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The method was first developed in solution (linear response range from 2.4\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u00a0M to 1.0\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>\u00a0M). It was then implemented in cellulose platforms to develop a rapid test where the determination is made by measuring the RGB coordinates of the platforms using a smartphone-based device. The device is based on the integrating sphere concept and contains a light source to avoid external illumination effects. The smartphone is controlled by an app that allows a calibration line to be generated and the atropine concentration to be quantified; moreover, since the app normalizes the CCD response of the smartphone, the results and calibrations obtained with different smartphones are similar and can be shared. Using the G coordinate, the results were shown to have a linear response with the concentration of atropine ranging from 1.2\u2009\u00d7\u200910<jats:sup>\u22125<\/jats:sup>\u00a0M to 3.0\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>\u00a0M with an RSD of 1.4% (<jats:italic>n<\/jats:italic>\u2009=\u20095). The method has been applied to the determination of atropine in baby food and buckwheat samples with good results.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00216-024-05401-x","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T17:02:00Z","timestamp":1720026120000},"page":"7317-7323","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Colorimetric enzymatic rapid test for the determination of atropine in baby food using a smartphone"],"prefix":"10.1007","volume":"416","author":[{"given":"M.","family":"Dom\u00ednguez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Moraru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Lasso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Sanz-Vicente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7902-6005","authenticated-orcid":false,"given":"S.","family":"de Marcos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Galb\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"issue":"4","key":"5401_CR1","doi-asserted-by":"publisher","first-page":"245","DOI":"10.3390\/toxins15040245","volume":"15","author":"ARS Mateus","year":"2023","unstructured":"Mateus ARS, Crisafulli C, Vilhena M, Barros SC, Pena A, Sanches Silva A. 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