{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T12:33:11Z","timestamp":1745929991979,"version":"3.38.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["22374079"],"award-info":[{"award-number":["22374079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guangdong Pingheng Zhijian Biomedical Technology Ltd."}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1007\/s00216-025-05753-y","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T18:16:39Z","timestamp":1738001799000},"page":"1635-1647","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of insulin and bile acid complexes in liposome by different mass spectrometry techniques"],"prefix":"10.1007","volume":"417","author":[{"given":"Dandan","family":"Sha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minghui","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongli","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"issue":"12","key":"5753_CR1","doi-asserted-by":"publisher","first-page":"3085","DOI":"10.1007\/s00216-024-05258-0","volume":"416","author":"S Ma","year":"2024","unstructured":"Ma S, He R, Jiang T, Hu Z, Ye Z, Mi W. Development of an isotope dilution mass spectrometry assay for the quantification of insulin based on signature peptide analysis. Anal Bioanal Chem. 2024;416(12):3085\u201396. https:\/\/doi.org\/10.1007\/s00216-024-05258-0.","journal-title":"Anal Bioanal Chem"},{"issue":"4","key":"5753_CR2","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1021\/acs.molpharmaceut.0c01010","volume":"18","author":"S Ito","year":"2021","unstructured":"Ito S, Torii Y, Chikamatsu S, Harada T, Yamaguchi S, Ogata S, Sonoda K, Wakayama K, Masuda T, Ohtsuki S. Oral coadministration of Zn-insulin with d-form small intestine-permeable cyclic peptide enhances its blood glucose-lowering effect in mice. Mol Pharm. 2021;18(4):1593\u2013603. https:\/\/doi.org\/10.1021\/acs.molpharmaceut.0c01010.","journal-title":"Mol Pharm"},{"key":"5753_CR3","doi-asserted-by":"publisher","first-page":"133805","DOI":"10.1016\/j.ijbiomac.2024.133805","volume":"276","author":"Y Wang","year":"2024","unstructured":"Wang Y, Song W, Xue S, Sheng Y, Gao B, Dang Y, Zhang Y, Zhang GL. \u03b2-Cyclodextrin\/dialdehyde glucan-coated keratin nanoparticles for oral delivery of insulin. Int J Biol Macromol. 2024;276:133805. https:\/\/doi.org\/10.1016\/j.ijbiomac.2024.133805.","journal-title":"Int J Biol Macromol"},{"key":"5753_CR4","doi-asserted-by":"publisher","first-page":"4541","DOI":"10.1007\/s00216-019-01890-3","volume":"411","author":"M Dutta","year":"2019","unstructured":"Dutta M, Cai J, Gui W, Patterson AD. A review of analytical platforms for accurate bile acid measurement. Anal Bioanal Chem. 2019;411:4541\u20139. https:\/\/doi.org\/10.1007\/s00216-019-01890-3.","journal-title":"Anal Bioanal Chem"},{"issue":"1","key":"5753_CR5","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/s00216-023-05017-7","volume":"416","author":"T Gao","year":"2024","unstructured":"Gao T, Hu S, Xu W, Wang Z, Guo T, Chen F, Ma Y, Zhu L, Chen F, Wang X, Zhou J. Targeted LC-MS\/MS profiling of bile acids reveals primary\/secondary bile acid ratio as a novel biomarker for necrotizing enterocolitis. Anal Bioanal Chem. 2024;416(1):287\u201397. https:\/\/doi.org\/10.1007\/s00216-023-05017-7.","journal-title":"Anal Bioanal Chem"},{"key":"5753_CR6","doi-asserted-by":"publisher","unstructured":"Ge X, Huang S, Ren C, Zhao L. Taurocholic acid and glycocholic acid inhibit inflammation and activate farnesoid x receptor expression in LPS-stimulated zebrafish and macrophages. Molecules. 2023:28(5). https:\/\/doi.org\/10.3390\/molecules28052005.","DOI":"10.3390\/molecules28052005"},{"key":"5753_CR7","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.biomaterials.2017.09.022","volume":"147","author":"J Park","year":"2017","unstructured":"Park J, Choi J, Kim K, Byun Y. Bile acid transporter mediated endocytosis of oral bile acid conjugated nanocomplex. Biomaterials. 2017;147:145\u201354. https:\/\/doi.org\/10.1016\/j.biomaterials.2017.09.022.","journal-title":"Biomaterials"},{"issue":"2","key":"5753_CR8","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.ejpb.2012.02.009","volume":"81","author":"M Niu","year":"2012","unstructured":"Niu M, Lu Y, Hovgaard L, Guan P, Tan Y, Lian R, Qi J, Wu W. Hypoglycemic activity and oral bioavailability of insulin-loaded liposomes containing bile salts in rats: the effect of cholate type, particle size and administered dose. Eur J Pharm Biopharm. 2012;81(2):265\u201372. https:\/\/doi.org\/10.1016\/j.ejpb.2012.02.009.","journal-title":"Eur J Pharm Biopharm"},{"issue":"11","key":"5753_CR9","doi-asserted-by":"publisher","first-page":"3675","DOI":"10.1002\/fsn3.1224","volume":"7","author":"T Wang","year":"2019","unstructured":"Wang T, Shen L, Sun L, Zhang Y, Li H, Wang Y, Quan D. Why is glycocholic acid sodium salt better than deoxycholic acid sodium salt for the preparation of mixed micelle injections? Food Sci Nutr. 2019;7(11):3675\u201380. https:\/\/doi.org\/10.1002\/fsn3.1224.","journal-title":"Food Sci Nutr"},{"issue":"1","key":"5753_CR10","doi-asserted-by":"publisher","first-page":"6138438","DOI":"10.1155\/2020\/6138438","volume":"2020","author":"Y Wu","year":"2020","unstructured":"Wu Y, Zhou A, Tang L, Lei Y, Tang B, Zhang L. Bile acids: key regulators and novel treatment targets for type 2 diabetes. J Diabetes Res. 2020;2020(1):6138438. https:\/\/doi.org\/10.1155\/2020\/6138438.","journal-title":"J Diabetes Res"},{"issue":"2","key":"5753_CR11","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1515\/jtim-2016-0018","volume":"4","author":"GH Tomkin","year":"2016","unstructured":"Tomkin GH, Owens D. Obesity diabetes and the role of bile acids in metabolism. J Trans Intern Med. 2016;4(2):73\u201380. https:\/\/doi.org\/10.1515\/jtim-2016-0018.","journal-title":"J Trans Intern Med"},{"issue":"6","key":"5753_CR12","doi-asserted-by":"publisher","first-page":"2218","DOI":"10.1016\/j.gendis.2023.02.026","volume":"10","author":"J Wang","year":"2023","unstructured":"Wang J, Dai X, Chang H, Gao Q, Guo J, Yang JZ, Zhang S, Shan CL. Interruption of bile acid enterohepatic circulation inhibits glycogen synthesis and promotes hepatocellular carcinoma progression. Genes Dis. 2023;10(6):2218\u201321. https:\/\/doi.org\/10.1016\/j.gendis.2023.02.026.","journal-title":"Genes Dis"},{"issue":"10","key":"5753_CR13","doi-asserted-by":"publisher","first-page":"2198","DOI":"10.1021\/acs.jpcb.3c00274","volume":"127","author":"NA Jamuna","year":"2023","unstructured":"Jamuna NA, Kamalakshan A, Dandekar BR, Devassy AMC, Mondal J, Mandal S. Mechanistic insight into the amyloid fibrillation inhibition of hen egg white lysozyme by three different bile acids. J Phys Chem B. 2023;127(10):2198\u2013213. https:\/\/doi.org\/10.1021\/acs.jpcb.3c00274.","journal-title":"J Phys Chem B"},{"key":"5753_CR14","doi-asserted-by":"publisher","first-page":"124470","DOI":"10.1016\/j.ijbiomac.2023.124470","volume":"241","author":"D Roy","year":"2023","unstructured":"Roy D, Maity NC, Kumar S, Maity A, Ratha BN, Biswas R, Maiti RC, Mandal AK, Bhunia A. Modulatory role of copper on hIAPP aggregation and toxicity in presence of insulin. Int J Biol Macromol. 2023;241:124470. https:\/\/doi.org\/10.1016\/j.ijbiomac.2023.124470.","journal-title":"Int J Biol Macromol"},{"issue":"9","key":"5753_CR15","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1002\/psc.2423","volume":"18","author":"C Zhou","year":"2012","unstructured":"Zhou C, Xia X, Liu Y, Li L. The preparation of a complex of insulin-phospholipids and their interaction mechanism. J Pept Sci. 2012;18(9):541\u20138. https:\/\/doi.org\/10.1002\/psc.2423.","journal-title":"J Pept Sci"},{"issue":"1","key":"5753_CR16","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1021\/acs.bioconjchem.7b00593","volume":"29","author":"S Asayama","year":"2018","unstructured":"Asayama S, Nagashima K, Negishi Y, Kawakami H. Byproduct-free intact modification of insulin by cholesterol end-modified poly(ethylene glycol) for in vivo protein delivery. Bioconjugate Chem. 2018;29(1):67\u201373. https:\/\/doi.org\/10.1021\/acs.bioconjchem.7b00593.","journal-title":"Bioconjugate Chem"},{"issue":"23","key":"5753_CR17","doi-asserted-by":"publisher","first-page":"4148","DOI":"10.3390\/polym13234187","volume":"13","author":"H Xie","year":"2021","unstructured":"Xie H, Ma X, Lin W, Dong S, Liu Q, Chen Y, Gao Q. Linear dextrin as potential insulin delivery system: effect of degree of polymerization on the physicochemical properties of linear dextrin-insulin inclusion complexes. Polymers. 2021;13(23):4148. https:\/\/doi.org\/10.3390\/polym13234187.","journal-title":"Polymers"},{"issue":"1\u20132","key":"5753_CR18","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.aca.2010.10.032","volume":"686","author":"M Jing","year":"2011","unstructured":"Jing M, Bowser M. Methods for measuring aptamer-protein equilibria: a review. Anal Chim Acta. 2011;686(1\u20132):9\u201318. https:\/\/doi.org\/10.1016\/j.aca.2010.10.032.","journal-title":"Anal Chim Acta"},{"key":"5753_CR19","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1146\/anurev-biophys-102221-101121","volume":"51","author":"A Laganowsky","year":"2022","unstructured":"Laganowsky A, Clemmer DE, Russell DH. Variable-temperature native mass spectrometry for studies of protein folding, stabilities, assembly, and molecular interactions. Annu Rev Biophys. 2022;51:63\u201377. https:\/\/doi.org\/10.1146\/anurev-biophys-102221-101121.","journal-title":"Annu Rev Biophys"},{"issue":"20","key":"5753_CR20","doi-asserted-by":"publisher","first-page":"11523","DOI":"10.1002\/anie.202103931","volume":"60","author":"M Frick","year":"2021","unstructured":"Frick M, Schwieger C, Schmidt C. Cover picture: liposomes as carriers of membrane-associated proteins and peptides for mass spectrometric analysis. Angew Chem Int Ed. 2021;60(20):11523\u201330. https:\/\/doi.org\/10.1002\/anie.202103931.","journal-title":"Angew Chem Int Ed"},{"issue":"8","key":"5753_CR21","doi-asserted-by":"publisher","first-page":"7327","DOI":"10.1021\/acs.chemrev.1c00293","volume":"122","author":"JL Bennett","year":"2022","unstructured":"Bennett JL, Nguyen GTH, Donald WA. Protein-small molecule interactions in native mass spectrometry. Chem Rev. 2022;122(8):7327\u201385. https:\/\/doi.org\/10.1021\/acs.chemrev.1c00293.","journal-title":"Chem Rev"},{"key":"5753_CR22","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/j.tlanta.2013.05.061","volume":"116","author":"X Wang","year":"2013","unstructured":"Wang X, Liu Y, Wang H. A structure-differential binding method for elucidating the interactions between flavonoids and cytochrome-c by ESI-MS and molecular docking. Talanta. 2013;116:368\u201375. https:\/\/doi.org\/10.1016\/j.tlanta.2013.05.061.","journal-title":"Talanta"},{"issue":"13","key":"5753_CR23","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1002\/rcm.6248","volume":"26","author":"Y Du","year":"2012","unstructured":"Du Y, Zhang NB, Cui M, Liu ZQ, Liu SY. Studies of interaction between insulin and glutathione using electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom. 2012;26(13):1519\u201326. https:\/\/doi.org\/10.1002\/rcm.6248.","journal-title":"Rapid Commun Mass Spectrom"},{"issue":"5","key":"5753_CR24","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1016\/j.bbabio.2015.09.009","volume":"1857","author":"S Neya","year":"2016","unstructured":"Neya S, Nagai M, Nagatomo S, Hoshino T, Yoneda T, Kawaguchi AT. Utility of heme analogues to intentionally modify heme-globin interactions in myoglobin. BBA-Bioenergetics. 2016;1857(5):582\u20138. https:\/\/doi.org\/10.1016\/j.bbabio.2015.09.009.","journal-title":"BBA-Bioenergetics"},{"issue":"1","key":"5753_CR25","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1021\/acs.analchem.8b03829","volume":"91","author":"C Ren","year":"2019","unstructured":"Ren C, Bailey AO, VanderPorten E, Oh A, Phung W, Mulvihill MM, Harris SF, Liu YC, Han GH, Sandoval W. Quantitative determination of protein-ligand affinity by size exclusion chromatography directly coupled to high-resolution native mass spectrometry. Anal Chem. 2019;91(1):903\u201311. https:\/\/doi.org\/10.1021\/acs.analchem.8b03829.","journal-title":"Anal Chem"},{"issue":"6","key":"5753_CR26","doi-asserted-by":"publisher","first-page":"4292","DOI":"10.1021\/acs.analchem.9b04961","volume":"92","author":"IK Ventouri","year":"2020","unstructured":"Ventouri IK, Malheiro DBA, Voeten RLC, Kok S, Honing M, Somsen GW, Haselberg R. Probing protein denaturation during size-exclusion chromatography using native mass spectrometry. Anal Chem. 2020;92(6):4292\u2013300. https:\/\/doi.org\/10.1021\/acs.analchem.9b04961.","journal-title":"Anal Chem"},{"key":"5753_CR27","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1007\/s00216-019-01869-0","volume":"411","author":"X Zheng","year":"2019","unstructured":"Zheng X, Smith FB, Aly NA, et al. Evaluating the structural complexity of isomeric bile acids with ion mobility spectrometry. Anal Bioanal Chem. 2019;411:4673\u201382. https:\/\/doi.org\/10.1007\/s00216-019-01869-0.","journal-title":"Anal Bioanal Chem"},{"issue":"6","key":"5753_CR28","doi-asserted-by":"publisher","first-page":"2902","DOI":"10.1021\/acs.chemrev.2c00600","volume":"123","author":"E Christofi","year":"2023","unstructured":"Christofi E, Barran P. Ion mobility mass spectrometry (IM-MS) for structural biology: insights gained by measuring mass, charge, and collision cross section. Chem Rev. 2023;123(6):2902\u201349. https:\/\/doi.org\/10.1021\/acs.chemrev.2c00600.","journal-title":"Chem Rev"},{"key":"5753_CR29","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.ymeth.2015.05.017","volume":"95","author":"LM Young","year":"2016","unstructured":"Young LM, Saunders JC, Mahood RA, Revill CH, Foster RJ, Ashcroft AE, Radford SE. ESI-IMS-MS: a method for rapid analysis of protein aggregation and its inhibition by small molecules. Methods. 2016;95:62\u20139. https:\/\/doi.org\/10.1016\/j.ymeth.2015.05.017.","journal-title":"Methods"},{"issue":"27","key":"5753_CR30","doi-asserted-by":"publisher","first-page":"9397","DOI":"10.1021\/acs.anachem.1c00942","volume":"93","author":"TL Peterson","year":"2021","unstructured":"Peterson TL, Nagy G. Toward sequencing the human milk glycome: high-resolution cyclic ion mobility separations of core human milk oligosaccharide building blocks. Anal Chem. 2021;93(27):9397\u2013407. https:\/\/doi.org\/10.1021\/acs.anachem.1c00942.","journal-title":"Anal Chem"},{"issue":"36","key":"5753_CR31","doi-asserted-by":"publisher","first-page":"12435","DOI":"10.1021\/acs.anachem.2c02406","volume":"94","author":"JA Harrison","year":"2022","unstructured":"Harrison JA, Pruska A, Bittner P, Muck A, Cooper-Shepherd DA, Zenobi R. Advancing cyclic ion mobility mass spectrometry methods for studying biomolecules: toward the bonformational dynamics of mega dalton protein aggregates. Anal Chem. 2022;94(36):12435\u201343. https:\/\/doi.org\/10.1021\/acs.anachem.2c02406.","journal-title":"Anal Chem."},{"issue":"12","key":"5753_CR32","doi-asserted-by":"publisher","first-page":"7554","DOI":"10.1021\/acs.analchem.8b05641","volume":"91","author":"C Eldrid","year":"2019","unstructured":"Eldrid C, Ujma J, Kalfas S, Tomczyk N, Giles K, Morris M, Thalassinos K. Gas phase stability of protein ions in a cyclic ion mobility spectrometry traveling wave device. Anal Chem. 2019;91(12):7554\u201361. https:\/\/doi.org\/10.1021\/acs.analchem.8b05641.","journal-title":"Anal Chem"},{"key":"5753_CR33","doi-asserted-by":"publisher","first-page":"124142","DOI":"10.1016\/j.molliq.2024.124142","volume":"397","author":"SS Mohapatra","year":"2024","unstructured":"Mohapatra SS, Bisht KS, Dhar S, Biswas VK, Raghav SK, Kar RK, Maiti TK, Biswas A. Inhibition of amyloidal aggregation of insulin by amino acid conjugated bile acids: an insight into the possible role of biosurfactants in modulating the fibrillation kinetics and cytotoxicity. J Mol Liq. 2024;397:124142. https:\/\/doi.org\/10.1016\/j.molliq.2024.124142.","journal-title":"J Mol Liq"},{"issue":"17","key":"5753_CR34","doi-asserted-by":"publisher","first-page":"9483","DOI":"10.1021\/acs.analchem.7b02443","volume":"89","author":"C Qian","year":"2017","unstructured":"Qian C, Fu HQ, Kovalchik KA, Li HH, Chen DDY. Specific binding constant and stoichiometry determination in free solution by mass spectrometry and capillary electrophoresis frontal analysis. Anal Chem. 2017;89(17):9483\u201390. https:\/\/doi.org\/10.1021\/acs.analchem.7b02443.","journal-title":"Anal Chem"},{"issue":"6","key":"5753_CR35","doi-asserted-by":"publisher","first-page":"1530","DOI":"10.1021\/jasms.1c00130","volume":"32","author":"M G\u00fclfen","year":"2021","unstructured":"G\u00fclfen M, \u00d6zdemir A, Chen GH. Monitoring silver(I)-insulin complexes with electrospray ionization quadrupole ion trap mass spectrometry. J Am Soc Mass Spectrome. 2021;32(6):1530\u20137. https:\/\/doi.org\/10.1021\/jasms.1c00130.","journal-title":"J Am Soc Mass Spectrome"},{"key":"5753_CR36","doi-asserted-by":"publisher","first-page":"101529","DOI":"10.1016\/j.rineng.2023.101529","volume":"20","author":"S Kazhiyev","year":"2023","unstructured":"Kazhiyev S, Kaumbekova S, Shah D. Malic acid-based deep eutectic solvent and its application in insulin\u2019s structural stability. Results Eng. 2023;20:101529. https:\/\/doi.org\/10.1016\/j.rineng.2023.101529.","journal-title":"Results Eng"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-05753-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-025-05753-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-05753-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T17:07:38Z","timestamp":1741021658000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-025-05753-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,27]]},"references-count":36,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["5753"],"URL":"https:\/\/doi.org\/10.1007\/s00216-025-05753-y","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"type":"print","value":"1618-2642"},{"type":"electronic","value":"1618-2650"}],"subject":[],"published":{"date-parts":[[2025,1,27]]},"assertion":[{"value":"5 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 December 2024","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 January 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}