\u2013989","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2263_CR30","doi-asserted-by":"publisher","first-page":"e00001","DOI":"10.1128\/CMR.00001-19","volume":"32","author":"TA Russo","year":"2019","unstructured":"Russo TA, Marr CM (2019) Hypervirulent Klebsiella pneumoniae. Clin Microbiol Rev 32:e00001-19","journal-title":"Clin Microbiol Rev"},{"key":"2263_CR31","doi-asserted-by":"publisher","first-page":"107","DOI":"10.4161\/viru.22718","volume":"4","author":"AS Shon","year":"2013","unstructured":"Shon AS, Bajwa RP, Russo TA (2013) Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence 4:107\u2013118","journal-title":"Virulence"},{"key":"2263_CR32","doi-asserted-by":"publisher","first-page":"3761","DOI":"10.1128\/JCM.00977-11","volume":"49","author":"LK Siu","year":"2011","unstructured":"Siu LK, Fung CP, Chang FY, Lee N, Yeh KM, Koh TH, Ip M (2011) Molecular typing and virulence analysis of serotype K1 Klebsiella pneumoniae strains isolated from liver abscess patients and stool samples from noninfectious subjects in Hong Kong, Singapore, and Taiwan. J Clin Microbiol 49:3761\u20133765","journal-title":"J Clin Microbiol"},{"key":"2263_CR33","doi-asserted-by":"publisher","first-page":"4492","DOI":"10.1128\/JB.00315-09","volume":"191","author":"KM Wu","year":"2009","unstructured":"Wu KM, Li LH, Yan JJ et al (2009) Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis. J Bacteriol 191:4492\u20134501","journal-title":"J Bacteriol"},{"key":"2263_CR34","doi-asserted-by":"publisher","first-page":"10690","DOI":"10.1073\/pnas.1605869113","volume":"113","author":"Z Wu","year":"2016","unstructured":"Wu Z, Periaswamy B, Sahin O et al (2016) Point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of Campylobacter jejuni. Proc Natl Acad Sci U S A 113:10690\u201310695","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2263_CR35","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1038\/s41579-019-0315-1","volume":"18","author":"KL Wyres","year":"2020","unstructured":"Wyres KL, Lam MMC, Holt KE (2020) Population genomics of Klebsiella pneumoniae. Nat Rev Microbiol 18:344\u2013359","journal-title":"Nat Rev Microbiol"},{"key":"2263_CR36","doi-asserted-by":"publisher","first-page":"4013","DOI":"10.5897\/AJMR11.785","volume":"5","author":"X Xiao","year":"2011","unstructured":"Xiao X, Zhang JL, Zhang QY et al (2011) Two methods for extraction of high-purity genomic DNA from mucoid Gram-negative bacteria. Afr J Microbiol Res 5:4013\u20134018","journal-title":"Afr J Microbiol Res"},{"key":"2263_CR37","doi-asserted-by":"publisher","first-page":"6115","DOI":"10.1128\/AAC.01127-16","volume":"60","author":"Y Zhang","year":"2016","unstructured":"Zhang Y, Zhao C, Wang Q et al (2016) High prevalence of Hypervirulent Klebsiella pneumoniae Infection in China: geographic distribution, clinical characteristics, and antimicrobial resistance. Antimicrob Agents Chemother 60:6115\u20136120","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-021-02263-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-021-02263-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-021-02263-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T22:42:54Z","timestamp":1744152174000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00203-021-02263-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,3]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["2263"],"URL":"https:\/\/doi.org\/10.1007\/s00203-021-02263-0","relation":{},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,3]]},"assertion":[{"value":"14 June 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 February 2021","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 February 2021","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 April 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The study was approved by the Ethics Committee of the Chongqing Medical University Approval. And patients provided written informed consent for publication.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}]}}