{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:20:14Z","timestamp":1772119214834,"version":"3.50.1"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidad Miguel Hern\u00e1ndez"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Some weeks after the first CoVID-19 outbreak, the World Health Organization published some real-time PCR (qPCR) protocols developed by different health reference centers. These qPCR designs are being used worldwide to detect SARS-CoV-2 in the population, to monitor the prevalence of the virus during the pandemic. Moreover, some of these protocols to detect SARS-CoV-2 have widely been applied to environmental samples for epidemiological surveillance purposes. In the present work, the specificity of these currently used RT-qPCR designs was validated in vitro using SARS-CoV-2 and highly related coronaviral genomic sequences and compared to performance of the commercially available GPS\u2122 CoVID-19 dtec-RT-qPCR Test. Assays performed with SARS-CoV-2-related genomes showed positive amplification when using some of these qPCR methods, indicating they may give SARS-CoV-2 false positives. This finding may be particularly relevant for SARS-CoV-2 monitoring of environmental samples, where an unknown pool of phylogenetically close-related viruses may exist.<\/jats:p>","DOI":"10.1007\/s00203-022-03029-y","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T11:04:01Z","timestamp":1655982241000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Current RT-qPCR to detect SARS-CoV-2 may give positive results for related coronaviruses"],"prefix":"10.1007","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1550-9590","authenticated-orcid":false,"given":"Antonio","family":"Mart\u00ednez-Murcia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adri\u00e1n","family":"Garc\u00eda-Sirera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"3029_CR1","doi-asserted-by":"publisher","first-page":"S71","DOI":"10.1093\/cid\/ciaa513","volume":"71","author":"JR Andrews","year":"2020","unstructured":"Andrews JR, Yu AT, Saha S et al (2020) Environmental surveillance as a tool for identifying high-risk settings for typhoid transmission. Clin Infect Dis 71:S71\u2013S78. https:\/\/doi.org\/10.1093\/cid\/ciaa513","journal-title":"Clin Infect Dis"},{"key":"3029_CR2","unstructured":"Centers for Disease Control and Prevention 2020 (2020) 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Panel Primers and Probes. In: CDC. https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/lab\/virus-requests.html. Accessed 9 Dec 2021"},{"key":"3029_CR3","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1002\/jmv.25700","volume":"92","author":"C Ceraolo","year":"2020","unstructured":"Ceraolo C, Giorgi FM (2020) Genomic variance of the 2019-nCoV coronavirus. 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Pangolin CoV isolate PCoV_GX-P2V (GenBank accession no.: MT072864.1); and hCoV-19\/pangolin\/Guangxi\/P1E\/2017 (GISAID Accession ID: EPI_ISL_410539). WHO primers are publicly available (World Health Organization,\n                      \n                      ). GPS\u2122 CoVID-19 dtec-RT-qPCR Test primer sequences are not publicly available due to privacy restrictions but available from the corresponding author upon reasonable request.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Data availability"}},{"value":"The authors have no conflict of interest to declare.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"415"}}