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Human-induced pluripotent stem cells (hiPSC) are ideal for the development of such methods, because they are easy to retrieve by conversion of adult somatic cells and can differentiate into most cell types of the body. Advanced three-dimensional (3D) cultures of these cells, so-called embryoid bodies (EBs), moreover mimic the early developing embryo. We took advantage of this to develop a novel human toxicity assay to predict chemically induced developmental toxicity, which we termed the PluriBeat assay. We employed three different hiPSC lines from male and female donors and a robust microtiter plate-based method to produce EBs. We differentiated the cells into cardiomyocytes and introduced a scoring system for a quantitative readout of the assay\u2014cardiomyocyte contractions in the EBs observed on day 7. Finally, we tested the three compounds thalidomide (2.3\u201336\u00a0\u00b5M), valproic acid (25\u2013300\u00a0\u00b5M), and epoxiconazole (1.3\u201320\u00a0\u00b5M) on beating and size of the EBs. We were able to detect the human-specific teratogenicity of thalidomide and found the rodent toxicant epoxiconazole as more potent than thalidomide in our assay. We conclude that the PluriBeat assay is a novel method for predicting chemicals\u2019 adverse effects on embryonic development.<\/jats:p>","DOI":"10.1007\/s00204-020-02856-6","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T21:02:40Z","timestamp":1595451760000},"page":"3831-3846","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["A novel human pluripotent stem cell-based assay to predict developmental toxicity"],"prefix":"10.1007","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8016-7190","authenticated-orcid":false,"given":"Karin","family":"Lauschke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0588-607X","authenticated-orcid":false,"given":"Anna Kjerstine","family":"Rosenmai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Meiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Christiane","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikkel Aabech","family":"Rasmussen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4517-6881","authenticated-orcid":false,"given":"Bj\u00f8rn","family":"Holst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7985-2676","authenticated-orcid":false,"given":"Camilla","family":"Taxvig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny Katarina","family":"Emn\u00e9us","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6822-3572","authenticated-orcid":false,"given":"Anne Marie","family":"Vinggaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,22]]},"reference":[{"key":"2856_CR1","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.tiv.2007.07.013","volume":"22","author":"S Adler","year":"2008","unstructured":"Adler S, Pellizzer C, Hareng L et al (2008) First steps in establishing a developmental toxicity test method based on human embryonic stem cells. 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