{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T21:03:31Z","timestamp":1771275811757,"version":"3.50.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1007\/s00203-025-04554-2","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T13:38:02Z","timestamp":1763127482000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomic and systems biology insights into lead stress tolerance of Pennisetum purpureum bioaugmented with Enterobacter cloacae"],"prefix":"10.1007","volume":"208","author":[{"given":"Anamika","family":"Das","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Sajitha","family":"Lulu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeevanandam","family":"Vaishnavi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manas","family":"Manam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanagavel","family":"Deepankumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jastin","family":"Samuel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jabez W","family":"Osborne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"issue":"12","key":"4554_CR1","doi-asserted-by":"publisher","first-page":"1328","DOI":"10.1021\/acssynbio.6b00256","volume":"5","author":"BL Adams","year":"2016","unstructured":"Adams BL (2016) The next generation of synthetic biology chassis: moving synthetic biology from the laboratory to the field. ACS Synth Biol 5(12):1328\u20131330","journal-title":"ACS Synth Biol"},{"issue":"8","key":"4554_CR2","doi-asserted-by":"publisher","first-page":"558","DOI":"10.3390\/metabo11080558","volume":"11","author":"JW Allwood","year":"2021","unstructured":"Allwood JW, Williams A, Uthe H, van Dam NM, Mur LA, Grant MR, P\u00e9triacq P (2021) Unravelling plant responses to stress\u2014The importance of targeted and untargeted metabolomics. Metabolites 11(8):558","journal-title":"Metabolites"},{"key":"4554_CR3","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/j.gene.2018.01.042","volume":"647","author":"R Anupama","year":"2018","unstructured":"Anupama R, Lulu SS, Mukherjee A, Babu S (2018) Cross-regulatory network in Pseudomonas aeruginosa biofilm genes and TiO2 anatase induced molecular perturbations in key proteins unraveled by a systems biology approach. Gene 647:289\u2013296","journal-title":"Gene"},{"key":"4554_CR4","doi-asserted-by":"publisher","first-page":"e00161","DOI":"10.1016\/j.teac.2022.e00161","volume":"34","author":"C Bedia","year":"2022","unstructured":"Bedia C (2022) Metabolomics in environmental toxicology: applications and challenges. Trends Environ Anal Chem 34:e00161","journal-title":"Trends Environ Anal Chem"},{"key":"4554_CR5","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.cattod.2016.06.003","volume":"279","author":"RM Braga","year":"2017","unstructured":"Braga RM, Melo DM, Sobrinho EV, Barros JM, Melo MA, Carvalho AF, Freitas JC (2017) Catalytic upgrading of elephant grass (Pennisetum purpureum Schum) pyrolysis vapor using WO3 supported on RHA and RHA-MCM-41. Catal Today 279:224\u2013232","journal-title":"Catal Today"},{"issue":"Suppl 4","key":"4554_CR6","doi-asserted-by":"publisher","first-page":"S11","DOI":"10.1186\/1752-0509-8-S4-S11","volume":"8","author":"CH Chin","year":"2014","unstructured":"Chin CH, Chen SH, Wu HH, Ho CW, Ko MT, Lin CY (2014) CytoHubba: identifying hub objects and sub-networks from complex interactome. BMC Syst Biol 8(Suppl 4):S11","journal-title":"BMC Syst Biol"},{"issue":"10","key":"4554_CR7","doi-asserted-by":"publisher","first-page":"2366","DOI":"10.1038\/nprot.2007.324","volume":"2","author":"MS Cline","year":"2007","unstructured":"Cline MS, Smoot M, Cerami E, Kuchinsky A, Landys N, Workman C, Bader GD (2007) Integration of biological networks and gene expression data using cytoscape. Nat Protoc 2(10):2366\u20132382","journal-title":"Nat Protoc"},{"issue":"18","key":"4554_CR8","doi-asserted-by":"publisher","first-page":"15444","DOI":"10.1007\/s11356-017-8988-3","volume":"24","author":"A Das","year":"2017","unstructured":"Das A, Belgaonkar P, Raman AS, Banu S, Osborne JW (2017) Bioremoval of lead using pennisetum purpureum augmented with Enterobacter cloacae-VITPASJ1: A pot culture approach. Environ Sci Pollut Res 24(18):15444\u201315453","journal-title":"Environ Sci Pollut Res"},{"key":"4554_CR9","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.procbio.2023.10.015","volume":"134","author":"P Dey","year":"2023","unstructured":"Dey P, Buragohain T, Osborne WJ (2023) Inhibition of microbial pathogens and toxicity assessment of noformicin synthesized by psychrobacter faecalis: an endophyte of averrhoa Carambola. Process Biochem 134:329\u2013340","journal-title":"Process Biochem"},{"issue":"1","key":"4554_CR10","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.jtcms.2016.11.007","volume":"8","author":"L Du","year":"2021","unstructured":"Du L, Ye X, Li M, Wang H, Zhang B, Zheng R, Wang Y (2021) Mechanisms of traditional Chinese medicines in the treatment of allergic rhinitis using a network biology approach. J Traditional Chin Med Sci 8(1):82\u201389","journal-title":"J Traditional Chin Med Sci"},{"key":"4554_CR11","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.envexpbot.2011.09.001","volume":"75","author":"NE Estrella-G\u00f3mez","year":"2012","unstructured":"Estrella-G\u00f3mez NE, Sauri-Duch E, Zapata-P\u00e9rez O, Santamar\u00eda JM (2012) Glutathione plays a role in protecting leaves of Salvinia minima from Pb2\u2009+\u2009damage associated with changes in the expression of SmGS genes and increased activity of GS. Environ Exp Bot 75:188\u2013194","journal-title":"Environ Exp Bot"},{"issue":"8","key":"4554_CR12","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.1080\/15226514.2024.2313559","volume":"26","author":"K Gajaje","year":"2024","unstructured":"Gajaje K, Ultra Jr VU, Nwaefuna AE, Zhou N (2024) Phytostabilization of fly Ash from a coalmine in Botswana and biovalorisation of the recovered Napier grass (Pennisetum purpureum Schumach). Int J Phytoremediation 26(8):1291\u20131304","journal-title":"Int J Phytoremediation"},{"issue":"2","key":"4554_CR13","doi-asserted-by":"publisher","first-page":"91","DOI":"10.5296\/jbls.v6i2.7152","volume":"6","author":"F Hadi","year":"2015","unstructured":"Hadi F, Aziz T (2015) A mini review on lead (Pb) toxicity in plants. J Biology Life Sci 6(2):91\u2013101","journal-title":"J Biology Life Sci"},{"key":"4554_CR14","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1016\/j.envpol.2012.07.008","volume":"170","author":"M Iqbal","year":"2012","unstructured":"Iqbal M, Puschenreiter M, Oburger E, Santner J, Wenzel WW (2012) Sulfur-aided phytoextraction of cd and Zn by Salix Smithiana combined with in situ metal immobilization by gravel sludge and red mud. Environ Pollut 170:222\u2013231","journal-title":"Environ Pollut"},{"key":"4554_CR15","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.plantsci.2016.11.015","volume":"256","author":"L Jiang","year":"2017","unstructured":"Jiang L, Wang W, Chen Z, Gao Q, Xu Q, Cao H (2017) A role for APX1 gene in lead tolerance in Arabidopsis Thaliana. Plant Sci 256:94\u2013102","journal-title":"Plant Sci"},{"key":"4554_CR16","doi-asserted-by":"publisher","first-page":"113960","DOI":"10.1016\/j.ecoenv.2022.113960","volume":"243","author":"J Jin","year":"2022","unstructured":"Jin J, Song Z, Zhao B, Zhang Y, Wang R (2022) Physiological And metabolomics responses of hydrangea macrophylla (Thunb.) Ser. And hydrangea strigosa Rehd. To lead exposure. Ecotoxicol Environ Saf 243:113960","journal-title":"Ecotoxicol Environ Saf"},{"issue":"3","key":"4554_CR17","doi-asserted-by":"publisher","first-page":"3145","DOI":"10.3390\/ijms13033145","volume":"13","author":"M Jozefczak","year":"2012","unstructured":"Jozefczak M, Remans T, Vangronsveld J, Cuypers A (2012) Glutathione is a key player in metal-induced oxidative stress defenses. Int J Mol Sci 13(3):3145\u20133175","journal-title":"Int J Mol Sci"},{"issue":"1","key":"4554_CR18","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.sjbs.2015.06.008","volume":"23","author":"AY Khalifa","year":"2016","unstructured":"Khalifa AY, Alsyeeh AM, Almalki MA, Saleh FA (2016) Characterization of the plant growth promoting bacterium, Enterobacter cloacae MSR1, isolated from roots of non-nodulating medicago sativa. Saudi J Biol Sci 23(1):79\u201386","journal-title":"Saudi J Biol Sci"},{"key":"4554_CR19","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1016\/j.eti.2017.07.007","volume":"8","author":"R Kotoky","year":"2017","unstructured":"Kotoky R, Das S, Singha LP, Pandey P, Singha KM (2017) Biodegradation of benzo (a) pyrene by biofilm forming and plant growth promoting acinetobacter sp. strain PDB4. Environ Technol Innov 8:256\u2013268","journal-title":"Environ Technol Innov"},{"issue":"suppl1","key":"4554_CR20","doi-asserted-by":"publisher","first-page":"D684","DOI":"10.1093\/nar\/gkm795","volume":"36","author":"M Kuhn","year":"2007","unstructured":"Kuhn M, von Mering C, Campillos M, Jensen LJ, Bork P (2007) STITCH: interaction networks of chemicals and proteins. Nucleic Acids Res 36(suppl1):D684\u2013D688","journal-title":"Nucleic Acids Res"},{"key":"4554_CR21","doi-asserted-by":"publisher","first-page":"1305","DOI":"10.1016\/j.chemosphere.2014.01.068","volume":"119","author":"L Liu","year":"2015","unstructured":"Liu L, Wang Z, Ju F, Zhang T (2015) Co-occurrence correlations of heavy metals in sediments revealed using network analysis. Chemosphere 119:1305\u20131313","journal-title":"Chemosphere"},{"issue":"2","key":"4554_CR22","first-page":"121","volume":"11","author":"FT Mambe","year":"2016","unstructured":"Mambe FT, Voukeng IK, Beng VP, Kuete V (2016) Antibacterial activities of methanol extracts from alchornea cordifolia and four other Cameroonian plants against MDR phenotypes. J Taibah Univ Med Sci 11(2):121\u2013127","journal-title":"J Taibah Univ Med Sci"},{"key":"4554_CR23","doi-asserted-by":"publisher","first-page":"129128","DOI":"10.1016\/j.jhazmat.2022.129128","volume":"436","author":"L Meng","year":"2022","unstructured":"Meng L, Yang Y, Ma Z, Jiang J, Zhang X, Chen Z, Yin X (2022) Integrated physiological, transcriptomic and metabolomic analysis of the response of trifolium pratense L. to Pb toxicity. J Hazard Mater 436:129128","journal-title":"J Hazard Mater"},{"key":"4554_CR24","doi-asserted-by":"publisher","first-page":"794373","DOI":"10.3389\/fpls.2021.794373","volume":"12","author":"A Raza","year":"2022","unstructured":"Raza A, Tabassum J, Zahid Z, Charagh S, Bashir S, Barmukh R, Varshney RK (2022) Advances in omics approaches for improving toxic metals\/metalloids tolerance in plants. Front Plant Sci 12:794373","journal-title":"Front Plant Sci"},{"issue":"3","key":"4554_CR25","doi-asserted-by":"publisher","first-page":"706","DOI":"10.3390\/life13030706","volume":"13","author":"U Salam","year":"2023","unstructured":"Salam U, Ullah S, Tang ZH, Elateeq AA, Khan Y, Khan J, Ali S (2023) Plant metabolomics: an overview of the role of primary and secondary metabolites against different environmental stress factors. Life 13(3):706","journal-title":"Life"},{"issue":"16","key":"4554_CR26","doi-asserted-by":"publisher","first-page":"9440","DOI":"10.1073\/pnas.1530509100","volume":"100","author":"JD Storey","year":"2003","unstructured":"Storey JD, Tibshirani R (2003) Statistical significance for genomewide studies. Proc Natl Acad Sci 100(16):9440\u20139445","journal-title":"Proc Natl Acad Sci"},{"issue":"suppl1","key":"4554_CR27","first-page":"D561","volume":"39","author":"D Szklarczyk","year":"2010","unstructured":"Szklarczyk D, Franceschini A, Kuhn M, Simonovic M, Roth A, Minguez P, Mering CV (2010) The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res 39(suppl1):D561\u2013D568","journal-title":"Nucleic Acids Res"},{"key":"4554_CR29","first-page":"293","volume":"6","author":"Y Wang","year":"2015","unstructured":"Wang Y, Shen H, Xu L, Zhu X, Li C, Zhang W, Liu L (2015) Transport, ultrastructural localization, and distribution of chemical forms of lead in radish (Raphanus sativus L). Front Plant Sci 6:293","journal-title":"Front Plant Sci"},{"key":"4554_CR28","doi-asserted-by":"publisher","first-page":"109150","DOI":"10.1016\/j.envint.2024.109150","volume":"194","author":"L Wang","year":"2024","unstructured":"Wang L, Wang S, Su H, Cai H, Song Y, Gong X, Zhang Y (2024) Multi-omics profiling reveals elevated CO2-enhanced tolerance of trifolium repens L. to lead stress through environment-plant-microbiome interactions. Environ Int 194:109150","journal-title":"Environ Int"},{"key":"4554_CR30","doi-asserted-by":"publisher","first-page":"122661","DOI":"10.1016\/j.jhazmat.2020.122661","volume":"395","author":"Y Wu","year":"2020","unstructured":"Wu Y, Ma L, Liu Q, Vesterg\u00e5rd M, Topalovic O, Wang Q, Feng Y (2020) The plant-growth promoting bacteria promote cadmium uptake by inducing a hormonal crosstalk and lateral root formation in a hyperaccumulator plant sedum Alfredii. J Hazard Mater 395:122661","journal-title":"J Hazard Mater"},{"key":"4554_CR31","doi-asserted-by":"publisher","first-page":"249","DOI":"10.17660\/ActaHortic.2008.767.26","volume":"767","author":"J Yang","year":"2008","unstructured":"Yang J, Liu L, Gong Y, He L, Li X, Chen L (2008) Cadmium uptake character and stress effect on the growth and antioxidant enzymes activities in Raphanus sativus. Acta Hort 767:249","journal-title":"Acta Hort"},{"key":"4554_CR32","doi-asserted-by":"publisher","first-page":"171497","DOI":"10.1016\/j.scitotenv.2024.171497","volume":"923","author":"X Yang","year":"2024","unstructured":"Yang X, Chen Y, Liu W, Huang T, Yang Y, Mao Y, Meng Y (2024) Combined transcriptomics and metabolomics to analyse the response of cuminum cyminum L. under Pb stress. Sci Total Environ 923:171497","journal-title":"Sci Total Environ"},{"key":"4554_CR33","doi-asserted-by":"publisher","first-page":"168615","DOI":"10.1016\/j.scitotenv.2023.168615","volume":"910","author":"Y Zhang","year":"2024","unstructured":"Zhang Y, Song Z, Zhao H, Chen H, Zhao B (2024) Integrative physiological, transcriptomic and metabolomic analysis reveals how the roots of two ornamental hydrangea macrophylla cultivars Cope with lead (Pb) toxicity. Sci Total Environ 910:168615","journal-title":"Sci Total Environ"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-025-04554-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-025-04554-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-025-04554-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T20:06:22Z","timestamp":1771272382000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00203-025-04554-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,14]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["4554"],"URL":"https:\/\/doi.org\/10.1007\/s00203-025-04554-2","relation":{},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,14]]},"assertion":[{"value":"28 August 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 October 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that there is no known conflict of interest associated with this publication and there has been no significant financial support received for this work. The authors would like to state that they take full responsibility of the entire data presented in the manuscript.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}}],"article-number":"18"}}