{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:28:49Z","timestamp":1785364129121,"version":"3.55.0"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"DGAPA-PAPIIT","award":["IN218421"],"award-info":[{"award-number":["IN218421"]}]},{"name":"DGAPA-PAPIIT","award":["IN218421"],"award-info":[{"award-number":["IN218421"]}]},{"name":"DGAPA-PAPIIT","award":["IN218421"],"award-info":[{"award-number":["IN218421"]}]},{"name":"Consejo Nacional de Humanidades Ciencias y Tecnolog\u00edas"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Intracellular pathogens like\n                    <jats:italic>Brucella<\/jats:italic>\n                    face challenges during the intraphagocytic adaptation phase, where the modulation of gene expression plays an essential role in taking advantage of stressors to persist inside the host cell. This study aims to explore the expression of antisense\n                    <jats:italic>virB2<\/jats:italic>\n                    RNA strand and related genes under intracellular simulation media. Sense and antisense\n                    <jats:italic>virB2<\/jats:italic>\n                    RNA strands increased expression when nutrient deprivation and acidification were higher, being starvation more determinative. Meanwhile,\n                    <jats:italic>bspB<\/jats:italic>\n                    , one of the T4SS effector genes, exhibited the highest expression during the exposition to pH 4.5 and nutrient abundance. Based on RNA-seq analysis and RACE data, we constructed a regional map depicting the 5' and 3' ends of\n                    <jats:italic>virB2<\/jats:italic>\n                    and the cis-encoded asRNA_0067. Without affecting the CDS or a possible autonomous RBS, we generate the deletion mutant \u0394asRNA_0067, significantly reducing\n                    <jats:italic>virB2<\/jats:italic>\n                    mRNA expression and survival rate. These results suggest that the antisense asRNA_0067 expression is promoted under exposure to the intraphagocytic adaptation phase stressors, and its deletion is associated with a lower transcription of the\n                    <jats:italic>virB2<\/jats:italic>\n                    gene. Our findings illuminate the significance of these RNA strands in modulating the survival strategy of\n                    <jats:italic>Brucella<\/jats:italic>\n                    within the host and emphasize the role of nutrient deprivation in gene expression.\n                  <\/jats:p>","DOI":"10.1007\/s00203-024-03984-8","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T23:42:28Z","timestamp":1717112548000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Increased Brucella abortus asRNA_0067 expression under intraphagocytic stressors is associated with enhanced virB2 transcription"],"prefix":"10.1007","volume":"206","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3557-973X","authenticated-orcid":false,"given":"Adrian","family":"Mu\u00f1oz-Bucio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3067-2719","authenticated-orcid":false,"given":"Beatriz","family":"Arellano-Reynoso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3298-6076","authenticated-orcid":false,"given":"F\u00e9lix J.","family":"Sangari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4495-563X","authenticated-orcid":false,"given":"Rodrigo","family":"Sieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2276-4573","authenticated-orcid":false,"given":"Patricia","family":"Th\u00e9bault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7731-4049","authenticated-orcid":false,"given":"Clara","family":"Espitia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9187-0671","authenticated-orcid":false,"given":"Juan M.","family":"Garc\u00eda Lobo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9415-9394","authenticated-orcid":false,"given":"Asunci\u00f3n","family":"Seoane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0118-2494","authenticated-orcid":false,"given":"Francisco","family":"Su\u00e1rez-G\u00fcemes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"3984_CR1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1042\/BJ20112180","volume":"444","author":"ME Achard","year":"2012","unstructured":"Achard ME, Stafford SL, Bokil NJ, Chartres J, Bernhardt PV, Schembri MA, Sweet MJ, McEwan AG (2012) Copper redistribution in murine macrophages in response to Salmonella infection. J Biochem 444:51\u201357. https:\/\/doi.org\/10.1042\/BJ20112180","journal-title":"J Biochem"},{"key":"3984_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/IAI.00713-17","volume":"86","author":"P Altamirano-Silva","year":"2018","unstructured":"Altamirano-Silva P, Meza-Torres J, Castillo-Zeled\u00f3n A, Ruiz-Villalobos N, Zu\u00f1iga-Pereira AM, Chac\u00f3n-D\u00edaz C, Moreno E, Guzm\u00e1n-Verri C, Chaves-Olarte E (2018) Brucella abortus senses the intracellular environment through the BvrR \/ BvrS two-component system, which allows B. abortus to adapt to its replicative niche. Infect Inmun 86:1\u201315. https:\/\/doi.org\/10.1128\/IAI.00713-17","journal-title":"Infect Inmun"},{"issue":"6","key":"3984_CR3","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1038\/ni1202","volume":"6","author":"B Arellano-Reynoso","year":"2005","unstructured":"Arellano-Reynoso B, Lapaque N, Salcedo S, Briones G, Ciocchini AE, Ugalde R, Moreno E, Moriy\u00f3n I, Gorvel JP (2005) Cyclic \u03b2-1,2-Glucan is a Brucella virulence factor required for intracellular survival. Nat Immunol 6(6):618\u2013625. https:\/\/doi.org\/10.1038\/ni1202","journal-title":"Nat Immunol"},{"issue":"6","key":"3984_CR4","doi-asserted-by":"publisher","first-page":"3702","DOI":"10.1128\/IAI.73.6.3702","volume":"73","author":"BH Bellaire","year":"2005","unstructured":"Bellaire BH, Roop RM II, Cardelli JA (2005) Opsonized virulent Brucella abortus replicates within nonacidic, endoplasmic reticulum-negative, LAMP-1-positive phagosomes in human monocytes. J Microbiol 73(6):3702\u20133713. https:\/\/doi.org\/10.1128\/IAI.73.6.3702","journal-title":"J Microbiol"},{"issue":"2","key":"3984_CR5","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1111\/j.1365-2958.2012.08117.x","volume":"85","author":"CC Caswell","year":"2012","unstructured":"Caswell CC, Gaines JM, Ciborowski P, Smith D, Borchers CH, Roux CM, Sayoud K, Dunman PM, Roop RM II (2012) Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308. Mol Microbiol 85(2):345\u2013360. https:\/\/doi.org\/10.1111\/j.1365-2958.2012.08117.x","journal-title":"Mol Microbiol"},{"issue":"4","key":"3984_CR6","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1084\/jem.20030088","volume":"198","author":"J Celli","year":"2003","unstructured":"Celli J, Chastellier C, Franchini DM, Pizarro-Cerda J, Moreno E, Gorvel JP (2003) Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum. J Exp Med 198(4):545\u2013556. https:\/\/doi.org\/10.1084\/jem.20030088","journal-title":"J Exp Med"},{"key":"3984_CR7","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1128\/9781683670261.ch7","volume":"6","author":"J Celli","year":"2020","unstructured":"Celli J (2020) The intracellular life cycle of Brucella spp. Bact Intracell 6:101\u2013111. https:\/\/doi.org\/10.1128\/9781683670261.ch7","journal-title":"Bact Intracell"},{"key":"3984_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008561","author":"W Cenens","year":"2020","unstructured":"Cenens W, Andrade MO, Llontop E, Alvarez-Martinez CE, Sgro GG, Farah CS (2020) Bactericidal type IV secretion system homeostasis in Xanthomonas citri. PLoS Pathog. https:\/\/doi.org\/10.1371\/journal.ppat.1008561","journal-title":"PLoS Pathog"},{"issue":"6","key":"3984_CR9","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1038\/nrmicro.2017.15","volume":"15","author":"P Chandrangsu","year":"2017","unstructured":"Chandrangsu P, Rensing C, Helmann JD (2017) Metal homeostasis and resistance in bacteria. Nat Rev Microbiol 15(6):338\u2013350. https:\/\/doi.org\/10.1038\/nrmicro.2017.15","journal-title":"Nat Rev Microbiol"},{"key":"3984_CR10","doi-asserted-by":"publisher","first-page":"1088941","DOI":"10.3389\/fmicb.2022.1088941","volume":"13","author":"F Chen","year":"2022","unstructured":"Chen F, Cocaign-Bousquet M, Girbal L, Nouaille S (2022) 5\u2019UTR sequences influence protein levels in Escherichia coli by regulating translation initiation and mRNA stability. Front Microbiol 13:1088941","journal-title":"Front Microbiol"},{"issue":"10","key":"3984_CR11","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1016\/j.tibs.2015.08.005","volume":"40","author":"K Cotter","year":"2016","unstructured":"Cotter K, Stransky L, Mcguire C, Forgac M (2016) Recent insights into the structure, regulation and function of the V-ATPases. Trends Biochem Sci 40(10):611\u2013622. https:\/\/doi.org\/10.1016\/j.tibs.2015.08.005","journal-title":"Trends Biochem Sci"},{"key":"3984_CR12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003402","author":"MF Criscitiello","year":"2013","unstructured":"Criscitiello MF, Dickman MB, Samuel JE, de Figueiredo P (2013) Tripping on acid: trans-kingdom perspectives on biological acids in immunity and pathogenesis. PLoS Pathog. https:\/\/doi.org\/10.1371\/journal.ppat.1003402","journal-title":"PLoS Pathog"},{"issue":"12","key":"3984_CR13","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1016\/j.tim.2015.09.007","volume":"23","author":"X de Bolle","year":"2015","unstructured":"de Bolle X, Crosson S, Matroule JY, Letesson JJ (2015) Brucella abortus cell cycle and infection are coordinated. Trends Microbiol 23(12):812\u2013821. https:\/\/doi.org\/10.1016\/j.tim.2015.09.007","journal-title":"Trends Microbiol"},{"issue":"2","key":"3984_CR14","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.resmic.2004.09.007","volume":"156","author":"G Demarre","year":"2005","unstructured":"Demarre G, Gu\u00e9rout AM, Matsumoto-Mashimo C, Rowe-Magnus DA, Marli\u00e8re P, Mazel D (2005) A new family of mobilizable suicide plasmids based on broad host range R388 plasmid (IncW) and RP4 plasmid (IncP\u03b1) conjugative machineries and their cognate Escherichia coli host strains. Res Microbiol 156(2):245\u2013255. https:\/\/doi.org\/10.1016\/j.resmic.2004.09.007","journal-title":"Res Microbiol"},{"key":"3984_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2021.794535","volume":"12","author":"JA de la Garza-Garc\u00eda","year":"2021","unstructured":"de la Garza-Garc\u00eda JA, Ouahrani-Bettache S, Lyonnais S, Ornelas-Eusebio E, Freddi L, Al Dahouk S, Occhialini A, K\u00f6hler S (2021) Comparative genome-wide transcriptome analysis of Brucella suis and Brucella microti under acid stress at pH 4.5: cold shock protein CspA and Dps are associated with acid resistance of B. microti. Front Microbiol 12:1\u201322. https:\/\/doi.org\/10.3389\/fmicb.2021.794535","journal-title":"Front Microbiol"},{"issue":"2","key":"3984_CR16","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1128\/MMBR.00032-10","volume":"75","author":"J Georg","year":"2011","unstructured":"Georg J, Hess WR (2011) cis-antisense RNA, another level of gene regulation in bacteria. Microbiol Mol Biol Rev 75(2):286\u2013300. https:\/\/doi.org\/10.1128\/MMBR.00032-10","journal-title":"Microbiol Mol Biol Rev"},{"issue":"5","key":"3984_CR17","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","volume":"6","author":"DG Gibson","year":"2009","unstructured":"Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6(5):343\u2013345. https:\/\/doi.org\/10.1038\/nmeth.1318","journal-title":"Nat Methods"},{"issue":"9","key":"3984_CR18","doi-asserted-by":"publisher","first-page":"5143","DOI":"10.1128\/IAI.72.9.5143-5149.2004","volume":"72","author":"AB Den Hartigh","year":"2004","unstructured":"Den Hartigh AB, Sun YH, Sondervan D, Heuvelmans N, Reinders MO, Ficht TA, Tsolis RM (2004) Differential requirements for VirB1 and VirB2 during Brucella abortus infection. Infect Immun 72(9):5143\u20135149. https:\/\/doi.org\/10.1128\/IAI.72.9.5143-5149.2004","journal-title":"Infect Immun"},{"issue":"5","key":"3984_CR19","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.ijmm.2013.04.002","volume":"303","author":"CH Hoe","year":"2013","unstructured":"Hoe CH, Raabe CA, Rozhdestvensky TS, Tang TH (2013) Bacterial sRNAs: regulation in stress. Int J Med Microbiol 303(5):217\u2013229. https:\/\/doi.org\/10.1016\/j.ijmm.2013.04.002","journal-title":"Int J Med Microbiol"},{"key":"3984_CR20","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2015.00072","author":"Y Ke","year":"2015","unstructured":"Ke Y, Wang Y, Li W, Chen Z (2015) Type IV secretion system of Brucella spp and its effectors. FrontCell Infect Microbiol. https:\/\/doi.org\/10.3389\/fcimb.2015.00072","journal-title":"FrontCell Infect Microbiol"},{"key":"3984_CR21","doi-asserted-by":"publisher","first-page":"1026220","DOI":"10.3389\/fvets.2022.1026220","volume":"9","author":"KA King","year":"2022","unstructured":"King KA, Caudill MT, Caswell CC (2022) A comprehensive review of small regulatory RNAs in Brucella spp. Front Vet Sci 9:1026220. https:\/\/doi.org\/10.3389\/fvets.2022.1026220","journal-title":"Front Vet Sci"},{"issue":"10","key":"3984_CR22","doi-asserted-by":"publisher","first-page":"5757","DOI":"10.1093\/nar\/gkx165","volume":"45","author":"CL Kleinman","year":"2017","unstructured":"Kleinman CL, Sycz G, Bonomi HR, Rodriguez RM, Zorreguieta A, Sieira R (2017) ChIP-seq analysis of the LuxR-type regulator VjbR reveals novel insights into the Brucella virulence gene expression network. Nucleic Acids Res 45(10):5757\u20135769. https:\/\/doi.org\/10.1093\/nar\/gkx165","journal-title":"Nucleic Acids Res"},{"key":"3984_CR23","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/S0378-1135(02)00215-8","volume":"90","author":"S K\u00f6hler","year":"2002","unstructured":"K\u00f6hler S, Porte F, Jubier-Maurin V, Ouahrani-Bettache S, Teyssier J, Liautard JP (2002) The intramacrophagic environment of Brucella suis and bacterial response. Vet Microbiol 90:299\u2013309. https:\/\/doi.org\/10.1016\/S0378-1135(02)00215-8","journal-title":"Vet Microbiol"},{"issue":"22","key":"3984_CR24","doi-asserted-by":"publisher","first-page":"8674","DOI":"10.3390\/ijms21228674","volume":"21","author":"MO Koobotse","year":"2020","unstructured":"Koobotse MO, Schmidt D, Holly JM, Perks CM (2020) Glucose concentration in cell culture medium influences the BRCA1-mediated regulation of the lipogenic action of IGF-I in breast cancer cells. Int J Mol Sci 21(22):8674. https:\/\/doi.org\/10.3390\/ijms21228674","journal-title":"Int J Mol Sci"},{"issue":"6","key":"3984_CR25","doi-asserted-by":"publisher","first-page":"1633","DOI":"10.1046\/j.1365-2958.2003.03535.x","volume":"48","author":"SD Lawhon","year":"2003","unstructured":"Lawhon SD, Frye JG, Suyemoto M, Porwollik S, Mcclelland M, Altier C (2003) Global regulation by CsrA in Salmonella typhimurium. Mol Microbiol 48(6):1633\u20131645. https:\/\/doi.org\/10.1046\/j.1365-2958.2003.03535.x","journal-title":"Mol Microbiol"},{"key":"3984_CR500","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.biochi.2019.04.015","volume":"164","author":"M Lejars","year":"2019","unstructured":"Lejars M, Kobayashi A, Hajnsdorf E (2019) Physiological roles of antisense RNAs in prokaryotes. Biochimie 164:3\u201316. https:\/\/doi.org\/10.1016\/j.biochi.2019.04.015","journal-title":"Biochimie"},{"issue":"4","key":"3984_CR26","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394Ct method. Methods 25(4):402\u2013408. https:\/\/doi.org\/10.1006\/meth.2001.1262","journal-title":"Methods"},{"key":"3984_CR27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx279","author":"M Mann","year":"2017","unstructured":"Mann M, Wright PR, Backofen R (2017) IntaRNA 2.0: enhanced and customizable prediction of RNA\u2013RNA interactions. Nucleic Acids Res. https:\/\/doi.org\/10.1093\/nar\/gkx279","journal-title":"Nucleic Acids Res"},{"key":"3984_CR28","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/978-1-4684-5442-0_16","volume":"225","author":"FR Maxfield","year":"1987","unstructured":"Maxfield FR, Yamashiro DJ (1987) Endosome acidification and the pathways of receptor-mediated endocytosis. Adv Exp MedBiol 225:189\u2013198. https:\/\/doi.org\/10.1007\/978-1-4684-5442-0_16","journal-title":"Adv Exp MedBiol"},{"key":"3984_CR501","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.596277","volume":"10","author":"JA Millar","year":"2021","unstructured":"Millar JA, Raghavan R (2021) Modulation of bacterial fitness and virulence through antisense RNAs. Front Cell Infect Microbiol 10:596277. https:\/\/doi.org\/10.3389\/fcimb.2020.596277","journal-title":"Front Cell Infect Microbiol"},{"issue":"3","key":"3984_CR29","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.chom.2017.07.017","volume":"22","author":"CN Miller","year":"2017","unstructured":"Miller CN, Smith EP, Cundiff JA, Knodler LA, Blackburn JB, Lupashin V, Celli J (2017) A Brucella type IV effector targets the COG tethering complex to remodel host secretory traffic and promote intracellular replication. Cell Host Microbe 22(3):317\u2013329. https:\/\/doi.org\/10.1016\/j.chom.2017.07.017","journal-title":"Cell Host Microbe"},{"issue":"1","key":"3984_CR30","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.plasmid.2012.09.002","volume":"69","author":"P Mossey","year":"2013","unstructured":"Mossey P, Das A (2013) Plasmid expression of Agrobacterium tumefaciens octopine Ti-plasmid virB8 gene is regulated by translational coupling. Plasmid 69(1):72\u201380. https:\/\/doi.org\/10.1016\/j.plasmid.2012.09.002","journal-title":"Plasmid"},{"issue":"6","key":"3984_CR31","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.1046\/j.1365-2958.1999.01569.x","volume":"33","author":"D O'Callaghan","year":"1999","unstructured":"O\u2019Callaghan D, Cazevieille C, Allardet-Servent A, Boschiroli ML, Bourg G, Foulongne V, Frutos P, Kulakov Y, Ramuz M (1999) A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis. Mol Microbiol 33(6):1210\u20131220. https:\/\/doi.org\/10.1046\/j.1365-2958.1999.01569.x","journal-title":"Mol Microbiol"},{"issue":"2","key":"3984_CR32","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/s11250-009-9407-7","volume":"6","author":"G Pappas","year":"2006","unstructured":"Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV (2006) The new global map of human brucellosis. Lancet Infect Dis 6(2):91\u201399. https:\/\/doi.org\/10.1007\/s11250-009-9407-7","journal-title":"Lancet Infect Dis"},{"issue":"2","key":"3984_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/femsle\/fnu017","volume":"362","author":"X Peng","year":"2015","unstructured":"Peng X, Dong H, Wu Q (2015) A new cis-encoded sRNA, BsrH, regulating the expression of hemH gene in Brucella abortus 2308. FEMS Microbiol Lett 362(2):1\u20137. https:\/\/doi.org\/10.1093\/femsle\/fnu017","journal-title":"FEMS Microbiol Lett"},{"issue":"3","key":"3984_CR34","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.plasmid.2004.02.003","volume":"51","author":"N Philippe","year":"2004","unstructured":"Philippe N, Alcaraz JP, Coursange E, Geiselmann J, Schneider D (2004) Improvement of PCVD442, a suicide plasmid for gene allele exchange in bacteria. Plasmid 51(3):246\u2013255. https:\/\/doi.org\/10.1016\/j.plasmid.2004.02.003","journal-title":"Plasmid"},{"issue":"8","key":"3984_CR35","doi-asserted-by":"publisher","first-page":"4041","DOI":"10.1128\/iai.67.8.4041-4047.1999","volume":"67","author":"F Porte","year":"1999","unstructured":"Porte F, Liautard JP, K\u00f6hler S (1999) Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages. Infect Immun 67(8):4041\u20134047. https:\/\/doi.org\/10.1128\/iai.67.8.4041-4047.1999","journal-title":"Infect Immun"},{"key":"3984_CR36","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a008755","author":"U Repnik","year":"2013","unstructured":"Repnik U, \u010cesen MH, Turk B (2013) The endolysosomal system in cell death and survival. Cold Spring Harb Perspect Biol. https:\/\/doi.org\/10.1101\/cshperspect.a008755","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"9","key":"3984_CR37","doi-asserted-by":"publisher","first-page":"e0274397","DOI":"10.1371\/journal.pone.0274397","volume":"17","author":"O Rivas-Solano","year":"2022","unstructured":"Rivas-Solano O, Van der Henst M, Castillo-Zeled\u00f3n A, Su\u00e1rez-Esquivel M, Mu\u00f1oz-Vargas L, Capitan-Barrios Z, Thomson NR, Chaves-Olarte E, Moreno E, De Bolle X, Guzm\u00e1n-Verri C (2022) The regulon of Brucella abortus two-component system BvrR\/BvrS reveals the coordination of metabolic pathways required for intracellular life. PLoS ONE 17(9):e0274397. https:\/\/doi.org\/10.1371\/journal.pone.0274397","journal-title":"PLoS ONE"},{"key":"3984_CR38","doi-asserted-by":"publisher","unstructured":"Sansen J, Th\u00e9bault P, Dutour I, Bourqui R (2016) Visualization of sRNA-mRNA interaction predictions. Proceedings of the International Conference on Information Visualisation pp 342\u201347. https:\/\/doi.org\/10.1109\/IV.2016.14","DOI":"10.1109\/IV.2016.14"},{"issue":"9","key":"3984_CR39","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.2217\/fmb.13.83","volume":"8","author":"R Sieira","year":"2013","unstructured":"Sieira R (2013) Regulation of virulence in Brucella: an eclectic repertoire of transcription factors defines the complex architecture of the VirB Promoter. Future Microbiol 8(9):1193\u20131208. https:\/\/doi.org\/10.2217\/fmb.13.83","journal-title":"Future Microbiol"},{"issue":"1","key":"3984_CR40","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1128\/JB.01124-09","volume":"192","author":"R Sieira","year":"2010","unstructured":"Sieira R, Arocena M, Bukata L, Comerci DJ, Ugalde RA (2010) Metabolic control of virulence genes in Brucella abortus: HutC coordinates VirB expression and the histidine utilization pathway by direct binding to both promoters. J Bacteriol 192(1):217\u2013224. https:\/\/doi.org\/10.1128\/JB.01124-09","journal-title":"J Bacteriol"},{"issue":"11","key":"3984_CR41","doi-asserted-by":"publisher","first-page":"4890","DOI":"10.1093\/nar\/gkr037","volume":"39","author":"D Stazic","year":"2011","unstructured":"Stazic D, Lindell D, Steglich C (2011) Antisense RNA protects mRNA from RNase E degradation by RNA-RNA duplex formation during phage infection. Nucleic Acids Res 39(11):4890\u20134899. https:\/\/doi.org\/10.1093\/nar\/gkr037","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"3984_CR42","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/1471-2105-15-39","volume":"15","author":"E Vaes","year":"2014","unstructured":"Vaes E, Khan M, Mombaerts P (2014) Statistical analysis of differential gene expression relative to a fold change threshold on NanoString data of mouse odorant receptor genes. BMC Bioinform 15(1):16. https:\/\/doi.org\/10.1186\/1471-2105-15-39","journal-title":"BMC Bioinform"},{"key":"3984_CR43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06851.x","author":"Q Wang","year":"2009","unstructured":"Wang Q, Harshey RM (2009) Rcs signalling-activated transcription of rcsA induces strong anti-sense transcription of upstream fliPQR flagellar genes from a weak intergenic promoter: regulatory roles for the anti-sense transcript in virulence and motility. Mol Microbiol. https:\/\/doi.org\/10.1111\/j.1365-2958.2009.06851.x","journal-title":"Mol Microbiol"},{"key":"3984_CR44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005368","author":"Y Wang","year":"2009","unstructured":"Wang Y, Chen Z, Qiao F, Ying T, Yuan J, Zhong Z, Zhou L, Du X, Wang Z, Zhao J, Dong S, Jia L, Yuan X, Yang R, Sun Y, Huang L (2009) Comparative proteomics analyses reveal the VirB of B. melitensis affects expression of intracellular survival related proteins. PLoS ONE. https:\/\/doi.org\/10.1371\/journal.pone.0005368","journal-title":"PLoS ONE"},{"key":"3984_CR45","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2015.00164","volume":"6","author":"Y Wang","year":"2015","unstructured":"Wang Y, Ke Y, Xu J, Wang L, Wang T, Liang H, Zhang W, Gong C, Yuan J, Zhuang Y, An C, Lei S, Du X, W, Li W, Yuan X, Huang L, Yang X, Chen Z (2015) Identification of a novel small non-coding RNA modulating the intracellular survival of Brucella melitensis. Front Microbiol 6:1\u201312. https:\/\/doi.org\/10.3389\/fmicb.2015.00164","journal-title":"Front Microbiol"},{"key":"3984_CR46","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.ijmm.2019.04.002","volume":"309","author":"Y Wang","year":"2019","unstructured":"Wang Y, Ke Y, Duan C, Ma X, Hao Q, Song L (2019) A small non-coding RNA facilitates Brucella melitensis intracellular survival by regulating the expression of virulence factor. Int J Med Microbiol 309:225\u2013231. https:\/\/doi.org\/10.1016\/j.ijmm.2019.04.002","journal-title":"Int J Med Microbiol"},{"key":"3984_CR47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fbioe.2021.758561","volume":"9","author":"Y Wang","year":"2021","unstructured":"Wang Y, Yue X, Yuan S, Hong Y, Hu W, Li Y (2021) Internal promoters and their effects on the transcription of operon genes for epothilone production in Myxococcus xanthus. Front Bioeng Biotechnol 9:1\u201311. https:\/\/doi.org\/10.3389\/fbioe.2021.758561","journal-title":"Front Bioeng Biotechnol"},{"issue":"4","key":"3984_CR48","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1016\/j.cell.2009.01.043","volume":"136","author":"LS Waters","year":"2009","unstructured":"Waters LS, Storz G (2009) Regulatory RNAs in bacteria. Cell 136(4):615\u2013628. https:\/\/doi.org\/10.1016\/j.cell.2009.01.043","journal-title":"Cell"},{"key":"3984_CR49","doi-asserted-by":"publisher","DOI":"10.21037\/ncri.2019.10.02","author":"D Watkins","year":"2019","unstructured":"Watkins D, Arya DP (2019) Regulatory roles of small RNAs in prokaryotes: parallels and contrast with eukaryotic miRNA. Non-Coding RNA Investig. https:\/\/doi.org\/10.21037\/ncri.2019.10.02","journal-title":"Non-Coding RNA Investig"},{"issue":"6","key":"3984_CR50","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1080\/15548627.2019.1569916","volume":"15","author":"Y Xia","year":"2019","unstructured":"Xia Y, Liu N, Xie X, Bi G, Ba H, Li L, Zhang J, Deng X, Yao Y, Tang Z (2019) The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion. Autophagy 15(6):960\u2013975. https:\/\/doi.org\/10.1080\/15548627.2019.1569916","journal-title":"Autophagy"},{"key":"3984_CR51","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222413637","author":"X Xiong","year":"2021","unstructured":"Xiong X, Li B, Zhou Z, Gu G, Li M, Liu J, Jiao J (2021) The VirB system plays a crucial role in Brucella intracellular infection. Int J Mol Sci. https:\/\/doi.org\/10.3390\/ijms222413637","journal-title":"Int J Mol Sci"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-024-03984-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-024-03984-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-024-03984-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T09:10:06Z","timestamp":1718615406000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00203-024-03984-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["3984"],"URL":"https:\/\/doi.org\/10.1007\/s00203-024-03984-8","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3976901\/v1","asserted-by":"object"}]},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,31]]},"assertion":[{"value":"21 February 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"On behalf of all authors, the corresponding author states that there is no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"285"}}