{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:51:09Z","timestamp":1791582669174,"version":"4.3.4"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Freie Universit\u00e4t Berlin","id":[{"id":"https:\/\/ror.org\/046ak2485","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Naunyn-Schmiedeberg's Arch Pharmacol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Skin sensitization testing is moving towards human-relevant, non-animal approaches, yet many current in vitro methods address only individual key events of the adverse outcome pathway. Here, a reconstructed immunocompetent human skin model is presented, generated entirely from induced pluripotent stem cell-derived fibroblasts, keratinocytes, and dendritic cells. The model reproduced key structural features of human skin and enabled the combined assessment of keratinocyte activation and dendritic-cell maturation and migration following topical exposure to skin sensitizers. By integrating epidermal and immune responses within a single three-dimensional skin construct, this model provides a mechanistically informative platform for skin sensitization research and supports the development of ethically responsible testing strategies.<\/jats:p>","DOI":"10.1007\/s00210-026-05652-8","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T07:23:33Z","timestamp":1783322613000},"page":"20035-20038","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Young Investigator Award 2026: Development of a reconstructed induced pluripotent stem cell-derived immunocompetent skin model"],"prefix":"10.1007","volume":"399","author":[{"given":"Marla","family":"Dubau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"issue":"2","key":"5652_CR1","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1111\/j.1600-0536.2008.01444.x","volume":"60","author":"DA Basketter","year":"2009","unstructured":"Basketter DA, McFadden JF, Gerberick F, Cockshott A, Kimber I (2009) Nothing is perfect, not even the local lymph node assay: a commentary and the implications for REACH. Contact Dermat 60(2):65\u201369. https:\/\/doi.org\/10.1111\/j.1600-0536.2008.01444.x","journal-title":"Contact Dermat"},{"key":"5652_CR2","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5082\/5\/3\/035011","author":"DYS Chau","year":"2013","unstructured":"Chau DYS, Johnson C, Macneil S, Haycock JW, Ghaemmaghami AM (2013) The development of a 3D immunocompetent model of human skin. Biofabrication. https:\/\/doi.org\/10.1088\/1758-5082\/5\/3\/035011","journal-title":"Biofabrication"},{"key":"5652_CR3","doi-asserted-by":"publisher","DOI":"10.1177\/20417314251336296","author":"M Dubau","year":"2025","unstructured":"Dubau M, Tripetchr T, Mahmoud L, Schumacher F, Kleuser B (2025) Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances. J Tissue Eng. https:\/\/doi.org\/10.1177\/20417314251336296","journal-title":"J Tissue Eng"},{"key":"5652_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2020.104713","author":"SN Kolle","year":"2020","unstructured":"Kolle SN, Landsiedel R, Natsch A (2020) Replacing the refinement for skin sensitization testing: considerations to the implementation of adverse outcome pathway (AOP)-based defined approaches (DA) in OECD guidelines. Regul Toxicol Pharmacol. https:\/\/doi.org\/10.1016\/j.yrtph.2020.104713","journal-title":"Regul Toxicol Pharmacol"},{"key":"5652_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2023.105333","author":"A Natsch","year":"2023","unstructured":"Natsch A, Kleinstreuer N, Asturiol D (2023) Reduced specificity for the local lymph node assay for lipophilic chemicals: implications for the validation of new approach methods for skin sensitization. Regul Toxicol Pharmacol. https:\/\/doi.org\/10.1016\/j.yrtph.2023.105333","journal-title":"Regul Toxicol Pharmacol"},{"key":"5652_CR6","doi-asserted-by":"publisher","unstructured":"OECD (2010) Test Guideline No. 429: Skin Sensitisation: Local Lymph Node Assay. OECD Guidelines for the Testing of Chemicals, Section 4. OECD Publishing, Paris. https:\/\/doi.org\/10.1787\/9789264071100-en","DOI":"10.1787\/9789264071100-en"},{"key":"5652_CR7","unstructured":"OECD (2012) The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins. Part 1: Scientific Evidence. OECD Series on Testing and Assessment, No. 168. ENV\/JM\/MONO(2012)10\/PART1. OECD Publishing, Paris."},{"key":"5652_CR8","doi-asserted-by":"publisher","unstructured":"OECD (2022) Test No. 406: Skin Sensitisation: Guinea Pig Maximisation Test and Buehler Test. OECD Guidelines for the Testing of Chemicals, Section 4. OECD Publishing, Paris. https:\/\/doi.org\/10.1787\/9789264070660-en. Accessed 29 Jun 2026","DOI":"10.1787\/9789264070660-en"},{"key":"5652_CR9","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.tiv.2019.02.002","volume":"57","author":"MT Schellenberger","year":"2019","unstructured":"Schellenberger MT, Bock U, Hennen J, Groeber-Becker F, Walles H, Bl\u00f6meke B (2019) A coculture system composed of THP-1 cells and 3D reconstructed human epidermis to assess activation of dendritic cells by sensitizing chemicals after topical exposure. Toxicol In Vitro 57:62\u201366. https:\/\/doi.org\/10.1016\/j.tiv.2019.02.002","journal-title":"Toxicol In Vitro"},{"key":"5652_CR10","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.tiv.2019.05.008","volume":"60","author":"F Tourneix","year":"2019","unstructured":"Tourneix F, Al\u00e9p\u00e9e N, Detroyer A, Eilstein J, Martinozzi Teissier S, Nardelli L, No\u00e7airi H, Pauloin T, Piroird C, Del Bufalo A (2019) Assessment of a defined approach based on a stacking prediction model to identify skin sensitization hazard. Toxicol In Vitro 60:134\u2013143. https:\/\/doi.org\/10.1016\/j.tiv.2019.05.008","journal-title":"Toxicol In Vitro"}],"container-title":["Naunyn-Schmiedeberg's Archives of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00210-026-05652-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00210-026-05652-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00210-026-05652-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:03:19Z","timestamp":1791579799000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00210-026-05652-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,6]]},"references-count":10,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["5652"],"URL":"https:\/\/doi.org\/10.1007\/s00210-026-05652-8","relation":{},"ISSN":["0028-1298","1432-1912"],"issn-type":[{"value":"0028-1298","type":"print"},{"value":"1432-1912","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,6]]},"assertion":[{"value":"23 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}