{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:48:34Z","timestamp":1787507314118,"version":"build-2736575974"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"the Cabinet Office, Government of Japan"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2021,11]]},"DOI":"10.1007\/s00203-021-02509-x","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T12:51:52Z","timestamp":1629723112000},"page":"5525-5532","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Microalgal culture in animal cell waste medium for sustainable \u2018cultured food\u2019 production"],"prefix":"10.1007","volume":"203","author":[{"given":"Yuji","family":"Haraguchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1578-2610","authenticated-orcid":false,"given":"Tatsuya","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"2509_CR1","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1038\/nbt.3865","volume":"35","author":"I Ajjawi","year":"2017","unstructured":"Ajjawi I, Verruto J, Aqui M, Soriaga LB, Coppersmith J, Kwok K, Peach L, Orchard E, Kalb R, Xu W, Carlson TJ, Francis K, Konigsfeld K, Bartalis J, Schultz A, Lambert W, Schwartz AS, Brown R, Moellering ER (2017) Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator. Nat Biotechnol 35:647\u2013652. https:\/\/doi.org\/10.1038\/nbt.3865","journal-title":"Nat Biotechnol"},{"key":"2509_CR2","doi-asserted-by":"publisher","first-page":"2213","DOI":"10.3389\/fmicb.2018.02213","volume":"9","author":"ET Alori","year":"2018","unstructured":"Alori ET, Babalola OO (2018) Microbial inoculants for improving crop quality and human health in Africa. Front Microbiol 9:2213. https:\/\/doi.org\/10.3389\/fmicb.2018.02213","journal-title":"Front Microbiol"},{"key":"2509_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2019.121850","volume":"291","author":"J Cheng","year":"2019","unstructured":"Cheng J, Zhu Y, Zhang Z, Yang W (2019) Modification and improvement of microalgae strains for strengthening CO2 fixation from coal-fired flue gas in power plants. Bioresour Technol 291:121850. https:\/\/doi.org\/10.1016\/j.biortech.2019.121850","journal-title":"Bioresour Technol"},{"key":"2509_CR4","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1016\/j.biotechadv.2007.02.001","volume":"25","author":"Y Chisti","year":"2007","unstructured":"Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294\u2013306. https:\/\/doi.org\/10.1016\/j.biotechadv.2007.02.001","journal-title":"Biotechnol Adv"},{"key":"2509_CR5","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.biortech.2014.11.080","volume":"184","author":"SY Chiu","year":"2015","unstructured":"Chiu SY, Kao CY, Chen TY, Chang YB, Kuo CM, Lin CS (2015) Culture of microalgal chlorella for biomass and lipid production using wastewater as nutrient resource. Bioresour Technol 184:179\u2013189. https:\/\/doi.org\/10.1016\/j.biortech.2014.11.080","journal-title":"Bioresour Technol"},{"key":"2509_CR6","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1089\/ind.2020.29227.cge","volume":"16","author":"C Gerhardt","year":"2020","unstructured":"Gerhardt C, Suhlmann G, Ziem\u00dfen F, Donnan D, Warschun M, K\u00fchnle HJ (2020) How will cultured meat and meat alternatives disrupt the agricultural and food industry? Ind Biotechnol 16:262\u2013270. https:\/\/doi.org\/10.1089\/ind.2020.29227.cge","journal-title":"Ind Biotechnol"},{"key":"2509_CR7","doi-asserted-by":"publisher","first-page":"3917","DOI":"10.1128\/AEM.00714-13","volume":"79","author":"JA Gregory","year":"2013","unstructured":"Gregory JA, Topol AB, Doerner DZ, Mayfield S (2013) Alga-produced cholera toxin-Pfs25 fusion proteins as oral vaccines. Appl Environ Microbiol 79:3917\u20133925. https:\/\/doi.org\/10.1128\/AEM.00714-13","journal-title":"Appl Environ Microbiol"},{"key":"2509_CR8","doi-asserted-by":"publisher","first-page":"34","DOI":"10.3305\/nh.2015.32.1.9001","volume":"32","author":"G Guti\u00e9rrez-Salme\u00e1n","year":"2015","unstructured":"Guti\u00e9rrez-Salme\u00e1n G, Fabila-Castillo L, Chamorro-Cevallos G (2015) Nutritional and toxicological aspects of Spirulina (Arthrospira). Nutr Hosp 32:34\u201340. https:\/\/doi.org\/10.3305\/nh.2015.32.1.9001","journal-title":"Nutr Hosp"},{"key":"2509_CR9","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1007\/s10529-021-03106-0","volume":"43","author":"Y Haraguchi","year":"2021","unstructured":"Haraguchi Y, Shimizu T (2021) Three-dimensional tissue fabrication system by co-culture of microalgae and animal cells for production of thicker and healthy cultured food. Biotechnol Lett 43:1117\u20131129. https:\/\/doi.org\/10.1007\/s10529-021-03106-0","journal-title":"Biotechnol Lett"},{"key":"2509_CR10","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1038\/nprot.2012.027","volume":"7","author":"Y Haraguchi","year":"2012","unstructured":"Haraguchi Y, Shimizu T, Sasagawa T, Sekine H, Sakaguchi K, Kikuchi T, Sekine W, Sekiya S, Yamato M, Umezu M, Okano T (2012) Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. Nat Protoc 7:850\u2013858. https:\/\/doi.org\/10.1038\/nprot.2012.027","journal-title":"Nat Protoc"},{"key":"2509_CR11","doi-asserted-by":"publisher","first-page":"41594","DOI":"10.1038\/srep41594","volume":"7","author":"Y Haraguchi","year":"2017","unstructured":"Haraguchi Y, Kagawa Y, Sakaguchi K, Matsuura K, Shimizu T, Okano T (2017) Thicker three-dimensional tissue from a \u201csymbiotic recycling system\u201d combining mammalian cells and algae. Sci Rep 7:41594. https:\/\/doi.org\/10.1038\/srep41594","journal-title":"Sci Rep"},{"key":"2509_CR12","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1002\/biot.201200353","volume":"9","author":"R Harun","year":"2014","unstructured":"Harun R, Yip JWS, Thiruvenkadam S, Ghani WAWAK, Cherrington T, Danquah MK (2014) Algal biomass conversion to bioethanol\u2014a step-by-step assessment. Biotechnol J 9:73\u201386. https:\/\/doi.org\/10.1002\/biot.201200353","journal-title":"Biotechnol J"},{"key":"2509_CR13","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.biortech.2012.10.015","volume":"135","author":"SH Ho","year":"2013","unstructured":"Ho SH, Huang SW, Chen CY, Hasunuma T, Kondo A, Chang JS (2013) Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. Bioresour Technol 135:191\u2013198. https:\/\/doi.org\/10.1016\/j.biortech.2012.10.015","journal-title":"Bioresour Technol"},{"key":"2509_CR14","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1016\/j.biortech.2015.09.061","volume":"198","author":"DJ Lee","year":"2015","unstructured":"Lee DJ, Chang JS, Lai JY (2015) Microalgae-microbial fuel cell: a mini review. Bioresour Technol 198:891\u2013895. https:\/\/doi.org\/10.1016\/j.biortech.2015.09.061","journal-title":"Bioresour Technol"},{"key":"2509_CR15","doi-asserted-by":"publisher","first-page":"11941","DOI":"10.1021\/acs.est.5b01614","volume":"49","author":"CS Mattick","year":"2015","unstructured":"Mattick CS, Landis AE, Allenby BR, Genovese NJ (2015) Anticipatory life cycle analysis of in vitro biomass culture for cultured meat production in the United States. Environ Sci Technol 49:11941\u201311949. https:\/\/doi.org\/10.1021\/acs.est.5b01614","journal-title":"Environ Sci Technol"},{"key":"2509_CR16","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2014.33","volume":"3","author":"S McCusker","year":"2014","unstructured":"McCusker S, Buff PR, Yu Z, Fascetti AJ (2014) Amino acid content of selected plant, algae and insect species: a search for alternative protein sources for use in pet foods. J Nutr Sci 3:e39. https:\/\/doi.org\/10.1017\/jns.2014.33","journal-title":"J Nutr Sci"},{"key":"2509_CR17","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1016\/j.tibtech.2016.04.012","volume":"34","author":"BC Mulukutla","year":"2016","unstructured":"Mulukutla BC, Yongky A, Le T, Mashek DG, Hu WS (2016) Regulation of glucose metabolism\u2014a perspective from cell bioprocessing. Trends Biotechnol 34:638\u2013651. https:\/\/doi.org\/10.1016\/j.tibtech.2016.04.012","journal-title":"Trends Biotechnol"},{"key":"2509_CR18","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.biotechadv.2010.08.005","volume":"29","author":"NH Norsker","year":"2011","unstructured":"Norsker NH, Barbosa MJ, Vermu\u00eb MH, Wijffels RH (2011) Microalgal production\u2014a close look at the economics. Biotechnol Adv 29:24\u201327. https:\/\/doi.org\/10.1016\/j.biotechadv.2010.08.005","journal-title":"Biotechnol Adv"},{"key":"2509_CR19","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2941","volume":"36","author":"Y Okamoto","year":"2020","unstructured":"Okamoto Y, Haraguchi Y, Sawamura N, Asahi T, Shimizu T (2020) Mammalian cell cultivation using nutrients extracted from microalgae. Biotechnol Prog 36:e2941. https:\/\/doi.org\/10.1002\/btpr.2941","journal-title":"Biotechnol Prog"},{"key":"2509_CR20","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1111\/tpj.12310","volume":"76","author":"T Osanai","year":"2013","unstructured":"Osanai T, Kuwahara A, Iijima H, Toyooka K, Sato M, Tanaka K, Ikeuchi M, Saito K, Hirai MY (2013) Pleiotropic effect of sigE over-expression on cell morphology, photosynthesis and hydrogen production in Synechocystis sp. PCC 6803. Plant J 76:456\u2013465. https:\/\/doi.org\/10.1111\/tpj.12310","journal-title":"Plant J"},{"key":"2509_CR21","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1111\/nyas.12569","volume":"1328","author":"MJ Post","year":"2014","unstructured":"Post MJ (2014) An alternative animal protein source: cultured beef. Ann N Y Acad Sci 1328:29\u201333. https:\/\/doi.org\/10.1111\/nyas.12569","journal-title":"Ann N Y Acad Sci"},{"key":"2509_CR22","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1111\/j.1467-7652.2010.00503.x","volume":"8","author":"BA Rasala","year":"2010","unstructured":"Rasala BA, Muto M, Lee PA, Jager M, Cardoso RMF, Behnke CA, Kirk P, Hokanson CA, Crea R, Mendez M, Mayfield SP (2010) Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J 8:719\u2013733. https:\/\/doi.org\/10.1111\/j.1467-7652.2010.00503.x","journal-title":"Plant Biotechnol J"},{"key":"2509_CR23","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.nbt.2013.12.002","volume":"32","author":"F Rossi","year":"2015","unstructured":"Rossi F, Olgu\u00edn EJ, Diels L, De Philippis R (2015) Microbial fixation of CO2 in water bodies and in drylands to combat climate change, soil loss and desertification. N Biotechnol 32:109\u2013120. https:\/\/doi.org\/10.1016\/j.nbt.2013.12.002","journal-title":"N Biotechnol"},{"key":"2509_CR24","doi-asserted-by":"publisher","first-page":"1857","DOI":"10.1093\/pcp\/pch196","volume":"45","author":"A Satoh","year":"2004","unstructured":"Satoh A, Kurano N, Harayama S, Miyachi S (2004) Effects of chloramphenicol on photosynthesis, protein profiles and transketolase activity under extremely high CO2 concentration in an extremely-high-CO2-tolerant green microalga, Chlorococcum littorale. Plant Cell Physiol 45:1857\u20131862. https:\/\/doi.org\/10.1093\/pcp\/pch196","journal-title":"Plant Cell Physiol"},{"key":"2509_CR25","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/0168-1656(95)00196-4","volume":"46","author":"M Schneider","year":"1996","unstructured":"Schneider M, Marison IW, von Stockar U (1996) The importance of ammonia in mammalian cell culture. J Biotechnol 46:161\u2013185. https:\/\/doi.org\/10.1016\/0168-1656(95)00196-4","journal-title":"J Biotechnol"},{"key":"2509_CR26","first-page":"106","volume":"60","author":"AK Singh","year":"2014","unstructured":"Singh AK, Singh MP (2014) Importance of algae as a potential source of biofuel. Cell Mol Biol 60:106\u2013109","journal-title":"Cell Mol Biol"},{"key":"2509_CR27","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1700336","volume":"4","author":"Y Song","year":"2018","unstructured":"Song Y, Johnson D, Peng R, Hensley DK, Bonnesen PV, Liang L, Huang J, Yang F, Zhang F, Qiao R, Baddorf AP, Tschaplinski TJ, Engle NL, Hatzell MC, Wu Z, Cullen DA, Meyer HM 3rd, Sumpter BG, Rondinone AJ (2018) A physical catalyst for the electrolysis of nitrogen to ammonia. Sci Adv 4:e1700336. https:\/\/doi.org\/10.1126\/sciadv.1700336","journal-title":"Sci Adv"},{"key":"2509_CR28","doi-asserted-by":"publisher","first-page":"60","DOI":"10.3389\/fmicb.2014.00060","volume":"5","author":"EA Specht","year":"2014","unstructured":"Specht EA, Mayfield SP (2014) Algae-based oral recombinant vaccines. Front Microbiol 5:60. https:\/\/doi.org\/10.3389\/fmicb.2014.00060","journal-title":"Front Microbiol"},{"key":"2509_CR29","doi-asserted-by":"publisher","unstructured":"Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87\u201396.  https:\/\/doi.org\/10.1263\/jbb.101.87","DOI":"10.1263\/jbb.101.87"},{"key":"2509_CR30","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.ymben.2020.06.002","volume":"61","author":"D Sz\u00e9liov\u00e1","year":"2020","unstructured":"Sz\u00e9liov\u00e1 D, Ruckerbauer DE, Galleguillos SN, Petersen LB, Natter K, Hanscho M, Troyer C, Causon T, Schoeny H, Christensen HB, Lee DY, Lewis NE, Koellensperger G, Hann S, Nielsen LK, Borth N, Zanghellini J (2020) What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines. Metab Eng 61:288\u2013300. https:\/\/doi.org\/10.1016\/j.ymben.2020.06.002","journal-title":"Metab Eng"},{"key":"2509_CR31","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.3389\/fmicb.2018.01150","volume":"9","author":"Y Tanabe","year":"2018","unstructured":"Tanabe Y, Hodoki Y, Sano T, Tada K, Watanabe MM (2018) Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aerguinosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front Microb 9:1150. https:\/\/doi.org\/10.3389\/fmicb.2018.01150","journal-title":"Front Microb"},{"key":"2509_CR32","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.biortech.2016.01.091","volume":"206","author":"C Tarayre","year":"2016","unstructured":"Tarayre C, Clercq LD, Charlier R, Michels E, Meers E, Camargo-Valero M, Delvigne F (2016) New perspectives for the design of sustainable bioprocesses for phosphorus recovery from waste. Bioresour Technol 206:264\u2013274. https:\/\/doi.org\/10.1016\/j.biortech.2016.01.091","journal-title":"Bioresour Technol"},{"key":"2509_CR33","doi-asserted-by":"publisher","first-page":"7380","DOI":"10.1038\/s41598-018-25396-7","volume":"8","author":"R Tsujimoto","year":"2018","unstructured":"Tsujimoto R, Kotani H, Yokomizo K, Yamakawa H, Nonaka A, Fujita Y (2018) Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism. Sci Rep 8:7380. https:\/\/doi.org\/10.1038\/s41598-018-25396-7","journal-title":"Sci Rep"},{"key":"2509_CR34","doi-asserted-by":"publisher","first-page":"6117","DOI":"10.1021\/es200130u","volume":"45","author":"HL Tuomisto","year":"2011","unstructured":"Tuomisto HL, Teixeira de Mattos MJ (2011) Environmental impacts of cultured meat production. Environ Sci Technol 45:6117\u20136123. https:\/\/doi.org\/10.1021\/es200130u","journal-title":"Environ Sci Technol"},{"key":"2509_CR35","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1007\/s12010-009-8866-7","volume":"162","author":"L Wang","year":"2010","unstructured":"Wang L, Min M, Li Y, Chen P, Chen Y, Liu Y, Wang Y, Ruan R (2010) Culture of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Appl Biochem Biotechnol 162:1174\u20131186. https:\/\/doi.org\/10.1007\/s12010-009-8866-7","journal-title":"Appl Biochem Biotechnol"},{"key":"2509_CR36","doi-asserted-by":"publisher","first-page":"2659","DOI":"10.1007\/s00253-017-8184-x","volume":"101","author":"JH Wang","year":"2017","unstructured":"Wang JH, Wang JH, Zhang TY, Dao GH, Xu XQ, Wang XX, Hu HY (2017) Microalgae-based advanced municipal wastewater treatment for reuse in water bodies. Appl Microbiol Biotechnol 101:2659\u20132675. https:\/\/doi.org\/10.1007\/s00253-017-8184-x","journal-title":"Appl Microbiol Biotechnol"},{"key":"2509_CR37","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1016\/j.biortech.2018.07.111","volume":"268","author":"Y Ye","year":"2018","unstructured":"Ye Y, Ngo HH, Guo W, Liu Y, Chang SW, Nguyen DD, Liang H, Wang J (2018) A critical review on ammonium recovery from wastewater for sustainable wastewater management. Bioresour Technol 268:749\u2013758. https:\/\/doi.org\/10.1016\/j.biortech.2018.07.111","journal-title":"Bioresour Technol"},{"key":"2509_CR38","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.15252\/embj.201696151","volume":"36","author":"J Zhang","year":"2017","unstructured":"Zhang J, Pavlova NN, Thompson CB (2017) Cancer cell metabolism: the essential role of the nonessential amino acid, glutamine. EMBO J 36:1302\u20131315. https:\/\/doi.org\/10.15252\/embj.201696151","journal-title":"EMBO J"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-021-02509-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-021-02509-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-021-02509-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T12:28:03Z","timestamp":1633868883000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00203-021-02509-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,23]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["2509"],"URL":"https:\/\/doi.org\/10.1007\/s00203-021-02509-x","relation":{},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,23]]},"assertion":[{"value":"17 May 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 August 2021","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 August 2021","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 August 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Tokyo Women\u2019s Medical University was receiving research funds from IntegriCulture Inc.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study does not include any experimental procedure performed on humans or animals.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}