{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:05:05Z","timestamp":1789239905272,"version":"build-2803163510"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"21","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bacillus stearothermophilus<\/jats:italic> large fragment (BST<jats:sub>LF<\/jats:sub>) DNA polymerase is reported, isolated on silica via a fused R5 silica-affinity peptide and used in nucleic acid diagnostics. mCherry (mCh), included in the fusion construct, was shown as an efficient fluorescent label to follow the workflow from gene to diagnostic. The R5 immobilisation on silica from cell lysate was consistent with cooperative R5-specific binding of R5<jats:sub>2<\/jats:sub>-mCh-FL-BST<jats:sub>LF<\/jats:sub> or R5<jats:sub>2<\/jats:sub>-mCh-H10-BST<jats:sub>LF<\/jats:sub> fusion proteins followed by non-specific protein binding (including <jats:italic>E. coli<\/jats:italic> native proteins). Higher R5-binding could be achieved in the presence of phosphate, but phosphate residue reduced loop-mediated isothermal amplification (LAMP) performance, possibly blocking sites on the BST<jats:sub>LF<\/jats:sub> for binding of \u03b2- and \u03b3-phosphates of the dNTPs. Quantitative assessment showed that cations (Mg<jats:sup>2+<\/jats:sup> and Mn<jats:sup>2+<\/jats:sup>) that complex the PPi product optimised enzyme activity. In malaria testing, the limit of detection depended on <jats:italic>Plasmodium<\/jats:italic> species and primer set. For example, 1000 copies of <jats:italic>P. knowlesi<\/jats:italic> 18S rRNA could be detected with the P.KNO-LAU primer set with Si-R5<jats:sub>2<\/jats:sub>-mCh-FL-BST<jats:sub>LF<\/jats:sub> ,\u00a0but 10 copies of <jats:italic>P. ovale<\/jats:italic> 18S rRNA could be detected with the P.OVA-HAN primer set\u00a0using the same enzyme. The Si-immobilised BST<jats:sub>LF<\/jats:sub> outperformed the commercial enzyme for four of the nine <jats:italic>Plasmodium<\/jats:italic> LAMP primer sets tested. Si-R5<jats:sub>2<\/jats:sub>-mCh-FL-BST<jats:sub>LF<\/jats:sub> production was transferred from Cambridge to Accra and set up de novo for a trial with clinical samples. Different detection limits were found, targeting the mitochondrial DNA or the 18S rRNA gene for <jats:italic>P. falciparum.<\/jats:italic> The results are discussed in comparison with qPCR and sampling protocol and show that the Si-BST<jats:sub>LF<\/jats:sub> polymerase can be optimised to meet the WHO recommended guidelines.<\/jats:p>\n                <jats:p><jats:bold>Graphical abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00216-022-04131-2","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T08:03:59Z","timestamp":1654243439000},"page":"6309-6326","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis"],"prefix":"10.1007","volume":"414","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7302-8379","authenticated-orcid":false,"given":"Dushanth","family":"Seevaratnam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6928-2363","authenticated-orcid":false,"given":"Felix","family":"Ansah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8415-2727","authenticated-orcid":false,"given":"Yaw","family":"Aniweh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8793-3641","authenticated-orcid":false,"given":"Gordon A.","family":"Awandare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9572-9854","authenticated-orcid":false,"given":"Elizabeth A. H.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"4131_CR1","doi-asserted-by":"publisher","first-page":"2550","DOI":"10.1111\/febs.14128","volume":"284","author":"R Tuteja","year":"2017","unstructured":"Tuteja R. Introduction to the special issue on malaria. FEBS J. 2017;284:2550\u20132. https:\/\/doi.org\/10.1111\/febs.14128.","journal-title":"FEBS J"},{"key":"4131_CR2","volume-title":"World Health Organization","author":"World malaria report","year":"2021","unstructured":"World malaria report. World Health Organization. Geneva: Switzerland; 2021."},{"key":"4131_CR3","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1016\/S0140-6736(04)17446-1","volume":"364","author":"M Amexo","year":"2004","unstructured":"Amexo M, Tolhurst R, Barnish G, Bates I. Malaria misdiagnosis: effects on the poor and vulnerable. Lancet. 2004;364:1896\u20138. https:\/\/doi.org\/10.1016\/S0140-6736(04)17446-1.","journal-title":"Lancet"},{"key":"4131_CR4","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1186\/s12936-018-2481-4","volume":"17","author":"P Berzosa","year":"2018","unstructured":"Berzosa P, de Lucio A, Romay-Barja M, Herrador Z, Gonz\u00e1lez V, Garc\u00eda L, Fern\u00e1ndez-Mart\u00ednez A, Santana-Morales M, Ncogo P, Valladares B, Riloha M, Benito A. Comparison of three diagnostic methods (microscopy, RDT, and PCR) for the detection of malaria parasites in representative samples from Equatorial Guinea. Malar J. 2018;17:333. https:\/\/doi.org\/10.1186\/s12936-018-2481-4.","journal-title":"Malar J"},{"key":"4131_CR5","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1186\/s12936-016-1502-4","volume":"15","author":"RR Ghai","year":"2016","unstructured":"Ghai RR, Thurber MI, El Bakry A, Chapman CA, Goldberg TL. Multi-method assessment of patients with febrile illness reveals over-diagnosis of malaria in rural Uganda. Malar J. 2016;15:460. https:\/\/doi.org\/10.1186\/s12936-016-1502-4.","journal-title":"Malar J"},{"key":"4131_CR6","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1007\/s00216-018-1553-1","volume":"411","author":"R Martzy","year":"2019","unstructured":"Martzy R, Kolm C, Krska R, Mach RL, Farnleitner AH, Reischer GH. Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis. Anal Bioanal Chem. 2019;411:1695\u2013702. https:\/\/doi.org\/10.1007\/s00216-018-1553-1.","journal-title":"Anal Bioanal Chem"},{"key":"4131_CR7","unstructured":"(2020) Public financing for health in Africa: 15% of an elephant is not 15% of a chicken. In: Africa Renewal. https:\/\/www.un.org\/africarenewal\/magazine\/october-2020\/public-financing-health-africa-when-15-elephant-not-15-chicken. Accessed 1 Apr 2022"},{"key":"4131_CR8","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.ijid.2020.07.009","volume":"98","author":"S Picot","year":"2020","unstructured":"Picot S, Cucherat M, Bienvenu A-L. Systematic review and meta-analysis of diagnostic accuracy of loop-mediated isothermal amplification (LAMP) methods compared with microscopy, polymerase chain reaction and rapid diagnostic tests for malaria diagnosis. Int J Infect Dis. 2020;98:408\u201319. https:\/\/doi.org\/10.1016\/j.ijid.2020.07.009.","journal-title":"Int J Infect Dis"},{"key":"4131_CR9","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1186\/s12936-021-03923-8","volume":"20","author":"DG Feleke","year":"2021","unstructured":"Feleke DG, Alemu Y, Yemanebirhane N. Performance of rapid diagnostic tests, microscopy, loop-mediated isothermal amplification (LAMP) and PCR for malaria diagnosis in Ethiopia: a systematic review and meta-analysis. Malar J. 2021;20:384. https:\/\/doi.org\/10.1186\/s12936-021-03923-8.","journal-title":"Malar J"},{"key":"4131_CR10","doi-asserted-by":"publisher","first-page":"6877","DOI":"10.1007\/s00216-019-02059-8","volume":"411","author":"Q Lin","year":"2019","unstructured":"Lin Q, Ye X, Yang B, Fang X, Chen H, Weng W, Kong J. Real-time fluorescence loop-mediated isothermal amplification assay for rapid and sensitive detection of Streptococcus gallolyticus subsp. gallolyticus associated with colorectal cancer. Anal Bioanal Chem. 2019;411:6877\u201387. https:\/\/doi.org\/10.1007\/s00216-019-02059-8.","journal-title":"Anal Bioanal Chem"},{"key":"4131_CR11","doi-asserted-by":"publisher","first-page":"2923","DOI":"10.1007\/s00216-021-03224-8","volume":"413","author":"C Xie","year":"2021","unstructured":"Xie C, Chen S, Zhang L, He X, Ma Y, Wu H, Zou B, Zhou G. Multiplex detection of blood-borne pathogens on a self-driven microfluidic chip using loop-mediated isothermal amplification. Anal Bioanal Chem. 2021;413:2923\u201331. https:\/\/doi.org\/10.1007\/s00216-021-03224-8.","journal-title":"Anal Bioanal Chem"},{"key":"4131_CR12","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1080\/15257770701845204","volume":"27","author":"P Gill","year":"2008","unstructured":"Gill P, Ghaemi A. Nucleic acid isothermal amplification technologies\u2014a review. Nucleosides, Nucleotides Nucleic Acids. 2008;27:224\u201343. https:\/\/doi.org\/10.1080\/15257770701845204.","journal-title":"Nucleosides, Nucleotides Nucleic Acids"},{"key":"4131_CR13","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1039\/C3MB70304E","volume":"10","author":"L Yan","year":"2014","unstructured":"Yan L, Zhou J, Zheng Y, Gamson SA, Roembke TB, Nakayama S, Sintim OH. Isothermal amplified detection of DNA and RNA. Molec BioSyst. 2014;10:970\u20131003. https:\/\/doi.org\/10.1039\/C3MB70304E.","journal-title":"Molec BioSyst"},{"key":"4131_CR14","doi-asserted-by":"publisher","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:E63.","journal-title":"Nucleic Acids Res"},{"key":"4131_CR15","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.biomaterials.2018.12.003","volume":"193","author":"CJ Henderson","year":"2019","unstructured":"Henderson CJ, Pumford E, Seevaratnam DJ, Daly R, Hall EAH. Gene to diagnostic: self immobilizing protein for silica microparticle biosensor, modelled with sarcosine oxidase. Biomaterials. 2019;193:58\u201370. https:\/\/doi.org\/10.1016\/j.biomaterials.2018.12.003.","journal-title":"Biomaterials"},{"key":"4131_CR16","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1038\/nbt1209-1077","volume":"27","author":"J Alper","year":"2009","unstructured":"Alper J. Biotech in the basement. Nat Biotechnol. 2009;27:1077\u20138. https:\/\/doi.org\/10.1038\/nbt1209-1077.","journal-title":"Nat Biotechnol"},{"key":"4131_CR17","volume-title":"Social innovation: what it is, why it matters and how it can be accelerated","author":"G Mulgan","year":"2007","unstructured":"Mulgan G, Tucker S, Ali R, Sanders B, University of Oxford, Skoll Centre for Social Entrepreneurship. Social innovation: what it is, why it matters and how it can be accelerated. London: Young Foundation; 2007."},{"key":"4131_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2020.129088","volume":"329","author":"CJ Henderson","year":"2021","unstructured":"Henderson CJ, Rognin E, Hall EA, Daly R. Design and model for \u2018falling particle\u2019 biosensors. Sens Actuators, B Chem. 2021;329: 129088. https:\/\/doi.org\/10.1016\/j.snb.2020.129088.","journal-title":"Sens Actuators, B Chem"},{"key":"4131_CR19","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1016\/j.snb.2019.02.061","volume":"287","author":"N Jornet-Mart\u00ednez","year":"2019","unstructured":"Jornet-Mart\u00ednez N, Henderson CJ, Camp\u00edns-Falc\u00f3 P, Daly R, Hall EAH. Towards sarcosine determination in urine for prostatic carcinoma detection. Sens Actuators, B Chem. 2019;287:380\u20139. https:\/\/doi.org\/10.1016\/j.snb.2019.02.061.","journal-title":"Sens Actuators, B Chem"},{"key":"4131_CR20","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/0166-6851(93)90077-B","volume":"61","author":"G Snounou","year":"1993","unstructured":"Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN,. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol. 1993;61:315\u201320. https:\/\/doi.org\/10.1016\/0166-6851(93)90077-B.","journal-title":"Mol Biochem Parasitol"},{"key":"4131_CR21","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1111\/mmi.12162","volume":"88","author":"A Heinberg","year":"2013","unstructured":"Heinberg A, Siu E, Stern C, Lawrence EA, Ferdig MT, Deitsch KW, Kirkman LA. Direct evidence for the adaptive role of copy number variation on antifolate susceptibility in Plasmodium falciparum. Mol Microbiol. 2013;88:702\u201312. https:\/\/doi.org\/10.1111\/mmi.12162.","journal-title":"Mol Microbiol"},{"key":"4131_CR22","doi-asserted-by":"publisher","first-page":"81","DOI":"10.2144\/000114198","volume":"57","author":"KB Ignatov","year":"2014","unstructured":"Ignatov KB, Barsova EV, Fradkov AF, Blagodatskikh KA, Kramarova TV, Kramarov VM. A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification. Biotechniques. 2014;57:81\u20137. https:\/\/doi.org\/10.2144\/000114198.","journal-title":"Biotechniques"},{"key":"4131_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/09674845.2015.11666788","volume":"72","author":"MS Kabir","year":"2015","unstructured":"Kabir MS, Clements MO, Kimmitt PT. RT-Bst: an integrated approach for reverse transcription and enrichment of cDNA from viral RNA. Br J Biomed Sci. 2015;72:1\u20136. https:\/\/doi.org\/10.1080\/09674845.2015.11666788.","journal-title":"Br J Biomed Sci"},{"key":"4131_CR24","doi-asserted-by":"publisher","first-page":"1924","DOI":"10.1039\/c7an01897e","volume":"143","author":"RJ Meagher","year":"2018","unstructured":"Meagher RJ, Priye A, Light YK, Huang C, Wang E. Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA. Analyst. 2018;143:1924\u201333. https:\/\/doi.org\/10.1039\/c7an01897e.","journal-title":"Analyst"},{"key":"4131_CR25","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1385\/ABAB:135:1:59","volume":"135","author":"K-M Lee","year":"2006","unstructured":"Lee K-M, Rhee C-H, Kang C-K, Kim J-H. Sequential and simultaneous statistical optimization by dynamic design of experiment for peptide overexpression in recombinant Escherichia coli. Appl Biochem Biotechnol. 2006;135:59\u201380. https:\/\/doi.org\/10.1385\/ABAB:135:1:59.","journal-title":"Appl Biochem Biotechnol"},{"key":"4131_CR26","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1186\/1756-0500-7-671","volume":"7","author":"AL Larentis","year":"2014","unstructured":"Larentis AL, Nicolau JFMQ, Esteves G dos S, Vareschini DT, de Almeida FVR, dos Reis MG, Galler R, Medeiros MA. Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E coli using shaking flasks and microbioreactor. BMC Res Notes. 2014;7:671. https:\/\/doi.org\/10.1186\/1756-0500-7-671.","journal-title":"BMC Res Notes"},{"key":"4131_CR27","doi-asserted-by":"publisher","first-page":"2391","DOI":"10.1016\/j.bpj.2008.12.3902","volume":"96","author":"B Wu","year":"2009","unstructured":"Wu B, Chen Y, M\u00fcller JD. Fluorescence fluctuation spectroscopy of mCherry in living cells. Biophys J. 2009;96:2391\u2013404. https:\/\/doi.org\/10.1016\/j.bpj.2008.12.3902.","journal-title":"Biophys J"},{"key":"4131_CR28","doi-asserted-by":"publisher","first-page":"3533","DOI":"10.1016\/j.bmc.2013.04.006","volume":"21","author":"CC Lechner","year":"2013","unstructured":"Lechner CC, Becker CFW. Modified silaffin R5 peptides enable encapsulation and release of cargo molecules from biomimetic silica particles. Bioorg Med Chem. 2013;21:3533\u201341. https:\/\/doi.org\/10.1016\/j.bmc.2013.04.006.","journal-title":"Bioorg Med Chem"},{"key":"4131_CR29","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0079-6603(08)60889-2","volume-title":"Progress in Nucleic Acid Research and Molecular Biology","author":"BS Negrutskii","year":"1998","unstructured":"Negrutskii BS, El\u2019skaya AV,. Eukaryotic translation elongation factor 1\u03b1: structure, expression, functions, and possible role in aminoacyl-tRNA channeling. In: Moldave K, editor. Progress in Nucleic Acid Research and Molecular Biology. Academic Press; 1998. p. 47\u201378."},{"key":"4131_CR30","doi-asserted-by":"crossref","unstructured":"Harvey KL, Jarocki VM, Charles IG, Djordjevic SP. The diverse functional roles of elongation factor Tu (EF-Tu) in microbial pathogenesis. Front Microbiol 2019;10","DOI":"10.3389\/fmicb.2019.02351"},{"key":"4131_CR31","doi-asserted-by":"publisher","first-page":"5013","DOI":"10.1021\/acs.langmuir.9b00283","volume":"35","author":"B Hellner","year":"2019","unstructured":"Hellner B, Lee SB, Subramaniam A, Subramanian VR, Baneyx F. Modeling the cooperative adsorption of solid-binding proteins on silica: molecular insights from surface plasmon resonance measurements. Langmuir. 2019;35:5013\u201320. https:\/\/doi.org\/10.1021\/acs.langmuir.9b00283.","journal-title":"Langmuir"},{"key":"4131_CR32","doi-asserted-by":"publisher","unstructured":"Bolanos-Garcia VM, Davies OR. Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography. Biochimica et Biophysica Acta (BBA) - General Subjects 2006;1760:1304\u20131313. https:\/\/doi.org\/10.1016\/j.bbagen.2006.03.027","DOI":"10.1016\/j.bbagen.2006.03.027"},{"key":"4131_CR33","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.1128\/JCM.33.4.1036-1038.1995","volume":"33","author":"SR Johnson","year":"1995","unstructured":"Johnson SR, Martin DH, Cammarata C, Morse SA. Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid. J Clin Microbiol. 1995;33:1036\u20138. https:\/\/doi.org\/10.1128\/JCM.33.4.1036-1038.1995.","journal-title":"J Clin Microbiol"},{"key":"4131_CR34","doi-asserted-by":"crossref","unstructured":"Davalieva K, Efremov GD. Influence of salts and pcr inhibitors on the amplification capacity of three thermostable dna polymerases. Maced J Chem Chem Eng 2010;6","DOI":"10.20450\/mjcce.2010.173"},{"key":"4131_CR35","doi-asserted-by":"publisher","first-page":"2607","DOI":"10.1039\/C4TB01679C","volume":"3","author":"L Senior","year":"2015","unstructured":"Senior L, Crump MP, Williams C, Booth PJ, Mann S, Perriman AW, Curnow P. Structure and function of the silicifying peptide R5. J Mater Chem B. 2015;3:2607\u201314.","journal-title":"J Mater Chem B"},{"key":"4131_CR36","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1021\/acsomega.7b01769","volume":"3","author":"TT Olmez","year":"2018","unstructured":"Olmez TT, Yuca E, Eyupoglu E, Catalak HB, Sahin O, Seker UOS. Autonomous synthesis of fluorescent silica biodots using engineered fusion proteins. ACS Omega. 2018;3:585\u201394. https:\/\/doi.org\/10.1021\/acsomega.7b01769.","journal-title":"ACS Omega"},{"key":"4131_CR37","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1002\/psc.2577","volume":"20","author":"CC Lechner","year":"2014","unstructured":"Lechner CC, Becker CF. A sequence-function analysis of the silica precipitating silaffin R5 peptide. J Pept Sci. 2014;20:152\u20138.","journal-title":"J Pept Sci"},{"key":"4131_CR38","doi-asserted-by":"publisher","first-page":"6586","DOI":"10.1016\/j.psj.2020.09.077","volume":"99","author":"D He","year":"2020","unstructured":"He D, Yang J, Jiang X, Lin Y, Chen H, Tang Y, Diao Y. A quantitative loop-mediated isothermal amplification assay for detecting a novel goose astrovirus. Poult Sci. 2020;99:6586\u201392. https:\/\/doi.org\/10.1016\/j.psj.2020.09.077.","journal-title":"Poult Sci"},{"key":"4131_CR39","doi-asserted-by":"publisher","first-page":"5875","DOI":"10.1093\/nar\/gky402","volume":"46","author":"J Kottur","year":"2018","unstructured":"Kottur J, Nair DT. Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction. Nucleic Acids Res. 2018;46:5875\u201385. https:\/\/doi.org\/10.1093\/nar\/gky402.","journal-title":"Nucleic Acids Res"},{"key":"4131_CR40","doi-asserted-by":"publisher","first-page":"20869","DOI":"10.1074\/jbc.R116.742494","volume":"291","author":"AK Vashishtha","year":"2016","unstructured":"Vashishtha AK, Wang J, Konigsberg WH. Different divalent cations alter the kinetics and fidelity of DNA polymerases *. J Biol Chem. 2016;291:20869\u201375. https:\/\/doi.org\/10.1074\/jbc.R116.742494.","journal-title":"J Biol Chem"},{"key":"4131_CR41","doi-asserted-by":"publisher","first-page":"125","DOI":"10.3934\/biophy.2018.2.125","volume":"5","author":"AK Vashishtha","year":"2018","unstructured":"Vashishtha AK, Konigsberg WH. The effect of different divalent cations on the kinetics and fidelity of Bacillus stearothermophilus DNA polymerase. AIMS Biophys. 2018;5:125\u201343. https:\/\/doi.org\/10.3934\/biophy.2018.2.125.","journal-title":"AIMS Biophys"},{"key":"4131_CR42","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1186\/s12936-019-3028-z","volume":"18","author":"J Cunningham","year":"2019","unstructured":"Cunningham J, Jones S, Gatton ML, Barnwell JW, Cheng Q, Chiodini PL, Glenn J, Incardona S, Kosack C, Luchavez J, Menard D, Nhem S, Oyibo W, Rees-Channer RR, Gonzalez I, Bell D. A review of the WHO malaria rapid diagnostic test product testing programme (2008\u20132018): performance, procurement and policy. Malar J. 2019;18:387. https:\/\/doi.org\/10.1186\/s12936-019-3028-z.","journal-title":"Malar J"},{"key":"4131_CR43","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1111\/jam.13647","volume":"124","author":"Y-P Wong","year":"2018","unstructured":"Wong Y-P, Othman S, Lau Y-L, Radu S, Chee H-Y. Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms. J Appl Microbiol. 2018;124:626\u201343. https:\/\/doi.org\/10.1111\/jam.13647.","journal-title":"J Appl Microbiol"},{"key":"4131_CR44","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1080\/14737159.2021.1873769","volume":"21","author":"TJ Moehling","year":"2021","unstructured":"Moehling TJ, Choi G, Dugan LC, Salit M, Meagher RJ. LAMP diagnostics at the point-of-care: emerging trends and perspectives for the developer community. Expert Rev Mol Diagn. 2021;21:43\u201361. https:\/\/doi.org\/10.1080\/14737159.2021.1873769.","journal-title":"Expert Rev Mol Diagn"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-022-04131-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-022-04131-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-022-04131-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T03:26:15Z","timestamp":1660274775000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-022-04131-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,3]]},"references-count":44,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["4131"],"URL":"https:\/\/doi.org\/10.1007\/s00216-022-04131-2","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,3]]},"assertion":[{"value":"4 April 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 May 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 May 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 June 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Initial testing of the LAMP received approval from the Human Biology Research Ethics Committee (HBREC) Cambridge, approval HBREC.2019.10: (\u201cSpecific, Sensitive and Rapid Detection of Plasmodium Infection in Malaria Patients and mosquitoes by Loop-mediated Isothermal Amplification (LAMP) and Recombinase Polymerase Amplification (RPA) assays\u201d).Ethical approval to test blood samples in Ghana was obtained for Malaria patients who were undergoing a trial on Artemisinin-Based Combination Therapy (\u201cThe Effects of Artemisinin-Based Combination Therapy (Act) on The Dynamics of Plasmodium Falciparum, P. Malariae and P. Ovale Infections in Ghana\u201d (GHS-ERC:005\/12\/17)). Patient samples were collected from Cape Coast (CC), Asikuma (ASK) and Baiden-Ghartey Hospital (BGH).Patient consent was obtained and the samples were anonymised providing only the regional testing location.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}