{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T16:15:20Z","timestamp":1784218520132,"version":"3.55.0"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"19","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["440605\/2016-4"],"award-info":[{"award-number":["440605\/2016-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1007\/s00216-021-03449-7","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T07:02:51Z","timestamp":1624258971000},"page":"4873-4885","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["NS1 glycoprotein detection in serum and urine as an electrochemical screening immunosensor for dengue and Zika virus"],"prefix":"10.1007","volume":"413","author":[{"given":"Priscila D.","family":"Mendon\u00e7a","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenna K. B.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos V.","family":"Foguel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A. B.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marli T.","family":"Cordeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds M.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto T. A.","family":"Marques","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8314-4218","authenticated-orcid":false,"given":"Rosa F.","family":"Dutra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"key":"3449_CR1","unstructured":"WHO. Dengue: causas, sintomas, tratamento e preven\u00e7\u00e3o. 2019."},{"key":"3449_CR2","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.antiviral.2018.12.016","volume":"163","author":"SP Lim","year":"2019","unstructured":"Lim SP. Dengue drug discovery: Progress, challenges and outlook. Antivir Res. 2019;163:156\u201378.","journal-title":"Antivir Res"},{"issue":"7446","key":"3449_CR3","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","volume":"496","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496(7446):504\u20137.","journal-title":"Nature"},{"key":"3449_CR4","doi-asserted-by":"crossref","unstructured":"Alcal\u00e1 AC, Palomares LA, Ludert JE. Secretion of Nonstructural Protein 1 of Dengue Virus from Infected Mosquito Cells: Facts and Speculations. J Virol [Internet]. 2018; 92(14): e00275\u201318.","DOI":"10.1128\/JVI.00275-18"},{"key":"3449_CR5","doi-asserted-by":"crossref","unstructured":"Ahmed NH, Broor S. Comparison of NS1 antigen detection ELISA, real time RT-PCR and virus isolation for rapid diagnosis of dengue infection in acute phase. J Vector Borne Dis. 2014;51(3):194\u20139.","DOI":"10.4103\/0972-9062.141759"},{"issue":"4","key":"3449_CR6","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1586\/14760584.2016.1129279","volume":"15","author":"JK Lim","year":"2016","unstructured":"Lim JK, Lee Y-S, Wilder-Smith A, Thiry G, Mahoney R, Yoon I-K. Points for consideration for dengue vaccine introduction \u2013 recommendations by the dengue vaccine initiative. Expert Rev Vaccines. 2016;15(4):529\u201338.","journal-title":"Expert Rev Vaccines"},{"issue":"12","key":"3449_CR7","doi-asserted-by":"publisher","first-page":"1687.e7","DOI":"10.1016\/j.cmi.2020.02.037","volume":"26","author":"Y Lustig","year":"2020","unstructured":"Lustig Y, Koren R, Biber A, Zuckerman N, Mendelson E, Schwartz E. Screening and exclusion of Zika virus infection in travellers by an NS1-based ELISA and qRT-PCR. Clin Microbiol Infect. 2020;26(12):1687.e7\u20131687.e11.","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"3449_CR8","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.jcv.2014.08.021","volume":"61","author":"EM Korhonen","year":"2014","unstructured":"Korhonen EM, Huhtamo E, Virtala AMK, Kantele A, Vapalahti O. Approach to non-invasive sampling in dengue diagnostics: exploring virus and NS1 antigen detection in saliva and urine of travelers with dengue. J Clin Virol. 2014;61(3):353\u20138.","journal-title":"J Clin Virol"},{"issue":"2","key":"3449_CR9","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1139\/cjm-2019-0331","volume":"66","author":"V Sharma","year":"2020","unstructured":"Sharma V, Sharma M, Dhull D, Sharma Y, Kaushik S, Kaushik S. Zika virus: an emerging challenge to public health worldwide. Can J Microbiol. 2020;66(2):87\u201398.","journal-title":"Can J Microbiol"},{"key":"3449_CR10","unstructured":"Bosch I, De Puig H, Hiley M, Carr\u00e9-Camps M, Perdomo-Celis F, Narv\u00e1ez CF, et al. Rapid antigen tests for dengue virus serotypes and zika virus in patient serum. Sci Transl Med [Internet]. 2017 Sep 27 [cited 2021 Jan 25];9(409). Available from: \/pmc\/articles\/PMC6612058\/?report=abstract."},{"issue":"15","key":"3449_CR11","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.1056\/NEJMra1808246","volume":"381","author":"D Musso","year":"2019","unstructured":"Musso D, Ko AI, Baud D. Zika virus infection \u2014 after the pandemic. N Engl J Med. 2019;381(15):1444\u201357.","journal-title":"N Engl J Med"},{"issue":"2","key":"3449_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/mBio.00095-18","volume":"9","author":"N Mishra","year":"2018","unstructured":"Mishra N, Caciula A, Price A, Thakkar R, Ng J, Chauhan L, et al. Diagnosis of Zika virus infection by peptide array and ELISA. MBio. 2018;9(2):1\u201316.","journal-title":"MBio"},{"key":"3449_CR13","doi-asserted-by":"crossref","unstructured":"Borges ED, Vireque AA, Berteli TS, Ferreira CR, Silva AS, Navarro PA. An update on the aspects of Zika virus infection on male reproductive system. Vol. 36, Journal of Assisted Reproduction and Genetics. Springer New York LLC; 2019. p. 1339\u201349.","DOI":"10.1007\/s10815-019-01493-y"},{"key":"3449_CR14","doi-asserted-by":"crossref","unstructured":"Camara AR, Gouv\u00eaa PMP, Dias ACMS, Braga AMB, Dutra RF, De Araujo RE, et al. Dengue immunoassay with an LSPR fiber optic sensor. Opt Express. 2013;21(22).","DOI":"10.1364\/OE.21.027023"},{"key":"3449_CR15","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1016\/j.jpba.2017.11.064","volume":"149","author":"NT Darwish","year":"2018","unstructured":"Darwish NT, Sekaran SD, Alias Y, Khor SM. Immunofluorescence\u2013based biosensor for the determination of dengue virus NS1 in clinical samples. J Pharm Biomed Anal. 2018;149:591\u2013602.","journal-title":"J Pharm Biomed Anal"},{"key":"3449_CR16","doi-asserted-by":"crossref","unstructured":"Cecchetto J, Carvalho FC, Santos A, Fernandes FCB, Bueno PR. An impedimetric biosensor to test neat serum for dengue diagnosis. Vol. 213, Sensors and Actuators B: Chemical. 2015.","DOI":"10.1016\/j.snb.2015.02.068"},{"key":"3449_CR17","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.bios.2015.09.048","volume":"77","author":"PD Sinawang","year":"2016","unstructured":"Sinawang PD, Rai V, Ionescu RE, Marks RS. Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein. Biosens Bioelectron. 2016;77:400\u20138.","journal-title":"Biosens Bioelectron"},{"key":"3449_CR18","doi-asserted-by":"publisher","first-page":"100030","DOI":"10.1016\/j.sintl.2020.100030","volume":"1","author":"A Dhal","year":"2020","unstructured":"Dhal A, Kalyani T, Ghorai S, Sahu NK, Jana SK. Recent development of electrochemical immunosensor for the diagnosis of dengue virus NSI protein: A review. Sensors Int. 2020;1:100030.","journal-title":"Sensors Int"},{"key":"3449_CR19","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.solmat.2018.04.015","volume":"183","author":"OP Bajpai","year":"2018","unstructured":"Bajpai OP, Kumar S, Bhandari S, Dhar A, Khastgir D, Chattopadhyay S. Electrolyte and current density determines the fate of electrodeposited polythiophene from waveguide to photovoltaics. Sol Energy Mater Sol Cells. 2018;183:107\u201319.","journal-title":"Sol Energy Mater Sol Cells"},{"key":"3449_CR20","doi-asserted-by":"crossref","unstructured":"Watanabe T, Ohira M, Koizumi Y, Nishiyama H, Tomita I, Inagi S. In-plane growth of poly(3,4-ethylenedioxythiophene) films on a substrate surface by bipolar Electropolymerization. ACS Macro Lett 2018;7(5):551\u20135.","DOI":"10.1021\/acsmacrolett.8b00170"},{"key":"3449_CR21","doi-asserted-by":"crossref","unstructured":"Ferreira PAB, Araujo MCM, Prado CM, de Lima RA, Rodr\u00edguez BAG, Dutra RF. An ultrasensitive cystatin C renal failure immunosensor based on a PPy\/CNT electrochemical capacitor grafted on interdigitated electrode. Colloids Surfaces B Biointerfaces 2020;189.","DOI":"10.1016\/j.colsurfb.2020.110834"},{"issue":"35","key":"3449_CR22","doi-asserted-by":"publisher","first-page":"14289","DOI":"10.1039\/C4TA02159B","volume":"2","author":"HJ Salavagione","year":"2014","unstructured":"Salavagione HJ, D\u00edez-Pascual AM, L\u00e1zaro E, Vera S, G\u00f3mez-Fatou MA. Chemical sensors based on polymer composites with carbon nanotubes and graphene: the role of the polymer. J Mater Chem A. 2014;2(35):14289\u2013328.","journal-title":"J Mater Chem A"},{"key":"3449_CR23","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/j.electacta.2017.12.137","volume":"263","author":"MI Mangione","year":"2018","unstructured":"Mangione MI, Spanevello RA, Minudri D, Cavallo P, Otero L, Fungo F. Electrochemical films deposition and electro-optical properties of bis-carbazol-triphenylamine end-caped dendrimeric polymers. Electrochim Acta. 2018;263:585\u201395.","journal-title":"Electrochim Acta"},{"key":"3449_CR24","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/j.talanta.2016.08.011","volume":"160","author":"S Duan","year":"2016","unstructured":"Duan S, Yue R, Huang Y. Polyethylenimine-carbon nanotubes composite as an electrochemical sensing platform for silver nanoparticles. Talanta. 2016;160:607\u201313.","journal-title":"Talanta."},{"key":"3449_CR25","doi-asserted-by":"crossref","unstructured":"Mattos AB, Freitas TA, Kubota LT, Dutra RF. An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum. Biochem Eng J. 2013;71.","DOI":"10.1016\/j.bej.2012.12.005"},{"key":"3449_CR26","doi-asserted-by":"crossref","unstructured":"Dias ACMS, Gomes-Filho SLR, Silva MMS, Dutra RF. A sensor tip based on carbon nanotube-ink printed electrode for the dengue virus NS1 protein. Biosens Bioelectron [Internet]. 2013 15 [cited 2017 Dec 11];44:216\u201321. Available from: https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566312008895","DOI":"10.1016\/j.bios.2012.12.033"},{"issue":"1\u20133","key":"3449_CR27","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.theochem.2004.06.042","volume":"685","author":"S Lakard","year":"2004","unstructured":"Lakard S, Herlem G, Lakard B, Fahys B. Theoretical study of the vibrational spectra of polyethylenimine and polypropylenimine. J Mol Struct {THEOCHEM}. 2004;685(1\u20133):83\u20137.","journal-title":"J Mol Struct"},{"issue":"19\u201320","key":"3449_CR28","doi-asserted-by":"publisher","first-page":"2180","DOI":"10.1016\/j.synthmet.2010.08.005","volume":"160","author":"T Patois","year":"2010","unstructured":"Patois T, Lakard B, Martin N, Fievet P. Effect of various parameters on the conductivity of free standing electrosynthesized polypyrrole films. Synth Met. 2010;160(19\u201320):2180\u20135.","journal-title":"Synth Met"},{"key":"3449_CR29","doi-asserted-by":"crossref","unstructured":"da Fonseca AR, Franco DL, Cordeiro MT, de Oliveira EM, Fireman Dutra RA, Del Pilar Taboada Sotomayor M. Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode. Sensors Actuators, B Chem. 2019;296.","DOI":"10.1016\/j.snb.2019.126681"},{"issue":"47","key":"3449_CR30","doi-asserted-by":"publisher","first-page":"9037","DOI":"10.1039\/c0cc03961f","volume":"46","author":"C Batchelor-McAuley","year":"2010","unstructured":"Batchelor-McAuley C, Goncalves LM, Xiong L, Barros AA, Compton RG. Controlling voltammetric responses by electrode modification; using adsorbed acetone to switch graphite surfaces between adsorptive and diffusive modes. Chem Commun. 2010;46(47):9037\u20139.","journal-title":"Chem Commun"},{"issue":"33","key":"3449_CR31","doi-asserted-by":"publisher","first-page":"14213","DOI":"10.1021\/jp1046672","volume":"114","author":"LM Gon\u00e7alves","year":"2010","unstructured":"Gon\u00e7alves LM, Batchelor-McAuley C, Barros AA, Compton RG. Electrochemical oxidation of adenine: A mixed adsorption and diffusion response on an edge-plane pyrolytic graphite electrode. J Phys Chem C. 2010;114(33):14213\u20139.","journal-title":"J Phys Chem C"},{"key":"3449_CR32","doi-asserted-by":"crossref","unstructured":"Trindade EKG, Silva BVM, Dutra RF. A probeless and label-free electrochemical immunosensor for cystatin C detection based on ferrocene functionalized-graphene platform. Biosens Bioelectron 2019;138.","DOI":"10.1016\/j.bios.2019.05.016"},{"key":"3449_CR33","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1016\/j.talanta.2014.06.009","volume":"128","author":"MMS Silva","year":"2014","unstructured":"Silva MMS, Dias ACMS, Cordeiro MT, Marques E, Goulart MOF, Dutra RF. A thiophene-modified screen printed electrode for detection of dengue virus NS1 protein. Talanta. 2014;128:505\u201310.","journal-title":"Talanta"},{"issue":"8","key":"3449_CR34","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1016\/S0008-6223(01)00266-4","volume":"40","author":"JH Chen","year":"2002","unstructured":"Chen JH, Li WZ, Wang DZ, Yang SX, Wen JG, Ren ZF. Electrochemical characterization of carbon nanotubes as electrode in electrochemical double-layer capacitors. Carbon N Y. 2002;40(8):1193\u20137.","journal-title":"Carbon N Y"},{"key":"3449_CR35","doi-asserted-by":"crossref","unstructured":"Hoefer M, Bandaru PR. Determination and enhancement of the capacitance contributions in carbon nanotube based electrode systems. 2009 [cited 2018 Dec 12]; Available from: http:\/\/maecourses.ucsd.edu\/groups\/bandaru\/Publications_files\/APL paper _capacitanc econtributions.pdf","DOI":"10.1063\/1.3258353"},{"key":"3449_CR36","doi-asserted-by":"crossref","unstructured":"Barbosa S, Gozze AB, Lima NF, Batista CL, da Bastos MS, Nicolete VC, et al. Epidemiology of Disappearing Plasmodium vivax Malaria: A Case Study in Rural Amazonia. Rodrigues MM, editor. PLoS Negl Trop Dis [Internet]. 2014 28 [cited 2019 Oct 9];8(8):e3109. Available from: http:\/\/dx.plos.org\/10.1371\/journal.pntd.0003109","DOI":"10.1371\/journal.pntd.0003109"},{"issue":"6","key":"3449_CR37","doi-asserted-by":"publisher","first-page":"803","DOI":"10.2494\/photopolymer.29.803","volume":"29","author":"H Song","year":"2016","unstructured":"Song H, Li T, Han Y, Wang Y, Zhang C, Wang Q. Optimizing the polymerization conditions of conductive polypyrrole. J Photopolym Sci Technol. 2016;29(6):803\u20138.","journal-title":"J Photopolym Sci Technol"},{"key":"3449_CR38","doi-asserted-by":"crossref","unstructured":"Mundinamani SP, Rabinal MK. Cyclic Voltammetric Studies on the Role of Electrode, Electrode Surface Modification and Electrolyte Solution of an Electrochemical Cell [Internet]. Vol. 7, IOSR Journal of Applied Chemistry (IOSR-JAC. 2014 [cited 2019 Jun 2]. Available from: www.iosrjournals.orgwww.iosrjournals.org","DOI":"10.9790\/5736-07924552"},{"key":"3449_CR39","doi-asserted-by":"crossref","unstructured":"Gomes-Filho SLR, Dias ACMS, Silva MMS, Silva BVM, Dutra RF. A carbon nanotube-based electrochemical immunosensor for cardiac troponin T. Microchem J [Internet]. 2013 Jul [cited 2018 May 16];109:10\u20135. Available from: http:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0026265X12001518","DOI":"10.1016\/j.microc.2012.05.033"},{"key":"3449_CR40","doi-asserted-by":"crossref","unstructured":"Trindade EKG, Dutra RF. A label-free and reagentless immunoelectrode for antibodies against hepatitis B core antigen (anti-HBc) detection. Colloids Surfaces B Biointerfaces [Internet]. 2018 Dec 1 [cited 2019 mar 21];172:272\u20139. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30173094","DOI":"10.1016\/j.colsurfb.2018.08.050"},{"key":"3449_CR41","unstructured":"Silverstein RM, Webster FX, Kiemle DJ, Bryce DL. Spectrometric Identification of Organic Compounds. Wiley; 2014."},{"issue":"11","key":"3449_CR42","doi-asserted-by":"publisher","first-page":"6003","DOI":"10.1166\/jnn.2008.466","volume":"8","author":"MC Rodr\u00edguez","year":"2008","unstructured":"Rodr\u00edguez MC, Rubianes MD, Rivas GA. Highly selective determination of dopamine in the presence of ascorbic acid and serotonin at glassy carbon electrodes modified with carbon nanotubes dispersed in polyethylenimine. J Nanosci Nanotechnol. 2008;8(11):6003\u20139.","journal-title":"J Nanosci Nanotechnol"},{"key":"3449_CR43","doi-asserted-by":"crossref","unstructured":"Murugan E, RC Akshata, Stephy A. Electrochemical sensing of anti-cancer drug (capecitabine) by GC-MWCNT-PAMAM (G3)-AuNps electrode. Adv Mater Proc 2017;2(3):176\u2013183.","DOI":"10.5185\/amp.2017\/309"},{"key":"3449_CR44","unstructured":"Pavia DL, Lampman GM, Kriz GS, Vyvyan JR. Introdu\u00e7\u00e3o \u00e0 Espectroscopia. Cengage; 2015."},{"issue":"3","key":"3449_CR45","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1039\/B9NR00257J","volume":"2","author":"G Zou","year":"2010","unstructured":"Zou G, Jain M, Yang H, Zhang Y, Williams D, Jia Q. Recyclable and electrically conducting carbon nanotube composite films. Nanoscale. 2010;2(3):418\u201322.","journal-title":"Nanoscale"},{"key":"3449_CR46","doi-asserted-by":"crossref","unstructured":"Chen Q, Xu S, Liu Q, Masliyah J, Xu Z. QCM-D study of nanoparticle interactions. Vol. 233, Advances in Colloid and Interface Science. Elsevier B.V.; 2016. p. 94\u2013114.","DOI":"10.1016\/j.cis.2015.10.004"},{"key":"3449_CR47","unstructured":"Rivera-Nieblas JO, Alvarado-Rivera J, Acosta-Enr\u00edquez MC, Ochoa-Landin R, Espinoza-Beltr\u00e1n FJ, Apolinar-Iribe A, et al. resistance and resistivities of pbs thin films using polyethylenimine by chemical bath deposition. Vol. 10, Chalcogenide Letters. 2013."},{"issue":"2","key":"3449_CR48","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1007\/BF01337937","volume":"155","author":"G Sauerbrey","year":"1959","unstructured":"Sauerbrey G. Verwendung von Schwingquarzen zur W{\u00e4}gung d{\u00fc}nner Schichten und zur Mikrow{\u00e4}gung. Zeitschrift f{\u00fc}r Phys. 1959;155(2):206\u201322.","journal-title":"Zeitschrift f{\u00fc}r Phys"},{"key":"3449_CR49","doi-asserted-by":"crossref","unstructured":"Parkash O, Yean C, Shueb R. Screen Printed Carbon Electrode Based Electrochemical Immunosensor for the Detection of Dengue NS1 Antigen. Diagnostics [Internet]. 2014 Nov 20 [cited 2021 Feb 2];4(4):165\u201380. Available from: http:\/\/www.mdpi.com\/2075-4418\/4\/4\/165","DOI":"10.3390\/diagnostics4040165"},{"key":"3449_CR50","doi-asserted-by":"publisher","unstructured":"Nawaz MH, Hayat A, Catanante G, Latif U, Marty JL. Development of a portable and disposable NS1 based electrochemical immunosensor for early diagnosis of dengue virus. Anal Chim Acta [Internet]. 2018 Oct 5 [cited 2021 Feb 4];1026:1\u20137. Available from: https:\/\/doi.org\/10.1016\/j.aca.2018.04.032","DOI":"10.1016\/j.aca.2018.04.032"},{"issue":"9966","key":"3449_CR51","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/S0140-6736(14)60572-9","volume":"385","author":"MG Guzman","year":"2015","unstructured":"Guzman MG, Harris E. Dengue Lancet. 2015;385(9966):453\u201365.","journal-title":"Dengue Lancet"},{"key":"3449_CR52","doi-asserted-by":"crossref","unstructured":"Muller DA, Young PR. The flavivirus NS1 protein: Molecular and structural biology, immunology, role inpathogenesis and application asadiagnostic biomarker. [Internet]. Vol. 98, Antiviral Research. Elsevier B.V.; 2013 [cited 2021 Jan 24]. p. 192\u2013208. Available from: https:\/\/pubmed.ncbi.nlm.nih.gov\/23523765\/","DOI":"10.1016\/j.antiviral.2013.03.008"},{"issue":"February","key":"3449_CR53","doi-asserted-by":"publisher","first-page":"i657","DOI":"10.1136\/bmj.i657","volume":"352","author":"A Gulland","year":"2016","unstructured":"Gulland A. Zika virus is a global public health emergency, declares WHO. BMJ. 2016;352(February):i657.","journal-title":"BMJ"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-021-03449-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-021-03449-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-021-03449-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T06:14:22Z","timestamp":1725257662000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-021-03449-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,21]]},"references-count":53,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["3449"],"URL":"https:\/\/doi.org\/10.1007\/s00216-021-03449-7","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,21]]},"assertion":[{"value":"25 March 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2021","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2021","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 June 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was conducted in accordance with the Helsinki Declaration as revised in 2013. The serum samples used in this study are leftover samples from volunteers that signed the informed consent and authorized the use for research purposes. The study protocol was reviewed and approved by the local Ethics Committee on Human Research at the Instituto Aggeu Magalh\u00e3es-Funda\u00e7\u00e3o Oswaldo Cruz (IAM-FIOCRUZ\/Recife\/Brazil) (approval ID 28309414.9.0000.5190).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}