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In cancer diagnostics, where biomarkers can lack specificity, multiparameter detection provides more information at the point-of-care. Our research focuses on epithelial ovarian cancer (EOC), where STn-glycosylated forms of CA125 and CA15-3 antigens can better discriminate cancer from benign conditions. We have developed a dual-label LFA that detects both CA125-STn and CA15-3-STn within a single anti-STn antibody test line. This utilizes spectral separation of green (540 nm) and blue (450 nm) emitting erbium (NaYF\n                    <jats:sub>4<\/jats:sub>\n                    :Yb\n                    <jats:sup>3+<\/jats:sup>\n                    , Er\n                    <jats:sup>3+<\/jats:sup>\n                    )- and thulium (NaYF\n                    <jats:sub>4<\/jats:sub>\n                    : Yb\n                    <jats:sup>3+<\/jats:sup>\n                    , Tm\n                    <jats:sup>3+<\/jats:sup>\n                    )-doped upconverting nanoparticle (UCNP) reporters conjugated with antibodies against the protein epitopes in CA125 or CA15-3. This technology allows the simultaneous detection of different antigen variants from a single test line. The developed proof-of-concept dual-label LFA was able to distinguish between the ascites fluid samples from diagnosed ovarian cancer patients (\n                    <jats:italic>n<\/jats:italic>\n                    = 10) and liver cirrhosis ascites fluid samples (\n                    <jats:italic>n<\/jats:italic>\n                    = 3) used as a negative control. The analytical sensitivity of CA125-STn for the dual-label LFA was 1.8 U\/ml in buffer and 3.6 U\/ml in ascites fluid matrix. Here we demonstrate a novel approach of spectrally separated measurement of STn-glycosylated forms of two different cancer-associated protein biomarkers by using UCNP reporter technology.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical Abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s00216-024-05275-z","type":"journal-article","created":{"date-parts":[[2024,4,7]],"date-time":"2024-04-07T22:05:37Z","timestamp":1712527537000},"page":"3251-3260","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3"],"prefix":"10.1007","volume":"416","author":[{"given":"Miikka","family":"Ekman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teppo","family":"Salminen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsti","family":"Raiko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tero","family":"Soukka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamlesh","family":"Gidwani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iida","family":"Martiskainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,8]]},"reference":[{"key":"5275_CR1","doi-asserted-by":"publisher","unstructured":"Martiskainen I, Talha SM, Vuorenp\u00e4\u00e4 K, Salminen T, Juntunen E, Chattopadhyay S, et al. 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