{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:34:01Z","timestamp":1792647241447,"version":"4.5.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/RLF\/Re-entry\/41\/2015"],"award-info":[{"award-number":["BT\/RLF\/Re-entry\/41\/2015"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1007\/s00203-022-03037-y","type":"journal-article","created":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T09:04:08Z","timestamp":1655715848000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The source of carbon and nitrogen differentially affects the survival of Neisseria meningitidis in macrophages and epithelial cells"],"prefix":"10.1007","volume":"204","author":[{"given":"Poonam","family":"Kanojiya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riya","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8071-2731","authenticated-orcid":false,"given":"Sunil D.","family":"Saroj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"3037_CR1","doi-asserted-by":"publisher","first-page":"e55798","DOI":"10.1371\/journal.pone.0055798","volume":"8","author":"SN Bartley","year":"2013","unstructured":"Bartley SN, Tzeng YL, Heel K et al (2013) Attachment and invasion of Neisseria meningitidis to host cells is related to surface hydrophobicity, bacterial cell size and capsule. PLoS ONE 8:e55798","journal-title":"PLoS ONE"},{"key":"3037_CR2","doi-asserted-by":"publisher","first-page":"2922","DOI":"10.1128\/IAI.00277-16","volume":"84","author":"KA Blanchette","year":"2016","unstructured":"Blanchette KA, Shenoy AT, Milner J et al (2016) Neuraminidase A-exposed galactose promotes Streptococcus pneumoniae biofilm formation during colonization. Infect Immun 84:2922\u20132932","journal-title":"Infect Immun"},{"key":"3037_CR3","doi-asserted-by":"publisher","first-page":"e934","DOI":"10.21769\/BioProtoc.934","volume":"3","author":"CA Brimacombe","year":"2013","unstructured":"Brimacombe CA, Beatty JT (2013) Surface polysaccharide extraction and quantification. Bio-Protoc 3:e934\u2013e934","journal-title":"Bio-Protoc"},{"key":"3037_CR4","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1038\/nrmicro1955","volume":"6","author":"SA Brown","year":"2008","unstructured":"Brown SA, Palmer KL, Whiteley M (2008) Revisiting the host as a growth medium. Nat Rev Microbiol 6:657\u2013666","journal-title":"Nat Rev Microbiol"},{"key":"3037_CR5","doi-asserted-by":"publisher","first-page":"B64","DOI":"10.1016\/j.vaccine.2009.04.061","volume":"27","author":"DA Caugant","year":"2009","unstructured":"Caugant DA, Maiden MC (2009) Meningococcal carriage and disease-population biology and evolution. Vaccine 27:B64\u2013B70","journal-title":"Vaccine"},{"key":"3037_CR6","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1111\/mmi.12007","volume":"86","author":"AC Chien","year":"2012","unstructured":"Chien AC, Zareh SK, Wang YM et al (2012) Changes in the oligomerization potential of the division inhibitor UgtP co-ordinate Bacillus subtilis cell size with nutrient availability. Mol Microbiol 86:594\u2013610","journal-title":"Mol Microbiol"},{"key":"3037_CR7","first-page":"320","volume":"26","author":"M Derkaoui","year":"2016","unstructured":"Derkaoui M, Antunes A, Abdallah JN et al (2016) Transport and catabolism of carbohydrates by Neisseria meningitidis. J Mol Microbiol Biotechnol 26:320\u2013332","journal-title":"J Mol Microbiol Biotechnol"},{"key":"3037_CR8","doi-asserted-by":"publisher","first-page":"5762","DOI":"10.1128\/IAI.73.9.5762-5766.2005","volume":"73","author":"RM Exley","year":"2005","unstructured":"Exley RM, Goodwin L, Mowe E, Shaw J, Smith H, Read RC, Tang CM (2005) Neisseria meningitidis lactate permease is required for nasopharyngeal colonization. Infect Immun 73:5762\u20135766","journal-title":"Infect Immun"},{"key":"3037_CR9","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1128\/jb.43.6.757-761.1942","volume":"43","author":"ID Frantz Jr","year":"1942","unstructured":"Frantz ID Jr (1942) Growth requirements of the meningococcus. J Bacterial 43:757\u2013761","journal-title":"J Bacterial"},{"key":"3037_CR10","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1042\/CS20090513","volume":"118","author":"DJ Hill","year":"2010","unstructured":"Hill DJ, Griffiths NJ, Borodina E et al (2010) Cellular and molecular biology of Neisseria meningitidis colonization and invasive disease. Clin Sci 118:547\u2013564","journal-title":"Clin Sci"},{"key":"3037_CR11","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1007\/s00424-008-0459-8","volume":"456","author":"KK Kalsi","year":"2008","unstructured":"Kalsi KK, Baker EH, Medina RA et al (2008) Apical and basolateral localisation of GLUT2 transporters in human lung epithelial cells. Pfl\u00fcg Arch-Eur J Phy 456:991\u20131003","journal-title":"Pfl\u00fcg Arch-Eur J Phy"},{"key":"3037_CR12","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1111\/cmi.12138","volume":"15","author":"YA Kwaik","year":"2013","unstructured":"Kwaik YA, Bumann D (2013) Microbial quest for food in vivo: \u2018Nutritional virulence\u2019 as an emerging paradigm. Cell Microbiol 15:882\u2013890","journal-title":"Cell Microbiol"},{"key":"3037_CR13","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/bs.ampbs.2015.05.001","volume":"66","author":"JR Laver","year":"2015","unstructured":"Laver JR, Hughes SE, Read RC (2015) Neisserial molecular adaptations to the nasopharyngeal niche. Adv Microb Physiol 66:323\u2013355","journal-title":"Adv Microb Physiol"},{"key":"3037_CR14","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1099\/00221287-147-6-1473","volume":"147","author":"MP Leighton","year":"2001","unstructured":"Leighton MP, Kelly DJ, Williamson MP et al (2001) NMR and enzyme study of the carbon metabolism of Neisseria meningitidis. Microbiology 147:1473\u20131482","journal-title":"Microbiology"},{"key":"3037_CR15","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1146\/annurev.cellbio.16.1.423","volume":"16","author":"AJ Merz","year":"2000","unstructured":"Merz AJ, So M (2000) Interactions of pathogenic neisseriae with epithelial cell membranes. Ann Rev Cell Dev Bio 16:423\u2013457","journal-title":"Ann Rev Cell Dev Bio"},{"key":"3037_CR16","doi-asserted-by":"publisher","first-page":"3769","DOI":"10.1073\/pnas.91.9.3769","volume":"91","author":"X Nassif","year":"1994","unstructured":"Nassif X, Beretti JL, Lowy J, Stenberg P et al (1994) Roles of pilin and PilC in adhesion of Neisseria meningitidis to human epithelial and endothelial cells. Proc Natl Acad Sci 91:3769\u20133773","journal-title":"Proc Natl Acad Sci"},{"key":"3037_CR17","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1111\/j.1365-2958.2003.03947.x","volume":"51","author":"C Pagliarulo","year":"2004","unstructured":"Pagliarulo C, Salvatore P, De Vitis LR et al (2004) Regulation and differential expression of gdhA encoding NADP-specific glutamate dehydrogenase in Neisseria meningitidis clinical isolates. Mol Microbiol 51:1757\u20131772","journal-title":"Mol Microbiol"},{"key":"3037_CR18","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1128\/iai.64.8.3210-3217.1996","volume":"64","author":"RC Read","year":"1996","unstructured":"Read RC, Zimmerli S, Broaddus C et al (1996) The (alpha2\u2013> 8)-linked polysialic acid capsule of group B Neisseria meningitidis modifies multiple steps during interaction with human macrophages. Infect Immun 64:3210\u20133217","journal-title":"Infect Immun"},{"key":"3037_CR19","doi-asserted-by":"publisher","first-page":"114","DOI":"10.3389\/fcimb.2014.00114","volume":"4","author":"C Schoen","year":"2014","unstructured":"Schoen C, Kischkies L, Elias J, Ampattu BJ (2014) Metabolism and virulence in Neisseria meningitidis. Front Cell Infect Microbiol 4:114","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"3037_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006251","volume":"13","author":"S Sigurl\u00e1sd\u00f3ttir","year":"2017","unstructured":"Sigurl\u00e1sd\u00f3ttir S, Engman J, Eriksson OS et al (2017) Host cell-derived lactate functions as an effector molecule in Neisseria meningitidis microcolony dispersal. PLoS Pathog 13(4):e1006251","journal-title":"PLoS Pathog"},{"key":"3037_CR21","first-page":"1","volume":"8","author":"S Sigurl\u00e1sd\u00f3ttir","year":"2018","unstructured":"Sigurl\u00e1sd\u00f3ttir S, Saroj SD, Eriksson O et al (2018) Quantification of Neisseria meningitidis adherence to human epithelial cells by colony counting. Bio Protoc 8:1\u201310","journal-title":"Bio Protoc"},{"key":"3037_CR22","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1006\/mpat.1995.0071","volume":"19","author":"H Smith","year":"1995","unstructured":"Smith H, Parsons NJ, Cole JA (1995) Sialylation of neisserial lipopolysaccharide: a major influence on pathogenicity. Microb Pathog 19:365\u2013377","journal-title":"Microb Pathog"},{"key":"3037_CR23","doi-asserted-by":"publisher","first-page":"6565","DOI":"10.1128\/IAI.69.11.6565-6572.2001","volume":"69","author":"H Smith","year":"2001","unstructured":"Smith H, Yates EA, Cole JA, Parsons NJ (2001) Lactate stimulation of gonococcal metabolism in media containing glucose: mechanism, impact on pathogenicity, and wider implications for other pathogens. Infect Immun 69:6565\u20136572","journal-title":"Infect Immun"},{"key":"3037_CR24","doi-asserted-by":"publisher","first-page":"4190","DOI":"10.1128\/IAI.00117-07","volume":"75","author":"H Smith","year":"2007","unstructured":"Smith H, Tang CM, Exley RM (2007) Effect of host lactate on gonococci and meningococci: new concepts on the role of metabolites in pathogenicity. Infect Immun 75:4190\u20134198","journal-title":"Infect Immun"},{"key":"3037_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2164-7-128","volume":"7","author":"LA Snyder","year":"2006","unstructured":"Snyder LA, Saunders NJ (2006) The majority of genes in the pathogenic Neisseria species are present in non-pathogenic Neisseria lactamica, including those designated as\u2019 virulence genes\u2019. BMC Genomics 7:1\u201311","journal-title":"BMC Genomics"},{"key":"3037_CR26","doi-asserted-by":"crossref","unstructured":"Soriani M (2017) Unraveling Neisseria meningitidis pathogenesis: from functional genomics to experimental models. F1000 6:1228","DOI":"10.12688\/f1000research.11279.1"},{"key":"3037_CR27","doi-asserted-by":"publisher","first-page":"B71","DOI":"10.1016\/j.vaccine.2009.04.070","volume":"27","author":"DS Stephens","year":"2009","unstructured":"Stephens DS (2009) Biology and pathogenesis of the evolutionarily successful, obligate human bacterium Neisseria meningitidis. Vaccine 27:B71\u2013B77","journal-title":"Vaccine"},{"key":"3037_CR28","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1093\/infdis\/167.2.475","volume":"167","author":"DS Stephens","year":"1993","unstructured":"Stephens DS, Spellman PA, Swartley JS (1993) Effect of the (\u03b12\u2192 8)-linked polysialic acid capsule on adherence of Neisseria meningitidis to human mucosal cells. J Infect Dis 167:475\u2013478","journal-title":"J Infect Dis"},{"key":"3037_CR29","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1016\/0006-3002(57)90254-8","volume":"24","author":"L Svennerholm","year":"1957","unstructured":"Svennerholm L (1957) Quantitative estimation of sialic acids: II A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta 24:604\u2013611","journal-title":"Biochim Biophys Acta"},{"key":"3037_CR30","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/S1286-4579(00)00356-7","volume":"2","author":"YL Tzeng","year":"2000","unstructured":"Tzeng YL, Stephens DS (2000) Epidemiology and pathogenesis of Neisseria meningitidis. Microbes Infect 2:687\u2013700","journal-title":"Microbes Infect"},{"key":"3037_CR31","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1128\/CMR.13.1.144","volume":"13","author":"M Van Deuren","year":"2000","unstructured":"Van Deuren M, Brandtzaeg P, van der Meer JW (2000) Update on meningococcal disease with emphasis on pathogenesis and clinical management. Clin Microbiol Rev 13:144\u2013166","journal-title":"Clin Microbiol Rev"},{"key":"3037_CR32","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1038\/nrmicro2097","volume":"7","author":"M Virji","year":"2009","unstructured":"Virji M (2009) Pathogenic neisseriae: surface modulation, pathogenesis and infection control. Nat Rev Microbiol 7:274\u2013286","journal-title":"Nat Rev Microbiol"},{"key":"3037_CR33","doi-asserted-by":"publisher","first-page":"1271","DOI":"10.1111\/j.1365-2958.1992.tb00848.x","volume":"6","author":"M Virji","year":"1992","unstructured":"Virji M, Alexandrescu C, Ferguson DJ, Saunders JR, Moxon ER (1992) Variations in the expression of pili: the effect on adherence of Neisseria meningitidis to human epithelial and endothelial cells. Mol Microbiol 6:1271\u20131279","journal-title":"Mol Microbiol"},{"key":"3037_CR34","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1111\/j.1365-2958.1993.tb00922.x","volume":"10","author":"M Virji","year":"1993","unstructured":"Virji M, Makepeace K, Ferguson DJ, Achtman M, Moxon ER (1993) Meningococcal Opa and Opc proteins: their role in colonization and invasion of human epithelial and endothelial cells. Mol Microbiol 10:499\u2013510","journal-title":"Mol Microbiol"},{"key":"3037_CR35","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1111\/j.1365-2958.1995.mmi_18040741.x","volume":"18","author":"M Virji","year":"1995","unstructured":"Virji M, Makepeace K, Peak IR, Ferguson DJ, Jennings MP, Moxon ER (1995) Opc-and pilus-dependent interactions of meningococci with human endothelial cells: molecular mechanisms and modulation by surface polysaccharides. Mol Microbiol 18:741\u2013754","journal-title":"Mol Microbiol"},{"key":"3037_CR36","doi-asserted-by":"publisher","first-page":"6132","DOI":"10.1128\/IAI.72.10.6132-6138.2004","volume":"72","author":"K Yi","year":"2004","unstructured":"Yi K, Rasmussen AW, Gudlavalleti SK, Stephens DS, Stojiljkovic I (2004) Biofilm formation by Neisseria meningitidis. Infect Immun 72:6132\u20136138","journal-title":"Infect Immun"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-022-03037-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-022-03037-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-022-03037-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T17:11:22Z","timestamp":1658769082000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00203-022-03037-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,20]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["3037"],"URL":"https:\/\/doi.org\/10.1007\/s00203-022-03037-y","relation":{},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,20]]},"assertion":[{"value":"12 February 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 May 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 June 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 June 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors report no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"No animals were used in the experiments. Therefore, ethics approval was not required.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}],"article-number":"404"}}