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For the first time, this bioreporter has been applied on the high-performance thin-layer chromatography (HPTLC) adsorbent surface to develop a respective planar bioluminescence bioassay (pA-YBS). The goal was to combine the advantages of HPTLC with a more selective bioassay detection for bisphenols. The performance of this pA-YBS bioluminescence bioassay was demonstrated by calculating the half-maximal effective concentration (EC<jats:sub>50<\/jats:sub>) of bisphenols compared to references. The EC<jats:sub>50<\/jats:sub> ranged from 267\u00a0pg\/band for bisphenol Z and 322\u00a0pg\/band for bisphenol A\u00a0(BPA) to\u2009&gt;\u20091\u00a0ng\/band for other bisphenols (BPC, BPE, BPF, and BPS) and references (17\u03b2-estradiol and 17\u03b1-ethinylestradiol). The EC<jats:sub>50<\/jats:sub> value of BPA was three times more sensitive in signal detection than that of 17\u03b2-estradiol. The visual or videodensitometric limit of detection of BPA was about 200\u00a0pg\/zone. The higher signal intensity and sensitivity for BPA confirmed the tailored bioassay selectivity compared to the existing estrogen screen bioassay. It worked on different types of HPTLC silica gel plates. This HPTLC\u2013UV\/Vis\/FLD\u2013pA-YBS bioluminescence bioassay method was used to analyze complex mixtures such as six tin can migrates, five thermal papers, and eleven botanicals. The detected estrogenic compound zones in the tin can migrates were successfully verified via the duplex planar yeast antagonist estrogen screen (pYAES) bioassay. The two bisphenols A and S were identified in one out of five thermal papers and confirmed with high-resolution mass spectrometry. No bisphenols were detected in the botanicals investigated via the pA-YBS bioluminescence bioassay. However, the botanicals proved to contain phytoestrogens as detected via the pYAES bioassay, which confirmed the tailored bioassay selectivity. This HPTLC\u2013UV\/Vis\/FLD\u2013pA-YBS bioluminescence bioassay is suited for cost-efficient analysis of BPA in complex samples, with no need for sterile conditions due to the fast workflow.<\/jats:p>","DOI":"10.1007\/s00216-023-04820-6","type":"journal-article","created":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T15:01:57Z","timestamp":1689606117000},"page":"5193-5204","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Screening bisphenols in complex samples via a planar Arxula adeninivorans bioluminescence bioassay"],"prefix":"10.1007","volume":"415","author":[{"given":"Max","family":"Jaber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"J\u00e4hne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Oberle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gertrud E.","family":"Morlock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,7,17]]},"reference":[{"key":"4820_CR1","unstructured":"Global bisphenol A market report 2018: analysis 2013\u20132017 & Forecasts 2018\u20132023. 2018. www.globenewswire.com\/en\/news-release\/2018\/11\/29\/1658800\/28124\/en\/Global-Bisphenol-A-Market-Report-2017-2023-Polycarbonates-Accounted-for-Around-64-05-of-the-Total-Consumption-in-Volume-Terms.html. Accessed 2 June 2023."},{"key":"4820_CR2","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2014.02.003","author":"Jaromir Micha\u0142owicz","year":"2014","unstructured":"Micha\u0142owicz J. Bisphenol A\u2013sources, toxicity and biotransformation. Environ Toxicol Pharmacol. 2014. https:\/\/doi.org\/10.1016\/j.etap.2014.02.003.","journal-title":"Environ Toxicol Pharmacol"},{"key":"4820_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2007.06.008","author":"RA Keri","year":"2007","unstructured":"Keri RA, Ho S-M, Hunt PA, Knudsen KE, Soto AM, Prins GS. An evaluation of evidence for the carcinogenic activity of bisphenol A. Reprod Toxicol. 2007. https:\/\/doi.org\/10.1016\/j.reprotox.2007.06.008.","journal-title":"Reprod Toxicol"},{"key":"4820_CR4","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7713","author":"FS vom Saal","year":"2005","unstructured":"vom Saal FS, Hughes C. An extensive new literature concerning low-dose effects of bisphenol A shows the need for a new risk assessment. Environ Health Perspect. 2005. https:\/\/doi.org\/10.1289\/ehp.7713.","journal-title":"Environ Health Perspect"},{"key":"4820_CR5","unstructured":"European Food Safety Authority. Bisphenol A in food is a health risk. 2023. www.efsa.europa.eu\/en\/news\/bisphenol-food-health-risk. 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Bisphenol A and its alternatives in Austrian thermal paper receipts, and the migration from reusable plastic drinking bottles into water and artificial saliva using UHPLC-MS\/MS. Chemosphere. 2022. https:\/\/doi.org\/10.1016\/j.chemosphere.2021.131842.","journal-title":"Chemosphere"},{"key":"4820_CR9","unstructured":"BPA being replaced by BPS in thermal paper, ECHA survey finds: results of a market survey conducted by ECHA show the volume of bisphenol S (BPS) used as developer in thermal paper manufactured in the EU doubled between 2016 and 2017. In: ECHA 2018, NR\/18\/31."},{"key":"4820_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.10.037","author":"G Gatidou","year":"2007","unstructured":"Gatidou G, Thomaidis NS, Stasinakis AS, Lekkas TD. Simultaneous determination of the endocrine disrupting compounds nonylphenol, nonylphenol ethoxylates, triclosan and bisphenol A in wastewater and sewage sludge by gas chromatography-mass spectrometry. 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