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Not only the adult worms but also their eggs are responsible for health problems. Up to 50% of the eggs produced by the female worms are not excreted with the feces but are trapped in the host tissue, such as the liver, where they provoke immune responses and a change in the lipid profile. We built up a database with 372 infection markers found in livers of<jats:italic>S. mansoni<\/jats:italic>-infected hamsters, using LC\u2013MS\/MS for identification, followed by statistical analysis. Most of them belong to the lipid classes of phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and triglycerides (TGs). We assigned some of these markers to specific anatomical structures by applying high-resolution MALDI MSI to cryosections of hamster liver and generating ion images based on the marker list from the LC\u2013MS\/MS experiments. Furthermore, enrichment and depletion of several markers were visualized.<\/jats:p><jats:p><jats:bold>Graphic Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00216-022-04006-6","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T15:02:53Z","timestamp":1648047773000},"page":"3653-3665","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Changes in the lipid profile of hamster liver after Schistosoma mansoni infection, characterized by mass spectrometry imaging and LC\u2013MS\/MS analysis"],"prefix":"10.1007","volume":"414","author":[{"given":"Katja R.","family":"Wiedemann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Peter Ventura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Gerbig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Roderfeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Quack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph G.","family":"Grevelding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Roeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Spengler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"issue":"9541","key":"4006_CR1","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1016\/S0140-6736(06)69440-3","volume":"368","author":"B Gryseels","year":"2006","unstructured":"Gryseels B, Polman K, Clerinx J, Kestens L. 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Mem I Oswaldo Cruz. 1995;90(2):289\u201392.","journal-title":"Mem I Oswaldo Cruz"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-022-04006-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-022-04006-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-022-04006-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,29]],"date-time":"2023-01-29T21:44:25Z","timestamp":1675028665000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-022-04006-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,23]]},"references-count":47,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["4006"],"URL":"https:\/\/doi.org\/10.1007\/s00216-022-04006-6","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,23]]},"assertion":[{"value":"14 October 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 January 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 March 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 March 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"To keep the <i>S. mansoni<\/i> life cycle, <i>Biomphalaria glabrata<\/i> snails served as intermediate hosts and Syrian hamsters (<i>Mesocricetus auratus<\/i>) as final hosts. The hamster model has been established because it is more permissive for schistosome infection compared to the mouse model, which requires more animals (reduction principle of the 3Rs). Both snails and hamsters were bred in-house (Biomedical Research Center Seltersberg, Giessen, Germany). All animal experiments were conducted in accordance with the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (ETS No 123; revised Appendix A), and they were additionally approved by the Regional Council (Regierungspraesidium) Giessen (V54-19 c 20\/15 c GI 18\/10).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval, source of biological material, and statement on animal welfare"}},{"value":"B.S. and C.G.G. are consultants of TransMIT GmbH, Giessen, Germany. The other authors declare to have no conflicts of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}