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The cells were irregular cocci, 0.8\u20131.8\u00a0\u03bcm in diameter, stained Gram-negative and were motile. The strain utilized H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, formate and the primary and secondary alcohols 1-propanol and 2-propanol for methanogenesis, but not acetate, methanol, ethanol, 1-butanol or 2-butanol. Acetate was required as carbon source. The novel isolate grew at 25\u201340\u00a0\u00b0C, pH 6.0\u20137.5 and 0\u20132.5% (w\/v) NaCl. 16S rRNA gene sequence analysis revealed that the strain is affiliated to the genus <jats:italic>Methanofollis<\/jats:italic>. It shows 98.8% sequence similarity to its closest relative <jats:italic>Methanofollis ethanolicus<\/jats:italic>. The G\u2009+\u2009C content is 60.1\u00a0mol%. Based on the data presented here type strain CaP3V-MF-L2A<jats:sup>T<\/jats:sup> (=\u2009DSM 113321<jats:sup>T<\/jats:sup>\u2009=\u2009JCM 39176<jats:sup>T<\/jats:sup>) represents a novel species, <jats:italic>Methanofollis propanolicus<\/jats:italic> sp. nov.<\/jats:p>","DOI":"10.1007\/s00203-022-03152-w","type":"journal-article","created":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T11:02:42Z","timestamp":1660388562000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Methanofollis propanolicus sp. nov., a novel archaeal isolate from a Costa Rican oil well that uses propanol for methane production"],"prefix":"10.1007","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1856-2100","authenticated-orcid":false,"given":"Linda","family":"Dengler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Meier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7444-2408","authenticated-orcid":false,"given":"Felix","family":"Gr\u00fcnberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9771-0348","authenticated-orcid":false,"given":"Annett","family":"Bellack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6367-1221","authenticated-orcid":false,"given":"Reinhard","family":"Rachel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0570-2517","authenticated-orcid":false,"given":"Dina","family":"Grohmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4251-6091","authenticated-orcid":false,"given":"Harald","family":"Huber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,8,13]]},"reference":[{"issue":"6","key":"3152_CR1","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1128\/aem.32.6.781-791.1976","volume":"32","author":"WE Balch","year":"1976","unstructured":"Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. 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