{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T18:41:33Z","timestamp":1755801693855,"version":"3.44.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"21","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202536"],"award-info":[{"award-number":["82202536"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Entrusted Project by Enterprises and Tianjin Medical University General Hospital","award":["2023032"],"award-info":[{"award-number":["2023032"]}]},{"name":"Tianjin Health and Hygiene General Project","award":["25YFKFYS00940"],"award-info":[{"award-number":["25YFKFYS00940"]}]},{"name":"Tianjin Key R&D Program, Special Project for Research and Development of Science and Technology Service Industry","award":["25YFKFYS00940"],"award-info":[{"award-number":["25YFKFYS00940"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1007\/s00216-025-05998-7","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T12:16:06Z","timestamp":1751544966000},"page":"4817-4827","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and clinical application of a novel urinary cortisol extraction agent"],"prefix":"10.1007","volume":"417","author":[{"given":"Chengxue","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenting","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zichao","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizhen","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamin","family":"Chai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinzhen","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunhui","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MingFeng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5051-6476","authenticated-orcid":false,"given":"Wei","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"issue":"1898","key":"5998_CR1","doi-asserted-by":"publisher","first-page":"20220501","DOI":"10.1098\/rstb.2022.0501","volume":"379","author":"B Jimeno","year":"2024","unstructured":"Jimeno B, Rubalcaba JG. Modelling the role of glucocorticoid receptor as mediator of endocrine responses to environmental challenge. Philos Trans R Soc Lond B Biol Sci. 2024;379(1898):20220501. https:\/\/doi.org\/10.1098\/rstb.2022.0501.","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"issue":"3","key":"5998_CR2","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1111\/imm.13669","volume":"170","author":"J Luo","year":"2023","unstructured":"Luo J, Zhou C, Wang S, Tao S, Liao Y, Shi Z, Tang Z, Wu Y, Liu Y, Yang P. Cortisol synergizing with endoplasmic reticulum stress induces regulatory T-cell dysfunction. Immunology. 2023;170(3):334\u201343. https:\/\/doi.org\/10.1111\/imm.13669.","journal-title":"Immunology"},{"issue":"3","key":"5998_CR3","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1177\/0004563216687335","volume":"54","author":"N El-Farhan","year":"2017","unstructured":"El-Farhan N, Rees DA, Evans C. Measuring cortisol in serum, urine and saliva - are our assays good enough? Ann Clin Biochem. 2017;54(3):308\u201322. https:\/\/doi.org\/10.1177\/0004563216687335.","journal-title":"Ann Clin Biochem"},{"issue":"4","key":"5998_CR4","doi-asserted-by":"publisher","first-page":"599","DOI":"10.3803\/EnM.2022.1549","volume":"37","author":"MH Choi","year":"2022","unstructured":"Choi MH. Clinical and technical aspects in free cortisol measurement. Endocrinol Metab (Seoul). 2022;37(4):599\u2013607. https:\/\/doi.org\/10.3803\/EnM.2022.1549.","journal-title":"Endocrinol Metab (Seoul)"},{"issue":"1","key":"5998_CR5","doi-asserted-by":"publisher","first-page":"29","DOI":"10.3343\/alm.2024.44.1.29","volume":"44","author":"D Mu","year":"2024","unstructured":"Mu D, Fang J, Yu S, Ma Y, Cheng J, Hu Y, Song A, Zhao F, Zhang Q, Qi Z, Zhang K, Xia L, Qiu L, Zhu H, Cheng X. Comparison of direct and extraction immunoassay methods with liquid chromatography-tandem mass spectrometry measurement of urinary free cortisol for the diagnosis of Cushing\u2019s syndrome. Ann Lab Med. 2024;44(1):29\u201337. https:\/\/doi.org\/10.3343\/alm.2024.44.1.29.","journal-title":"Ann Lab Med"},{"issue":"2","key":"5998_CR6","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/j.cmet.2006.07.002","volume":"4","author":"H Oster","year":"2006","unstructured":"Oster H, Damerow S, Kiessling S, Jakubcakova V, Abraham D, Tian J, Hoffmann MW, Eichele G. The circadian rhythm of glucocorticoids is regulated by a gating mechanism residing in the adrenal cortical clock. Cell Metab. 2006;4(2):163\u201373. https:\/\/doi.org\/10.1016\/j.cmet.2006.07.002.","journal-title":"Cell Metab"},{"issue":"3","key":"5998_CR7","doi-asserted-by":"publisher","first-page":"bnaa002","DOI":"10.1210\/endrev\/bnaa002","volume":"41","author":"SL Lightman","year":"2020","unstructured":"Lightman SL, Birnie MT, Conway-Campbell BL. Dynamics of ACTH and cortisol secretion and implications for disease. Endocr Rev. 2020;41(3):bnaa002. https:\/\/doi.org\/10.1210\/endrev\/bnaa002.","journal-title":"Endocr Rev"},{"key":"5998_CR8","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.jsbmb.2019.04.004","volume":"190","author":"A O\u00dfwald","year":"2019","unstructured":"O\u00dfwald A, Wang R, Beuschlein F, Hartmann MF, Wudy SA, Bidlingmaier M, Zopp S, Reincke M, Ritzel K. Performance of LC-MS\/MS and immunoassay based 24-h urine free cortisol in the diagnosis of Cushing\u2019s syndrome. J Steroid Biochem Mol Biol. 2019;190:193\u20137. https:\/\/doi.org\/10.1016\/j.jsbmb.2019.04.004.","journal-title":"J Steroid Biochem Mol Biol"},{"issue":"1","key":"5998_CR9","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1111\/cen.14200","volume":"93","author":"P Perrin","year":"2020","unstructured":"Perrin P, Plotton I, Berthiller J, Borson-Chazot F, Raverot G, Raverot V. Urinary free cortisol: an automated immunoassay without extraction for diagnosis of Cushing\u2019s syndrome and follow-up of patients treated by anticortisolic drugs. Clin Endocrinol (Oxf). 2020;93(1):76\u20138. https:\/\/doi.org\/10.1111\/cen.14200.","journal-title":"Clin Endocrinol (Oxf)"},{"issue":"07","key":"5998_CR10","first-page":"717","volume":"47","author":"W Guo","year":"2024","unstructured":"Guo W, Li Y, Jiang N, Wu JXW. Is clinical mass spectrometry the future of hormone testing?(Chinese). Chin J Lab Med. 2024;47(07):717\u201321.","journal-title":"Chin J Lab Med"},{"issue":"3","key":"5998_CR11","first-page":"285","volume":"26","author":"H Huang","year":"2005","unstructured":"Huang H, Xie F, Zhang F, Xiong YMT. Determination of urinary free cortisol by RIA and establishment of normal reference range. Sichuan Med. 2005;26(3):285\u20136.","journal-title":"Sichuan Med"},{"issue":"6","key":"5998_CR12","first-page":"524","volume":"32","author":"Q Zhu","year":"2017","unstructured":"Zhu Q, Zhu F, Zhao J, Xu G, Shao J, Cao RHL. Establishment and performance evaluation of liquid-chromatography tandem-mass spectrometry for determining serum cortisone. Lab Med. 2017;32(6):524\u201330.","journal-title":"Lab Med"},{"key":"5998_CR13","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1007\/978-1-0716-2565-1_11","volume":"2546","author":"JA Ray","year":"2022","unstructured":"Ray JA, Kish-Trier E, Johnson LM. Measurement of urinary free cortisol and cortisone by LC-MS\/MS. Methods Mol Biol. 2022;2546:119\u201328. https:\/\/doi.org\/10.1007\/978-1-0716-2565-1_11.","journal-title":"Methods Mol Biol"},{"key":"5998_CR14","doi-asserted-by":"publisher","first-page":"462361","DOI":"10.1016\/j.chroma.2021.462361","volume":"1652","author":"J Grau","year":"2021","unstructured":"Grau J, Benede JL, Chisvert A, Salvador A. Modified magnetic-based solvent-assisted dispersive solid-phase extraction: application to the determination of cortisol and cortisone in human saliva. J Chromatogr A. 2021;1652:462361. https:\/\/doi.org\/10.1016\/j.chroma.2021.462361.","journal-title":"J Chromatogr A"},{"key":"5998_CR15","doi-asserted-by":"publisher","unstructured":"Bonnet-Serrano F, Nakib S, Zientek C, Guignat L, Guibourdenche J, Bertherat J, Menet MC. Urinary free cortisol determination and interferences studies using liquid chromatography coupled to tandem mass spectrometry after on-line solid phase extraction based on Turboflow(TM) chromatography. Metabolites. 2023;13(10). https:\/\/doi.org\/10.3390\/metabo13101063.","DOI":"10.3390\/metabo13101063"},{"issue":"11","key":"5998_CR16","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1007\/s40618-019-01050-5","volume":"42","author":"L Bianchi","year":"2019","unstructured":"Bianchi L, Campi B, Sessa MR, De Marco G, Ferrarini E, Zucchi R, Marcocci C, Vitti P, Manetti L, Saba A, Agretti P. Measurement of urinary free cortisol by LC-MS-MS: adoption of a literature reference range and comparison with our current immunometric method. J Endocrinol Invest. 2019;42(11):1299\u2013305. https:\/\/doi.org\/10.1007\/s40618-019-01050-5.","journal-title":"J Endocrinol Invest"},{"issue":"6","key":"5998_CR17","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1016\/j.beem.2013.10.008","volume":"27","author":"U Turpeinen","year":"2013","unstructured":"Turpeinen U, H\u00e4m\u00e4l\u00e4inen E. Determination of cortisol in serum, saliva and urine. Best Pract Res Clin Endocrinol Metab. 2013;27(6):795\u2013801. https:\/\/doi.org\/10.1016\/j.beem.2013.10.008.","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"issue":"4","key":"5998_CR18","first-page":"436","volume":"28","author":"Y Wu","year":"2022","unstructured":"Wu Y, Huang W, Jia C, Hu DWL. Improvement of extraction method and analysis of the interferences in the detection of urinary free cortisol. J Tianjin Med Univ. 2022;28(4):436\u201341.","journal-title":"J Tianjin Med Univ"},{"key":"5998_CR19","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.jes.2023.05.029","volume":"139","author":"M Wu","year":"2024","unstructured":"Wu M, Zhao D, Gu B, Wang Z, Hu J, Yu Z, Yu J. Efficient degradation of aqueous dichloromethane by an enhanced microbial electrolysis cell: degradation kinetics, microbial community and metabolic mechanisms. J Environ Sci (China). 2024;139:150\u20139. https:\/\/doi.org\/10.1016\/j.jes.2023.05.029.","journal-title":"J Environ Sci (China)"},{"issue":"2","key":"5998_CR20","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1021\/acscentsci.0c00100","volume":"6","author":"S Vidal","year":"2020","unstructured":"Vidal S. Safety first: a recent case of a dichloromethane injection injury. ACS Cent Sci. 2020;6(2):83\u20136. https:\/\/doi.org\/10.1021\/acscentsci.0c00100.","journal-title":"ACS Cent Sci"},{"issue":"3","key":"5998_CR21","first-page":"35","volume":"32","author":"J Li","year":"2021","unstructured":"Li J, Zheng X, Zhao R, Liu MJZ. Validation of the performance of ethyl acetate extraction for free cortisol in urine. Med Laboratory Sci and Clin. 2021;32(3):35\u201340.","journal-title":"Med Laboratory Sci and Clin"},{"issue":"8","key":"5998_CR22","doi-asserted-by":"publisher","first-page":"3589","DOI":"10.1111\/1750-3841.15835","volume":"86","author":"G Zhu","year":"2021","unstructured":"Zhu G, Xiao Z, Zhu G. Fabrication and characterization of ethyl acetate-hydroxypropyl-beta-cyclodextrin inclusion complex. J Food Sci. 2021;86(8):3589\u201397. https:\/\/doi.org\/10.1111\/1750-3841.15835.","journal-title":"J Food Sci"},{"key":"5998_CR23","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.jpba.2017.09.012","volume":"146","author":"Z Shang","year":"2017","unstructured":"Shang Z, Cai W, Cao Y, Wang F, Wang Z, Lu J, Zhang J. An integrated strategy for rapid discovery and identification of the sequential piperine metabolites in rats using ultra high-performance liquid chromatography\/high resolution mass spectrometery. J Pharm Biomed Anal. 2017;146:387\u2013401. https:\/\/doi.org\/10.1016\/j.jpba.2017.09.012.","journal-title":"J Pharm Biomed Anal"},{"key":"5998_CR24","doi-asserted-by":"publisher","first-page":"135331","DOI":"10.1016\/j.foodchem.2022.135331","volume":"410","author":"F Rezaei","year":"2023","unstructured":"Rezaei F, Eikani MH, Nosratinia F, Bidaroni HH. Optimization of ethanol-modified subcritical water extraction of curcuminoids from turmeric (Curcuma longa L.) rhizomes: comparison with conventional techniques. Food Chem. 2023;410:135331. https:\/\/doi.org\/10.1016\/j.foodchem.2022.135331.","journal-title":"Food Chem"},{"issue":"1","key":"5998_CR25","doi-asserted-by":"publisher","first-page":"8551","DOI":"10.1038\/s41598-025-91441-x","volume":"15","author":"A Allay","year":"2025","unstructured":"Allay A, Benkirane C, Ben Moumen A, Fauconnier ML, Bouakline H, Nkengurutse J, Serghini Caid H, Elamrani A, Mansouri F. Optimizing ethanol-modified supercritical CO(2) extraction for enhanced bioactive compound recovery in hemp seed oil. Sci Rep. 2025;15(1):8551. https:\/\/doi.org\/10.1038\/s41598-025-91441-x.","journal-title":"Sci Rep"},{"key":"5998_CR26","doi-asserted-by":"publisher","first-page":"113655","DOI":"10.1016\/j.foodres.2023.113655","volume":"176","author":"Y Niu","year":"2024","unstructured":"Niu Y, Yang Y, Mao C, Xiao Z. Effects of gallic acid on the release of aroma compounds in Moutai Baijiu. Food Res Int. 2024;176:113655. https:\/\/doi.org\/10.1016\/j.foodres.2023.113655.","journal-title":"Food Res Int"},{"issue":"3","key":"5998_CR27","first-page":"306","volume":"21","author":"Y Lin","year":"2014","unstructured":"Lin Y, Huang J, Hu Y, Huang Y, Gao Q. Methodology verification of electrochemical luminescence immunoassay for determination 0f 24 hours urinary free cortisol (Chinese). Labeled Immunoassays & Clin Med. 2014;21(3):306\u20139.","journal-title":"Labeled Immunoassays & Clin Med"},{"key":"5998_CR28","doi-asserted-by":"publisher","unstructured":"Casals G, Ballesteros MA, Zamora A, Martinez I, Fernandez-Varo G, Mora M, Hanzu FA, Morales-Ruiz M (2024) LC-HRMS and GC-MS profiling of urine free cortisol, cortisone, 6beta-, and 18-hydroxycortisol for the evaluation of glucocorticoid and mineralocorticoid disorders. Biomolecules 14(5). https:\/\/doi.org\/10.3390\/biom14050558","DOI":"10.3390\/biom14050558"},{"key":"5998_CR29","unstructured":"Luo W, Dong Z, Jia C, Huang X, Hao Q, Xie H, Chai M, Wang X, Cao F, Dong L. A urinary cortisol extraction composition, extraction method and extraction device (Chinese). 2024. CN202410430467[P][2024\u201307\u201325]."},{"issue":"2","key":"5998_CR30","first-page":"95","volume":"19","author":"GL Horowitz","year":"2008","unstructured":"Horowitz GL. Reference intervals: Practical Aspects. EJIFCC. 2008;19(2):95\u2013105.","journal-title":"EJIFCC."},{"issue":"12","key":"5998_CR31","first-page":"1128","volume":"34","author":"M Gu","year":"2019","unstructured":"Gu M, Sun Y, Jia C, Fan M, Rrn N, Zhang H, Yuan HWL. Comparability of routine biochemical indexes between dry chemistry and wet chemistry analysis systems (Chinese). Laboratory Medicine. 2019;34(12):1128\u201332.","journal-title":"Laboratory Medicine"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-05998-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-025-05998-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-05998-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T13:02:17Z","timestamp":1755781337000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-025-05998-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":31,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["5998"],"URL":"https:\/\/doi.org\/10.1007\/s00216-025-05998-7","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"type":"print","value":"1618-2642"},{"type":"electronic","value":"1618-2650"}],"subject":[],"published":{"date-parts":[[2025,7,3]]},"assertion":[{"value":"11 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 June 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 June 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 July 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Informed consent was obtained from all individuals included in this study, or their legal guardians or wards. This study was approved by the Medical Ethics Committee of Tianjin Medical University General Hospital: IRB2023-KY-244.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research ethics"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}