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In\n                    <jats:italic>Caulobacter vibrioides<\/jats:italic>\n                    , the\n                    <jats:italic>cabp<\/jats:italic>\n                    gene encodes a previously uncharacterized calcium-binding protein (CaBP). In silico analysis predicted a 133-aa protein with three EF-hand motifs and an N-terminal Sec signal peptide. Consistently, CaBP exhibited a Ca\u00b2\u207a-dependent mobility shift and was localized to the periplasm in a SecA-dependent manner. Transcriptional analyses revealed a non-canonical organization in which the\n                    <jats:italic>sigV<\/jats:italic>\n                    promoter lies within the\n                    <jats:italic>cabp<\/jats:italic>\n                    coding sequence, although both genes are independently transcribed. Deletion of\n                    <jats:italic>cabp<\/jats:italic>\n                    increased biomass and stationary-phase survival. Expression analyses showed that SigmaV modulates\n                    <jats:italic>cabp<\/jats:italic>\n                    , while CaBP positively affects\n                    <jats:italic>sigV<\/jats:italic>\n                    expression, forming a Ca\u00b2\u207a and pH-responsive regulatory loop. Both genes were induced by acid stress and calcium limitation. The mutant displayed altered cell asymmetry and increased extracellular matrix production. These findings identify for the first time that CaBP as a periplasmic EF-hand calcium sensor linking ionic and pH signals to SigmaV-dependent stress regulation.\n                  <\/jats:p>","DOI":"10.1007\/s00203-026-04894-7","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:48:46Z","timestamp":1778654926000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A calcium-binding protein\u2013sigV regulatory circuit modulates stress adaptation and persistence in Caulobacter vibrioides"],"prefix":"10.1007","volume":"208","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1605-3978","authenticated-orcid":false,"given":"Elaine Luzia","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2999-1218","authenticated-orcid":false,"given":"Rita de C\u00e1ssia Garcia","family":"Sim\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"4894_CR1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12193398","volume":"12","author":"BC Agaras","year":"2023","unstructured":"Agaras BC, Grossi CEM, Ulloa RM (2023) Unveiling the secrets of calcium-dependent proteins in plant growth-promoting rhizobacteria: an abundance of discoveries awaits. 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