{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:38:38Z","timestamp":1793032718681,"version":"4.5.0"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"The Science and Technology Plan Projects at the Municipal Level of Shannan","award":["SNSBJKJJHXM2025008"],"award-info":[{"award-number":["SNSBJKJJHXM2025008"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Microbiol"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1007\/s00203-026-05120-0","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T06:31:49Z","timestamp":1786343509000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification, antimicrobial resistance, and biofilm-forming capability of bacterial isolates from bovine mastitis in the high-altitude region of Shannan, Tibet"],"prefix":"10.1007","volume":"208","author":[{"given":"Yunxia","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingqiong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihan","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawa","family":"Zhuoga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ouzhu","family":"Cuomu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changmin","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"5120_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2020.103885","volume":"226","author":"MF Addis","year":"2020","unstructured":"Addis MF, Maffioli EM, Ceciliani F et al (2020) Influence of subclinical mastitis and intramammary infection by coagulase-negative staphylococci on the cow milk peptidome. J Proteomics 226:103885","journal-title":"J Proteomics"},{"issue":"1","key":"5120_CR2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.23147","volume":"25","author":"SA Algharib","year":"2024","unstructured":"Algharib SA, Dawood AS, Huang L et al (2024) Basic concepts, recent advances, and future perspectives in the diagnosis of bovine mastitis. J Vet Sci 25(1):e18","journal-title":"J Vet Sci"},{"issue":"11","key":"5120_CR3","doi-asserted-by":"publisher","first-page":"e0071323","DOI":"10.1128\/MRA.00713-23","volume":"12","author":"TT Anika","year":"2023","unstructured":"Anika TT, Noman ZA, Islam MS et al (2023) Draft genome sequence of multidrug-resistant Escherichia coli MAHK_SCM_BAU_30A strain isolated from a subclinical mastitis cow in Bangladesh [J]. Microbiol Resour Announc 12(11):e0071323","journal-title":"Microbiol Resour Announc"},{"issue":"4","key":"5120_CR4","doi-asserted-by":"publisher","first-page":"e0007326","DOI":"10.1128\/mra.00073-26","volume":"15","author":"KY Arafat","year":"2026","unstructured":"Arafat KY et al (2026) Draft genome sequence and annotations of the mastitis-associated multidrug-resistant Staphylococcus aureus strain MBBL_MP1. Microbiol resource announcements vol 15(4):e0007326. https:\/\/doi.org\/10.1128\/mra.00073-26","journal-title":"Microbiol resource announcements vol"},{"key":"5120_CR5","first-page":"9","volume":"13","author":"N Belay","year":"2022","unstructured":"Belay N, Mohammed N, Seyoum W (2022) Bovine Mastitis: Prevalence, Risk Factors, and Bacterial Pathogens Isolated in Lactating Cows in Gamo Zone, Southern Ethiopia [J]. Vet Med (Auckl) 13:9\u201319","journal-title":"Vet Med (Auckl)"},{"issue":"5","key":"5120_CR6","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.3390\/microorganisms14051125","volume":"14","author":"CM Bouari","year":"2026","unstructured":"Bouari CM, Nad\u0103\u015f GC, Cr\u0103ciun S, Fi\u021b NI (2026) Staphylococcusaureus in Bovine Mastitis: Pathogenesis, Antimicrobial Resistance, and Emerging Control Strategies. Microorganisms 14(5):1125. https:\/\/doi.org\/10.3390\/microorganisms14051125 PMID: 42197509; PMCID: PMC13210330","journal-title":"Microorganisms"},{"key":"5120_CR7","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1167693","volume":"14","author":"ML Breser","year":"2023","unstructured":"Breser ML, Tisera L, Orellano MS et al (2023) Chitosan can improve antimicrobial treatment independently of bacterial lifestyle, biofilm biomass intensity and antibiotic resistance pattern in non-aureus staphylococci (NAS) isolated from bovine clinical mastitis. Front Microbiol 14:1167693","journal-title":"Front Microbiol"},{"key":"5120_CR8","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1185995","volume":"10","author":"S Chen","year":"1982","unstructured":"Chen S et al (1982) Prevalence of clinical mastitis and its associated risk factors among dairy cattle in mainland China during 1982\u20132022: a systematic review and meta-analysis. Front Vet Sci 10:1185995. https:\/\/doi.org\/10.3389\/fvets.2023.1185995","journal-title":"Front Vet Sci"},{"issue":"1","key":"5120_CR9","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-024-03415-0","volume":"24","author":"YA Getahun","year":"2024","unstructured":"Getahun YA, Abey SL, Beyene AM et al (2024) Coagulase-negative staphylococci from bovine milk: antibiogram profiles and virulent gene detection. BMC Microbiol 24(1):263","journal-title":"BMC Microbiol"},{"issue":"2","key":"5120_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics17020186","volume":"17","author":"CA Godoy","year":"2025","unstructured":"Godoy CA et al (2025) Antimicrobial and antibiofilm activity of chitosan nanoparticles against Staphylococcus aureus strains isolated from bovine mastitis milk. Pharmaceutics 17(2):186. https:\/\/doi.org\/10.3390\/pharmaceutics17020186","journal-title":"Pharmaceutics"},{"issue":"5","key":"5120_CR11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0268262","volume":"17","author":"G Han","year":"2022","unstructured":"Han G, Zhang B, Luo Z et al (2022) Molecular typing and prevalence of antibiotic resistance and virulence genes in Streptococcus agalactiae isolated from Chinese dairy cows with clinical mastitis. PLoS One 17(5):e0268262","journal-title":"PLoS One"},{"issue":"6","key":"5120_CR12","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens14060566","volume":"14","author":"E Kaczorek-\u0141ukowska","year":"2025","unstructured":"Kaczorek-\u0141ukowska E et al (2025) The effects of low concentrations of nisin on biofilm formation by Staphylococcus aureus isolated from dairy cattle. Pathogens (Basel) 14(6):566. https:\/\/doi.org\/10.3390\/pathogens14060566","journal-title":"Pathogens (Basel)"},{"key":"5120_CR13","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00772-26","author":"HJ Kang","year":"2026","unstructured":"Kang HJ et al (2026) Molecular and phenotypic characterization of Streptococcus uberis isolates from bovine mastitis milk in South Korea. Microbiol Spectr. https:\/\/doi.org\/10.1128\/spectrum.00772-26","journal-title":"Microbiol Spectr"},{"key":"5120_CR14","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2025.107851","volume":"207","author":"V Kumar","year":"2025","unstructured":"Kumar V, Nayakvadi S, Prakash K et al (2025) Isolation, antimicrobial resistance and biofilm gene analysis of MRSA in clinical and sub-clinical bovine mastitis. Microb Pathog 207:107851","journal-title":"Microb Pathog"},{"key":"5120_CR15","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8833","volume":"8","author":"W Li","year":"2020","unstructured":"Li W, Xue M, Yu L et al (2020) QseBC is involved in the biofilm formation and antibiotic resistance in Escherichia coli isolated from bovine mastitis. PeerJ 8:e8833","journal-title":"PeerJ"},{"key":"5120_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155358","volume":"125","author":"J Liu","year":"2024","unstructured":"Liu J, Gao Y, Zhang H et al (2024) Forsythiaside A attenuates mastitis via PINK1\/Parkin-mediated mitophagy. Phytomedicine 125:155358","journal-title":"Phytomedicine"},{"key":"5120_CR17","doi-asserted-by":"publisher","first-page":"107461","DOI":"10.1016\/j.micpath.2025.107461","volume":"203","author":"TT Mallick","year":"2025","unstructured":"Mallick TT, Rahman MM, Siddique N et al (2025) Molecular and genomic investigation unveils Pseudomonas putida as an emerging multidrug-resistant pathogen linked to bovine clinical mastitis [J]. Microb Pathog 203:107461","journal-title":"Microb Pathog"},{"issue":"1","key":"5120_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03501-3","volume":"18","author":"F Mohammadian","year":"2022","unstructured":"Mohammadian F, Rahmani HK, Bidarian B et al (2022) Isolation and evaluation of the efficacy of bacteriophages against multidrug-resistant (MDR), methicillin-resistant (MRSA) and biofilm-producing strains of Staphylococcus aureus recovered from bovine mastitis. BMC Vet Res 18(1):406","journal-title":"BMC Vet Res"},{"issue":"7","key":"5120_CR19","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1007\/s00284-023-03324-x","volume":"80","author":"I Raheel","year":"2023","unstructured":"Raheel I, Mohammed AN, Mohamed AA (2023) The efficacy of bacteriocins against biofilm-producing bacteria causing bovine clinical mastitis in dairy farms: a new strategy. Curr Microbiol 80(7):229","journal-title":"Curr Microbiol"},{"issue":"4","key":"5120_CR20","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001708","volume":"12","author":"KD Rich","year":"2026","unstructured":"Rich KD et al (2026) Phylogenomics and gene association analysis support probiotic potential in a subset of Staphylococcus xylosus isolates. Microb Genomics 12(4):001708. https:\/\/doi.org\/10.1099\/mgen.0.001708","journal-title":"Microb Genomics"},{"issue":"Pt 2","key":"5120_CR21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.126689","volume":"253","author":"YNV Sabino","year":"2023","unstructured":"Sabino YNV, Ara\u00fajo Domingues KC, Mathur H et al (2023) Exopolysaccharides produced by Bacillus spp. inhibit biofilm formation by Staphylococcus aureus strains associated with bovine mastitis. Int J Biol Macromol 253(Pt 2):126689","journal-title":"Int J Biol Macromol"},{"issue":"1","key":"5120_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/01652176.2024.2363626","volume":"44","author":"A Saleem","year":"2024","unstructured":"Saleem A, Saleem Bhat S, F A O et al (2024) Immunotherapy in mastitis: state of knowledge, research gaps and way forward [J]. Vet Q 44(1):1\u201323","journal-title":"Vet Q"},{"issue":"1","key":"5120_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-45643-w","volume":"13","author":"S Sokolov","year":"2023","unstructured":"Sokolov S, Brovko F, Solonin A et al (2023) Genomic analysis and assessment of pathogenic (toxicogenic) potential of Staphylococcus haemolyticus and Bacillus paranthracis consortia isolated from bovine mastitis in Russia. Sci Rep 13(1):18646","journal-title":"Sci Rep"},{"key":"5120_CR24","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12121159","author":"D Tomani\u0107","year":"2025","unstructured":"Tomani\u0107 D, Kladar N, Kova\u010devi\u0107 Z (2025) Antibiotic residues in milk as a consequence of mastitis treatment: balancing animal welfare and one health risks. Vet Sci. https:\/\/doi.org\/10.3390\/vetsci12121159","journal-title":"Vet Sci"},{"key":"5120_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2025.11.017","author":"Y Xu","year":"2025","unstructured":"Xu Y, Zhao J, Huang N et al (2025) Metagenomic characterization of the resistome, bacteriome and mobilome in raw milk from intensive farming systems. J Adv Res. https:\/\/doi.org\/10.1016\/j.jare.2025.11.017","journal-title":"J Adv Res"},{"key":"5120_CR26","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12111057","author":"C Yu","year":"2025","unstructured":"Yu C, Vivian KH, Fan S et al (2025) Bovine mastitis-derived Bacillus cereus in Inner Mongolia: strain characterization, virulence factor identification, and pathogenicity validation. Vet Sci. https:\/\/doi.org\/10.3390\/vetsci12111057","journal-title":"Vet Sci"}],"container-title":["Archives of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-026-05120-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-026-05120-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00203-026-05120-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:03:04Z","timestamp":1793030584000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00203-026-05120-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["5120"],"URL":"https:\/\/doi.org\/10.1007\/s00203-026-05120-0","relation":{},"ISSN":["0302-8933","1432-072X"],"issn-type":[{"value":"0302-8933","type":"print"},{"value":"1432-072X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]},"assertion":[{"value":"21 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 July 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 July 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 August 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study involved the collection and microbiological analysis of bovine milk samples from dairy cows on farms and smallholder households in Shannan, Tibet, China. No experimental infection, treatment intervention, invasive procedure, or animal sacrifice was performed. Milk samples were collected by trained veterinary personnel during routine mastitis investigation and herd health monitoring using standard aseptic procedures, with all reasonable measures taken to minimize animal stress and discomfort. Permission and informed consent for sample collection were obtained from the farm managers or animal owners before sampling. Because the study was non-interventional and used milk samples collected for bacteriological examination, formal approval by an animal ethics committee was not required. All procedures complied with relevant Chinese regulations and local veterinary practice requirements for the care and welfare of dairy cattle.","order":2,"name":"Ethics","label":"Research involving human and\/or animal participants","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"558"}}