{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:43:54Z","timestamp":1790811834194,"version":"4.1.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"26","license":[{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1007\/s00216-025-06083-9","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T15:53:26Z","timestamp":1756310006000},"page":"5885-5893","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A ratiometric fluorescent aptamer assay based on RCA amplification mult-G-quadruplex"],"prefix":"10.1007","volume":"417","author":[{"given":"Xinyu","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiling","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguang","family":"Suo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huali","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,8,27]]},"reference":[{"issue":"48","key":"6083_CR1","doi-asserted-by":"publisher","first-page":"26865","DOI":"10.1021\/acsami.5b09300","volume":"7","author":"C Wang","year":"2015","unstructured":"Wang C, Qian J, Wang K, Hua M, Liu Q, Hao N, You T, Huang X. Nitrogen-doped graphene quantum dots@SiO2 nanoparticles as electrochemiluminescence and fluorescence signal indicators for magnetically controlled aptasensor with dual detection channels. Acs Appl Mater Interfaces. 2015;7(48):26865\u201373. https:\/\/doi.org\/10.1021\/acsami.5b09300.","journal-title":"Acs Appl Mater Interfaces"},{"key":"6083_CR2","doi-asserted-by":"publisher","first-page":"131415","DOI":"10.1016\/j.foodchem.2021.131415","volume":"373","author":"Q Huang","year":"2022","unstructured":"Huang Q, Lin X, Chen D, Tong Q. Carbon dots\/\u03b1-Fe2O3-Fe3O4 nanocomposite: efficient synthesis and application as a novel electrochemical aptasensor for the ultrasensitive determination of aflatoxin b1. Food Chem. 2022;373:131415. https:\/\/doi.org\/10.1016\/j.foodchem.2021.131415.","journal-title":"Food Chem"},{"key":"6083_CR3","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.fct.2018.11.047","volume":"124","author":"BR Rushing","year":"2019","unstructured":"Rushing BR, Selim MI. Aflatoxin B1: A review on metabolism, toxicity, occurrence in food, occupational exposure, and detoxification methods. Food Chem Toxicol. 2019;124:81\u2013100. https:\/\/doi.org\/10.1016\/j.fct.2018.11.047.","journal-title":"Food Chem Toxicol"},{"key":"6083_CR4","doi-asserted-by":"publisher","first-page":"125569","DOI":"10.1016\/j.talanta.2023.125569","volume":"270","author":"H Li","year":"2024","unstructured":"Li H, Shang Q, Zhang L, Mao J, Zhang Q, Li P. Europium nanospheres based ultrasensitive fluorescence immunosensor for aflatoxin B1 determination in feed. Talanta (Oxford). 2024;270:125569. https:\/\/doi.org\/10.1016\/j.talanta.2023.125569.","journal-title":"Talanta (Oxford)"},{"issue":"3","key":"6083_CR5","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3390\/toxins12030173","volume":"12","author":"A Chrouda","year":"2020","unstructured":"Chrouda A, Zinoubi K, Soltane R, Alzahrani N, Osman G, Al-Ghamdi YO, Qari S, Al Mahri A, Algethami FK, Majdoub H, Jaffrezic RN. An Acetylcholinesterase Inhibition-Based Biosensor for Aflatoxin B1 Detection Using Sodium Alginate as an Immobilization Matrix. Toxins (Basel). 2020;12(3):173. https:\/\/doi.org\/10.3390\/toxins12030173.","journal-title":"Toxins (Basel)"},{"key":"6083_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.aca.2019.04.032","volume":"1069","author":"Z Xue","year":"2019","unstructured":"Xue Z, Zhang Y, Yu W, Zhang J, Wang J, Wan F, Kim Y, Liu Y, Kou X. Recent advances in aflatoxin B1 detection based on nanotechnology and nanomaterials-A review. Anal Chim Acta. 2019;1069:1\u201327. https:\/\/doi.org\/10.1016\/j.aca.2019.04.032.","journal-title":"Anal Chim Acta"},{"key":"6083_CR7","doi-asserted-by":"publisher","first-page":"104685","DOI":"10.1016\/j.fbio.2024.104685","volume":"61","author":"J Xu","year":"2024","unstructured":"Xu J, Yang W, Liu Y. A novel biomass carbon dots ratiometric fluorescence probe coupled with molecularly imprinted polymer for selective detection of aflatoxin b1. Food Biosci. 2024;61:104685. https:\/\/doi.org\/10.1016\/j.fbio.2024.104685.","journal-title":"Food Biosci"},{"key":"6083_CR8","doi-asserted-by":"publisher","first-page":"109697","DOI":"10.1016\/j.ecoenv.2019.109697","volume":"186","author":"H Zhou","year":"2019","unstructured":"Zhou H, Wang J, Ma L, Chen L, Guo T, Zhang Y, Dai H, Yu Y. Oxidative dna damage and multi-organ pathologies in male mice subchronically treated with aflatoxin B1. Ecotoxicol Environ Saf. 2019;186:109697. https:\/\/doi.org\/10.1016\/j.ecoenv.2019.109697.","journal-title":"Ecotoxicol Environ Saf"},{"issue":"21","key":"6083_CR9","doi-asserted-by":"publisher","first-page":"11803","DOI":"10.1021\/acs.analchem.7b03451","volume":"89","author":"Y Lin","year":"2017","unstructured":"Lin Y, Zhou Q, Tang D. Dopamine-loaded liposomes for in-situ amplified photoelectrochemical immunoassay of AFB1 to enhance photocurrent of Mn2+ -doped Zn3 (OH)2V2O7 nanobelts. Anal Chem. 2017;89(21):11803\u201310. https:\/\/doi.org\/10.1021\/acs.analchem.7b03451.","journal-title":"Anal Chem"},{"key":"6083_CR10","doi-asserted-by":"publisher","first-page":"118250","DOI":"10.1016\/j.trac.2025.118250","volume":"189","author":"M Kang","year":"2025","unstructured":"Kang M, Zhao Q. Recent advances of aptamer based sensors for aflatoxins. TrAC, Trends Anal Chem. 2025;189:118250. https:\/\/doi.org\/10.1016\/j.trac.2025.118250.","journal-title":"TrAC, Trends Anal Chem"},{"key":"6083_CR11","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1038\/346818a0","volume":"346","author":"AD Ellington","year":"1990","unstructured":"Ellington AD, Szostak JW. In vitro selection of rna molecules that bind specific ligands. Nature. 1990;346:818\u201322.","journal-title":"Nature"},{"key":"6083_CR12","doi-asserted-by":"crossref","unstructured":"Tuerk C, Gold L. Systematic evolution of ligands by exponential enrichment: rna ligands to bacteriophage t4 dna polymerase. Science. 1990;249(4968):505\u2013510.","DOI":"10.1126\/science.2200121"},{"key":"6083_CR13","doi-asserted-by":"publisher","first-page":"125642","DOI":"10.1016\/j.talanta.2024.125642","volume":"271","author":"H Yu","year":"2024","unstructured":"Yu H, Zhao Q. Sensitive electrochemical sensor for Cd2+ with engineered short high-affinity aptamer undergoing large conformation change. Talanta. 2024;271:125642.","journal-title":"Talanta"},{"key":"6083_CR14","doi-asserted-by":"publisher","first-page":"136205","DOI":"10.1016\/j.foodchem.2023.136205","volume":"421","author":"L Lu","year":"2023","unstructured":"Lu L, Yu R, Zhang L. AFB1 colorimetric aptamer sensor for the detection of AFB1 in ten different kinds of miscellaneous beans based on gold nanoparticles and smartphone imaging. Food Chem. 2023;421:136205. https:\/\/doi.org\/10.1016\/j.foodchem.2023.136205.","journal-title":"Food Chem"},{"issue":"12","key":"6083_CR15","doi-asserted-by":"publisher","first-page":"2981","DOI":"10.1007\/s00216-018-0981-2","volume":"410","author":"C Zhou","year":"2018","unstructured":"Zhou C, Zou H, Sun C, Ren D, Xiong W, Li Y. Fluorescent aptasensor for detection of four tetracycline veterinary drugs in milk based on catalytic hairpin assembly reaction and displacement of g-quadruplex. Anal Bioanal Chem. 2018;410(12):2981\u20139. https:\/\/doi.org\/10.1007\/s00216-018-0981-2.","journal-title":"Anal Bioanal Chem"},{"key":"6083_CR16","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.aca.2019.06.035","volume":"1079","author":"A Bigdeli","year":"2019","unstructured":"Bigdeli A, Ghasemi F, Abbasi-Moayed S, Shahrajabian M, Fahimi-Kashani N, Jafarinejad S, FarahmandNejad MA, Hormozi-Nezhad MR. Ratiometric fluorescent nanoprobes for visual detection: design principles and recent advances - a review. Anal Chim Acta. 2019;1079:30\u201358.","journal-title":"Anal Chim Acta"},{"issue":"3","key":"6083_CR17","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1007\/s41664-024-00295-1","volume":"8","author":"X He","year":"2024","unstructured":"He X, Dong Y, He P, Liu C, Ren W. A biomimetic membrane-coated nanoprobe for the ratiometric fluorescence detection of phospholipase a2. J Anal Test. 2024;8(3):270\u20137. https:\/\/doi.org\/10.1007\/s41664-024-00295-1.","journal-title":"J Anal Test"},{"issue":"2","key":"6083_CR18","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1007\/s41664-024-00297-z","volume":"8","author":"D Zhang","year":"2024","unstructured":"Zhang D, Zhou G, Yang H, Wang Y, Shen L, Qiu Y, Li L, Guo L. A bifunctional-blocker-aided hybridization chain reaction lighting-up self-calibrating nanocluster fluorescence for reliable nucleic acid detection. J Anal Test. 2024;8(2):160\u20139. https:\/\/doi.org\/10.1007\/s41664-024-00297-z.","journal-title":"J Anal Test"},{"key":"6083_CR19","doi-asserted-by":"publisher","first-page":"112920","DOI":"10.1016\/j.microc.2025.112920","volume":"210","author":"Q Cheng","year":"2025","unstructured":"Cheng Q, Zhao Q. A simple fluorescence anisotropy assay for 17\u03b2-estradiol by using fluorescently labeled aptamer. Microchem J. 2025;210:112920. https:\/\/doi.org\/10.1016\/j.microc.2025.112920.","journal-title":"Microchem J"},{"key":"6083_CR20","doi-asserted-by":"publisher","first-page":"109348","DOI":"10.1016\/j.microc.2023.109348","volume":"194","author":"H Yu","year":"2023","unstructured":"Yu H, Zhao Q. Competitive fluorescence assay for Cd2+ based on aptamer structure-switching. Microchem J. 2023;194:109348. https:\/\/doi.org\/10.1016\/j.microc.2023.109348.","journal-title":"Microchem J"},{"key":"6083_CR21","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/j.bios.2014.09.092","volume":"64","author":"JR Zhang","year":"2015","unstructured":"Zhang JR, Huang WT, Zeng AL, Luo HQ, Li NB. Ethynyl and \u03c0-stacked thymine\u2013Hg2+\u2013thymine base pairs enhanced fluorescence quenching via photoinduced electron transfer and simple and sensitive mercury ion sensing. Biosens Bioelectron. 2015;64:597\u2013604. https:\/\/doi.org\/10.1016\/j.bios.2014.09.092.","journal-title":"Biosens Bioelectron"},{"key":"6083_CR22","doi-asserted-by":"publisher","first-page":"137844","DOI":"10.1016\/j.molstruc.2024.137844","volume":"1306","author":"AK Dwivedi","year":"2024","unstructured":"Dwivedi AK, Jha NS. Probing the binding and interaction of curcumin-tryptophan conjugate to g-quadruplex motif via spectroscopic and molecular docking approach. J Mol Struct. 2024;1306:137844. https:\/\/doi.org\/10.1016\/j.molstruc.2024.137844.","journal-title":"J Mol Struct"},{"key":"6083_CR23","doi-asserted-by":"publisher","first-page":"114697","DOI":"10.1016\/j.ab.2022.114697","volume":"651","author":"M Agahi","year":"2022","unstructured":"Agahi M, Rahaie M. A novel dna tweezers-based nanobiosensor for multiple detections of circulating exosomal micrornas in breast cancer. Anal Biochem. 2022;651:114697. https:\/\/doi.org\/10.1016\/j.ab.2022.114697.","journal-title":"Anal Biochem"},{"issue":"3","key":"6083_CR24","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1007\/s12539-023-00577-9","volume":"15","author":"H Zhong","year":"2023","unstructured":"Zhong H, Dong M, Gao F. G4Bank: A database of experimentally identified DNA G-quadruplex sequences. Interdiscip Sci: Comput Life Sci. 2023;15(3):515\u201323. https:\/\/doi.org\/10.1007\/s12539-023-00577-9.","journal-title":"Interdiscip Sci: Comput Life Sci"},{"issue":"20","key":"6083_CR25","doi-asserted-by":"publisher","first-page":"10835","DOI":"10.1080\/07391102.2023.2259478","volume":"42","author":"S Kaur","year":"2024","unstructured":"Kaur S, Kundu N, Sharma T, Shankaraswamy J, Saxena S. Identification of G4 motifs of various stem cell markers and their biophysical and biochemical characterization. J Biomol Struct Dyn. 2024;42(20):10835\u201344. https:\/\/doi.org\/10.1080\/07391102.2023.2259478.","journal-title":"J Biomol Struct Dyn"},{"issue":"3","key":"6083_CR26","doi-asserted-by":"publisher","first-page":"2375","DOI":"10.3390\/ijms24032375","volume":"24","author":"Y Teng","year":"2023","unstructured":"Teng Y, Zhu M, Qiu Z. G-quadruplexes in repeat expansion disorders. Int J Mol Sci. 2023;24(3):2375. https:\/\/doi.org\/10.3390\/ijms24032375.","journal-title":"Int J Mol Sci"},{"key":"6083_CR27","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.biochi.2015.09.031","volume":"118","author":"O Mendoza","year":"2015","unstructured":"Mendoza O, Elezgaray J, Mergny J. Kinetics of quadruplex to duplex conversion. Biochimie. 2015;118:225\u201333. https:\/\/doi.org\/10.1016\/j.biochi.2015.09.031.","journal-title":"Biochimie"},{"issue":"12","key":"6083_CR28","doi-asserted-by":"publisher","first-page":"5460","DOI":"10.1038\/s41596-021-00621-2","volume":"16","author":"C Yao","year":"2021","unstructured":"Yao C, Zhang R, Tang J, Yang D. Rolling circle amplification (RCA)-based DNA hydrogel. Nat Protoc. 2021;16(12):5460\u201383. https:\/\/doi.org\/10.1038\/s41596-021-00621-2.","journal-title":"Nat Protoc"},{"issue":"24","key":"6083_CR29","doi-asserted-by":"publisher","first-page":"8002","DOI":"10.1039\/D0AN01326A","volume":"145","author":"H Lee","year":"2020","unstructured":"Lee H, Kim D, Kim D. Label-free fluorometric detection of influenza viral RNA by strand displacement coupled with rolling circle amplification. Analyst. 2020;145(24):8002\u20137. https:\/\/doi.org\/10.1039\/D0AN01326A.","journal-title":"Analyst"},{"key":"6083_CR30","doi-asserted-by":"publisher","first-page":"6453","DOI":"10.1039\/c9cc01850f","volume":"55","author":"Z Yang","year":"2019","unstructured":"Yang Z, Wen Z, Peng X, Chai Y, Liang W, Yuan R. A Novel Fluorescent Assay for the Ultrasensitive Detection of Mirna-21 with the Use of G-quadruplex Structures As an Immobilization Material for a Signal Indicator. Chem Commun (Camb). 2019;55:6453\u20136. https:\/\/doi.org\/10.1039\/c9cc01850f.","journal-title":"Chem Commun (Camb)"},{"issue":"32","key":"6083_CR31","doi-asserted-by":"publisher","first-page":"11425","DOI":"10.1021\/acs.analchem.2c02578","volume":"94","author":"Z Wang","year":"2022","unstructured":"Wang Z, Li D, Tian X, Li Y, Zhang C. Single-Molecule Counting of FTO in Human Breast Tissues Based on a Rolling Circle Transcription Amplification-Driven Clustered Regularly Interspaced Short Palindromic Repeat\u2500Cas12a. Anal Chem. 2022;94(32):11425\u201332. https:\/\/doi.org\/10.1021\/acs.analchem.2c02578.","journal-title":"Anal Chem"},{"key":"6083_CR32","doi-asserted-by":"publisher","first-page":"109246","DOI":"10.1016\/j.microc.2023.109246","volume":"193","author":"Y Qi","year":"2023","unstructured":"Qi Y, Du Y, Kang Q, Yang X, Xiang H. A novel self-feedback biosensor for label-free detection of APE1 by primer exchange reaction and rolling circle amplification\/dimeric G-quadruplex. Microchem J. 2023;193:109246. https:\/\/doi.org\/10.1016\/j.microc.2023.109246.","journal-title":"Microchem J"},{"issue":"8","key":"6083_CR33","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1007\/s00604-023-05897-7","volume":"190","author":"M Qiao","year":"2023","unstructured":"Qiao M, Liu M, Wan Z, Suo Z, Liu Y, Wei M. A novel fluorescent aptasensor based on 3D porous nitrogen-sulfur co-doped carbon mesh and hybridization chain reaction for sensitive detection of ochratoxin A. Mikrochim Acta. 2023;190(8):313. https:\/\/doi.org\/10.1007\/s00604-023-05897-7.","journal-title":"Mikrochim Acta"},{"key":"6083_CR34","doi-asserted-by":"publisher","first-page":"109397","DOI":"10.1016\/j.foodcont.2022.109397","volume":"144","author":"J Xiong","year":"2023","unstructured":"Xiong J, He S, Zhang S, Qin L, Yang L, Wang Z, Zhang L, Shan W, Jiang H. A label-free aptasensor for dual-mode detection of aflatoxin b1 based on inner filter effect using silver nanoparticles and arginine-modified gold nanoclusters. Food Control. 2023;144:109397. https:\/\/doi.org\/10.1016\/j.foodcont.2022.109397.","journal-title":"Food Control"},{"key":"6083_CR35","doi-asserted-by":"publisher","first-page":"129614","DOI":"10.1016\/j.foodchem.2021.129614","volume":"356","author":"B Jia","year":"2021","unstructured":"Jia B, Liao X, Sun C, Fang L, Zhou L, Kong W. Development of a quantum dot nanobead-based fluorescent strip immunosensor for on-site detection of aflatoxin B1 in lotus seeds. Food Chem. 2021;356:129614. https:\/\/doi.org\/10.1016\/j.foodchem.2021.129614.","journal-title":"Food Chem"},{"issue":"3","key":"6083_CR36","doi-asserted-by":"publisher","first-page":"87","DOI":"10.3390\/chemosensors8030087","volume":"8","author":"FL Migliorini","year":"2020","unstructured":"Migliorini FL, Santos DMD, Soares AC, Mattoso LHC, Oliveira ON, Correa DS. Design of A Low-Cost and Disposable Paper-Based Immunosensor for the Rapid and Sensitive Detection of Aflatoxin B1. Chemosensors (Basel). 2020;8(3):87. https:\/\/doi.org\/10.3390\/chemosensors8030087.","journal-title":"Chemosensors (Basel)"},{"key":"6083_CR37","doi-asserted-by":"publisher","first-page":"124000","DOI":"10.1016\/j.talanta.2022.124000","volume":"253","author":"K Kunene","year":"2023","unstructured":"Kunene K, Sayegh S, Weber M, Sabela M, Voiry D, Iatsunskyi I, Coy E, Kanchi S, Bisetty K, Bechelany M. Smart electrochemical immunosensing of aflatoxin B1 based on a palladium nanoparticle-boron nitride-coated carbon felt electrode for the wine industry. Talanta (Oxford). 2023;253:124000. https:\/\/doi.org\/10.1016\/j.talanta.2022.124000.","journal-title":"Talanta (Oxford)"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-06083-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-025-06083-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-06083-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T02:05:42Z","timestamp":1760666742000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-025-06083-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,27]]},"references-count":37,"journal-issue":{"issue":"26","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["6083"],"URL":"https:\/\/doi.org\/10.1007\/s00216-025-06083-9","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,27]]},"assertion":[{"value":"30 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}