{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T21:40:00Z","timestamp":1788126000795,"version":"build-2803163510"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2015,12,16]],"date-time":"2015-12-16T00:00:00Z","timestamp":1450224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,12,16]],"date-time":"2015-12-16T00:00:00Z","timestamp":1450224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["NIH grant no. 1DP1DA034787-01"],"award-info":[{"award-number":["NIH grant no. 1DP1DA034787-01"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2016,2]]},"DOI":"10.1007\/s00216-015-9223-z","type":"journal-article","created":{"date-parts":[[2015,12,16]],"date-time":"2015-12-16T10:12:37Z","timestamp":1450260757000},"page":"1191-1204","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A simple nonradioactive method for the determination of the binding affinities of antibodies induced by hapten bioconjugates for drugs of abuse"],"prefix":"10.1007","volume":"408","author":[{"given":"Oscar B.","family":"Torres","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua F. G.","family":"Antoline","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi","family":"Jalah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur E.","family":"Jacobson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenner C.","family":"Rice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl R.","family":"Alving","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary R.","family":"Matyas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,12,16]]},"reference":[{"key":"9223_CR1","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1517\/14712598.2013.791278","volume":"13","author":"TR Kosten","year":"2013","unstructured":"Kosten TR, Domingo CB (2013) Can you vaccinate against substance abuse? Expert Opin Biol Ther 13:1093\u20131097","journal-title":"Expert Opin Biol Ther"},{"key":"9223_CR2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nri3130","volume":"12","author":"KD Janda","year":"2012","unstructured":"Janda KD, Treweek JB (2012) Vaccines targeting drugs of abuse: is the glass half-empty or half-full? Nat Rev Immunol 12:67\u201372","journal-title":"Nat Rev Immunol"},{"key":"9223_CR3","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1111\/j.1742-7843.2006.pto_233.x","volume":"98","author":"EJ Rook","year":"2006","unstructured":"Rook EJ, van Ree JM, van den Brink W, Hillebrand MJ, Huitema AD, Hendriks VM, Beijnen JH (2006) Pharmacokinetics and pharmacodynamics of high doses of pharmaceutically prepared heroin, by intravenous or by inhalation route in opioid-dependent patients. Basic Clin Pharmacol Toxicol 98:86\u201396","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"9223_CR4","doi-asserted-by":"publisher","first-page":"865","DOI":"10.2174\/187152711799219316","volume":"10","author":"GN Stowe","year":"2011","unstructured":"Stowe GN, Schlosburg JE, Vendruscolo LF, Edwards S, Misra KK, Schulteis G, Zakhari JS, Koob GF, Janda KD (2011) Developing a vaccine against multiple psychoactive targets: a case study of heroin. CNS Neurol Disord: Drug Targets 10:865\u2013875","journal-title":"CNS Neurol Disord: Drug Targets"},{"key":"9223_CR5","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1124\/jpet.113.212035","volume":"349","author":"IL Bogen","year":"2014","unstructured":"Bogen IL, Boix F, Nerem E, Morland J, Andersen JM (2014) A monoclonal antibody specific for 6-monoacetylmorphine reduces acute heroin effects in mice. J Pharmacol Exp Ther 349:568\u2013576","journal-title":"J Pharmacol Exp Ther"},{"key":"9223_CR6","doi-asserted-by":"publisher","first-page":"5221","DOI":"10.1021\/jm2004943","volume":"54","author":"FI Carroll","year":"2011","unstructured":"Carroll FI, Blough BE, Pidaparthi RR, Abraham P, Gong PK, Deng L, Huang X, Gunnell M, Lay JO Jr, Peterson EC, Owens SM (2011) Synthesis of mercapto-(+)-methamphetamine haptens and their use for obtaining improved epitope density on (+)-methamphetamine conjugate vaccines. J Med Chem 54:5221\u20135228","journal-title":"J Med Chem"},{"key":"9223_CR7","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1021\/acs.bioconjchem.5b00085","volume":"26","author":"R Jalah","year":"2015","unstructured":"Jalah R, Torres OB, Mayorov AV, Li F, Antoline JF, Jacobson AE, Rice KC, Deschamps JR, Beck Z, Alving CR, Matyas GR (2015) Efficacy, but not antibody titer or affinity, of a heroin hapten conjugate vaccine correlates with increasing hapten densities on tetanus toxoid, but not on CRM197 carriers. Bioconjug Chem 26:1041\u20131053","journal-title":"Bioconjug Chem"},{"key":"9223_CR8","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1016\/0019-2791(71)90454-X","volume":"8","author":"E Engvall","year":"1971","unstructured":"Engvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871\u2013874","journal-title":"Immunochemistry"},{"key":"9223_CR9","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/0014-5793(71)80319-8","volume":"15","author":"BK Van Weemen","year":"1971","unstructured":"Van Weemen BK, Schuurs AH (1971) Immunoassay using antigen-enzyme conjugates. FEBS Lett 15:232\u2013236","journal-title":"FEBS Lett"},{"key":"9223_CR10","doi-asserted-by":"crossref","first-page":"129","DOI":"10.4049\/jimmunol.109.1.129","volume":"109","author":"E Engvall","year":"1972","unstructured":"Engvall E, Perlmann P (1972) Enzyme-linked immunosorbent assay, ELISA. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol 109:129\u2013135","journal-title":"J Immunol"},{"key":"9223_CR11","first-page":"243","volume":"71","author":"JS Plested","year":"2003","unstructured":"Plested JS, Coull PA, Gidney MA (2003) ELISA. Methods Mol Med 71:243\u2013261","journal-title":"Methods Mol Med"},{"key":"9223_CR12","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/0022-1759(88)90279-7","volume":"106","author":"ME Devey","year":"1988","unstructured":"Devey ME, Bleasdale K, Lee S, Rath S (1988) Determination of the functional affinity of IgG1 and IgG4 antibodies to tetanus toxoid by isotype-specific solid-phase assays. J Immunol Methods 106:119\u2013125","journal-title":"J Immunol Methods"},{"key":"9223_CR13","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/0022-1759(88)90204-9","volume":"106","author":"S Rath","year":"1988","unstructured":"Rath S, Stanley CM, Steward MW (1988) An inhibition enzyme immunoassay for estimating relative antibody affinity and affinity heterogeneity. J Immunol Methods 106:245\u2013249","journal-title":"J Immunol Methods"},{"key":"9223_CR14","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1021\/mp400631w","volume":"11","author":"PT Bremer","year":"2014","unstructured":"Bremer PT, Schlosburg JE, Lively JM, Janda KD (2014) Injection route and TLR9 agonist addition significantly impact heroin vaccine efficacy. Mol Pharmaceutics 11:1075\u20131080","journal-title":"Mol Pharmaceutics"},{"key":"9223_CR15","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/j.bcp.2011.11.004","volume":"83","author":"M Pravetoni","year":"2012","unstructured":"Pravetoni M, Keyler DE, Pidaparthi RR, Carroll FI, Runyon SP, Murtaugh MP, Earley CA, Pentel PR (2012) Structurally distinct nicotine immunogens elicit antibodies with non-overlapping specificities. Biochem Pharmacol 83:543\u2013550","journal-title":"Biochem Pharmacol"},{"key":"9223_CR16","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/S0022-3565(25)25802-5","volume":"129","author":"EL Way","year":"1960","unstructured":"Way EL, Kemp JW, Young JM, Grassetti DR (1960) The pharmacologic effects of heroin in relationship to its rate of biotransformation. J Pharmacol Exp Ther 129:144\u2013154","journal-title":"J Pharmacol Exp Ther"},{"key":"9223_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0022-3565(25)32246-9","volume":"215","author":"O Lockridge","year":"1980","unstructured":"Lockridge O, Mottershaw-Jackson N, Eckerson HW, La Du BN (1980) Hydrolysis of diacetylmorphine (heroin) by human serum cholinesterase. J Pharmacol Exp Ther 215:1\u20138","journal-title":"J Pharmacol Exp Ther"},{"key":"9223_CR18","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1139\/y83-132","volume":"61","author":"JA Owen","year":"1983","unstructured":"Owen JA, Nakatsu K (1983) Diacetylmorphine (heroin) hydrolases in human blood. Can J Physiol Pharmacol 61:870\u2013875","journal-title":"Can J Physiol Pharmacol"},{"key":"9223_CR19","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1046\/j.1440-1681.1999.03090.x","volume":"26","author":"AY Salmon","year":"1999","unstructured":"Salmon AY, Goren Z, Avissar Y, Soreq H (1999) Human erythrocyte but not brain acetylcholinesterase hydrolyses heroin to morphine. Clin Exp Pharmacol Physiol 26:596\u2013600","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"9223_CR20","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1042\/bst0310620","volume":"31","author":"MR Redinbo","year":"2003","unstructured":"Redinbo MR, Bencharit S, Potter PM (2003) Human carboxylesterase 1: from drug metabolism to drug discovery. Biochem Soc Trans 31:620\u2013624","journal-title":"Biochem Soc Trans"},{"key":"9223_CR21","doi-asserted-by":"publisher","first-page":"5927","DOI":"10.1007\/s00216-014-8035-x","volume":"406","author":"OB Torres","year":"2014","unstructured":"Torres OB, Jalah R, Rice KC, Li F, Antoline JF, Iyer MR, Jacobson AE, Boutaghou MN, Alving CR, Matyas GR (2014) Characterization and optimization of heroin hapten-BSA conjugates: method development for the synthesis of reproducible hapten-based vaccines. Anal Bioanal Chem 406:5927\u20135937","journal-title":"Anal Bioanal Chem"},{"key":"9223_CR22","doi-asserted-by":"publisher","DOI":"10.1002\/9780470583128","volume-title":"Assay development: fundamentals and practices","author":"G Wu","year":"2010","unstructured":"Wu G (2010) Assay development: fundamentals and practices. Wiley, Hoboken"},{"key":"9223_CR23","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1016\/0076-6879(83)92046-3","volume":"92","author":"R M\u00fcller","year":"1983","unstructured":"M\u00fcller R (1983) Determination of affinity and specificity of anti-hapten antibodies by competitive radioimmunoassay. Methods Enzymol 92:589\u2013601","journal-title":"Methods Enzymol"},{"key":"9223_CR24","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1002\/(SICI)1098-2787(1999)18:2<119::AID-MAS2>3.0.CO;2-Z","volume":"18","author":"S Pichini","year":"1999","unstructured":"Pichini S, Altieri I, Pellegrini M, Zuccaro P, Pacifici R (1999) The role of liquid chromatography-mass spectrometry in the determination of heroin and related opioids in biological fluids. Mass Spectrom Rev 18:119\u2013130","journal-title":"Mass Spectrom Rev"},{"key":"9223_CR25","doi-asserted-by":"publisher","first-page":"7211","DOI":"10.1039\/C4OB01053A","volume":"12","author":"F Li","year":"2014","unstructured":"Li F, Cheng K, Antoline JF, Iyer MR, Matyas GR, Torres OB, Jalah R, Beck Z, Alving CR, Parrish DA, Deschamps JR, Jacobson AE, Rice KC (2014) Synthesis and immunological effects of heroin vaccines. Org Biomol Chem 12:7211\u20137232","journal-title":"Org Biomol Chem"},{"key":"9223_CR26","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1007\/BF01901562","volume":"13","author":"PR Droupadi","year":"1994","unstructured":"Droupadi PR, Meyers EA, Linthicum DS (1994) Spectroscopic evidence for charge-transfer complexation in monoclonal antibodies that bind opiates. J Protein Chem 13:297\u2013306","journal-title":"J Protein Chem"},{"key":"9223_CR27","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.vascn.2012.04.009","volume":"66","author":"A Gottas","year":"2012","unstructured":"Gottas A, Oiestad EL, Boix F, Ripel A, Thaulow CH, Pettersen BS, Vindenes V, Morland J (2012) Simultaneous measurement of heroin and its metabolites in brain extracellular fluid by microdialysis and ultra performance liquid chromatography tandem mass spectrometry. J Pharmacol Toxicol Methods 66:14\u201321","journal-title":"J Pharmacol Toxicol Methods"},{"key":"9223_CR28","doi-asserted-by":"publisher","first-page":"8149","DOI":"10.1021\/bi00192a020","volume":"33","author":"ML Fahnestock","year":"1994","unstructured":"Fahnestock ML, Johnson JL, Feldman RM, Tsomides TJ, Mayer J, Narhi LO, Bjorkman PJ (1994) Effects of peptide length and composition on binding to an empty class I MHC heterodimer. Biochemistry 33:8149\u20138158","journal-title":"Biochemistry"},{"key":"9223_CR29","doi-asserted-by":"publisher","first-page":"2804","DOI":"10.1016\/j.vaccine.2013.04.027","volume":"31","author":"GR Matyas","year":"2013","unstructured":"Matyas GR, Mayorov AV, Rice KC, Jacobson AE, Cheng K, Iyer MR, Li F, Beck Z, Janda KD, Alving CR (2013) Liposomes containing monophosphoryl lipid A: a potent adjuvant system for inducing antibodies to heroin hapten analogs. Vaccine 31:2804\u20132810","journal-title":"Vaccine"},{"key":"9223_CR30","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1016\/j.vaccine.2014.01.028","volume":"32","author":"GR Matyas","year":"2014","unstructured":"Matyas GR, Rice KC, Cheng K, Li F, Antoline JF, Iyer MR, Jacobson AE, Mayorov AV, Beck Z, Torres OB, Alving CR (2014) Facial recognition of heroin vaccine opiates: type 1 cross-reactivities of antibodies induced by hydrolytically stable haptenic surrogates of heroin, 6-acetylmorphine, and morphine. Vaccine 32:1473\u20131479","journal-title":"Vaccine"},{"key":"9223_CR31","doi-asserted-by":"publisher","first-page":"2840","DOI":"10.1055\/s-0031-1291151","volume":"44","author":"R Csuk","year":"2012","unstructured":"Csuk R, Vasileva G, Barthel A (2012) Towards an efficient preparation of hydromorphone. Synthesis 44:2840\u20132842","journal-title":"Synthesis"},{"key":"9223_CR32","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1039\/p19780001076","volume":"1","author":"S Uemura","year":"1978","unstructured":"Uemura S, Toshimitsu A, Okano M (1978) Nitration of aromatic hydrocarbons and ipso-nitrosodemetallation of arylmetal compounds in sodium nitrite\u2013trifluoroacetic acid. J Chem Soc Perkin Trans 1:1076\u20131079","journal-title":"J Chem Soc Perkin Trans"},{"key":"9223_CR33","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1002\/anie.201006867","volume":"50","author":"M Galibert","year":"2011","unstructured":"Galibert M, Renaudet O, Dumy P, Boturyn D (2011) Access to biomolecular assemblies through one-pot triple orthogonal chemoselective ligations. Angew Chem Int Ed Engl 50:1901\u20131904","journal-title":"Angew Chem Int Ed Engl"},{"key":"9223_CR34","unstructured":"Information on anti-morphine antibody (ab1060). http:\/\/www.abcam.com\/morphine-antibody-ab1060.html . Accessed on 23 June 2015"},{"key":"9223_CR35","doi-asserted-by":"publisher","first-page":"5382","DOI":"10.1016\/j.vaccine.2014.07.085","volume":"32","author":"CR Alving","year":"2014","unstructured":"Alving CR, Matyas GR, Torres O, Jalah R, Beck Z (2014) Adjuvants for vaccines to drugs of abuse and addiction. Vaccine 32:5382\u20135389","journal-title":"Vaccine"},{"key":"9223_CR36","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1139\/y78-105","volume":"56","author":"PT Smith","year":"1978","unstructured":"Smith PT, Hirst M, Gowdey CW (1978) Spontaneous hydrolysis of heroin in buffered solution. Can J Physiol Pharmacol 56:665\u2013667","journal-title":"Can J Physiol Pharmacol"},{"key":"9223_CR37","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1084\/jem.61.5.643","volume":"61","author":"K Landsteiner","year":"1935","unstructured":"Landsteiner K, Jacobs J (1935) Studies on the sensitization of animals with simple chemical compounds. J Exp Med 61:643\u2013656","journal-title":"J Exp Med"},{"key":"9223_CR38","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1084\/jem.64.4.625","volume":"64","author":"K Landsteiner","year":"1936","unstructured":"Landsteiner K, Jacobs J (1936) Studies on the sensitization of animals with simple chemical compounds II. J Exp Med 64:625\u2013639","journal-title":"J Exp Med"},{"key":"9223_CR39","doi-asserted-by":"publisher","first-page":"5195","DOI":"10.1021\/jm200461m","volume":"54","author":"GN Stowe","year":"2011","unstructured":"Stowe GN, Vendruscolo LF, Edwards S, Schlosburg JE, Misra KK, Schulteis G, Mayorov AV, Zakhari JS, Koob GF, Janda KD (2011) A vaccine strategy that induces protective immunity against heroin. J Med Chem 54:5195\u20135204","journal-title":"J Med Chem"},{"key":"9223_CR40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076557","volume":"8","author":"DC Pryde","year":"2013","unstructured":"Pryde DC, Jones LH, Gervais DP, Stead DR, Blakemore DC, Selby MD, Brown AD, Coe JW, Badland M, Beal DM, Glen R, Wharton Y, Miller GJ, White P, Zhang N, Benoit M, Robertson K, Merson JR, Davis HL, McCluskie MJ (2013) Selection of a novel anti-nicotine vaccine: influence of antigen design on antibody function in mice. PLoS One 8, e76557","journal-title":"PLoS One"},{"key":"9223_CR41","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1126\/science.168.3937.1347","volume":"168","author":"S Spector","year":"1970","unstructured":"Spector S, Parker CW (1970) Morphine: radioimmunoassay. Science 168:1347\u20131348","journal-title":"Science"},{"key":"9223_CR42","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1254\/jjp.24.707","volume":"24","author":"M Koida","year":"1974","unstructured":"Koida M, Takahashi M, Kaneto H (1974) The morphine 3-glucuronide directed antibody: its immunological specificity and possible use for radioimmunoassay of morphine in urine. Jpn J Pharmacol 24:707\u2013714","journal-title":"Jpn J Pharmacol"},{"key":"9223_CR43","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1038\/bjc.1990.363","volume":"62","author":"PJ Hoskin","year":"1990","unstructured":"Hoskin PJ, Hanks GW (1990) Morphine: pharmacokinetics and clinical practice. Br J Cancer 62:705\u2013707","journal-title":"Br J Cancer"},{"key":"9223_CR44","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.drudis.2011.12.018","volume":"17","author":"F Zhang","year":"2012","unstructured":"Zhang F, Xue J, Shao J, Jia L (2012) Compilation of 222 drugs\u2019 plasma protein binding data and guidance for study designs. Drug Discov Today 17:475\u2013485","journal-title":"Drug Discov Today"},{"key":"9223_CR45","volume-title":"Goodman & Gilman\u2019s the pharmacological basis of therapeutics","author":"JG Hardman","year":"2001","unstructured":"Hardman JG, Limbird LE, Gilman AG (2001) Goodman & Gilman\u2019s the pharmacological basis of therapeutics, 10th edn. McGraw-Hill, New York","edition":"10"},{"key":"9223_CR46","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.1046\/j.1471-4159.2000.751320.x","volume":"75","author":"B Li","year":"2000","unstructured":"Li B, Stribley JA, Ticu A, Xie W, Schopfer LM, Hammond P, Brimijoin S, Hinrichs SH, Lockridge O (2000) Abundant tissue butyrylcholinesterase and its possible function in the acetylcholinesterase knockout mouse. J Neurochem 75:1320\u20131331","journal-title":"J Neurochem"},{"key":"9223_CR47","doi-asserted-by":"publisher","first-page":"369","DOI":"10.3109\/00498251003706598","volume":"40","author":"H Eng","year":"2010","unstructured":"Eng H, Niosi M, McDonald TS, Wolford A, Chen Y, Simila ST, Bauman JN, Warmus J, Kalgutkar AS (2010) Utility of the carboxylesterase inhibitor bis-para-nitrophenylphosphate (BNPP) in the plasma unbound fraction determination for a hydrolytically unstable amide derivative and agonist of the TGR5 receptor. Xenobiotica 40:369\u2013380","journal-title":"Xenobiotica"},{"key":"9223_CR48","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1517\/13543776.2011.586339","volume":"21","author":"MJ Hatfield","year":"2011","unstructured":"Hatfield MJ, Potter PM (2011) Carboxylesterase inhibitors. Expert Opin Ther Pat 21:1159\u20131171","journal-title":"Expert Opin Ther Pat"},{"key":"9223_CR49","doi-asserted-by":"publisher","first-page":"6P","DOI":"10.1677\/joe.0.0640201","volume":"64","author":"BA Morris","year":"1975","unstructured":"Morris BA, Robinson JD, Piall E, Aherne GW, Marks V (1975) Proceedings: development of a radioimmunoassay for morphine having minimal cross-reactivity with codeine. J Endocrinol 64:6P\u20137P","journal-title":"J Endocrinol"},{"key":"9223_CR50","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1093\/clinchem\/27.9.1524","volume":"27","author":"JW Findlay","year":"1981","unstructured":"Findlay JW, Butz RF, Jones EC (1981) Relationships between immunogen structure and antisera specificity in the narcotic alkaloid series. Clin Chem 27:1524\u20131535","journal-title":"Clin Chem"},{"key":"9223_CR51","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/s004140050190","volume":"112","author":"J Beike","year":"1999","unstructured":"Beike J, Blaschke G, Mertz A, Kohler H, Brinkmann B (1999) A specific immunoassay for the determination of morphine and its glucuronides in human blood. Int J Legal Med 112:8\u201314","journal-title":"Int J Legal Med"},{"key":"9223_CR52","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/0022-1759(93)90080-Q","volume":"157","author":"T Usagawa","year":"1993","unstructured":"Usagawa T, Itoh Y, Hifumi E, Takeyasu A, Nakahara Y, Uda T (1993) Characterization of morphine-specific monoclonal antibodies showing minimal cross-reactivity with codeine. J Immunol Methods 157:143\u2013148","journal-title":"J Immunol Methods"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-015-9223-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00216-015-9223-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-015-9223-z","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-015-9223-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T20:18:02Z","timestamp":1748722682000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00216-015-9223-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,16]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["9223"],"URL":"https:\/\/doi.org\/10.1007\/s00216-015-9223-z","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,16]]},"assertion":[{"value":"25 September 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 November 2015","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 November 2015","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 December 2015","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no competing interests. However, Gary R. Matyas, Kenner C. Rice, Arthur E. Jacobson, and Carl R. Alving are coinventors in a related patent application that is owned by Walter Reed Army Institute of Research, National Institute on Drug Abuse, and Henry M. Jackson Foundation.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The views expressed in this article are those of the authors and do not necessarily reflect the official policy of the Department of the Army, Department of Defense, or NIH, or the U.S. Government.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclaimer"}}]}}