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Here, we describe the isolation and characterization of pTJK, one of the first bacteriophages reported to infect\n                    <jats:italic>Mammaliicoccus lentus<\/jats:italic>\n                    , recovered together with its host strain TJK24 from sewage-contaminated stream water. pTJK is a novel lytic phage with a broad host range, capable of infecting multiple\n                    <jats:italic>Mammaliicoccus<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    species. It significantly reduced biofilm formation and disrupted preformed biofilms, including in non-host strains, and displayed synergistic activity with gentamicin and erythromycin. The phage remained viable under moderate temperature and pH variations, UV exposure, and chloroform treatment. Genome analysis revealed a 135.8\u00a0kb double-stranded DNA genome with 231 ORFs, including multiple endolysins and depolymerases potentially involved in antibiofilm activity, and no detected resistance or host virulence genes. Comparative genomics placed pTJK within the genus\n                    <jats:italic>Sciuriunavirus<\/jats:italic>\n                    , representing a novel yet-to-be-defined species. These findings highlight the rarity, genomic distinctiveness, and biotechnological potential of pTJK, reinforcing the relevance of\n                    <jats:italic>Staphylococcaceae<\/jats:italic>\n                    phages as promising tools to combat antimicrobial resistance.\n                  <\/jats:p>","DOI":"10.1007\/s00203-026-04786-w","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T08:43:24Z","timestamp":1773132204000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["pTJK, a rare Mammaliicoccus lentus phage with broad-host-range, antibiofilm, and synergistic interactions with antimicrobials against resistant Staphylococcaceae"],"prefix":"10.1007","volume":"208","author":[{"given":"Faizan","family":"Ahmad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Rocha","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor Emanuel Lanes","family":"Viana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandy","family":"de Almada Estanislau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nohman","family":"Rasheed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7596-3619","authenticated-orcid":false,"given":"Rafael Reis","family":"de Rezende","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3526-0975","authenticated-orcid":false,"given":"Eduardo Lu\u00eds Menezes","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8203-5244","authenticated-orcid":false,"given":"Poliane Alfenas","family":"Zerbini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-4149","authenticated-orcid":false,"given":"Marisa Alves Nogueira","family":"Diaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8134-8337","authenticated-orcid":false,"given":"Monalessa F\u00e1bia","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6433-7521","authenticated-orcid":false,"given":"Marcia","family":"Giambiagi-deMarval","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3856-0882","authenticated-orcid":false,"given":"Ciro C\u00e9sar","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"4786_CR1","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1007\/s00284-025-04079-3","volume":"82","author":"F Ahmad","year":"2025","unstructured":"Ahmad F, Martuchelle SS, Andrade-Oliveira AL et al (2025a) From farm to community: dispersal of potentially pathogenic Staphylococcus and Mammaliicoccus species and antimicrobial resistance across shared environments. 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