{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:02:26Z","timestamp":1789444946502,"version":"build-2803163510"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1007\/s00203-024-04120-2","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T05:02:15Z","timestamp":1725598935000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cold-adapted live attenuated MERS-CoV vaccine strain remains attenuated in mice after multiple passages in Vero cells at 37\u00a0\u00b0C"],"prefix":"10.1007","volume":"206","author":[{"given":"Heejeong","family":"Seo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyueng","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"issue":"1","key":"4120_CR1","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1186\/s12917-023-03769-z","volume":"19","author":"M Abdelazim","year":"2023","unstructured":"Abdelazim M, Abdelkader R, Ali A, Shahein MA et al (2023) A longitudinal study of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels. BMC Vet Res 19(1):228","journal-title":"BMC Vet Res"},{"key":"4120_CR2","doi-asserted-by":"crossref","unstructured":"Abeyagunawardena IA, Dharmaratne SD (2023) A comparison of transmissibility of SARS-CoV-2 variants of concern. Virology Journal (2023) 20:59","DOI":"10.1186\/s12985-023-02018-x"},{"key":"4120_CR3","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1093\/cid\/ciu359","volume":"201459","author":"MM Al-Abdallat","year":"2014","unstructured":"Al-Abdallat MM, Payne DC, Alqasrawi S, Rha B et al (2014) Hospital-asso ciated outbreak of Middle East respiratory syndrome coronavirus: a serologic, epidemiologic, and clinical description. Clin Infect Dis 201459:1225\u20131233","journal-title":"Clin Infect Dis"},{"issue":"10","key":"4120_CR4","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.5455\/OVJ.2023.v13.i10.7","volume":"13","author":"HSR Al-Taee","year":"2023","unstructured":"Al-Taee HSR, Sekhi AA, Gharban HAJ, Biati HMA (2023) Serological identification of MERS-CoV in camels of Wasit Province, Iraq. Open Vet J 13(10):1283\u20131289","journal-title":"Open Vet J"},{"issue":"3","key":"4120_CR5","first-page":"362","volume":"4","author":"MM Ali","year":"2024","unstructured":"Ali MM, Fathelrahman E, El Awad AI et al (2024) Epidemiology and scenario simulations of the Middle East Respiratory Syndrome Corona Virus (MERS-CoV) Disease Spread and Control for Dromedary Camels in United Arab Emirates (UAE). Anim (Basel) 4(3):362","journal-title":"Anim (Basel)"},{"key":"4120_CR6","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1056\/NEJMsr1408795","volume":"376","author":"YM Arabi","year":"2017","unstructured":"Arabi YM, Balkhy HH, Hayden FG, Bouchama A et al (2017) Middle East respiratory syndrome. N Engl J Med 376:584\u2013594","journal-title":"N Engl J Med"},{"issue":"16","key":"4120_CR7","doi-asserted-by":"publisher","first-page":"8651","DOI":"10.1128\/JVI.00614-15","volume":"89","author":"A Asisa Volz","year":"2015","unstructured":"Asisa Volz A, Alexandra Kupke A, Fei Song F et al (2015) Protective efficacy of recombinant modified Vaccinia Virus Ankara delivering Middle East Respiratory Syndrome Coronavirus Spike glycoprotein. J Virol 89(16):8651\u20138656","journal-title":"J Virol"},{"key":"4120_CR8","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1056\/NEJMoa1306742","volume":"369","author":"A Assiri","year":"2013","unstructured":"Assiri A, McGeer A, Perl TM, Price CS et al (2013) Hospital outbreak of Middle East respiratory syndrome coronavirus. N Engl J Med 369:407\u2013441","journal-title":"N Engl J Med"},{"key":"4120_CR9","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1016\/j.idc.2019.08.001","volume":"33","author":"EI Azhar","year":"2019","unstructured":"Azhar EI, Hui DSC, Memish ZA, Drosten C, Zumla A (2019) The Middle East respiratory syndrome (MERS). Infect Dis Clin North Am 33:891\u2013905","journal-title":"Infect Dis Clin North Am"},{"key":"4120_CR10","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1016\/S1473-3099(20)30160-2","volume":"20","author":"PM Folegatti","year":"2020","unstructured":"Folegatti PM, Bittaye M, Flaxman A et al (2020) Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial. Lancet Infect Dis 20:816\u2013826","journal-title":"Lancet Infect Dis"},{"key":"4120_CR11","doi-asserted-by":"publisher","first-page":"e0165978","DOI":"10.1371\/journal.pone.0165978","volume":"11","author":"MA Garbati","year":"2016","unstructured":"Garbati MA, Fagbo SF, Fang VJ, Skakni L et al (2016) A comparative study of clinical presentation and risk factors for adverse outcome in patients hospitalised with acute respiratory disease due to MERS coronavirus or other causes. PLoS ONE 11:e0165978","journal-title":"PLoS ONE"},{"key":"4120_CR12","doi-asserted-by":"publisher","first-page":"e00103","DOI":"10.1128\/mBio.00103-21","volume":"12","author":"J Gutierrez-Alvarez","year":"2021","unstructured":"Gutierrez-Alvarez J, Honrubia JM, Fernandez-Delgado R et al (2021) Genetically engineered live-attenuated Middle East respiratory syndrome coronavirus viruses confer full protection against lethal infection. mBio 12:e00103\u2013e00121","journal-title":"mBio"},{"key":"4120_CR13","doi-asserted-by":"publisher","first-page":"102078","DOI":"10.1016\/j.cimid.2023.102078","volume":"103","author":"MA Haroun","year":"2023","unstructured":"Haroun MA, Khames M, Fettata S, Khames Y et al (2023) First serological evidence of MERS-CoV in dromedary camels from Algeria. Comp Immunol Microbiol Infect Dis 103:102078","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"4120_CR14","doi-asserted-by":"publisher","first-page":"5742","DOI":"10.1016\/j.vaccine.2020.07.003","volume":"38","author":"A Hashemzadeh","year":"2020","unstructured":"Hashemzadeh A, Avan A, Ferns GA et al (2020) Vaccines based on virus-like nano-particles for use against Middle East respiratory syndrome (MERS) coronavirus. Vaccine 38:5742\u20135746","journal-title":"Vaccine"},{"key":"4120_CR15","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1080\/14728222.2020.1820482","volume":"25","author":"S Jiang","year":"2021","unstructured":"Jiang S, Zhang X, Du L (2021) Therapeutic antibodies and fusion inhibitors targeting the spike protein of SARS-CoV-2. Expert Opin Ther Targets 25:415\u2013421","journal-title":"Expert Opin Ther Targets"},{"key":"4120_CR17","doi-asserted-by":"publisher","first-page":"1244833","DOI":"10.3389\/fvets.2023.1244833","volume":"10","author":"AI Khalafalla","year":"2023","unstructured":"Khalafalla AI (2023) Zoonotic diseases transmitted from the camels. Front Vet Sci 10:1244833","journal-title":"Front Vet Sci"},{"key":"4120_CR16","doi-asserted-by":"publisher","first-page":"1182165","DOI":"10.3389\/fvets.2023.1182165","volume":"10","author":"AI Khalafalla","year":"2023","unstructured":"Khalafalla AI, Ishag HZA, Albalushi HIA, Al-Hammadi ZMA (2023) Isolation and genetic characterization of MERS-CoV from dromedary camels in the United Arab Emirates. Front Vet Sci 10:1182165","journal-title":"Front Vet Sci"},{"key":"4120_CR18","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1016\/S1473-3099(20)30248-6","volume":"20","author":"T Koch","year":"2020","unstructured":"Koch T, Dahlke C, Fathi A et al (2020) Safety and immunogenicity of a modified Vaccinia virus Ankara vector vaccine candidate for Middle East respiratory syndrome: an open-label, phase 1 trial. Lancet Infect Dis 20:827\u2013838","journal-title":"Lancet Infect Dis"},{"key":"4120_CR19","doi-asserted-by":"publisher","first-page":"663","DOI":"10.3390\/v11070663","volume":"11","author":"F Li","year":"2019","unstructured":"Li F, Du L (2019) MERS coronavirus: an emerging zoonotic virus. Viruses 11:663","journal-title":"Viruses"},{"issue":"5","key":"4120_CR20","doi-asserted-by":"publisher","first-page":"712","DOI":"10.1093\/infdis\/jiv499","volume":"213","author":"K Li","year":"2016","unstructured":"Li K, Wohlford-Lenane C, Perlma S et al (2016) Middle East Respiratory Syndrome Coronavirus causes multiple organ damage and Lethal Disease in mice transgenic for human dipeptidyl peptidase 4. J Infect Dis 213(5):712\u2013722","journal-title":"J Infect Dis"},{"key":"4120_CR21","doi-asserted-by":"publisher","first-page":"11654","DOI":"10.1128\/JVI.01815-15","volume":"89","author":"AH Malczyk","year":"2015","unstructured":"Malczyk AH, Kupke A, Pr\u20acufe,r S et al (2015) A highly immunogenic and protective Middle East respiratory syndrome coronavirus vaccine based on a recombinant Measles virus vaccine platform. J Virol 89:11654\u201311667","journal-title":"J Virol"},{"key":"4120_CR22","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1016\/S1473-3099(19)30266-X","volume":"19","author":"K Modjarrad","year":"2019","unstructured":"Modjarrad K, Roberts CC, Mills KT et al (2019) Safety and immunogenicity of an anti-middle East respiratory syndrome coronavirus DNA vaccine: a phase 1, open-label, single-arm, dose escalation trial. Lancet Infect Dis 19:1013\u20131022","journal-title":"Lancet Infect Dis"},{"key":"4120_CR23","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1038\/s41467-023-36754-z","volume":"14","author":"SK Myeni","year":"2023","unstructured":"Myeni SK, Bredenbeek PJ, Knaap RCM, Dalebout TJ et al (2023) Engineering potent live attenuated coronavirus vaccines by targeted inactivation of the immune evasive viral deubiquitinase. Nat Commun 14:1141","journal-title":"Nat Commun"},{"issue":"3","key":"4120_CR24","doi-asserted-by":"publisher","first-page":"581","DOI":"10.3201\/eid3003.231488","volume":"30","author":"BM Ogoti","year":"2024","unstructured":"Ogoti BM, Riitho V, Wildemann J et al (2024) Biphasic MERS-CoV incidence in nomadic dromedaries with putative transmission to humans, Kenya, 2022\u20132023. Emerg Infect Dis 30(3):581\u2013585","journal-title":"Emerg Infect Dis"},{"key":"4120_CR25","first-page":"493","volume":"27","author":"LJ Reed","year":"1938","unstructured":"Reed LJ, Muench H (1938) A simple method of estimating 50% endpoints. Am J Hyg 27:493\u2013497","journal-title":"Am J Hyg"},{"issue":"8","key":"4120_CR26","doi-asserted-by":"publisher","first-page":"1353","DOI":"10.3390\/vaccines11081353","volume":"11","author":"H Seo","year":"2023","unstructured":"Seo H, Jang Y, Kwak D (2023) The protective efficacy of single-dose nasal immunization with Cold-adapted live-attenuated MERS-CoV vaccine against Lethal MERS-CoV infections in mice. Vaccines (Basel) 11(8):1353","journal-title":"Vaccines (Basel)"},{"key":"4120_CR27","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.trsl.2021.11.007","volume":"242","author":"W Tai","year":"2022","unstructured":"Tai W, Zhang X, Yang Y, Zhu J, Du L (2022) Advances in mRNA and other vaccines against MERS-CoV. Transl Res 242:20\u201337","journal-title":"Transl Res"},{"key":"4120_CR28","doi-asserted-by":"publisher","first-page":"199156","DOI":"10.1016\/j.virusres.2023.199156","volume":"334","author":"W Tai","year":"2023","unstructured":"Tai W, Zheng J, Zhang X, Shi J et al (2023) MERS-CoV RBD-mRNA vaccine induces potent and broadly neutralizing antibodies with protection against MERS-CoV infection. Virus Res 334:199156","journal-title":"Virus Res"},{"key":"4120_CR29","doi-asserted-by":"publisher","first-page":"1814","DOI":"10.1056\/NEJMoa1211721","volume":"367","author":"AM Zaki","year":"2012","unstructured":"Zaki AM, van Boheemen S, Bestebroer TM et al (2012) Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 367:1814\u20131820","journal-title":"N Engl J Med"},{"issue":"9","key":"4120_CR30","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1080\/14760584.2020.1813574","volume":"19","author":"N Zhang","year":"2020","unstructured":"Zhang N, Shang J, Li C et al (2020) An overview of Middle East respiratory syndrome coronavirus vaccines in preclinical studies. Expert Rev Vaccines 19(9):817\u2013829","journal-title":"Expert Rev Vaccines"},{"key":"4120_CR31","doi-asserted-by":"publisher","first-page":"60","DOI":"10.3390\/v11010060","volume":"11","author":"Y Zhou","year":"2019","unstructured":"Zhou Y, Yang Y, Huang J et al (2019) Advances in MERS-CoV vaccines and therapeutics based on the receptor-binding domain. Viruses 11:60","journal-title":"Viruses"},{"key":"4120_CR32","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1016\/S0140-6736(15)60454-8","volume":"386","author":"A Zumla","year":"2015","unstructured":"Zumla A, Hui DS, Perlman S (2015) Middle East respiratory syndrome. Lancet 386:995\u20131007","journal-title":"Lancet"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-024-04120-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-024-04120-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-024-04120-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T05:02:25Z","timestamp":1725598945000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00203-024-04120-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,6]]},"references-count":32,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["4120"],"URL":"https:\/\/doi.org\/10.1007\/s00203-024-04120-2","relation":{},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,6]]},"assertion":[{"value":"29 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 August 2024","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 August 2024","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 September 2024","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}],"article-number":"393"}}