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In our study, we investigated the ameliorative effect of tannic acid (TA), a polyphenol, against DOX-induced testicular and reproductive toxicity in rats. For this purpose, rats divided into four groups (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095 each). Rats were treated with DOX cumulatively 30\u00a0mg\/kg (5\u00a0mg\/kg by six equal injections) for 2\u00a0weeks to induce testicular toxicity. Rats were then treated daily with TA (50\u00a0mg\/kg) and DOX\u2009+\u2009TA combination. After treatments, animals were decapitated and testicular tissues were removed. Then, to examine the effect of DOX and TA treatments on oxidative stress, changes in\n                    <jats:italic>Sod<\/jats:italic>\n                    ,\n                    <jats:italic>Cat<\/jats:italic>\n                    ,\n                    <jats:italic>Gpx<\/jats:italic>\n                    ,\n                    <jats:italic>Gst<\/jats:italic>\n                    , and\n                    <jats:italic>Gr<\/jats:italic>\n                    specific activities and mRNA levels were determined. To determine the effects on inflammation, changes in the expression levels of\n                    <jats:italic>Tnf-\u03b1<\/jats:italic>\n                    ,\n                    <jats:italic>IL6<\/jats:italic>\n                    ,\n                    <jats:italic>Foxo1<\/jats:italic>\n                    ,\n                    <jats:italic>Foxo3<\/jats:italic>\n                    ,\n                    <jats:italic>Cox2<\/jats:italic>\n                    , and\n                    <jats:italic>Inos<\/jats:italic>\n                    genes were examined. In order to examine the therapeutic effect of TA on the spermatogenesis process, mRNA levels of\n                    <jats:italic>Dazl<\/jats:italic>\n                    ,\n                    <jats:italic>Amh<\/jats:italic>\n                    , and\n                    <jats:italic>Ddx4<\/jats:italic>\n                    , which regulate reproductive functions, were determined. Additionally, changes in oxidative damage markers GSH, MDA, 8-OHdG, iNOS, and TNF-a and changes in mitochondrial DNA copy number were also investigated. The results showed that DOX treatment caused a decrease in the levels of oxidative stress and reproductive parameters and an increase in inflammation and DNA damage parameters. However, it was determined that oxidative stress, inflammation, and tissue damage decreased in testicular tissue after TA treatment. In addition, it was observed that TA also improved the expression levels of reproductive genes. When all the data were evaluated together, it was determined that TA administration has a therapeutic effect against the damage and toxicity caused by DOX.\n                  <\/jats:p>","DOI":"10.1007\/s00210-025-04238-0","type":"journal-article","created":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T05:27:40Z","timestamp":1746854860000},"page":"15587-15598","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Investigation of the effect of tannic acid on doxorubicin-\u0131nduced testicular damage and functions in a rat model"],"prefix":"10.1007","volume":"398","author":[{"given":"Emine","family":"Toraman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Ceylan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,10]]},"reference":[{"key":"4238_CR1","doi-asserted-by":"publisher","unstructured":"Abd\u2010Elrazek AM, El\u2010dash HA, Said NI (2020) The role of propolis against paclitaxel\u2010induced oligospermia, sperm abnormality, oxidative stress and DNA damage in testes of male rats. 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