{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T04:03:26Z","timestamp":1793333006608,"version":"4.5.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T00:00:00Z","timestamp":1571184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T00:00:00Z","timestamp":1571184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100010263","name":"Bingol University","doi-asserted-by":"crossref","award":["BAPSSHMYO. 2016.00.001"],"award-info":[{"award-number":["BAPSSHMYO. 2016.00.001"]}],"id":[{"id":"10.13039\/501100010263","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Naunyn-Schmiedeberg's Arch Pharmacol"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1007\/s00210-019-01741-z","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T19:40:29Z","timestamp":1571254829000},"page":"325-337","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":145,"title":["Protective effect of chrysin on cyclophosphamide-induced hepatotoxicity and nephrotoxicity via the inhibition of oxidative stress, inflammation, and apoptosis"],"prefix":"10.1007","volume":"393","author":[{"given":"Yusuf","family":"Temel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sefa","family":"Kucukler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serkan","family":"Y\u0131ld\u0131r\u0131m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-554X","authenticated-orcid":false,"given":"Cuneyt","family":"Caglayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih Mehmet","family":"Kandemir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,16]]},"reference":[{"key":"1741_CR1","doi-asserted-by":"crossref","unstructured":"Aebi H (1984) [13] Catalase in vitro. Methods Enzymol. Elsevier, pp 121-126","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"1741_CR2","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1007\/s00280-016-3152-1","volume":"78","author":"M Ahlmann","year":"2016","unstructured":"Ahlmann M, Hempel G (2016) The effect of cyclophosphamide on the immune system: implications for clinical cancer therapy. Cancer Chemother Pharmacol 78:661\u2013671","journal-title":"Cancer Chemother Pharmacol"},{"key":"1741_CR3","doi-asserted-by":"crossref","unstructured":"Alqahtani S, Mahmoud AM (2016, 2016) Gamma-glutamylcysteine ethyl ester protects against cyclophosphamide-induced liver injury and hematologic alterations via upregulation of PPAR\u03b3 and attenuation of oxidative stress, inflammation, and apoptosis. Oxidative Med Cell Longev","DOI":"10.1155\/2016\/4016209"},{"key":"1741_CR4","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1080\/13813455.2017.1422766","volume":"124","author":"F Benzer","year":"2018","unstructured":"Benzer F, Kandemir FM, Kucukler S, Comakl\u0131 S, Caglayan C (2018) Chemoprotective effects of curcumin on doxorubicin-induced nephrotoxicity in wistar rats: by modulating inflammatory cytokines, apoptosis, oxidative stress and oxidative DNA damage. Arch Physiol Biochem 124:448\u2013457","journal-title":"Arch Physiol Biochem"},{"key":"1741_CR5","doi-asserted-by":"crossref","unstructured":"Caglayan C (2019) The effects of naringin on different cyclophosphamide-induced organ toxicities in rats: investigation of changes in some metabolic enzyme activities. Environ Sci Pollut Res 1-10","DOI":"10.1007\/s11356-019-05915-3"},{"key":"1741_CR6","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.biopha.2018.03.119","volume":"102","author":"C Caglayan","year":"2018","unstructured":"Caglayan C, Kandemir FM, Y\u0131ld\u0131r\u0131m S, Kucukler S, K\u0131l\u0131nc MA, Saglam YS (2018a) Zingerone ameliorates cisplatin-induced ovarian and uterine toxicity via suppression of sex hormone imbalances, oxidative stress, inflammation and apoptosis in female wistar rats. Biomed Pharmacother 102:517\u2013530","journal-title":"Biomed Pharmacother"},{"key":"1741_CR7","doi-asserted-by":"publisher","first-page":"20968","DOI":"10.1007\/s11356-018-2242-5","volume":"25","author":"C Caglayan","year":"2018","unstructured":"Caglayan C, Temel Y, Kandemir FM, Yildirim S, Kucukler S (2018b) Naringin protects against cyclophosphamide-induced hepatotoxicity and nephrotoxicity through modulation of oxidative stress, inflammation, apoptosis, autophagy, and DNA damage. Environ Sci Pollut Res 25:20968\u201320984","journal-title":"Environ Sci Pollut Res"},{"key":"1741_CR8","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.jtemb.2019.07.011","volume":"56","author":"C Caglayan","year":"2019","unstructured":"Caglayan C, Kandemir FM, Darendelio\u011flu E, Y\u0131ld\u0131r\u0131m S, Kucukler S, Dortbudak MB (2019a) Rutin ameliorates mercuric chloride-induced hepatotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. J Trace Elem Med Biol 56:60\u201368","journal-title":"J Trace Elem Med Biol"},{"key":"1741_CR9","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.jtemb.2019.04.007","volume":"54","author":"C Caglayan","year":"2019","unstructured":"Caglayan C, Kandemir FM, Yildirim S, Kucukler S, Eser G (2019b) Rutin protects mercuric chloride-induced nephrotoxicity via targeting of aquaporin 1 level, oxidative stress, apoptosis and inflammation in rats. J Trace Elem Med Biol 54:69\u201378","journal-title":"J Trace Elem Med Biol"},{"key":"1741_CR10","doi-asserted-by":"publisher","first-page":"100","DOI":"10.3892\/mmr.2012.1036","volume":"6","author":"Q Du","year":"2012","unstructured":"Du Q, Gu X, Cai J, Huang M, Su M (2012) Chrysin attenuates allergic airway inflammation by modulating the transcription factors T-bet and GATA-3 in mice. Mol Med Rep 6:100\u2013104","journal-title":"Mol Med Rep"},{"key":"1741_CR11","doi-asserted-by":"publisher","first-page":"e21960","DOI":"10.1002\/jbt.21960","volume":"31","author":"E Eldutar","year":"2017","unstructured":"Eldutar E, Kandemir FM, Kucukler S, Caglayan C (2017) Restorative effects of Chrysin pretreatment on oxidant\u2013antioxidant status, inflammatory cytokine production, and apoptotic and autophagic markers in acute paracetamol-induced hepatotoxicity in rats: an experimental and biochemical study. J Biochem Mol Toxicol 31:e21960","journal-title":"J Biochem Mol Toxicol"},{"key":"1741_CR12","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.biopha.2017.05.103","volume":"92","author":"AA El-Kholy","year":"2017","unstructured":"El-Kholy AA, Elkablawy MA, El-Agamy DS (2017) Lutein mitigates cyclophosphamide induced lung and liver injury via NF-\u03baB\/MAPK dependent mechanism. Biomed Pharmacother 92:519\u2013527","journal-title":"Biomed Pharmacother"},{"key":"1741_CR13","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1038\/nrclinonc.2009.146","volume":"6","author":"A Emadi","year":"2009","unstructured":"Emadi A, Jones RJ, Brodsky RA (2009) Cyclophosphamide and cancer: golden anniversary. Nat Rev Clin Oncol 6:638","journal-title":"Nat Rev Clin Oncol"},{"key":"1741_CR14","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.taap.2015.01.012","volume":"283","author":"AA Ganai","year":"2015","unstructured":"Ganai AA, Khan AA, Malik ZA, Farooqi H (2015) Genistein modulates the expression of NF-\u03baB and MAPK (p-38 and ERK1\/2), thereby attenuating d-Galactosamine induced fulminant hepatic failure in Wistar rats. Toxicol Appl Pharmacol 283:139\u2013146","journal-title":"Toxicol Appl Pharmacol"},{"key":"1741_CR15","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/s00204-011-0774-2","volume":"86","author":"I G\u00fclcin","year":"2012","unstructured":"G\u00fclcin I (2012) Antioxidant activity of food constituents: an overview. Arch Toxicol 86:345\u2013391","journal-title":"Arch Toxicol"},{"key":"1741_CR16","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3109\/13880209.2014.908399","volume":"53","author":"E Habibi","year":"2015","unstructured":"Habibi E, Shokrzadeh M, Chabra A, Naghshvar F, Keshavarz-Maleki R, Ahmadi A (2015) Protective effects of Origanum vulgare ethanol extract against cyclophosphamide-induced liver toxicity in mice. Pharm Biol 53:10\u201315","journal-title":"Pharm Biol"},{"key":"1741_CR17","doi-asserted-by":"publisher","first-page":"2109","DOI":"10.1007\/s12272-011-1214-9","volume":"34","author":"JB Jeong","year":"2011","unstructured":"Jeong JB, Hong SC, Jeong HJ, Koo JS (2011) Anti-inflammatory effect of 2-methoxy-4-vinylphenol via the suppression of NF-\u03baB and MAPK activation, and acetylation of histone H3. Arch Pharm Res 34:2109\u20132116","journal-title":"Arch Pharm Res"},{"key":"1741_CR18","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3390\/scipharm85010004","volume":"85","author":"F Kandemir","year":"2017","unstructured":"Kandemir F, Kucukler S, Eldutar E, Caglayan C, G\u00fcl\u00e7in I (2017a) Chrysin protects rat kidney from paracetamol-induced oxidative stress, inflammation, apoptosis, and autophagy: a multi-biomarker approach. Sci Pharm 85:4","journal-title":"Sci Pharm"},{"key":"1741_CR19","doi-asserted-by":"publisher","first-page":"e12398","DOI":"10.1111\/jfbc.12398","volume":"41","author":"FM Kandemir","year":"2017","unstructured":"Kandemir FM, Kucukler S, Caglayan C, Gur C, Batil AA, G\u00fcl\u00e7in \u0130 (2017b) Therapeutic effects of silymarin and naringin on methotrexate-induced nephrotoxicity in rats: biochemical evaluation of anti-inflammatory, antiapoptotic, and antiautophagic properties. J Food Biochem 41:e12398","journal-title":"J Food Biochem"},{"key":"1741_CR20","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1016\/j.biopha.2018.06.048","volume":"105","author":"FM Kandemir","year":"2018","unstructured":"Kandemir FM, Yildirim S, Kucukler S, Caglayan C, Mahamadu A, Dortbudak MB (2018) Therapeutic efficacy of zingerone against vancomycin-induced oxidative stress, inflammation, apoptosis and aquaporin 1 permeability in rat kidney. Biomed Pharmacother 105:981\u2013991","journal-title":"Biomed Pharmacother"},{"key":"1741_CR21","doi-asserted-by":"crossref","unstructured":"Kandemir FM, Yildirim S, Caglayan C, Kucukler S, Eser G (2019) Protective effects of zingerone on cisplatin-induced nephrotoxicity in female rats. Environ Sci Pollut Res 1-13","DOI":"10.1007\/s11356-019-05505-3"},{"key":"1741_CR22","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.lfs.2012.01.012","volume":"90","author":"A Kumari","year":"2012","unstructured":"Kumari A, Kakkar P (2012) Lupeol prevents acetaminophen-induced in vivo hepatotoxicity by altering the Bax\/Bcl-2 and oxidative stress-mediated mitochondrial signaling cascade. Life Sci 90:561\u2013570","journal-title":"Life Sci"},{"key":"1741_CR23","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1016\/j.biopha.2018.06.161","volume":"106","author":"M Kuzu","year":"2018","unstructured":"Kuzu M, Kandemir FM, Yildirim S, Kucukler S, Caglayan C, Turk E (2018) Morin attenuates doxorubicin-induced heart and brain damage by reducing oxidative stress, inflammation and apoptosis. Biomed Pharmacother 106:443\u2013453","journal-title":"Biomed Pharmacother"},{"key":"1741_CR24","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.cbi.2019.05.017","volume":"308","author":"M Kuzu","year":"2019","unstructured":"Kuzu M, Y\u0131ld\u0131r\u0131m S, Kandemir FM, K\u00fc\u00e7\u00fckler S, \u00c7a\u011flayan C, T\u00fcrk E, D\u00f6rtbudak MB (2019) Protective effect of morin on doxorubicin-induced hepatorenal toxicity in rats. Chem Biol Interact 308:89\u2013100","journal-title":"Chem Biol Interact"},{"key":"1741_CR25","first-page":"952","volume":"71","author":"RA Lawrence","year":"1976","unstructured":"Lawrence RA, Burk RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. BBRC 71:952\u2013958","journal-title":"BBRC"},{"key":"1741_CR26","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.3390\/ijms18071412","volume":"18","author":"S-R Lin","year":"2017","unstructured":"Lin S-R, Fu Y-S, Tsai M-J, Cheng H, Weng C-F (2017) Natural compounds from herbs that can potentially execute as autophagy inducers for cancer therapy. Int J Mol Sci 18:1412","journal-title":"Int J Mol Sci"},{"key":"1741_CR27","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1016\/j.biopha.2016.10.097","volume":"84","author":"Q Liu","year":"2016","unstructured":"Liu Q, Lin X, Li H, Yuan J, Peng Y, Dong L, Dai S (2016) Paeoniflorin ameliorates renal function in cyclophosphamide-induced mice via AMPK suppressed inflammation and apoptosis. Biomed Pharmacother 84:1899\u20131905","journal-title":"Biomed Pharmacother"},{"key":"1741_CR28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"OH Lowry","year":"1951","unstructured":"Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265\u2013275","journal-title":"J Biol Chem"},{"key":"1741_CR29","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1016\/j.apjtm.2017.08.005","volume":"10","author":"P Mahipal","year":"2017","unstructured":"Mahipal P, Pawar RS (2017) Nephroprotective effect of Murraya koenigii on cyclophosphamide induced nephrotoxicity in rats. Asian Pac J Trop Med 10:808\u2013812","journal-title":"Asian Pac J Trop Med"},{"key":"1741_CR30","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.biopha.2016.12.047","volume":"86","author":"AM Mahmoud","year":"2017","unstructured":"Mahmoud AM, Germoush MO, Alotaibi MF, Hussein OE (2017) Possible involvement of Nrf2 and PPAR\u03b3 up-regulation in the protective effect of umbelliferone against cyclophosphamide-induced hepatotoxicity. Biomed Pharmacother 86:297\u2013306","journal-title":"Biomed Pharmacother"},{"key":"1741_CR31","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.ejphar.2014.01.065","volume":"728","author":"EM Mantawy","year":"2014","unstructured":"Mantawy EM, El-Bakly WM, Esmat A, Badr AM, El-Demerdash E (2014) Chrysin alleviates acute doxorubicin cardiotoxicity in rats via suppression of oxidative stress, inflammation and apoptosis. Eur J Pharmacol 728:107\u2013118","journal-title":"Eur J Pharmacol"},{"key":"1741_CR32","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.cell.2010.01.028","volume":"140","author":"N Mizushima","year":"2010","unstructured":"Mizushima N, Yoshimori T, Levine B (2010) Methods in mammalian autophagy research. Cell 140:313\u2013326","journal-title":"Cell"},{"key":"1741_CR33","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.cbi.2015.02.021","volume":"231","author":"S Nafees","year":"2015","unstructured":"Nafees S, Rashid S, Ali N, Hasan SK, Sultana S (2015) Rutin ameliorates cyclophosphamide induced oxidative stress and inflammation in Wistar rats: role of NF\u03baB\/MAPK pathway. Chem Biol Interact 231:98\u2013107","journal-title":"Chem Biol Interact"},{"key":"1741_CR34","doi-asserted-by":"crossref","unstructured":"Oyagbemi AA, Omobowale OT, Asenuga ER, Akinleye AS, Ogunsanwo RO, Saba AB (2016) Cyclophosphamide-induced hepatotoxicity in wistar rats: the modulatory role of gallic acid as a hepatoprotective and chemopreventive phytochemical. Int J Prev Med:7","DOI":"10.4103\/2008-7802.177898"},{"key":"1741_CR35","first-page":"359","volume":"16","author":"ZA Placer","year":"1966","unstructured":"Placer ZA, Cushman LL, Johnson BC (1966) Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. AnBio 16:359\u2013364","journal-title":"AnBio"},{"key":"1741_CR36","first-page":"192","volume":"25","author":"J Sedlak","year":"1968","unstructured":"Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman\u2019s reagent. AnBio 25:192\u2013205","journal-title":"AnBio"},{"key":"1741_CR37","first-page":"1392. e1391","volume":"80","author":"O Sinanoglu","year":"2012","unstructured":"Sinanoglu O, Yener AN, Ekici S, Midi A, Aksungar FB (2012) The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats. Urology 80:1392. e1391\u20131392. e1396","journal-title":"Urology"},{"key":"1741_CR38","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1016\/j.biopha.2018.08.039","volume":"107","author":"C Singh","year":"2018","unstructured":"Singh C, Prakash C, Tiwari KN, Mishra SK, Kumar V (2018) Premna integrifolia ameliorates cyclophosphamide-induced hepatotoxicity by modulation of oxidative stress and apoptosis. Biomed Pharmacother 107:634\u2013643","journal-title":"Biomed Pharmacother"},{"key":"1741_CR39","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1093\/clinchem\/34.3.497","volume":"34","author":"Y Sun","year":"1988","unstructured":"Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497\u2013500","journal-title":"Clin Chem"},{"key":"1741_CR40","doi-asserted-by":"publisher","first-page":"e21995","DOI":"10.1002\/jbt.21995","volume":"31","author":"P Taslimi","year":"2017","unstructured":"Taslimi P, Caglayan C, Gulcin \u0130 (2017) The impact of some natural phenolic compounds on carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and \u03b1-glycosidase enzymes: an antidiabetic, anticholinergic, and antiepileptic study. J Biochem Mol Toxicol 31:e21995","journal-title":"J Biochem Mol Toxicol"},{"key":"1741_CR41","doi-asserted-by":"crossref","unstructured":"Taslimi P, Kandemir FM, Demir Y, \u0130lerit\u00fcrk M, Temel Y, Caglayan C, Gul\u00e7in \u0130 (2019) The antidiabetic and anticholinergic effects of chrysin on cyclophosphamide-induced multiple organ toxicity in rats: pharmacological evaluation of some metabolic enzyme activities. J Biochem Mol Toxicol:e22313","DOI":"10.1002\/jbt.22313"},{"key":"1741_CR42","first-page":"93","volume":"499","author":"X Zhai","year":"2018","unstructured":"Zhai X, Zhang Z, Liu W, Liu B, Zhang R, Wang W, Zheng W, Xu F, Wang J, Chen Y (2018) Protective effect of ALDH2 against cyclophosphamide-induced acute hepatotoxicity via attenuating oxidative stress and reactive aldehydes. BBRC 499:93\u201398","journal-title":"BBRC"}],"container-title":["Naunyn-Schmiedeberg's Archives of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00210-019-01741-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00210-019-01741-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00210-019-01741-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T01:03:30Z","timestamp":1611536610000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00210-019-01741-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,16]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["1741"],"URL":"https:\/\/doi.org\/10.1007\/s00210-019-01741-z","relation":{},"ISSN":["0028-1298","1432-1912"],"issn-type":[{"value":"0028-1298","type":"print"},{"value":"1432-1912","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,16]]},"assertion":[{"value":"30 July 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 September 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 October 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study was designed and conducted according to ethical norms approved by the Local Animal Experimentation Ethics Committee of the Bingol University (Bingol, Turkey) (approval no. 2016-4\/5).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}