{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T03:45:01Z","timestamp":1740109501276,"version":"3.37.3"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007048","name":"EEA Grants","doi-asserted-by":"publisher","award":["PT-INNOVATION-0078 \u2013 COASTAL"],"award-info":[{"award-number":["PT-INNOVATION-0078 \u2013 COASTAL"]}],"id":[{"id":"10.13039\/501100007048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0094\/2020","SFRH\/BD\/120326\/2016","UIDB\/50017\/2020","UIDP\/50017\/2020"],"award-info":[{"award-number":["LA\/P\/0094\/2020","SFRH\/BD\/120326\/2016","UIDB\/50017\/2020","UIDP\/50017\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007689","name":"Universidade de Aveiro","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007689","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phytotoxins produced by marine microalgae, such as paralytic shellfish toxins (PSTs), can accumulate in bivalve molluscs, representing a human health concern due to the life-threatening symptoms they cause. To avoid the commercialization of contaminated bivalves, monitoring programs were established in the EU. The purpose of this work is the implementation of a PST transforming enzyme\u2014carbamoylase\u2014in an impedimetric test for rapid simultaneous detection of several carbamate and N-sulfocarbamoyl PSTs. Carbamoylase hydrolyses carbamate and sulfocarbamoyl toxins, which may account for up to 90% of bivalve toxicity related to PSTs. Conformational changes of carbamoylase accompanying enzymatic reactions were probed by Fourier transform mid-infrared spectroscopy (FT-MIR) and electrochemical impedance spectroscopy (EIS). Furthermore, a combination of EIS with a metal electrode and a carbamoylase-based assay was employed to harness changes in the enzyme conformation and adsorption on the electrode surface during the\u00a0enzymatic reaction as an analytical signal. After optimization of the working conditions, the developed impedimetric e-tongue could quantify N-sulfocarbamoyl toxins with a detection limit of 0.1 \u00b5M. The developed e-tongue allows the detection of these toxins at concentration levels observed in bivalves with PST toxicity close to the regulatory limit. The quantification of a sum of N-sulfocarbamoyl PSTs in naturally contaminated mussel extracts using the developed impedimetric e-tongue has been demonstrated.<\/jats:p>","DOI":"10.1007\/s00216-024-05199-8","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T14:02:12Z","timestamp":1708005732000},"page":"1983-1995","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Carbamoylase-based impedimetric electronic tongue for rapid detection of paralytic shellfish toxins"],"prefix":"10.1007","volume":"416","author":[{"given":"Mariana","family":"Raposo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Soreto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catarina","family":"Moreirinha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa S. R.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara T.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jo\u00e3o","family":"Botelho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno M. G.","family":"Melo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds Cadillon","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alisa","family":"Rudnitskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"5199_CR1","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.hal.2016.05.003","volume":"57","author":"LM Grattan","year":"2016","unstructured":"Grattan LM, Holobaugh S, Morris JG. Harmful algal blooms and public health. Harmful Algae. 2016;57:2\u20138. https:\/\/doi.org\/10.1016\/j.hal.2016.05.003.","journal-title":"Harmful Algae."},{"key":"5199_CR2","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1146\/annurev-marine-120308-081121","volume":"4","author":"DM Anderson","year":"2012","unstructured":"Anderson DM, Cembella AD, Hallegraeff GM. Progress in understanding harmful algal blooms: paradigm shifts and new technologies for research, monitoring, and management. Ann Rev Mar Sci. 2012;4:143\u201376. https:\/\/doi.org\/10.1146\/annurev-marine-120308-081121.","journal-title":"Ann Rev Mar Sci."},{"issue":"7","key":"5199_CR3","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.ocecoaman.2009.04.006","volume":"52","author":"DM Anderson","year":"2009","unstructured":"Anderson DM. Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean Coast Manag. 2009;52(7):342\u20137. https:\/\/doi.org\/10.1016\/j.ocecoaman.2009.04.006.","journal-title":"Ocean Coast Manag"},{"issue":"1","key":"5199_CR4","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.hal.2007.05.002","volume":"7","author":"P Vale","year":"2008","unstructured":"Vale P, Botelho MJ, Rodrigues SM, Gomes SS, Sampayo MAdeM. Two decades of marine biotoxin monitoring in bivalves from Portugal (1986\u20132006): a review of exposure assessment. Harmful Algae. 2008;7(1):11\u201325. https:\/\/doi.org\/10.1016\/j.hal.2007.05.002.","journal-title":"Harmful Algae."},{"key":"5199_CR5","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1039\/b501296c","volume":"23","author":"LE Llewellyn","year":"2006","unstructured":"Llewellyn LE. Saxitoxin, a toxic marine natural product that targets a multitude of receptors. Nat Prod Rep. 2006;23:200\u201322. https:\/\/doi.org\/10.1039\/b501296c.","journal-title":"Nat Prod Rep."},{"issue":"5","key":"5199_CR6","doi-asserted-by":"publisher","first-page":"344","DOI":"10.3390\/toxins12050344","volume":"12","author":"MIC Raposo","year":"2020","unstructured":"Raposo MIC, Gomes MTSR, Botelho MJ, Rudnitskaya A. Paralytic shellfish toxins (PST)-transforming enzymes: a review. Toxins. 2020;12(5):344. https:\/\/doi.org\/10.3390\/toxins12050344.","journal-title":"Toxins"},{"key":"5199_CR7","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1093\/jaoac\/88.6.1714","volume":"88","author":"AOAC International","year":"2005","unstructured":"AOAC International. AOAC Official Method 2005.06. Paralytic shellfish poisoning toxins in shellfish: prechromatographic oxidation and liquid chromatography with fluorescence detection. J AOAC Int. 2005;88:1714.","journal-title":"J AOAC Int."},{"issue":"7","key":"5199_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/s21072499","volume":"21","author":"M Dillon","year":"2021","unstructured":"Dillon M, Zaczek-Moczydlowska MA, Edwards C, Turner AD, Miller PI, Moore H, et al. Current trends and challenges for rapid smart diagnostics at point-of-site testing for marine toxins. Sensors. 2021;21(7):1\u201334. https:\/\/doi.org\/10.3390\/s21072499.","journal-title":"Sensors"},{"key":"5199_CR9","doi-asserted-by":"publisher","unstructured":"Camp\u00e0s M, Alkassar M, Gaiani G, Leonardo S, Rambla-Alegre M, Diog\u00e8ne J. The wide spectrum of methods available to study marine neurotoxins. In: Novelli A, Fern\u00e1ndez-S\u00e1nchez MF, Aschner M, Costa LG, editors. Advances in Neurotoxicology. Vol. 6. London: Elsevier; 2021. pp. 275\u2013315. https:\/\/doi.org\/10.1016\/bs.ant.2021.03.005","DOI":"10.1016\/bs.ant.2021.03.005"},{"key":"5199_CR10","unstructured":"Neogen. Reveal\u00ae 2.0 for PSP. 2022. https:\/\/www.neogen.com\/categories\/seafood-testing\/reveal-2-psp\/"},{"key":"5199_CR11","unstructured":"Saxitoxin Plate Kit, Beacon Analytical Systems Inc.; https:\/\/www.beaconkits.com\/product\/saxitoxin-plate-kit\/saxitoxin-plate-kit"},{"key":"5199_CR12","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.marenvres.2012.01.002","volume":"77","author":"MJ Botelho","year":"2012","unstructured":"Botelho MJ, Vale C, Grilo RV, Ferreira JG. Uptake and release of paralytic shellfish toxins by the clam Ruditapes decussatus exposed to Gymnodinium catenatum and subsequent depuration. Mar Environ Res. 2012;77:23\u20139. https:\/\/doi.org\/10.1016\/j.marenvres.2012.01.002.","journal-title":"Mar Environ Res."},{"key":"5199_CR13","unstructured":"Hallegraeff G, Anderson DM, Hole W, Cembella A. Manual on harmful marine microalgae. Paris: Unesco; 2003."},{"issue":"3","key":"5199_CR14","doi-asserted-by":"publisher","first-page":"795","DOI":"10.5740\/jaoacint.CS2011_27","volume":"95","author":"FM Van Dolah","year":"2012","unstructured":"Van Dolah FM, Fire SE, Leighfield TA, Mikulski CM, Doucette GJ. Determination of paralytic shellfish toxins in shellfish by receptor binding assay: collaborative study. J AOAC Int. 2012;95(3):795\u2013812. https:\/\/doi.org\/10.5740\/jaoacint.CS2011_27.","journal-title":"J AOAC Int"},{"issue":"11","key":"5199_CR15","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1080\/02652030110048594","volume":"18","author":"LE Llewellyn","year":"2001","unstructured":"Llewellyn LE, Doyle J, Jellett J, Barrett R, Alison C, Bentz C, et al. Measurement of paralytic shellfish toxins in molluscan extracts: comparison of the microtitre plate saxiphilin and sodium channel radioreceptor assays with mouse bioassay, HPLC analysis and a commercially available cell culture assay. Food Addit Contam. 2001;18(11):970\u201380. https:\/\/doi.org\/10.1080\/02652030110048594.","journal-title":"Food Addit Contam"},{"issue":"3","key":"5199_CR16","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1016\/j.talanta.2006.12.036","volume":"72","author":"M Camp\u00e0s","year":"2007","unstructured":"Camp\u00e0s M, Prieto-Sim\u00f3n B, Marty JL. Biosensors to detect marine toxins: assessing seafood safety. Talanta. 2007;72(3):884\u201395. https:\/\/doi.org\/10.1016\/j.talanta.2006.12.036.","journal-title":"Talanta"},{"issue":"181","key":"5199_CR17","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1016\/j.talanta.2018.01.031","volume":"1","author":"NS Ferreira","year":"2018","unstructured":"Ferreira NS, Cruz MGN, Gomes MTSR, Rudnitskaya A. Potentiometric chemical sensors for the detection of paralytic shellfish toxins. Talanta. 2018;1(181):380\u20134. https:\/\/doi.org\/10.1016\/j.talanta.2018.01.031.","journal-title":"Talanta"},{"key":"5199_CR18","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1351\/pac200577111965","volume":"77","author":"Y Vlasov","year":"2005","unstructured":"Vlasov Y, Legin A, Rudnitskaya A, Di Natale C, D\u2019Amico A. Nonspecific sensor arrays (\u201celectronic tongue\u201d) for chemical analysis of liquids: (IUPAC technical report). Pure Appl Chem. 2005;77:1965\u201383. https:\/\/doi.org\/10.1351\/pac200577111965.","journal-title":"Pure Appl Chem."},{"issue":"263","key":"5199_CR19","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1016\/j.snb.2018.02.158","volume":"15","author":"MGN Cruz","year":"2018","unstructured":"Cruz MGN, Ferreira NS, Gomes MTSR, Botelho MJ, Costa ST, Vale C, et al. Determination of paralytic shellfish toxins using potentiometric electronic tongue. Sens Actuators B Chem. 2018;15(263):550\u20136. https:\/\/doi.org\/10.1016\/j.snb.2018.02.158.","journal-title":"Sens Actuators B Chem"},{"issue":"2","key":"5199_CR20","doi-asserted-by":"publisher","first-page":"507","DOI":"10.3390\/s20020507","volume":"20","author":"M Raposo","year":"2020","unstructured":"Raposo M, Botelho MJ, Costa ST, Gomes MTSR, Rudnitskaya A. A carbamoylase-based bioassay for the detection of paralytic shellfish poisoning toxins. Sensors. 2020;20(2):507. https:\/\/doi.org\/10.3390\/s20020507.","journal-title":"Sensors."},{"key":"5199_CR21","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.hal.2005.05.005","volume":"5","author":"MD Fast","year":"2006","unstructured":"Fast MD, Cembella AD, Ross NW. In vitro transformation of paralytic shellfish toxins in the clams Mya arenaria and Protothaca staminea. Harmful Algae. 2006;5:79\u201390. https:\/\/doi.org\/10.1016\/j.hal.2005.05.005.","journal-title":"Harmful Algae."},{"key":"5199_CR22","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.toxicon.2013.01.008","volume":"65","author":"AD Turner","year":"2013","unstructured":"Turner AD, Lewis AM, O\u2019Neil A, Hatfield RG. Transformation of paralytic shellfish poisoning toxins in UK surf clams (Spisula solida) for targeted production of reference materials. Toxicon. 2013;65:41\u201358. https:\/\/doi.org\/10.1016\/j.toxicon.2013.01.008.","journal-title":"Toxicon."},{"key":"5199_CR23","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1007\/s00216-008-1970-7","volume":"391","author":"F Lisdat","year":"2008","unstructured":"Lisdat F, Sch\u00e4fer D. The use of electrochemical impedance spectroscopy for biosensing. Anal Bioanal Chem. 2008;391:1555\u201367. https:\/\/doi.org\/10.1007\/s00216-008-1970-7.","journal-title":"Anal Bioanal Chem."},{"issue":"11","key":"5199_CR24","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1002\/elan.200390114","volume":"15","author":"E Katz","year":"2003","unstructured":"Katz E, Willner I. Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedimetric immunosensors, DNA-sensors, and enzyme biosensors. Electroanal. 2003;15(11):913\u201347. https:\/\/doi.org\/10.1002\/elan.200390114.","journal-title":"Electroanal"},{"key":"5199_CR25","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/S0379-6779(02)00107-8","volume":"132","author":"A Riul","year":"2003","unstructured":"Riul A, Gallardo Soto AM, Mello SV, Bone S, Taylor DM, Mattoso LHC. An electronic tongue using polypyrrole and polyaniline. Synth Met. 2003;132:109\u201316. https:\/\/doi.org\/10.1016\/S0379-6779(02)00107-8.","journal-title":"Synth Met."},{"key":"5199_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.compag.2013.03.001","volume":"94","author":"PA Ulloa","year":"2013","unstructured":"Ulloa PA, Guerra R, Cavaco AM, Rosa da Costa AM, Figueira AC, Brigas AF. Determination of the botanical origin of honey by sensor fusion of impedance e-tongue and optical spectroscopy. Comput Electron Agric. 2013;94:1\u201311. https:\/\/doi.org\/10.1016\/j.compag.2013.03.001.","journal-title":"Comput Electron Agric."},{"key":"5199_CR27","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1016\/j.snb.2018.09.023","volume":"277","author":"C Garcia-Hernandez","year":"2018","unstructured":"Garcia-Hernandez C, Salvo Comino C, Mart\u00edn-Pedrosa F, Rodriguez-Mendez ML, Garcia-Cabezon C. Impedimetric electronic tongue based on nanocomposites for the analysis of red wines. Improving the variable selection method. Sens Actuators B Chem. 2018;277:365\u201372. https:\/\/doi.org\/10.1016\/j.snb.2018.09.023.","journal-title":"Sens Actuators B Chem."},{"issue":"11","key":"5199_CR28","doi-asserted-by":"publisher","first-page":"2470","DOI":"10.1016\/j.bios.2010.04.004","volume":"25","author":"JY Heras","year":"2010","unstructured":"Heras JY, Pallarola D, Battaglini F. Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin. Biosens Bioelectron. 2010;25(11):2470\u20136. https:\/\/doi.org\/10.1016\/j.bios.2010.04.004.","journal-title":"Biosens Bioelectron"},{"key":"5199_CR29","doi-asserted-by":"publisher","first-page":"139312","DOI":"10.1016\/j.electacta.2021.139312","volume":"397","author":"DR Rodrigues","year":"2021","unstructured":"Rodrigues DR, Fragoso WD, Lemos SG. Electronic tongue based on a single impedimetric sensor and complex numbers-supervised pattern recognition. Electrochim Acta. 2021;397:139312. https:\/\/doi.org\/10.1016\/j.electacta.2021.139312.","journal-title":"Electrochim Acta."},{"key":"5199_CR30","doi-asserted-by":"publisher","first-page":"104839","DOI":"10.1016\/j.marenvres.2019.104839","volume":"154","author":"MJ Botelho","year":"2020","unstructured":"Botelho MJ, Marques F, Freitas R, Pires A, Pereira E, Vale C. Paralytic shellfish toxin profiles in mussel, cockle and razor shell under post-bloom natural conditions: evidence of higher biotransformation in razor shells and cockles. Mar Environ Res. 2020;154:104839. https:\/\/doi.org\/10.1016\/j.marenvres.2019.104839.","journal-title":"Mar Environ Res."},{"issue":"9","key":"5199_CR31","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1016\/j.bbapap.2008.05.008","volume":"1784","author":"Y Cho","year":"2008","unstructured":"Cho Y, Ogawa N, Takahashi M, Lin HP, Oshima Y. Purification and characterization of paralytic shellfish toxin-transforming enzyme, sulfocarbamoylase I, from the Japanese bivalve Peronidia venulosa. Biochim Biophys Acta Proteins Proteom. 2008;1784(9):1277\u201385. https:\/\/doi.org\/10.1016\/j.bbapap.2008.05.008.","journal-title":"Biochim Biophys Acta Proteins Proteom"},{"issue":"1","key":"5199_CR32","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","volume":"150","author":"PK Smith","year":"1985","unstructured":"Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985;150(1):76\u201385. https:\/\/doi.org\/10.1016\/0003-2697(85)90442-7.","journal-title":"Anal Biochem"},{"issue":"6","key":"5199_CR33","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1093\/jaoac\/88.6.1714","volume":"88","author":"JF Lawrence","year":"2005","unstructured":"Lawrence JF, Niedzwiadek B, Menard C. Quantitative determination of paralytic shellfish poisoningtoxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study. J AOAC Int. 2005;88(6):1714\u201332. https:\/\/doi.org\/10.1093\/jaoac\/88.6.1714.","journal-title":"J AOAC Int"},{"issue":"12","key":"5199_CR34","doi-asserted-by":"publisher","first-page":"1724","DOI":"10.1080\/19440049.2010.525753","volume":"27","author":"MJ Botelho","year":"2010","unstructured":"Botelho MJ, Vale C, Mota AM, Rodrigues SM, Costa PR, Sim\u00f5esGon\u00e7alves MLS. Matrix effect on paralytic shellfish toxins quantification and toxicity estimation in mussels exposed to Gymnodinium catenatum. Food Addit Contam Part A. 2010;27(12):1724\u201332. https:\/\/doi.org\/10.1080\/19440049.2010.525753.","journal-title":"Food Addit Contam Part A."},{"key":"5199_CR35","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.chemosphere.2016.01.105","volume":"149","author":"ST Costa","year":"2016","unstructured":"Costa ST, Vale C, Raimundo J, Matias D, Botelho MJ. Changes of paralytic shellfish toxins in gills and digestive glands of the cockle Cerastoderma edule under post-bloom natural conditions. Chemosphere. 2016;149:351\u20137. https:\/\/doi.org\/10.1016\/j.chemosphere.2016.01.105.","journal-title":"Chemosphere."},{"key":"5199_CR36","first-page":"1","volume":"1019","author":"J Alexander","year":"2009","unstructured":"Alexander J, Benford D, Boobis A, Ceccatelli S, Cravedi JP, Di DA, Fern\u00e1ndez-Cruz ML, Fink-Gremmels J, F\u00fcrst P, Galli C, Grandjean P, Gzyl J, Heinemeyer G, Johansson N, Mutti A, Schlatter J, Van Leeuwen R, Van Peteghem C, Verger P. Scientific opinion of the panel on contaminants in the food chain on a request from the European Commission on Marine Biotoxins in Shellfish \u2013 Saxitoxin Group. The EFSA Journal. 2009;1019:1\u201376.","journal-title":"The EFSA Journal"},{"key":"5199_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-8-322","volume":"8","author":"DJ Vis","year":"2007","unstructured":"Vis DJ, Westerhuis JA, Smilde AK, van der Greef J. Statistical validation of megavariate effects in ASCA. BMC Bioinformatics. 2007;8:1\u20138. https:\/\/doi.org\/10.1186\/1471-2105-8-322.","journal-title":"BMC Bioinformatics."},{"issue":"9","key":"5199_CR38","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1002\/cem.952","volume":"19","author":"JJ Jansen","year":"2005","unstructured":"Jansen JJ, Hoefsloot HCJ, Van Der Greef J, Timmerman ME, Westerhuis JA, Smilde AK. ASCA: analysis of multivariate data obtained from an experimental design. J Chemom. 2005;19(9):469\u201381. https:\/\/doi.org\/10.1002\/cem.952.","journal-title":"J Chemom"},{"issue":"1\u20132","key":"5199_CR39","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S0169-7439(03)00110-2","volume":"69","author":"MC Ortiz","year":"2003","unstructured":"Ortiz MC, Sarabia LA, Herrero A, S\u00e1nchez MS, Sanz MB, Rueda ME, et al. Capability of detection of an analytical method evaluating false positive and false negative (ISO 11843) with partial least squares. Chemometr Intell Lab. 2003;69(1\u20132):21\u201333. https:\/\/doi.org\/10.1016\/S0169-7439(03)00110-2.","journal-title":"Chemometr Intell Lab"},{"issue":"9","key":"5199_CR40","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1016\/j.bbabio.2007.06.004","volume":"1767","author":"A Barth","year":"2007","unstructured":"Barth A. Infrared spectroscopy of proteins. Biochim Biophys Acta Bioenerg. 2007;1767(9):1073\u2013101. https:\/\/doi.org\/10.1016\/j.bbabio.2007.06.004.","journal-title":"Biochim Biophys Acta Bioenerg"},{"issue":"109","key":"5199_CR41","doi-asserted-by":"publisher","first-page":"107644","DOI":"10.1039\/c6ra20901g","volume":"6","author":"SV De Lima","year":"2016","unstructured":"De Lima SV, De Oliveira HP, De Melo CP. Electrical impedance monitoring of protein unfolding. RSC Adv. 2016;6(109):107644\u201352. https:\/\/doi.org\/10.1039\/c6ra20901g.","journal-title":"RSC Adv"},{"issue":"5","key":"5199_CR42","doi-asserted-by":"publisher","first-page":"1497","DOI":"10.3390\/molecules27051497","volume":"27","author":"CMA Brett","year":"2022","unstructured":"Brett CMA. Electrochemical impedance spectroscopy in the characterisation and application of modified electrodes for electrochemical sensors and biosensors. Molecules. 2022;27(5):1497. https:\/\/doi.org\/10.3390\/molecules27051497.","journal-title":"Molecules."},{"key":"5199_CR43","unstructured":"Brett CMA, Oliveira Brett AM. Electrochemistry: principles, methods, and applications. Oxford: Oxford University Press; 1993."},{"issue":"14\u201316","key":"5199_CR44","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1016\/j.ssi.2009.04.002","volume":"180","author":"MR Shoar Abouzari","year":"2009","unstructured":"Shoar Abouzari MR, Berkemeier F, Schmitz G, Wilmer D. On the physical interpretation of constant phase elements. Solid State Ion. 2009;180(14\u201316):922\u20137. https:\/\/doi.org\/10.1016\/j.ssi.2009.04.002.","journal-title":"Solid State Ion"},{"issue":"3","key":"5199_CR45","doi-asserted-by":"publisher","first-page":"318","DOI":"10.33961\/jecst.2020.00815","volume":"11","author":"BY Chang","year":"2020","unstructured":"Chang BY. Conversion of a constant phase element to an equivalent capacitor. J Electrochem Sci Technol. 2020;11(3):318\u201321. https:\/\/doi.org\/10.33961\/jecst.2020.00815.","journal-title":"J Electrochem Sci Technol."},{"key":"5199_CR46","doi-asserted-by":"publisher","unstructured":"Samet M, Levchenko V, Boiteux G, Seytre G, Kallel A, Serghei A. Electrode polarization vs. Maxwell-Wagner-Sillars interfacial polarization in dielectric spectra of materials: characteristic frequencies and scaling laws. J Chem Phys. 2015;142:194703. https:\/\/doi.org\/10.1063\/1.4919877","DOI":"10.1063\/1.4919877"},{"issue":"2","key":"5199_CR47","doi-asserted-by":"publisher","first-page":"69","DOI":"10.17628\/ecb.2020.9.69-74","volume":"9","author":"S Agiladevi","year":"2020","unstructured":"Agiladevi S, Rajendran S. Corrosion behaviour of 18 carat gold in ringer solutions in the presence of sodium chloride and glucose. Eur Chem Bull. 2020;9(2):69\u201374. https:\/\/doi.org\/10.17628\/ecb.2020.9.69-74.","journal-title":"Eur Chem Bull."},{"key":"5199_CR48","doi-asserted-by":"crossref","unstructured":"Botelho MJ, Vale C, Ferreira JG. Profiles of paralytic shellfish toxins in bivalves of low and elevated toxicities following exposure to Gymnodinium catenatum blooms in Portuguese estuarine and coastal waters. Chemosphere. 2015;138:1028\u201336. http:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653514015148.","DOI":"10.1016\/j.chemosphere.2014.12.072"},{"key":"5199_CR49","doi-asserted-by":"publisher","first-page":"108385","DOI":"10.1016\/j.foodhyd.2022.108385","volume":"137","author":"Y Weng","year":"2023","unstructured":"Weng Y, Ranaweera S, Zou D, Cameron AP, Chen X, Song H, et al. Improved enzyme thermal stability, loading and bioavailability using alginate encapsulation. Food Hydrocoll. 2023;137:108385. https:\/\/doi.org\/10.1016\/j.foodhyd.2022.108385.","journal-title":"Food Hydrocoll."},{"issue":"1","key":"5199_CR50","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.hal.2007.05.002","volume":"7","author":"P Vale","year":"2008","unstructured":"Vale P, Botelho MJ, Rodrigues SM, Gomes SS, Sampayo MAdeM. Two decades of marine biotoxin monitoring in bivalves from Portugal (1986\u20132006): a review of exposure assessment. Harmful Algae. 2008;7(1):11\u201325. https:\/\/doi.org\/10.1016\/j.hal.2007.05.002.","journal-title":"Harmful Algae."}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-024-05199-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-024-05199-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-024-05199-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T18:06:18Z","timestamp":1728410778000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-024-05199-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,15]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["5199"],"URL":"https:\/\/doi.org\/10.1007\/s00216-024-05199-8","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"type":"print","value":"1618-2642"},{"type":"electronic","value":"1618-2650"}],"subject":[],"published":{"date-parts":[[2024,2,15]]},"assertion":[{"value":"23 November 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 February 2024","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2024","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 February 2024","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}