{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:49:42Z","timestamp":1791895782730,"version":"4.3.4"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No. 22274092"],"award-info":[{"award-number":["No. 22274092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1007\/s00216-025-05823-1","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T09:22:39Z","timestamp":1741598559000},"page":"2333-2343","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A dual-mode RNA-splitting aptamer biosensor for sensitive HIV Tat peptide detection via colorimetry and fluorescence"],"prefix":"10.1007","volume":"417","author":[{"given":"Rui","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Kang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze-Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Min","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Ying","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Qi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"issue":"8846","key":"5823_CR1","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1016\/0140-6736(93)90421-c","volume":"341","author":"MT Schechter","year":"1993","unstructured":"Schechter MT, Craib KJ, Gelmon KA, Montaner JS, Le TN, O\u2019Shaughnessy MV. HIV-1 and the aetiology of AIDS. Lancet. 1993;341(8846):658\u20139. https:\/\/doi.org\/10.1016\/0140-6736(93)90421-c.","journal-title":"Lancet."},{"issue":"23","key":"5823_CR2","doi-asserted-by":"publisher","first-page":"2376","DOI":"10.1001\/jama.2019.6919","volume":"321","author":"J Jin","year":"2019","unstructured":"Jin J. Who should be screened for HIV infection? JAMA. 2019;321(23):2376. https:\/\/doi.org\/10.1001\/jama.2019.6919.","journal-title":"JAMA."},{"issue":"1","key":"5823_CR3","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1097\/00126334-200201010-00010","volume":"29","author":"LI Gardner","year":"2002","unstructured":"Gardner LI, Holmberg SD, Moore J, Arnsten JH, Mayer KH, Rompalo A, et al. Use of highly active antiretroviral therapy in HIV-infected women: impact of HIV specialist care. J Acquir Immune Defic Syndr. 2002;29(1):69\u201375. https:\/\/doi.org\/10.1097\/00126334-200201010-00010.","journal-title":"J Acquir Immune Defic Syndr."},{"issue":"5","key":"5823_CR4","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1093\/jac\/dkq061","volume":"65","author":"S Moreno","year":"2010","unstructured":"Moreno S, Lopez Aldeguer J, Arribas JR, Domingo P, Iribarren JA, Ribera E, et al. The future of antiretroviral therapy: challenges and needs. J Antimicrob Chemother. 2010;65(5):827\u201335. https:\/\/doi.org\/10.1093\/jac\/dkq061.","journal-title":"J Antimicrob Chemother."},{"key":"5823_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113229","volume":"184","author":"Q Lu","year":"2021","unstructured":"Lu Q, Su T, Shang Z, Jin D, Shu Y, Xu Q, et al. Flexible paper-based Ni-MOF composite\/AuNPs\/CNTs film electrode for HIV DNA detection. Biosens Bioelectron. 2021;184: 113229. https:\/\/doi.org\/10.1016\/j.bios.2021.113229.","journal-title":"Biosens Bioelectron."},{"issue":"8543","key":"5823_CR6","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1016\/s0140-6736(87)92143-x","volume":"1","author":"W Borkowsky","year":"1987","unstructured":"Borkowsky W, Krasinski K, Paul D, Moore T, Bebenroth D, Chandwani S. Human-immunodeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay. Lancet. 1987;1(8543):1168\u201371. https:\/\/doi.org\/10.1016\/s0140-6736(87)92143-x.","journal-title":"Lancet."},{"issue":"6","key":"5823_CR7","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1038\/nbt0693-696","volume":"11","author":"E Papadopulos-Eleopulos","year":"1993","unstructured":"Papadopulos-Eleopulos E, Turner VF, Papadimitriou JM. Is a positive western blot proof of HIV infection? Nat Biotechnol. 1993;11(6):696\u2013707. https:\/\/doi.org\/10.1038\/nbt0693-696.","journal-title":"Nat Biotechnol."},{"key":"5823_CR8","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.338428","volume":"1159","author":"Z Li","year":"2021","unstructured":"Li Z, Xu H, Li S, Wu S, Miao X. Zettomole electrochemical HIV DNA detection using 2D DNA-Au nanowire structure, hemin\/G-quadruplex and polymerase chain reaction multi-signal synergistic amplification. Anal Chim Acta. 2021;1159: 338428. https:\/\/doi.org\/10.1016\/j.aca.2021.338428.","journal-title":"Anal Chim Acta."},{"key":"5823_CR9","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/1742-2094-10-113","volume":"10","author":"T Samikkannu","year":"2013","unstructured":"Samikkannu T, Rao KV, Arias AY, Kalaichezian A, Sagar V, Yoo C, et al. HIV infection and drugs of abuse: role of acute phase proteins. J Neuroinflammation. 2013;10:113. https:\/\/doi.org\/10.1186\/1742-2094-10-113.","journal-title":"J Neuroinflammation."},{"issue":"8","key":"5823_CR10","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1056\/NEJM198802253180803","volume":"318","author":"JW Ward","year":"1988","unstructured":"Ward JW, Holmberg SD, Allen JR, Cohn DL, Critchley SE, Kleinman SH, et al. Transmission of human immunodeficiency virus (HIV) by blood transfusions screened as negative for HIV antibody. N Engl J Med. 1988;318(8):473\u20138. https:\/\/doi.org\/10.1056\/NEJM198802253180803.","journal-title":"N Engl J Med."},{"key":"5823_CR11","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.bios.2015.11.067","volume":"78","author":"MF Fatin","year":"2015","unstructured":"Fatin MF, Ruslinda AR, Arshad MKM, Tee KK, Ayub RM, Hashim U, et al. HIV-1 Tat biosensor: current development and trends for early detection strategies. Biosens Bioelectron. 2015;78:358\u201366. https:\/\/doi.org\/10.1016\/j.bios.2015.11.067.","journal-title":"Biosens Bioelectron."},{"issue":"20","key":"5823_CR12","doi-asserted-by":"publisher","first-page":"4042","DOI":"10.1021\/jm980728e","volume":"42","author":"V Peytou","year":"1999","unstructured":"Peytou V, Condom R, Patino N, Guedj R, Aubertin AM, Gelus N, et al. Synthesis and antiviral activity of ethidium-arginine conjugates directed against the TAR RNA of HIV-1. J Med Chem. 1999;42(20):4042\u201353. https:\/\/doi.org\/10.1021\/jm980728e.","journal-title":"J Med Chem."},{"issue":"8","key":"5823_CR13","doi-asserted-by":"publisher","first-page":"2376","DOI":"10.1021\/ja044885g","volume":"127","author":"L Zeng","year":"2005","unstructured":"Zeng L, Li J, Muller M, Yan S, Mujtaba S, Pan C, et al. Selective small molecules blocking HIV-1 Tat and coactivator PCAF association. J Am Chem Soc. 2005;127(8):2376\u20137. https:\/\/doi.org\/10.1021\/ja044885g.","journal-title":"J Am Chem Soc."},{"issue":"11","key":"5823_CR14","doi-asserted-by":"publisher","first-page":"4695","DOI":"10.1021\/acs.analchem.1c04889","volume":"94","author":"J Li","year":"2022","unstructured":"Li J, Fan Y-Y, Wen J, Zhang J, Zhang ZQ. Metal-enhanced aggregation-induced emission strategy for the HIV-I RNA-binding ligand assay. Anal Chem. 2022;94(11):4695\u2013702. https:\/\/doi.org\/10.1021\/acs.analchem.1c04889.","journal-title":"Anal Chem."},{"issue":"19","key":"5823_CR15","doi-asserted-by":"publisher","first-page":"13532","DOI":"10.1021\/acs.analchem.0c03010","volume":"92","author":"J Li","year":"2020","unstructured":"Li J, Fan Y-Y, Wang M, Duan HL, Zhang J, Dang FQ, et al. A light-up strategy with aggregation-induced emission for identification of HIV-I RNA-binding small molecules. Anal Chem. 2020;92(19):13532\u20138. https:\/\/doi.org\/10.1021\/acs.analchem.0c03010.","journal-title":"Anal Chem."},{"issue":"22","key":"5823_CR16","doi-asserted-by":"publisher","first-page":"3512","DOI":"10.1101\/gad.12.22.3512","volume":"12","author":"ME Garber","year":"1998","unstructured":"Garber ME, Wei P, KewalRamani VN, Mayall TP, Herrmann CH, Rice AP, et al. The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein. Genes Dev. 1998;12(22):3512\u201327. https:\/\/doi.org\/10.1101\/gad.12.22.3512.","journal-title":"Genes Dev."},{"key":"5823_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1146\/annurev.biochem.67.1.1","volume":"67","author":"AD Frankel","year":"1998","unstructured":"Frankel AD, Young JAT. HIV-1: fifteen proteins and an RNA. Annu Rev Biochem. 1998;67:1\u201325. https:\/\/doi.org\/10.1146\/annurev.biochem.67.1.1.","journal-title":"Annu Rev Biochem."},{"issue":"13","key":"5823_CR18","doi-asserted-by":"publisher","first-page":"8622","DOI":"10.1021\/acs.analchem.9b02040","volume":"91","author":"S Wang","year":"2019","unstructured":"Wang S, Song W, Wei S, Zeng S, Yang S, Lei C, et al. Functional titanium carbide MXenes-loaded entropy-driven RNA explorer for long noncoding RNA PCA3 imaging in live cells. Anal Chem. 2019;91(13):8622\u20139. https:\/\/doi.org\/10.1021\/acs.analchem.9b02040.","journal-title":"Anal Chem."},{"key":"5823_CR19","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.jtbi.2019.04.018","volume":"473","author":"LU Aguilera","year":"2019","unstructured":"Aguilera LU, Rodriguez-Gonzalez J. Modeling the effect of tat inhibitors on HIV latency. J Theor Biol. 2019;473:20\u20137. https:\/\/doi.org\/10.1016\/j.jtbi.2019.04.018.","journal-title":"J Theor Biol."},{"key":"5823_CR20","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.jconrel.2021.05.016","volume":"335","author":"J Li","year":"2021","unstructured":"Li J, Wei J, Wan Y, Du X, Bai X, Li C, et al. TAT-modified tetramethylpyrazine-loaded nanoparticles for targeted treatment of spinal cord injury. J Control Release. 2021;335:103\u201316. https:\/\/doi.org\/10.1016\/j.jconrel.2021.05.016.","journal-title":"J Control Release."},{"issue":"35","key":"5823_CR21","doi-asserted-by":"publisher","first-page":"24654","DOI":"10.1021\/jacs.4c08824","volume":"146","author":"J Li","year":"2024","unstructured":"Li J, Yao P, Tang K, Zhao X, Liu X, Liu Q, et al. Functional aptamers in vitro evolution for intranuclear blockage of RNA-protein interaction. J Am Chem Soc. 2024;146(35):24654\u201362. https:\/\/doi.org\/10.1021\/jacs.4c08824.","journal-title":"J Am Chem Soc."},{"issue":"11","key":"5823_CR22","doi-asserted-by":"publisher","first-page":"922","DOI":"10.3390\/bios12110922","volume":"12","author":"Z Gan","year":"2022","unstructured":"Gan Z, Roslan MAM, Abd Shukor MY, Halim M, Yasid NA, Abdullah J, et al. Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application. Biosensors. 2022;12(11):922. https:\/\/doi.org\/10.3390\/bios12110922.","journal-title":"Biosensors."},{"issue":"6","key":"5823_CR23","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1089\/nat.2017.0680","volume":"27","author":"C Kratschmer","year":"2017","unstructured":"Kratschmer C, Levy M. Effect of chemical modifications on aptamer stability in serum. Nucleic Acid Ther. 2017;27(6):335\u201344. https:\/\/doi.org\/10.1089\/nat.2017.0680.","journal-title":"Nucleic Acid Ther."},{"issue":"27","key":"5823_CR24","doi-asserted-by":"publisher","first-page":"4126","DOI":"10.2174\/092986711797189565","volume":"18","author":"RE Wang","year":"2011","unstructured":"Wang RE, Wu H, Niu Y, Cai J. Improving the stability of aptamers by chemical modification. Curr Med Chem. 2011;18(27):4126\u201338. https:\/\/doi.org\/10.2174\/092986711797189565.","journal-title":"Curr Med Chem."},{"issue":"22","key":"5823_CR25","doi-asserted-by":"publisher","first-page":"13708","DOI":"10.1021\/acs.analchem.8b04107","volume":"90","author":"Y-Y Fan","year":"2018","unstructured":"Fan Y-Y, Mou ZL, Wang M, Li J, Zhang J, Dang FQ, et al. Chimeric aptamers-based and MoS2 nanosheet-enhanced label-free fluorescence polarization strategy for adenosine triphosphate detection. Anal Chem. 2018;90(22):13708\u201313. https:\/\/doi.org\/10.1021\/acs.analchem.8b04107.","journal-title":"Anal Chem."},{"key":"5823_CR26","doi-asserted-by":"publisher","first-page":"130561","DOI":"10.1016\/j.snb.2021.130561","volume":"346","author":"Y-Y Fan","year":"2021","unstructured":"Fan Y-Y, Li J, Fan L, Wen J, Zhang J, Zhang ZQ. A label-free aptasensor based on a dual-emission fluorescent strategy for aflatoxin B1 detection. Sens Actuators B Chem. 2021;346:130561. https:\/\/doi.org\/10.1016\/j.snb.2021.130561.","journal-title":"Sens Actuators B Chem."},{"key":"5823_CR27","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1093\/nass\/42.1.269","volume":"42","author":"M Katahira","year":"1999","unstructured":"Katahira M, Kobayashi S, Matsugami A, Ouhashi K, Uesugi S, Yamamoto R, et al. Structural study of an RNA aptamer for a Tat protein complexed with ligands. Nucleic Acids Symp Ser. 1999;42:269\u201370. https:\/\/doi.org\/10.1093\/nass\/42.1.269.","journal-title":"Nucleic Acids Symp Ser."},{"issue":"6","key":"5823_CR28","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1016\/j.bios.2004.03.037","volume":"20","author":"M Minunni","year":"2004","unstructured":"Minunni M, Tombelli S, Gullotto A, Luzi E, Mascini M. Development of biosensors with aptamers as bio-recognition element: the case of HIV-1 Tat protein. Biosens Bioelectron. 2004;20(6):1149\u201356. https:\/\/doi.org\/10.1016\/j.bios.2004.03.037.","journal-title":"Biosens Bioelectron."},{"issue":"2","key":"5823_CR29","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.bioelechem.2004.04.011","volume":"67","author":"S Tombelli","year":"2005","unstructured":"Tombelli S, Minunni M, Luzi E, Mascini M. Aptamer-based biosensors for the detection of HIV-1 Tat protein. Bioelectrochemistry. 2005;67(2):135\u201341. https:\/\/doi.org\/10.1016\/j.bioelechem.2004.04.011.","journal-title":"Bioelectrochemistry."},{"issue":"12","key":"5823_CR30","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1063\/1.3643067","volume":"99","author":"A Rahim Ruslinda","year":"2011","unstructured":"Rahim Ruslinda A, Wang X, Ishii Y, Ishiyama Y, Tanabe K, Kawarada H. Human immunodeficiency virus trans-activator of transcription peptide detection via ribonucleic acid aptamer on aminated diamond biosensor. Appl Phys Lett. 2011;99(12):253. https:\/\/doi.org\/10.1063\/1.3643067.","journal-title":"Appl Phys Lett."},{"issue":"1","key":"5823_CR31","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.bios.2012.07.048","volume":"40","author":"A Rahim Ruslinda","year":"2013","unstructured":"Rahim Ruslinda A, Tanabe K, Ibori S, Wang X, Kawarada H. Effects of diamond-FET-based RNA aptamer sensing for detection of real sample of HIV-1 Tat protein. Biosens Bioelectron. 2013;40(1):277\u201382. https:\/\/doi.org\/10.1016\/j.bios.2012.07.048.","journal-title":"Biosens Bioelectron."},{"key":"5823_CR32","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.procbio.2018.12.016","volume":"79","author":"MF Fatin","year":"2019","unstructured":"Fatin MF, Rahim Ruslinda A, Gopinath SCB, Arshad MKM, Hashim U, Lakshmipriya T, et al. Co-ordinated split aptamer assembly and disassembly on gold nanoparticle for functional detection of HIV-1 tat. Process Biochem. 2019;79:32\u20139. https:\/\/doi.org\/10.1016\/j.procbio.2018.12.016.","journal-title":"Process Biochem."},{"key":"5823_CR33","doi-asserted-by":"publisher","first-page":"132437","DOI":"10.1016\/j.snb.2022.132437","volume":"370","author":"FJ Lei","year":"2022","unstructured":"Lei FJ, Ye ZY, Dong Z, Zhang XF, Wu P. Thioflavine T-induced charge neutralization assembly of AuNPs for colorimetric sensing of thallium. Sens Actuators B Chem. 2022;370:132437. https:\/\/doi.org\/10.1016\/j.snb.2022.132437.","journal-title":"Sens Actuators B Chem."},{"issue":"2","key":"5823_CR34","doi-asserted-by":"publisher","first-page":"293","DOI":"10.3390\/bios13020293","volume":"13","author":"H Tang","year":"2023","unstructured":"Tang H, Peng J, Jiang X, Peng S, Wang F, Weng X, et al. A CRISPR-Cas and Tat peptide with fluorescent RNA aptamer system for signal amplification in RNA imaging. Biosensors. 2023;13(2):293. https:\/\/doi.org\/10.3390\/bios13020293.","journal-title":"Biosensors."},{"key":"5823_CR35","doi-asserted-by":"publisher","first-page":"134731","DOI":"10.1016\/j.snb.2023.134731","volume":"398","author":"F Lei","year":"2024","unstructured":"Lei F, Li X, Yang B, Ying B, Wu P. Visual monitoring of lead exposure in children\u2019s daily activities with an AuNP-based indicator. Sens Actuators B Chem. 2024;398:134731. https:\/\/doi.org\/10.1016\/j.snb.2023.134731.","journal-title":"Sens Actuators B Chem."},{"key":"5823_CR36","doi-asserted-by":"publisher","first-page":"117755","DOI":"10.1016\/j.trac.2024.117755","volume":"177","author":"LH Zhao","year":"2024","unstructured":"Zhao LH, Wu DD, Xiao SQ, Yin YG, Li L, Wang JC, et al. Dual-mode aptasensors with cross validation capacity for reliability enhancement and analytical assurance. Trends Anal Chem. 2024;177:117755. https:\/\/doi.org\/10.1016\/j.trac.2024.117755.","journal-title":"Trends Anal Chem."},{"issue":"10","key":"5823_CR37","doi-asserted-by":"publisher","first-page":"893","DOI":"10.3390\/bios12100893","volume":"12","author":"WY Zhang","year":"2022","unstructured":"Zhang WY, Tian T, Peng LJ, Zhou HY, Zhang H, Chen H, et al. A paper-based analytical device integrated with smartphone: fluorescent and colorimetric dual-mode detection of beta-glucosidase activity. Biosensors. 2022;12(10):893. https:\/\/doi.org\/10.3390\/bios12100893.","journal-title":"Biosensors."},{"key":"5823_CR38","doi-asserted-by":"publisher","first-page":"115553","DOI":"10.1016\/j.bios.2023.115553","volume":"237","author":"Y Wang","year":"2023","unstructured":"Wang Y, Xianyu Y. Tuning the plasmonic and catalytic signals of Au@Pt nanoparticles for dual-mode biosensing. Biosens Bioelectron. 2023;237:115553. https:\/\/doi.org\/10.1016\/j.bios.2023.115553.","journal-title":"Biosens Bioelectron."},{"key":"5823_CR39","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/j.bios.2016.02.001","volume":"80","author":"W Hu","year":"2016","unstructured":"Hu W, Chen Q, Li H, Ouyang Q, Zhao J. Fabricating a novel label-free aptasensor for acetamiprid by fluorescence resonance energy transfer between NH2-NaYF4: Yb, Ho@SiO2 and Au nanoparticles. Biosens Bioelectron. 2016;80:398\u2013404. https:\/\/doi.org\/10.1016\/j.bios.2016.02.001.","journal-title":"Biosens Bioelectron."},{"issue":"41","key":"5823_CR40","doi-asserted-by":"publisher","first-page":"13886","DOI":"10.1021\/acs.analchem.1c02736","volume":"93","author":"Y Zha","year":"2021","unstructured":"Zha Y, Li Y, Hu P, Lu S, Ren H, Liu Z, et al. Duplex-specific nuclease-triggered fluorescence immunoassay based on dual-functionalized AuNP for acetochlor, metolachlor, and propisochlor. Anal Chem. 2021;93(41):13886\u201392. https:\/\/doi.org\/10.1021\/acs.analchem.1c02736.","journal-title":"Anal Chem."},{"issue":"7","key":"5823_CR41","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1007\/s00216-003-2058-z","volume":"376","author":"W Dormeyer","year":"2003","unstructured":"Dormeyer W, Dorr A, Ott M, Schn\u00f6lzer M. Acetylation of the HIV-1 Tat protein: an in vitro study. Anal Bioanal Chem. 2003;376(7):994\u20131005. https:\/\/doi.org\/10.1007\/s00216-003-2058-z.","journal-title":"Anal Bioanal Chem."},{"issue":"6","key":"5823_CR42","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.1111\/cbdd.13706","volume":"96","author":"JBV Chiongson","year":"2020","unstructured":"Chiongson JBV, Sabido EM, Lin K-G, Alea GV, Dalisay DS, Wu S-H, et al. Multimeric TAT peptides are effective in vitro inhibitors of Staphylococcus saprophyticus. Chem Bio Drug Des. 2020;96(6):1348\u201354. https:\/\/doi.org\/10.1111\/cbdd.13706.","journal-title":"Chem Bio Drug Des."},{"issue":"1","key":"5823_CR43","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/S1386-6532(00)00189-X","volume":"21","author":"MC Re","year":"2001","unstructured":"Re MC, Vignoli M, Furlini G, Gibellini D, Colangeli V, Vitone F, et al. Antibodies against full-length Tat protein and some low-molecular-weight Tat-peptides correlate with low or undetectable viral load in HIV-1 seropositive patients. J Clin Virol. 2001;21(1):81\u20139. https:\/\/doi.org\/10.1016\/S1386-6532(00)00189-X.","journal-title":"J Clin Virol."},{"issue":"1","key":"5823_CR44","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.ymeth.2010.07.001","volume":"53","author":"S Pagans","year":"2011","unstructured":"Pagans S, Sakane N, Schn\u00f6lzer M, Ott M. Characterization of HIV Tat modifications using novel methyl-lysine-specific antibodies. Methods. 2011;53(1):91\u20136. https:\/\/doi.org\/10.1016\/j.ymeth.2010.07.001.","journal-title":"Methods."}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-05823-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-025-05823-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-05823-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T08:35:25Z","timestamp":1744619725000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-025-05823-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,10]]},"references-count":44,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["5823"],"URL":"https:\/\/doi.org\/10.1007\/s00216-025-05823-1","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,10]]},"assertion":[{"value":"19 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 January 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 February 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 March 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"There were no humans or animals were involved in this study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}