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However, the involvement of <jats:italic>N<\/jats:italic>-glycans in knee osteoarthritis (KOA) progression at the tissue level, especially within articular cartilage, is still poorly understood. Thus, the aim of this study was to spatially map and identify KOA-specific <jats:italic>N<\/jats:italic>-glycans from formalin-fixed paraffin-embedded (FFPE) osteochondral tissue of the tibial plateau relative to cadaveric control (CTL) tissues. Human FFPE osteochondral tissues from end-stage KOA patients (<jats:italic>n<\/jats:italic>=3) and CTL individuals (<jats:italic>n<\/jats:italic>=3), aged &gt;55 years old, were analyzed by matrix\u2010assisted laser desorption\/ionization mass spectrometry imaging (MALDI\u2010MSI) and liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS). Overall, it was revealed that 22 <jats:italic>N<\/jats:italic>-glycans were found in the cartilage region of KOA and CTL tissue. Of those, 15 <jats:italic>N<\/jats:italic>-glycans were more prominent in KOA cartilage than CTL cartilage. We then compared sub-regions of KOA and CTL tissues based on the Osteoarthritis Research Society International (OARSI) histopathological grade (1 to 6), where 1 is an intact cartilage surface and 6 is cartilage surface deformation. Interestingly, three specific complex-type <jats:italic>N<\/jats:italic>-glycans, (Hex)<jats:sub>4<\/jats:sub>(HexNAc)<jats:sub>3<\/jats:sub>, (Hex)<jats:sub>4<\/jats:sub>(HexNAc)<jats:sub>4<\/jats:sub>, and (Hex)<jats:sub>5<\/jats:sub>(HexNAc)<jats:sub>4<\/jats:sub>, were found to be localized to the superficial fibrillated zone of degraded cartilage (KOA OARSI 2.5-4), compared to adjacent cartilage with less degradation (KOA OARSI 1-2) or relatively healthy cartilage (CTL OARSI 1-2). Our results demonstrate that <jats:italic>N<\/jats:italic>-glycans specific to degraded cartilage in KOA patients have been identified at the tissue level for the first time. The presence of these <jats:italic>N<\/jats:italic>-glycans could further be evaluated as potential diagnostic and prognostic biomarkers.<\/jats:p>","DOI":"10.1007\/s00216-022-04289-9","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T07:02:36Z","timestamp":1663657356000},"page":"7597-7607","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mass spectrometry imaging spatially identifies complex-type N-glycans as putative cartilage degradation markers in human knee osteoarthritis tissue"],"prefix":"10.1007","volume":"414","author":[{"given":"Yea-Rin","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew T.","family":"Briggs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clifford","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R.","family":"Condina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia S.","family":"Kuliwaba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul H.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"issue":"1","key":"4289_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/nrrheum.2010.178","volume":"7","author":"DJ Hunter","year":"2011","unstructured":"Hunter DJ. 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