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Bleomycin (BLM) is an antibiotic that was first described as a chemotherapeutic agent. 3,3\u2032-methylenebis(1-ethyl-4-hydroxyquinolin-2(1<jats:italic>H<\/jats:italic>)-one) was reported to have anti-inflammatory, anti-apoptotic, and anti-oxidative properties. The current work aimed to assess the possible protective effects and the mechanism of protection of 3,3\u2032-methylenebis-(1-ethyl-4-hydroxyquinolin-2(1<jats:italic>H<\/jats:italic>)-one) on BLM-induced lung injury in addition to the effect and underlying mechanisms of nuclear factor-erythroid-related factor 2 pathway against this injury. Rats were equally divided into four groups: control group, BLM group, 1-ethyl-4-hydroxyquinolin-2(1<jats:italic>H<\/jats:italic>)-one-treated group, and BLM with 1-ethyl-4-hydroxyquinolin-2(1<jats:italic>H<\/jats:italic>)-one-treated group. At the end of the work, the blood samples were proceeded for biochemical study. Lung specimens were obtained for biochemical, histological, and immunohistochemical study. The results exhibited a significant increase in both malondialdehyde and tumor necrotic factor-\u03b1 with a significant decrease in glutathione, superoxide dismutase, IL 10, surfactant protein A, and nuclear factor erythroid 2-related factor 2 in BLM group. The lung histological results showed various morphological changes in the form of disturbed architecture, inflammatory cell infiltration, and intraluminal debris. This group also displayed a significant increase in the mean surface area fraction of anti-cleaved caspase 3, while group IV exhibited amelioration in the previously mentioned parameters and histological alternations that were induced by BLM. It could be concluded that 3,3\u2032-methylenebis(1-ethyl-4-hydroxyquinolin-2(1<jats:italic>H<\/jats:italic>)-one) has anti-oxidative, anti-inflammatory, and anti-apoptotic protective effects against BLM-induced lung injury.<\/jats:p>","DOI":"10.1007\/s00210-022-02324-1","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T14:24:00Z","timestamp":1670509440000},"page":"771-788","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Potential protective effect of 3,3\u2032-methylenebis(1-ethyl-4-hydroxyquinolin-2(1H)-one) against bleomycin-induced lung injury in male albino rat via modulation of Nrf2 pathway: biochemical, histological, and immunohistochemical study"],"prefix":"10.1007","volume":"396","author":[{"given":"Sara Mohamed Naguib Abdel","family":"Hafez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Entesar Ali","family":"Saber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neven Makram","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maha Yehia","family":"Kamel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashraf A.","family":"Aly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El-Shimaa M. 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