{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T03:02:26Z","timestamp":1767927746371,"version":"3.49.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T00:00:00Z","timestamp":1423699200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1007\/s00216-015-8509-5","type":"journal-article","created":{"date-parts":[[2015,2,11]],"date-time":"2015-02-11T09:36:57Z","timestamp":1423647417000},"page":"2847-2858","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["High-sensitivity MALDI-TOF MS quantification of anthrax lethal toxin for diagnostics and evaluation of medical countermeasures"],"prefix":"10.1007","volume":"407","author":[{"given":"Anne E.","family":"Boyer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maribel","family":"Gallegos-Candela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conrad P.","family":"Quinn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian R.","family":"Woolfitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith O.","family":"Brumlow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Isbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato C.","family":"Lins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,2,12]]},"reference":[{"key":"8509_CR1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.biochem.75.103004.142728","volume":"76","author":"JA Young","year":"2007","unstructured":"Young JA, Collier RJ (2007) Anthrax toxin: receptor binding, internalization, pore formation, and translocation. Annu Rev Biochem 76:243\u2013265","journal-title":"Annu Rev Biochem"},{"issue":"6","key":"8509_CR2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1128\/CVI.00023-06","volume":"13","author":"R Mabry","year":"2006","unstructured":"Mabry R, Brasky K, Geiger R, Carrion R Jr, Hubbard GB, Leppla S, Patterson JL, Georgiou G, Iverson BL (2006) Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays. Clin Vaccine Immunol 13(6):671\u2013677","journal-title":"Clin Vaccine Immunol"},{"issue":"3","key":"8509_CR3","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1128\/CVI.00412-08","volume":"16","author":"S Tang","year":"2009","unstructured":"Tang S, Moayeri M, Chen Z, Harma H, Zhao J, Hu H, Purcell RH, Leppla SH, Hewlett IK (2009) Detection of anthrax toxin by an ultrasensitive immunoassay using europium nanoparticles. Clin Vaccine Immunol 16(3):408\u2013413","journal-title":"Clin Vaccine Immunol"},{"issue":"3","key":"8509_CR4","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1007\/s10895-014-1354-7","volume":"24","author":"N Cohen","year":"2014","unstructured":"Cohen N, Mechaly A, Mazor O, Fisher M, Zahavy E (2014) Rapid homogenous time-resolved fluorescence (HTRF) immunoassay for anthrax detection. J Fluoresc 24(3):795\u2013801","journal-title":"J Fluoresc"},{"key":"8509_CR5","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jim.2014.05.008","volume":"408","author":"RA Stoddard","year":"2014","unstructured":"Stoddard RA, Quinn CP, Schiffer JM, Boyer AE, Goldstein J, Bagarozzi DA, Soroka SD, Dauphin LA, Hoffmaster AR (2014) Detection of anthrax protective antigen (PA) using europium labeled anti-PA monoclonal antibody and time-resolved fluorescence. J Immunol Methods 408:78\u201388","journal-title":"J Immunol Methods"},{"issue":"10","key":"8509_CR6","doi-asserted-by":"crossref","first-page":"9452","DOI":"10.1021\/nn404296k","volume":"7","author":"B Farrow","year":"2013","unstructured":"Farrow B, Hong SA, Romero EC, Lai B, Coppock MB, Deyle KM, Finch AS, Stratis-Cullum DN, Agnew HD, Yang S, Heath JR (2013) A chemically synthesized capture agent enables the selective, sensitive, and robust electrochemical detection of anthrax protective antigen. ACS Nano 7(10):9452\u20139460","journal-title":"ACS Nano"},{"issue":"1","key":"8509_CR7","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ab.2012.02.040","volume":"425","author":"AI Dragan","year":"2012","unstructured":"Dragan AI, Albrecht MT, Pavlovic R, Keane-Myers AM, Geddes CD (2012) Ultra-fast pg\/ml anthrax toxin (protective antigen) detection assay based on microwave-accelerated metal-enhanced fluorescence. Anal Biochem 425(1):54\u201361","journal-title":"Anal Biochem"},{"issue":"12","key":"8509_CR8","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1007\/s00216-013-6752-1","volume":"405","author":"A Mechaly","year":"2013","unstructured":"Mechaly A, Cohen N, Weiss S, Zahavy E (2013) A novel homogeneous immunoassay for anthrax detection based on the AlphaLISA method: detection of B. anthracis spores and protective antigen (PA) in complex samples. Anal Bioanal Chem 405(12):3965\u20133972","journal-title":"Anal Bioanal Chem"},{"issue":"1","key":"8509_CR9","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.diagmicrobio.2013.05.006","volume":"77","author":"N Ghosh","year":"2013","unstructured":"Ghosh N, Gupta N, Gupta G, Boopathi M, Pal V, Goel AK (2013) Detection of protective antigen, an anthrax specific toxin in human serum by using surface plasmon resonance. Diagn Microbiol Infect Dis 77(1):14\u201319","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"30","key":"8509_CR10","doi-asserted-by":"crossref","first-page":"12424","DOI":"10.1073\/pnas.0905409106","volume":"106","author":"S Liu","year":"2009","unstructured":"Liu S, Crown D, Miller-Randolph S, Moayeri M, Wang H, Hu H, Morley T, Leppla SH (2009) Capillary morphogenesis protein-2 is the major receptor mediating lethality of anthrax toxin in vivo. Proc Natl Acad Sci U S A 106(30):12424\u201312429","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"8509_CR11","doi-asserted-by":"crossref","first-page":"5227","DOI":"10.1074\/jbc.M210321200","volume":"278","author":"S Liu","year":"2003","unstructured":"Liu S, Leppla SH (2003) Cell surface tumor endothelium marker 8 cytoplasmic tail-independent anthrax toxin binding, proteolytic processing, oligomer formation, and internalization. J Biol Chem 278(7):5227\u20135234","journal-title":"J Biol Chem"},{"issue":"6619","key":"8509_CR12","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1038\/385833a0","volume":"385","author":"C Petosa","year":"1997","unstructured":"Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC (1997) Crystal structure of the anthrax toxin protective antigen. Nature 385(6619):833\u2013838","journal-title":"Nature"},{"issue":"3","key":"8509_CR13","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.jmb.2009.07.037","volume":"392","author":"AF Kintzer","year":"2009","unstructured":"Kintzer AF, Thoren KL, Sterling HJ, Dong KC, Feld GK, Tang II, Zhang TT, Williams ER, Berger JM, Krantz BA (2009) The protective antigen component of anthrax toxin forms functional octameric complexes. J Mol Biol 392(3):614\u2013629","journal-title":"J Mol Biol"},{"issue":"3","key":"8509_CR14","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1021\/bi015860m","volume":"41","author":"J Mogridge","year":"2002","unstructured":"Mogridge J, Cunningham K, Collier RJ (2002) Stoichiometry of anthrax toxin complexes. Biochemistry 41(3):1079\u20131082","journal-title":"Biochemistry"},{"issue":"3","key":"8509_CR15","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1083\/jcb.200211018","volume":"160","author":"L Abrami","year":"2003","unstructured":"Abrami L, Liu S, Cosson P, Leppla SH, van der Goot FG (2003) Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process. J Cell Biol 160(3):321\u2013328","journal-title":"J Cell Biol"},{"issue":"10","key":"8509_CR16","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1073\/pnas.79.10.3162","volume":"79","author":"SH Leppla","year":"1982","unstructured":"Leppla SH (1982) Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells. Proc Natl Acad Sci U S A 79(10):3162\u20133166","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5364","key":"8509_CR17","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.280.5364.734","volume":"280","author":"NS Duesbery","year":"1998","unstructured":"Duesbery NS, Webb CP, Leppla SH, Gordon VM, Klimpel KR, Copeland TD, Ahn NG, Oskarsson MK, Fukasawa K, Paull KD, Vande Woude GF (1998) Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 280(5364):734\u2013737","journal-title":"Science"},{"issue":"3","key":"8509_CR18","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1099\/00221287-138-3-543","volume":"138","author":"JW Ezzell Jr","year":"1992","unstructured":"Ezzell JW Jr, Abshire TG (1992) Serum protease cleavage of Bacillus anthracis protective antigen. J Gen Microbiol 138(3):543\u2013549","journal-title":"J Gen Microbiol"},{"issue":"11","key":"8509_CR19","doi-asserted-by":"crossref","first-page":"5175","DOI":"10.1128\/IAI.00719-07","volume":"75","author":"M Moayeri","year":"2007","unstructured":"Moayeri M, Wiggins JF, Leppla SH (2007) Anthrax protective antigen cleavage and clearance from the blood of mice and rats. Infect Immun 75(11):5175\u20135184","journal-title":"Infect Immun"},{"issue":"11","key":"8509_CR20","doi-asserted-by":"crossref","first-page":"10834","DOI":"10.1074\/jbc.M412210200","volume":"280","author":"RG Panchal","year":"2005","unstructured":"Panchal RG, Halverson KM, Ribot W, Lane D, Kenny T, Abshire TG, Ezzell JW, Hoover TA, Powell B, Little S, Kasianowicz JJ, Bavari S (2005) Purified Bacillus anthracis lethal toxin complex formed in vitro and during infection exhibits functional and biological activity. J Biol Chem 280(11):10834\u201310839","journal-title":"J Biol Chem"},{"issue":"22","key":"8509_CR21","doi-asserted-by":"crossref","first-page":"8463","DOI":"10.1021\/ac701741s","volume":"79","author":"AE Boyer","year":"2007","unstructured":"Boyer AE, Quinn CP, Woolfitt AR, Pirkle JL, McWilliams LG, Stamey KL, Bagarozzi DA, Hart JC Jr, Barr JR (2007) Detection and quantification of anthrax lethal factor in serum by mass spectrometry. Anal Chem 79(22):8463\u20138470","journal-title":"Anal Chem"},{"issue":"3","key":"8509_CR22","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.3390\/molecules16032391","volume":"16","author":"AE Boyer","year":"2011","unstructured":"Boyer AE, Gallegos-Candela M, Lins RC, Kuklenyik Z, Woolfitt A, Moura H, Kalb S, Quinn CP, Barr JR (2011) Quantitative mass spectrometry for bacterial protein toxins\u2014a sensitive, specific, high-throughput tool for detection and diagnosis. Molecules 16(3):2391\u20132413","journal-title":"Molecules"},{"key":"8509_CR23","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1021\/ac1030144","volume":"83","author":"Z Kuklenyik","year":"2011","unstructured":"Kuklenyik Z, Boyer AE, Lins R, Quinn CP, Gallegos-Candela M, Woolfitt A, Pirkle JL, Barr JR (2011) Comparison of MALDI-TOF-MS and HPLC-ESI-MS\/MS for endopeptidase activity-based quantification of anthrax lethal factor in serum. Anal Chem 83:1760\u20131765","journal-title":"Anal Chem"},{"key":"8509_CR24","unstructured":"Gallegos-Candela M, Boyer AE, Quinn CP, Woolfitt AR, Barr JR (2009) Validation of rapid, sensitive, quantitative MALDI-TOF MS assays for anthrax lethal factor and lethal toxin. In: Bacillus-ACT 2009: The International Bacillus anthracis, B. cereus, and B. thuringiensis conference, Santa Fe, NM"},{"issue":"8","key":"8509_CR25","doi-asserted-by":"crossref","first-page":"3432","DOI":"10.1128\/IAI.00346-09","volume":"77","author":"AE Boyer","year":"2009","unstructured":"Boyer AE, Quinn CP, Hoffmaster AR, Kozel TR, Saile E, Marston CK, Percival A, Plikaytis BD, Woolfitt AR, Gallegos M, Sabourin P, McWilliams LG, Pirkle JL, Barr JR (2009) Kinetics of lethal factor and poly-d-glutamic acid antigenemia during inhalation anthrax in rhesus macaques. Infect Immun 77(8):3432\u20133441","journal-title":"Infect Immun"},{"issue":"7","key":"8509_CR26","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1086\/512372","volume":"44","author":"JJ Walsh","year":"2007","unstructured":"Walsh JJ, Pesik N, Quinn CP, Urdaneta V, Dykewicz CA, Boyer AE, Guarner J, Wilkins P, Norville KJ, Barr JR, Zaki SR, Patel JB, Reagan SP, Pirkle JL, Treadwell TA, Messonnier NR, Rotz LD, Meyer RF, Stephens DS (2007) A case of naturally acquired inhalation anthrax: clinical care and analyses of anti-protective antigen immunoglobulin G and lethal factor. Clin Infect Dis 44(7):968\u2013971","journal-title":"Clin Infect Dis"},{"issue":"2","key":"8509_CR27","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3201\/eid2002.130245","volume":"20","author":"MD Sprenkle","year":"2014","unstructured":"Sprenkle MD, Griffith J, Marinelli W, Boyer AE, Quinn CP, Pesik NT, Hoffmaster A, Keenan J, Juni BA, Blaney DD (2014) Lethal factor and anti-protective antigen IgG levels associated with inhalation anthrax, Minnesota, USA. Emerg Infect Dis 20(2):310\u2013314","journal-title":"Emerg Infect Dis"},{"issue":"11","key":"8509_CR28","doi-asserted-by":"crossref","first-page":"5684","DOI":"10.1128\/AAC.00458-13","volume":"57","author":"N Mytle","year":"2013","unstructured":"Mytle N, Hopkins RJ, Malkevich NV, Basu S, Meister GT, Sanford DC, Comer JE, Van Zandt KE, Al-Ibrahim M, Kramer WG, Howard C, Daczkowski N, Chakrabarti AC, Ionin B, Nabors GS, Skiadopoulos MH (2013) Evaluation of intravenous anthrax immune globulin for treatment of inhalation anthrax. Antimicrob Agents Chemother 57(11):5684\u20135692","journal-title":"Antimicrob Agents Chemother"},{"key":"8509_CR29","unstructured":"HHS-FDA, CDER (2001) Guidance for industry: bioanalytical method validation. Rockville, MD"},{"issue":"Suppl 1","key":"8509_CR30","first-page":"S49","volume":"29","author":"DA Armbruster","year":"2008","unstructured":"Armbruster DA, Pry T (2008) Limit of blank, limit of detection and limit of quantitation. Clin Biochem Rev Aust Assoc Clin Biochem 29(Suppl 1):S49\u2013S52","journal-title":"Clin Biochem Rev Aust Assoc Clin Biochem"},{"key":"8509_CR31","doi-asserted-by":"crossref","unstructured":"Anaraki S, Addiman S, Nixon G, Krahe D, Ghosh R, Brooks T, Lloyd G, Spencer R, Walsh A, McCloskey B, Lightfoot N (2008) Investigations and control measures following a case of inhalation anthrax in East London in a drum maker and drummer, October 2008. Euro Surveill 13(51)","DOI":"10.2807\/ese.13.51.19076-en"},{"issue":"3","key":"8509_CR32","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1074\/jbc.M511164200","volume":"281","author":"RA Melnyk","year":"2006","unstructured":"Melnyk RA, Hewitt KM, Lacy DB, Lin HC, Gessner CR, Li S, Woods VL Jr, Collier RJ (2006) Structural determinants for the binding of anthrax lethal factor to oligomeric protective antigen. J Biol Chem 281(3):1630\u20131635","journal-title":"J Biol Chem"},{"key":"8509_CR33","volume-title":"Quality assurance of chemical measurements","author":"JK Taylor","year":"1987","unstructured":"Taylor JK (1987) Quality assurance of chemical measurements. CRC Press LLC, United States"},{"key":"8509_CR34","unstructured":"Boyer AE, Quinn CP, Hoffmaster AR, Kozel T, Gallegos M, Saile E, Marston C, Beesley C, Percival A, Lins R, Woolfitt A, Pirkle JL, Barr JR (2010) Association of early lethal factor levels with survival during inhalation anthrax in rhesus macaques. In: ASM Biodefense and Emerging Diseases Research Meeting, Baltimore, MD. American Society for Microbiology"},{"issue":"13","key":"8509_CR35","doi-asserted-by":"crossref","first-page":"4830","DOI":"10.1073\/pnas.0500508102","volume":"102","author":"C Kim","year":"2005","unstructured":"Kim C, Gajendran N, Mittrucker HW, Weiwad M, Song YH, Hurwitz R, Wilmanns M, Fischer G, Kaufmann SH (2005) Human alpha-defensins neutralize anthrax lethal toxin and protect against its fatal consequences. Proc Natl Acad Sci U S A 102(13):4830\u20134835","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"8509_CR36","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1128\/IAI.00764-08","volume":"77","author":"JW Ezzell","year":"2009","unstructured":"Ezzell JW, Abshire TG, Panchal R, Chabot D, Bavari S, Leffel EK, Purcell B, Friedlander AM, Ribot WJ (2009) Association of Bacillus anthracis capsule with lethal toxin during experimental infection. Infect Immun 77(2):749\u2013755","journal-title":"Infect Immun"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00216-015-8509-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-015-8509-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-015-8509-5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T08:20:23Z","timestamp":1651220423000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00216-015-8509-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,12]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["8509"],"URL":"https:\/\/doi.org\/10.1007\/s00216-015-8509-5","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,12]]}}}