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Dexpanthenol (DEX) is known for its antioxidant, anti-inflammatory, and anti-apoptotic properties. This study aimed to investigate the potential protective effects of dexpanthenol in a rat model of diatrizoate-induced CIN. In this study, 32 Wistar albino rats were randomly divided into four groups: control, Urografin (URO; 10\u00a0mL\/kg, intraperitoneal [i.p.]), URO\u2009+\u2009DEX (500\u00a0mg\/kg, i.p. for 3\u00a0days), and DEX alone. Renal function markers (serum urea and creatinine), total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured. Histopathological evaluation and immunohistochemical analysis of tumor necrosis factor-alpha (TNF-\u03b1) and caspase-3 (Cas-3) were performed. Additionally, SIRT1, Bcl-2, Bax, and p53 mRNA expression levels were assessed. URO administration increased TOS and OSI values and caused significant renal histopathological damage. TNF-\u03b1 and Cas-3 immunoreactivity, along with Bax and p53 gene expression, were significantly elevated, while Bcl-2 and SIRT1 expression was suppressed. DEX treatment provided partial improvement in these changes, contributed to the preservation of renal architecture, and was associated with improvements in biochemical and molecular parameters approaching those of the control levels. In conclusion, DEX may exert nephroprotective effects against CIN by reducing oxidative stress, inhibiting pro-inflammatory and apoptotic pathways, and increasing anti-apoptotic gene expression. These findings provide a preclinical basis supporting the idea that dexpanthenol is a promising candidate for further translational and clinical studies in contrast-induced renal injury.<\/jats:p>","DOI":"10.1007\/s00210-026-05520-5","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T03:22:59Z","timestamp":1780888979000},"page":"18261-18271","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective effects of dexpanthenol against diatrizoate-induced contrast nephropathy via the SIRT1\u2013p53\u2013Bcl-2\/Bax pathway in rats"],"prefix":"10.1007","volume":"399","author":[{"given":"Cenk Mustafa","family":"Havabulut","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demet","family":"G\u00fcnd\u00fcz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emine","family":"Sarman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130lter","family":"\u0130lhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammet Yusuf","family":"Tepeba\u015f\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdurrahman","family":"G\u00fclal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehtap","family":"Savran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halil","family":"A\u015fc\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"issue":"1","key":"5520_CR1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-019-2745-x","volume":"37","author":"A Al Shoyaib","year":"2020","unstructured":"Al Shoyaib A, Archie SR, Karamyan VT (2020) Intraperitoneal route of drug administration: should it be used in experimental animal studies? 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All experimental procedures involving animals were conducted in accordance with institutional regulations and internationally accepted guidelines for laboratory animal care, including the NIH Guide (8th edition). The study was designed and reported in accordance with the ARRIVE 2.0 recommendations.","order":1,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no known competing financial or non-financial interests that could have appeared to influence the work reported in this paper.","order":2,"name":"Ethics","label":"Conflict of \u0131nterest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"During the preparation of this manuscript, the authors used ChatGPT (OpenAI) to improve language clarity and readability. After using this tool, the authors reviewed and edited the content as necessary and take full responsibility for the final content of the publication.","order":3,"name":"Ethics","label":"Declaration of generative AI use","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":4,"name":"Ethics","label":"Clinical trial number","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}