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There, they are microbiologically degraded into free estrogens, which can have harmful effects on aquatic wildlife. Here, the degradation of E2-3S in environmental samples taken upstream, downstream and at the effluent of a WWTP was assessed. Sediment and biofilm samples were enriched for E2-3S-degrading microorganisms, yielding a broad diversity of bacterial isolates, including known and novel degraders of estrogens. Since E2-3S-degrading bacteria were also isolated in the sample upstream of the WWTP, the WWTP does not influence the ability of the microbial community to degrade E2-3S.<\/jats:p>","DOI":"10.1007\/s00203-021-02409-0","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T03:37:00Z","timestamp":1622691420000},"page":"4209-4219","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Broad diversity of bacteria degrading 17\u00df-estradiol-3-sulfate isolated from river sediment and biofilm at a wastewater treatment plant discharge"],"prefix":"10.1007","volume":"203","author":[{"given":"Tamara","family":"Mainetti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"Palmisano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1280-3084","authenticated-orcid":false,"given":"Fabio","family":"Rezzonico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-2907","authenticated-orcid":false,"given":"Bla\u017e","family":"Stres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7422-9567","authenticated-orcid":false,"given":"Susanne","family":"Kern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1237-235X","authenticated-orcid":false,"given":"Theo H. 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