{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:01:21Z","timestamp":1792717281718,"version":"4.5.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"16","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["Collaborative pairs grant DAF2022-250616"],"award-info":[{"award-number":["Collaborative pairs grant DAF2022-250616"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Interest in the role of bis(monoacylglycero)phosphate (BMP) lipids in lysosomal function has significantly grown in recent years. Emerging evidence highlights BMPs as critical players not only in Niemann-Pick disease type C (NPC) but also in other pathologies such as neurodegeneration, cardiovascular diseases, and cancers. However, the selective analysis of BMPs is significantly hindered by isomeric phosphatidylglycerol (PG) lipids. While this can be addressed by chromatographic separation, it poses a significant challenge for shotgun lipidomics approaches. Here, we present a shotgun lipidomics strategy to detect and separate BMPs from PGs using differential fragmentation of sodiated ions. This approach, including isotope correction, is integrated into an existing quantitative shotgun lipidomics workflow (Lipidyzer combined with Shotgun Lipidomics Assistant software) that simultaneously quantifies\u2009&gt;1400 lipids. Validation using K-562 cell extracts demonstrated acceptable linearity, trueness, repeatability, and a limit of quantification of 0.12 \u00b5M, confirming robust analytical performance. Finally, characteristic accumulation of BMP lipids is shown in bone marrow\u2013derived macrophages from NPC mice, demonstrating its applicability. Our method presents a quantitative, selective, rapid, and robust solution for shotgun-based BMP analysis without the need for extensive chromatographic separation or derivatization. The integration of BMP lipid detection into the Lipidyzer platform, alongside the recently launched iSODA data visualization tool, empowers chemists and biologists to gain deeper insights into BMP lipid biology.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical Abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s00216-025-05890-4","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T01:47:35Z","timestamp":1746755255000},"page":"3665-3673","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Label-free quantitative shotgun analysis of bis(monoacylglycero)phosphate lipids"],"prefix":"10.1007","volume":"417","author":[{"given":"Lian Y.","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rico J. 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Direct infusion-tandem mass spectrometry (DI-MS\/MS) analysis of complex lipids in human plasma and serum using the Lipidyzer\u2122 platform. Methods Mol Biol (Clifton, NJ). 2018;1730:227\u201336.","journal-title":"Methods Mol Biol (Clifton, NJ)"},{"issue":"1","key":"5890_CR2","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1146\/annurev-biochem-092823-113814","volume":"93","author":"UN Medoh","year":"2024","unstructured":"Medoh UN, Abu-Remaileh M. The bis(monoacylglycero)-phosphate hypothesis: from lysosomal function to therapeutic avenues. Annu Rev Biochem. 2024;93(1):447\u201369.","journal-title":"Annu Rev Biochem"},{"issue":"18","key":"5890_CR3","doi-asserted-by":"publisher","first-page":"4651","DOI":"10.1016\/j.cell.2021.08.002","volume":"184","author":"T Logan","year":"2021","unstructured":"Logan T, Simon MJ, Rana A, Cherf GM, Srivastava A, Davis SS, et al. Rescue of a lysosomal storage disorder caused by Grn loss of function with a brain penetrant progranulin biologic. Cell. 2021;184(18):4651-68.e25.","journal-title":"Cell"},{"issue":"5","key":"5890_CR4","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1194\/jlr.M057851","volume":"56","author":"Z Akgoc","year":"2015","unstructured":"Akgoc Z, Sena-Esteves M, Martin DR, Han X, d\u2019Azzo A, Seyfried TN. Bis(monoacylglycero)phosphate: a secondary storage lipid in the gangliosidoses. J Lipid Res. 2015;56(5):1006\u201313.","journal-title":"J Lipid Res"},{"issue":"16","key":"5890_CR5","doi-asserted-by":"publisher","first-page":"8490","DOI":"10.1021\/acs.analchem.7b02058","volume":"89","author":"M Wang","year":"2017","unstructured":"Wang M, Palavicini JP, Cseresznye A, Han X. Strategy for quantitative analysis of isomeric bis(monoacylglycero)phosphate and phosphatidylglycerol species by shotgun lipidomics after one-step methylation. 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A DMS shotgun lipidomics workflow application to facilitate high-throughput, comprehensive lipidomics. J Am Soc Mass Spectrom. 2021;32(11):2655\u201363.","journal-title":"J Am Soc Mass Spectrom"},{"key":"5890_CR9","doi-asserted-by":"crossref","unstructured":"Ghorasaini M, Mohammed Y, Adamski J, Bettcher L, Bowden JA, Cabruja M, et al. Cross-laboratory standardization of preclinical lipidomics using differential mobility spectrometry and multiple reaction monitoring. Anal Chem. 2021;93(49):16369\u201378.","DOI":"10.1021\/acs.analchem.1c02826"},{"key":"5890_CR10","doi-asserted-by":"crossref","unstructured":"Ghorasaini M, Tsezou KI, Verhoeven A, Mohammed Y, Vlachoyiannopoulos P, Mikros E, et al. Congruence and complementarity of differential mobility spectrometry and NMR spectroscopy for plasma lipidomics. 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