{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T18:11:59Z","timestamp":1778350319321,"version":"3.51.4"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"23","license":[{"start":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T00:00:00Z","timestamp":1565740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T00:00:00Z","timestamp":1565740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774322"],"award-info":[{"award-number":["81774322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2019,9,1]]},"DOI":"10.1007\/s00216-019-02010-x","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T07:02:40Z","timestamp":1565766160000},"page":"6189-6202","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Enhancing coverage in LC\u2013MS-based untargeted metabolomics by a new sample preparation procedure using mixed-mode solid-phase extraction and two derivatizations"],"prefix":"10.1007","volume":"411","author":[{"given":"Qian","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongchao","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhimin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,8,14]]},"reference":[{"issue":"2","key":"2010_CR1","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1007\/s11306-012-0482-9","volume":"9","author":"J Xia","year":"2013","unstructured":"Xia J, Broadhurst DI, Wilson M, Wishart DS. Translational biomarker discovery in clinical metabolomics: an introductory tutorial. Metabolomics. 2013;9(2):280\u201399. \n                    https:\/\/doi.org\/10.1007\/s11306-012-0482-9\n                    \n                  .","journal-title":"Metabolomics."},{"issue":"2","key":"2010_CR2","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.semnephrol.2018.01.005","volume":"38","author":"EP Rhee","year":"2018","unstructured":"Rhee EP. A systems-level view of renal metabolomics. Semin Nephrol. 2018;38(2):142\u201350. \n                    https:\/\/doi.org\/10.1016\/j.semnephrol.2018.01.005\n                    \n                  .","journal-title":"Semin Nephrol"},{"key":"2010_CR3","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.ctrv.2018.04.012","volume":"67","author":"A McCartney","year":"2018","unstructured":"McCartney A, Vignoli A, Biganzoli L, Love R, Tenori L, Luchinat C, et al. Metabolomics in breast cancer: a decade in review. Cancer Treat Rev. 2018;67:88\u201396. \n                    https:\/\/doi.org\/10.1016\/j.ctrv.2018.04.012\n                    \n                  .","journal-title":"Cancer Treat Rev"},{"issue":"4","key":"2010_CR4","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1007\/s11306-014-0752-9","volume":"11","author":"D Ryan","year":"2015","unstructured":"Ryan D, Newnham ED, Prenzler PD, Gibson PR. Metabolomics as a tool for diagnosis and monitoring in coeliac disease. Metabolomics. 2015;11(4):980\u201390. \n                    https:\/\/doi.org\/10.1007\/s11306-014-0752-9\n                    \n                  .","journal-title":"Metabolomics."},{"issue":"4","key":"2010_CR5","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1007\/s11306-016-0990-0","volume":"12","author":"O Turkoglu","year":"2016","unstructured":"Turkoglu O, Zeb A, Graham S, Szyperski T, Szender JB, Odunsi K, et al. Metabolomics of biomarker discovery in ovarian cancer: a systematic review of the current literature. Metabolomics. 2016;12(4):60. \n                    https:\/\/doi.org\/10.1007\/s11306-016-0990-0\n                    \n                  .","journal-title":"Metabolomics."},{"issue":"10\u201311","key":"2010_CR6","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1016\/j.clinbiochem.2014.05.053","volume":"47","author":"LK Lee","year":"2014","unstructured":"Lee LK, Foo KY. Recent insights on the significance of transcriptomic and metabolomic analysis of male factor infertility. Clin Biochem. 2014;47(10\u201311):973\u201382. \n                    https:\/\/doi.org\/10.1016\/j.clinbiochem.2014.05.053\n                    \n                  .","journal-title":"Clin Biochem"},{"issue":"6","key":"2010_CR7","doi-asserted-by":"publisher","first-page":"3088","DOI":"10.1021\/pr5003142","volume":"13","author":"J Zhang","year":"2014","unstructured":"Zhang J, Mu X, Xia Y, Martin FL, Hang W, Liu L, et al. Metabolomic analysis reveals a unique urinary pattern in normozoospermic infertile men. J Proteome Res. 2014;13(6):3088\u201399. \n                    https:\/\/doi.org\/10.1021\/pr5003142\n                    \n                  .","journal-title":"J Proteome Res"},{"issue":"1","key":"2010_CR8","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1021\/acs.analchem.5b04491","volume":"88","author":"T Cajka","year":"2016","unstructured":"Cajka T, Fiehn O. Toward merging untargeted and targeted methods in mass spectrometry-based metabolomics and lipidomics. Anal Chem. 2016;88(1):524\u201345. \n                    https:\/\/doi.org\/10.1021\/acs.analchem.5b04491\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"1","key":"2010_CR9","doi-asserted-by":"publisher","first-page":"S144","DOI":"10.1007\/s11306-012-0412-x","volume":"9","author":"MR Viant","year":"2013","unstructured":"Viant MR, Sommer U. Mass spectrometry based environmental metabolomics: a primer and review. Metabolomics. 2013;9(1):S144\u2013S58. \n                    https:\/\/doi.org\/10.1007\/s11306-012-0412-x\n                    \n                  .","journal-title":"Metabolomics."},{"issue":"14","key":"2010_CR10","doi-asserted-by":"publisher","first-page":"3408","DOI":"10.1039\/C8AN00490K","volume":"143","author":"T Huang","year":"2018","unstructured":"Huang T, Toro M, Lee R, Hui DS, Edwards JL. Multi-functional derivatization of amine, hydroxyl, and carboxylate groups for metabolomic investigations of human tissue by electrospray ionization mass spectrometry. Analyst. 2018;143(14):3408\u201314. \n                    https:\/\/doi.org\/10.1039\/C8AN00490K\n                    \n                  .","journal-title":"Analyst."},{"issue":"21","key":"2010_CR11","doi-asserted-by":"publisher","first-page":"8789","DOI":"10.1021\/ac102146g","volume":"82","author":"K Guo","year":"2010","unstructured":"Guo K, Li L. High-performance isotope labeling for profiling carboxylic acid-containing metabolites in biofluids by mass spectrometry. Anal Chem. 2010;82(21):8789\u201393. \n                    https:\/\/doi.org\/10.1021\/ac102146g\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"1","key":"2010_CR12","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.steroids.2006.10.005","volume":"72","author":"K Yamashita","year":"2007","unstructured":"Yamashita K, Kobayashi S, Tsukamoto S, Numazawa M. Synthesis of pyridine-carboxylate derivatives of hydroxysteroids for liquid chromatography\u2013electrospray ionization-mass spectrometry. Steroids. 2007;72(1):50\u20139. \n                    https:\/\/doi.org\/10.1016\/j.steroids.2006.10.005\n                    \n                  .","journal-title":"Steroids."},{"issue":"12","key":"2010_CR13","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1016\/j.steroids.2009.06.007","volume":"74","author":"K Yamashita","year":"2009","unstructured":"Yamashita K, Miyashiro Y, Maekubo H, Okuyama M, Honma S, Takahashi M, et al. Development of highly sensitive quantification method for testosterone and dihydrotestosterone in human serum and prostate tissue by liquid chromatography\u2013electrospray ionization tandem mass spectrometry. Steroids. 2009;74(12):920\u20136. \n                    https:\/\/doi.org\/10.1016\/j.steroids.2009.06.007\n                    \n                  .","journal-title":"Steroids."},{"issue":"6","key":"2010_CR14","first-page":"482","volume":"30","author":"G Astarita","year":"2012","unstructured":"Astarita G. New frontiers for mass spectrometry in lipidomics, part II. LC GC North Am. 2012;30(6):482.","journal-title":"LC GC North Am"},{"key":"2010_CR15","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.chroma.2013.04.055","volume":"1297","author":"F Gritti","year":"2013","unstructured":"Gritti F, Guiochon G. Comparison between the intra-particle diffusivity in the hydrophilic interaction chromatography and reversed phase liquid chromatography modes. Impact on the column efficiency. J Chromatogr A. 2013;1297:85\u201395. \n                    https:\/\/doi.org\/10.1016\/j.chroma.2013.04.055\n                    \n                  .","journal-title":"J Chromatogr A"},{"issue":"6","key":"2010_CR16","doi-asserted-by":"publisher","first-page":"1684","DOI":"10.1074\/mcp.M114.046508","volume":"14","author":"K Contrepois","year":"2015","unstructured":"Contrepois K, Jiang L, Snyder M. Optimized analytical procedures for the untargeted metabolomic profiling of human urine and plasma by combining hydrophilic interaction (HILIC) and reverse-phase liquid chromatography (RPLC)\u2013mass spectrometry. Mol Cell Proteomics. 2015;14(6):1684\u201395. \n                    https:\/\/doi.org\/10.1074\/mcp.M114.046508\n                    \n                  .","journal-title":"Mol Cell Proteomics"},{"key":"2010_CR17","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1016\/j.talanta.2018.01.084","volume":"182","author":"AJ Chetwynd","year":"2018","unstructured":"Chetwynd AJ, David A. A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage. Talanta. 2018;182:380\u201390. \n                    https:\/\/doi.org\/10.1016\/j.talanta.2018.01.084\n                    \n                  .","journal-title":"Talanta."},{"issue":"12","key":"2010_CR18","doi-asserted-by":"publisher","first-page":"7608","DOI":"10.1021\/acs.analchem.8b01331","volume":"90","author":"Q Xuan","year":"2018","unstructured":"Xuan Q, Hu C, Yu D, Wang L, Zhou Y, Zhao X, et al. Development of a high coverage pseudotargeted lipidomics method based on ultra-high performance liquid chromatography-mass spectrometry. Anal Chem. 2018;90(12):7608\u201316. \n                    https:\/\/doi.org\/10.1021\/acs.analchem.8b01331\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"12","key":"2010_CR19","doi-asserted-by":"publisher","first-page":"6758","DOI":"10.1021\/acs.analchem.7b01098","volume":"89","author":"S Zhao","year":"2017","unstructured":"Zhao S, Dawe M, Guo K, Li L. Development of high-performance chemical isotope labeling LC\u2013MS for profiling the carbonyl submetabolome. Anal Chem. 2017;89(12):6758\u201365. \n                    https:\/\/doi.org\/10.1021\/acs.analchem.7b01098\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"21","key":"2010_CR20","doi-asserted-by":"publisher","first-page":"10617","DOI":"10.1021\/acs.analchem.6b02967","volume":"88","author":"S Zhao","year":"2016","unstructured":"Zhao S, Luo X, Li L. Chemical isotope labeling LC-MS for high coverage and quantitative profiling of the hydroxyl submetabolome in metabolomics. Anal Chem. 2016;88(21):10617\u201323. \n                    https:\/\/doi.org\/10.1021\/acs.analchem.6b02967\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"17","key":"2010_CR21","doi-asserted-by":"publisher","first-page":"5033","DOI":"10.1007\/s00216-015-8528-2","volume":"407","author":"M Hol\u010dapek","year":"2015","unstructured":"Hol\u010dapek M, Ov\u010da\u010d\u00edkov\u00e1 M, L\u00edsa M, C\u00edfkov\u00e1 E, H\u00e1jek T. Continuous comprehensive two-dimensional liquid chromatography\u2013electrospray ionization mass spectrometry of complex lipidomic samples. Anal Bioanal Chem. 2015;407(17):5033\u201343. \n                    https:\/\/doi.org\/10.1007\/s00216-015-8528-2\n                    \n                  .","journal-title":"Anal Bioanal Chem"},{"key":"2010_CR22","doi-asserted-by":"publisher","first-page":"38885","DOI":"10.1038\/srep38885","volume":"6","author":"DG Sitnikov","year":"2016","unstructured":"Sitnikov DG, Monnin CS, Vuckovic D. Systematic assessment of seven solvent and solid-phase extraction methods for metabolomics analysis of human plasma by LC-MS. Sci Rep. 2016;6:38885. \n                    https:\/\/doi.org\/10.1038\/srep38885\n                    \n                  .","journal-title":"Sci Rep"},{"issue":"6","key":"2010_CR23","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1002\/mas.21562","volume":"37","author":"L Cui","year":"2018","unstructured":"Cui L, Lu H, Lee YH. Challenges and emergent solutions for LC-MS\/MS based untargeted metabolomics in diseases. Mass Spectrom Rev. 2018;37(6):772\u201392. \n                    https:\/\/doi.org\/10.1002\/mas.21562\n                    \n                  .","journal-title":"Mass Spectrom Rev"},{"issue":"15","key":"2010_CR24","doi-asserted-by":"publisher","first-page":"5279","DOI":"10.1007\/s00216-013-6927-9","volume":"405","author":"H Bi","year":"2013","unstructured":"Bi H, Krausz KW, Manna SK, Li F, Johnson CH, Gonzalez FJ. Optimization of harvesting, extraction, and analytical protocols for UPLC-ESI-MS-based metabolomic analysis of adherent mammalian cancer cells. Anal Bioanal Chem. 2013;405(15):5279\u201389. \n                    https:\/\/doi.org\/10.1007\/s00216-013-6927-9\n                    \n                  .","journal-title":"Anal Bioanal Chem"},{"issue":"50","key":"2010_CR25","doi-asserted-by":"publisher","first-page":"6728","DOI":"10.1039\/C8CC02592D","volume":"54","author":"D Vuckovic","year":"2018","unstructured":"Vuckovic D. Improving metabolome coverage and data quality: advancing metabolomics and lipidomics for biomarker discovery. Chem Commun. 2018;54(50):6728\u201349. \n                    https:\/\/doi.org\/10.1039\/C8CC02592D\n                    \n                  .","journal-title":"Chem Commun"},{"issue":"1","key":"2010_CR26","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/mas.20108","volume":"26","author":"K Dettmer","year":"2007","unstructured":"Dettmer K, Aronov PA, Hammock BD. Mass spectrometry-based metabolomics. Mass Spectrom Rev. 2007;26(1):51\u201378. \n                    https:\/\/doi.org\/10.1002\/mas.20108\n                    \n                  .","journal-title":"Mass Spectrom Rev"},{"issue":"6","key":"2010_CR27","doi-asserted-by":"publisher","first-page":"1944","DOI":"10.1021\/ac102614v","volume":"83","author":"D Vuckovic","year":"2011","unstructured":"Vuckovic D, Pawliszyn J. Systematic evaluation of solid-phase microextraction coatings for untargeted metabolomic profiling of biological fluids by liquid chromatography\u2212mass spectrometry. Anal Chem. 2011;83(6):1944\u201354. \n                    https:\/\/doi.org\/10.1021\/ac102614v\n                    \n                  .","journal-title":"Anal Chem"},{"key":"2010_CR28","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.chroma.2014.09.001","volume":"1365","author":"A David","year":"2014","unstructured":"David A, Abdul-Sada A, Lange A, Tyler CR, Hill EM. A new approach for plasma (xeno)metabolomics based on solid-phase extraction and nanoflow liquid chromatography-nanoelectrospray ionisation mass spectrometry. J Chromatogr A. 2014;1365:72\u201385. \n                    https:\/\/doi.org\/10.1016\/j.chroma.2014.09.001\n                    \n                  .","journal-title":"J Chromatogr A"},{"key":"2010_CR29","doi-asserted-by":"publisher","unstructured":"Cha E, Lee KM, Park KD, Park KS, Lee K-W, Kim S-M, et al. Hydroxycholesterol levels in the serum and cerebrospinal fluid of patients with neuromyelitis optica revealed by LC-Ag+CIS\/MS\/MS and LC-ESI\/MS\/MS with picolinic derivatization: increased levels and association with disability during acute attack. PLoS One. 2016;11(12). \n                    https:\/\/doi.org\/10.1371\/journal.pone.0167819\n                    \n                  .","DOI":"10.1371\/journal.pone.0167819"},{"issue":"11","key":"2010_CR30","doi-asserted-by":"publisher","first-page":"5988","DOI":"10.1021\/acs.analchem.6b01021","volume":"88","author":"Q Wu","year":"2016","unstructured":"Wu Q, Comi TJ, Li B, Rubakhin SS, Sweedler JV. On-tissue derivatization via electrospray deposition for matrix assisted laser desorption\/ionization mass spectrometry imaging of endogenous fatty acids in rat brain tissues. Anal Chem. 2016;88(11):5988\u201395. \n                    https:\/\/doi.org\/10.1021\/acs.analchem.6b01021\n                    \n                  .","journal-title":"Anal Chem"},{"key":"2010_CR31","doi-asserted-by":"publisher","unstructured":"Pluskal T, Castillo S, Villar-Briones A, Oresic M. MZmine 2: modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data. BMC Bioinformatics. 2010;11(395). \n                    https:\/\/doi.org\/10.1186\/1471-2105-11-395\n                    \n                  .","DOI":"10.1186\/1471-2105-11-395"},{"key":"2010_CR32","doi-asserted-by":"publisher","first-page":"W652","DOI":"10.1093\/nar\/gkp356","volume":"37","author":"J Xia","year":"2009","unstructured":"Xia J, Psychogios N, Young N, Wishart DS. MetaboAnalyst: a web server for metabolomic data analysis and interpretation. Nucleic Acids Res. 2009;37:W652\u201360. \n                    https:\/\/doi.org\/10.1093\/nar\/gkp356\n                    \n                  .","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"2010_CR33","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3758\/bf03193146","volume":"39","author":"F Faul","year":"2007","unstructured":"Faul F, Erdfelder E, Lang A-G, Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007;39(2):175\u201391. \n                    https:\/\/doi.org\/10.3758\/bf03193146\n                    \n                  .","journal-title":"Behav Res Methods"},{"key":"2010_CR34","doi-asserted-by":"publisher","first-page":"D521","DOI":"10.1093\/nar\/gkl923","volume":"35","author":"DS Wishart","year":"2007","unstructured":"Wishart DS, Tzur D, Knox C, Eisner R, Guo AC, Young N, et al. HMDB: the Human Metabolome Database. Nucleic Acids Res. 2007;35:D521\u20136. \n                    https:\/\/doi.org\/10.1093\/nar\/gkl923\n                    \n                  .","journal-title":"Nucleic Acids Res"},{"key":"2010_CR35","doi-asserted-by":"publisher","first-page":"D527","DOI":"10.1093\/nar\/gkl838","volume":"35","author":"M Sud","year":"2007","unstructured":"Sud M, Fahy E, Cotter D, Brown A, Dennis EA, Glass CK, et al. LMSD: LIPID MAPS structure database. Nucleic Acids Res. 2007;35:D527\u201332. \n                    https:\/\/doi.org\/10.1093\/nar\/gkl838\n                    \n                  .","journal-title":"Nucleic Acids Res"},{"issue":"16","key":"2010_CR36","doi-asserted-by":"publisher","first-page":"7946","DOI":"10.1021\/acs.analchem.6b00770","volume":"88","author":"H Tsugawa","year":"2016","unstructured":"Tsugawa H, Kind T, Nakabayashi R, Yukihira D, Tanaka W, Cajka T, et al. Hydrogen rearrangement rules: computational MS\/MS fragmentation and structure elucidation using MS-FINDER software. Anal Chem. 2016;88(16):7946\u201358. \n                    https:\/\/doi.org\/10.1021\/acs.analchem.6b00770\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"12","key":"2010_CR37","doi-asserted-by":"publisher","first-page":"4313","DOI":"10.1007\/s00216-013-6779-3","volume":"405","author":"W H-x","year":"2013","unstructured":"H-x W, Wang B, Zhou Y, Q-w J. Rapid and sensitive analysis of phthalate metabolites, bisphenol A, and endogenous steroid hormones in human urine by mixed-mode solid-phase extraction, dansylation, and ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry. Anal Bioanal Chem. 2013;405(12):4313\u20139. \n                    https:\/\/doi.org\/10.1007\/s00216-013-6779-3\n                    \n                  .","journal-title":"Anal Bioanal Chem"},{"issue":"1","key":"2010_CR38","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1021\/ac302919t","volume":"85","author":"S Tulipani","year":"2013","unstructured":"Tulipani S, Llorach R, Urpi-Sarda M, Andres-Lacueva C. Comparative analysis of sample preparation methods to handle the complexity of the blood fluid metabolome: when less is more. Anal Chem. 2013;85(1):341\u20138. \n                    https:\/\/doi.org\/10.1021\/ac302919t\n                    \n                  .","journal-title":"Anal Chem"},{"issue":"1","key":"2010_CR39","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.fertnstert.2014.03.033","volume":"102","author":"J Zhang","year":"2014","unstructured":"Zhang J, Huang Z, Chen M, Xia Y, Martin FL, Hang W, et al. Urinary metabolome identifies signatures of oligozoospermic infertile men. Fertil Steril. 2014;102(1):44\u201353.e12. \n                    https:\/\/doi.org\/10.1016\/j.fertnstert.2014.03.033\n                    \n                  .","journal-title":"Fertil Steril"},{"key":"2010_CR40","doi-asserted-by":"publisher","first-page":"827941","DOI":"10.1155\/2015\/827941","volume":"2015","author":"A Gvozdjakova","year":"2015","unstructured":"Gvozdjakova A, Kucharska J, Dubravicky J, Mojto V, Singh RB. Coenzyme Q10, \u03b1-tocopherol, and oxidative stress could be important metabolic biomarkers of male infertility. Dis Markers. 2015;2015:827941. \n                    https:\/\/doi.org\/10.1155\/2015\/827941\n                    \n                  .","journal-title":"Dis Markers"},{"issue":"4","key":"2010_CR41","doi-asserted-by":"publisher","first-page":"e0195279","DOI":"10.1371\/journal.pone.0195279","volume":"13","author":"ALC Velho","year":"2018","unstructured":"Velho ALC, Menezes E, Thu D, Kaya A, Topper E, Moura AA, et al. Metabolomic markers of fertility in bull seminal plasma. PLoS One. 2018;13(4):e0195279. \n                    https:\/\/doi.org\/10.1371\/journal.pone.0195279\n                    \n                  .","journal-title":"PLoS One"},{"issue":"46","key":"2010_CR42","doi-asserted-by":"publisher","first-page":"25974","DOI":"10.1039\/c8ra01897a","volume":"8","author":"FJ Zeng","year":"2018","unstructured":"Zeng FJ, Ji HC, Zhang ZM, Luo JK, Lu HM, Wang Y. Metabolic profiling putatively identifies plasma biomarkers of male infertility using UPLC-ESI-IT-TOFMS. RSC Adv. 2018;8(46):25974\u201382. \n                    https:\/\/doi.org\/10.1039\/c8ra01897a\n                    \n                  .","journal-title":"RSC Adv"},{"issue":"9","key":"2010_CR43","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.1007\/s10815-014-0282-4","volume":"31","author":"V Jayaraman","year":"2014","unstructured":"Jayaraman V, Ghosh S, Sengupta A, Srivastava S, Sonawat HM, Narayan PK. Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy. J Assist Reprod Genet. 2014;31(9):1195\u2013204. \n                    https:\/\/doi.org\/10.1007\/s10815-014-0282-4\n                    \n                  .","journal-title":"J Assist Reprod Genet"},{"key":"2010_CR44","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.cca.2015.09.001","volume":"450","author":"X Zhang","year":"2015","unstructured":"Zhang X, Diao R, Zhu X, Li Z, Cai Z. Metabolic characterization of asthenozoospermia using nontargeted seminal plasma metabolomics. Clin Chim Acta. 2015;450:254\u201361. \n                    https:\/\/doi.org\/10.1016\/j.cca.2015.09.001\n                    \n                  .","journal-title":"Clin Chim Acta"},{"issue":"8","key":"2010_CR45","doi-asserted-by":"publisher","first-page":"e3931","DOI":"10.1002\/bmc.3931","volume":"31","author":"K Gilany","year":"2017","unstructured":"Gilany K, Mani-Varnosfaderani A, Minai-Tehrani A, Mirzajani F, Ghassempour A, Sadeghi MR, et al. Untargeted metabolomic profiling of seminal plasma in nonobstructive azoospermia men: a noninvasive detection of spermatogenesis. Biomed Chromatogr. 2017;31(8):e3931. \n                    https:\/\/doi.org\/10.1002\/bmc.3931\n                    \n                  .","journal-title":"Biomed Chromatogr"},{"issue":"12","key":"2010_CR46","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1016\/S0015-0282(16)42979-1","volume":"28","author":"S Kohengkul","year":"1977","unstructured":"Kohengkul S, Tanphaichitr V, Muangmun V, Tanphaichitr N. Levels of L-carnitine and L-O-acetylcarnitine in normal and infertile human semen: a lower level of L-O-acetylcarnitine in infertile semen. Fertil Steril. 1977;28(12):1333\u20136. \n                    https:\/\/doi.org\/10.1016\/S0015-0282(16)42979-1\n                    \n                  .","journal-title":"Fertil Steril"},{"issue":"5","key":"2010_CR47","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1016\/j.jpba.2011.03.010","volume":"55","author":"A Gupta","year":"2011","unstructured":"Gupta A, Mahdi AA, Ahmad MK, Shukla KK, Bansal N, Jaiswer SP, et al. A proton NMR study of the effect of Mucuna pruriens on seminal plasma metabolites of infertile males. J Pharm Biomed Anal. 2011;55(5):1060\u20136. \n                    https:\/\/doi.org\/10.1016\/j.jpba.2011.03.010\n                    \n                  .","journal-title":"J Pharm Biomed Anal"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-019-02010-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00216-019-02010-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-019-02010-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T23:09:14Z","timestamp":1597273754000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00216-019-02010-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,14]]},"references-count":47,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2019,9,1]]}},"alternative-id":["2010"],"URL":"https:\/\/doi.org\/10.1007\/s00216-019-02010-x","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,14]]},"assertion":[{"value":"11 April 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 June 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 August 2019","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Ethics approval was granted by the Ethics Committee of Xiangya Hospital and written informed consent was obtained from all participants.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}]}}