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QS molecules are involved in motility and cell aggregation mechanisms in diseases such as sepsis. Few biomarkers are currently available to diagnose sepsis, especially in high-risk conditions. The aim of this study was the development of new analytical methods based on liquid chromatography-mass spectrometry for the detection and quantification of QS signalling molecules, including N-acyl homoserine lactones (AHL) and hydroxyquinolones (HQ), in biofluids. Biological samples used in the study were <jats:italic>Pseudomonas aeruginosa<\/jats:italic> bacterial cultures and plasma from patients with sepsis. We developed two MS analytical methods, based on neutral loss (NL) and product ion (PI) experiments, to identify and characterize unknown AHL and HQ molecules. We then established a multiple-reaction-monitoring (MRM) method to quantify specific QS compounds. We validated the HPLC-MS-based approaches (MRM-NL-PI), and data were in accord with the validation guidelines. With the NL and PI MS-based methods, we identified and characterized 3 and 13 unknown AHL and HQ compounds, respectively, in biological samples. One of the newly found AHL molecules was C12-AHL, first quantified in <jats:italic>Pseudomonas aeruginosa<\/jats:italic> bacterial cultures. The MRM quantitation of analytes in plasma from patients with sepsis confirmed the analytical ability of MRM for the quantification of virulence factors during sepsis.<\/jats:p>","DOI":"10.1007\/s00216-020-03040-6","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T06:40:33Z","timestamp":1605681633000},"page":"853-864","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Targeted and untargeted quantification of quorum sensing signalling molecules in bacterial cultures and biological samples via HPLC-TQ MS techniques"],"prefix":"10.1007","volume":"413","author":[{"given":"Federica","family":"Dal Bello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Zorzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Aigotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Medica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito","family":"Fanelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Cantaluppi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Amante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana Teresa","family":"Orlandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Medana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,18]]},"reference":[{"key":"3040_CR1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0065-2164(07)62007-3","volume":"62","author":"Y Turovskiy","year":"2007","unstructured":"Turovskiy Y, Kashtanov D, Paskhover B, Chikindas ML. 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All procedures described in studies were in accordance with the ethical standards of our institutional and\/or national research committee and with the Code of Ethics of the World Medical Association (1964 Helsinki declaration) and its later amendments or comparable ethical standards.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research involving human participants and\/or animals"}},{"value":"All healthy volunteers and patients who took part in the study gave their written informed consent before participation. All studies were performed in accordance with the ethical standards provided in the Declaration of Helsinki. The ethical approval was granted by the Research Ethical Committee (protocol number 0114479 approved the 29th of November 2017 at AOU Citt\u00e0 della Salute e della Scienza di Torino).","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}