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Specifically, the presence of enniatins (A, A<jats:sub>1<\/jats:sub>, B, B<jats:sub>1<\/jats:sub>) and beauvericin has been monitored in brain, lung, kidney, fat, liver, and heart samples. A unique methodology based on solid\u2013liquid extraction (SLE) followed by dispersive liquid\u2013liquid microextraction (DLLME) was proposed for the six different matrices. Mycotoxin isolation was performed by adding ultrapure water, acetonitrile, and sodium chloride to the tissue sample for SLE, while the DLLME step was performed using chloroform as extraction solvent. Subsequently, the analysis was carried out by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC\u2013MS\/MS). The proposed method allowed limits of quantification (LOQs) to be obtained\u00a0in a range of 0.001\u20130.150 ng g<jats:sup>\u22121<\/jats:sup>, depending on the tissue and mycotoxin. The precision was investigated intraday and interday, not exceeding of 9.8% of relative standard deviation. In addition, trueness studies achieved 75 to 115% at a mycotoxin concentration of 25 ng g<jats:sup>\u22121<\/jats:sup> and from 82 to 118% at 5 ng g<jats:sup>\u22121<\/jats:sup>. The application of this methodology to 26 forensic autopsies demonstrated the bioaccumulation of emerging mycotoxins in the human body since all mycotoxins were detected in tissues. Enniatin B (ENNB) showed a high occurrence, being detected in 100% of liver (7\u2009\u00b1\u200913 ng g<jats:sup>\u22121<\/jats:sup>) and fat samples (0.2\u2009\u00b1\u20090.8 ng g<jats:sup>\u22121<\/jats:sup>). The\u00a0lung had a high incidence of all emerging mycotoxins at low concentrations, while ENNB, ENNB<jats:sub>1<\/jats:sub>, and ENNA<jats:sub>1<\/jats:sub> were not quantifiable in heart samples. Co-occurrence of mycotoxins was also investigated, and statistical tests were applied to evaluate the distribution of these mycotoxins in the human body.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00216-023-05040-8","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T06:04:34Z","timestamp":1700546674000},"page":"449-459","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of distribution of emerging mycotoxins in human tissues: applications of dispersive liquid\u2013liquid microextraction and liquid chromatography-mass spectrometry"],"prefix":"10.1007","volume":"416","author":[{"given":"Ana","family":"Castell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Arroyo-Manzanares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Palma-Manrique","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Campillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Fenoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5219-3711","authenticated-orcid":false,"given":"Pilar","family":"Vi\u00f1as","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"5040_CR1","doi-asserted-by":"publisher","first-page":"765","DOI":"10.3390\/toxins12120765","volume":"12","author":"M Urbaniak","year":"2020","unstructured":"Urbaniak M, Waskiewicz A, Stepien \u0141. 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