{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:03:28Z","timestamp":1784127808495,"version":"3.55.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"29","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515220171"],"award-info":[{"award-number":["2023A1515220171"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515220189"],"award-info":[{"award-number":["2023A1515220189"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s00216-025-06152-z","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T04:34:42Z","timestamp":1761798882000},"page":"6643-6653","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Highly sensitive PfAgo-based biosensor\u00a0for the detection of mutations in the 23S rRNA gene of macrolide-resistant Mycoplasma pneumoniae"],"prefix":"10.1007","volume":"417","author":[{"given":"Houqi","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyun","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhui","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoliang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zishi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luxin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-046X","authenticated-orcid":false,"given":"Zhangquan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,30]]},"reference":[{"key":"6152_CR1","doi-asserted-by":"publisher","unstructured":"Miyashita N. Contemporary concise review 2024: respiratory infections. Respirology. 2025. https:\/\/doi.org\/10.1111\/resp.70113.","DOI":"10.1111\/resp.70113"},{"issue":"7","key":"6152_CR2","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.3201\/eid2607.200017","volume":"26","author":"Y Chen","year":"2020","unstructured":"Chen Y, Hsu W, Chang T. Macrolide-resistant Mycoplasma pneumoniae infections in pediatric community-acquired pneumonia. Emerg Infect Dis. 2020;26(7):1382\u201391. https:\/\/doi.org\/10.3201\/eid2607.200017.","journal-title":"Emerg Infect Dis"},{"key":"6152_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1496521","volume":"15","author":"P Xie","year":"2025","unstructured":"Xie P, Zhang Y, Qin Y, Fang Y, Yang N, Bai Y, et al. Macrolide resistance in Mycoplasma pneumoniae in adult patients. Front Cell Infect Microbiol. 2025;15:1496521. https:\/\/doi.org\/10.3389\/fcimb.2025.1496521.","journal-title":"Front Cell Infect Microbiol"},{"issue":"7\u20138","key":"6152_CR4","doi-asserted-by":"publisher","first-page":"487","DOI":"10.46747\/cfp.710708487","volume":"71","author":"C Smith","year":"2025","unstructured":"Smith C, Ng K, Sih K, McAlpine A, Goldman RD. Resistant Mycoplasma pneumoniae in children. Can Fam Physician. 2025;71(7\u20138):487\u20139. https:\/\/doi.org\/10.46747\/cfp.710708487.","journal-title":"Can Fam Physician"},{"issue":"4","key":"6152_CR5","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlaf124","volume":"7","author":"C Yan","year":"2025","unstructured":"Yan C, Chen Y, Ren X, Jia X, Du B, Zhao H, et al. In vitro antimicrobial susceptibility of Mycoplasma pneumoniae isolates across different regions of China in 2023. JAC-Antimicrobial Resistance. 2025;7(4):dlaf124. https:\/\/doi.org\/10.1093\/jacamr\/dlaf124.","journal-title":"JAC-Antimicrobial Resistance"},{"issue":"4","key":"6152_CR6","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.resinv.2025.04.009","volume":"63","author":"N Miyashita","year":"2025","unstructured":"Miyashita N, Ogata M, Fukuda N, Yamura A, Ito T. Macrolide-resistant Mycoplasma pneumoniae infection prevalence increases again in Osaka. Respir Investig. 2025;63(4):517\u201320. https:\/\/doi.org\/10.1016\/j.resinv.2025.04.009.","journal-title":"Respir Investig"},{"key":"6152_CR7","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/9328177","volume":"2024","author":"Q Chen","year":"2024","unstructured":"Chen Q, Hu T, Wu L, Chen L. Clinical features and biomarkers for early prediction of refractory Mycoplasma pneumoniae pneumonia in children. Emerg Med Int. 2024;2024:9328177. https:\/\/doi.org\/10.1155\/2024\/9328177.","journal-title":"Emerg Med Int"},{"issue":"1","key":"6152_CR8","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-55311-2","volume":"14","author":"P Li","year":"2024","unstructured":"Li P, Wang W, Zhang X, Pan J, Gong L. Observational retrospective clinical study on clinical features of macrolide-resistant Mycoplasma pneumoniae pneumonia in Chinese pediatric cases. Sci Rep. 2024;14(1):5632. https:\/\/doi.org\/10.1038\/s41598-024-55311-2.","journal-title":"Sci Rep"},{"issue":"1","key":"6152_CR9","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1186\/s12879-025-10964-w","volume":"25","author":"W Gu","year":"2025","unstructured":"Gu W, Li Z, Han E, Kuai X, Hua S, Gao S, et al. Genotyping and refractory risk factors of mycoplasma pneumoniae pneumonia in Suzhou, China. BMC Infect Dis. 2025;25(1):561. https:\/\/doi.org\/10.1186\/s12879-025-10964-w.","journal-title":"BMC Infect Dis"},{"key":"6152_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12010062","author":"L Liu","year":"2023","unstructured":"Liu L, Xiang C, Zhang Y, He L, Meng F, Gong J, et al. A novel detection procedure for mutations in the 23S rRNA gene of macrolide-resistant Mycoplasma pneumoniae with two non-overlapping probes amplification assay. Microorganisms. 2023. https:\/\/doi.org\/10.3390\/microorganisms12010062.","journal-title":"Microorganisms"},{"issue":"4","key":"6152_CR11","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.molp.2025.03.002","volume":"18","author":"F Li","year":"2025","unstructured":"Li F, Tan X, Li S, Chen S, Liu L, Huang J, et al. Superdecode: an integrated toolkit for analyzing mutations induced by genome editing. Mol Plant. 2025;18(4):690\u2013702. https:\/\/doi.org\/10.1016\/j.molp.2025.03.002.","journal-title":"Mol Plant"},{"key":"6152_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2024.101268","volume":"97","author":"CT Wittwer","year":"2024","unstructured":"Wittwer CT, Hemmert AC, Kent JO, Rejali NA. DNA melting analysis. Mol Aspects Med. 2024;97:101268. https:\/\/doi.org\/10.1016\/j.mam.2024.101268.","journal-title":"Mol Aspects Med"},{"key":"6152_CR13","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18112316","author":"M S\u0142omka","year":"2017","unstructured":"S\u0142omka M, Sobalska-Kwapis M, Wachulec M, Bartosz G, Strapagiel D. High resolution melting (HRM) for high-throughput genotyping-limitations and caveats in practical case studies. Int J Mol Sci. 2017. https:\/\/doi.org\/10.3390\/ijms18112316.","journal-title":"Int J Mol Sci"},{"key":"6152_CR14","doi-asserted-by":"publisher","DOI":"10.3390\/bios11110427","author":"A Wang","year":"2021","unstructured":"Wang A, Wu Z, Huang Y, Zhou H, Wu L, Jia C, et al. A 3d-printed microfluidic device for qPCR detection of macrolide-resistant mutations of Mycoplasma pneumoniae. Biosensors. 2021. https:\/\/doi.org\/10.3390\/bios11110427.","journal-title":"Biosensors"},{"issue":"1","key":"6152_CR15","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4228-4","volume":"19","author":"D Guo","year":"2019","unstructured":"Guo D, Hu W, Xu B, Li J, Li D, Li S, et al. Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma pneumoniae. BMC Infect Dis. 2019;19(1):616. https:\/\/doi.org\/10.1186\/s12879-019-4228-4.","journal-title":"BMC Infect Dis"},{"issue":"7","key":"6152_CR16","doi-asserted-by":"publisher","first-page":"4054","DOI":"10.1093\/nar\/gkab182","volume":"49","author":"E Kropocheva","year":"2021","unstructured":"Kropocheva E, Kuzmenko A, Aravin AA, Esyunina D, Kulbachinskiy A. A programmable PAgo nuclease with universal guide and target specificity from the mesophilic bacterium Kurthia massiliensis. Nucleic Acids Res. 2021;49(7):4054\u201365. https:\/\/doi.org\/10.1093\/nar\/gkab182.","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"6152_CR17","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/j.tibtech.2022.11.002","volume":"41","author":"Y Li","year":"2023","unstructured":"Li Y, Liao D, Kou J, Tong Y, Daniels LC, Man S, et al. Comparison of CRISPR\/Cas and Argonaute for nucleic acid tests. Trends Biotechnol. 2023;41(5):595\u20139. https:\/\/doi.org\/10.1016\/j.tibtech.2022.11.002.","journal-title":"Trends Biotechnol"},{"issue":"8","key":"6152_CR18","doi-asserted-by":"publisher","first-page":"1739","DOI":"10.1007\/s12033-023-00819-7","volume":"66","author":"K Pandya","year":"2024","unstructured":"Pandya K, Jagani D, Singh N. CRISPR-Cas systems: programmable nuclease revolutionizing the molecular diagnosis. Mol Biotechnol. 2024;66(8):1739\u201353. https:\/\/doi.org\/10.1007\/s12033-023-00819-7.","journal-title":"Mol Biotechnol"},{"issue":"30","key":"6152_CR19","doi-asserted-by":"publisher","first-page":"7521","DOI":"10.1007\/s00216-021-03716-7","volume":"413","author":"K Yang","year":"2021","unstructured":"Yang K, Liang Y, Li Y, Liu Q, Zhang W, Yin D, et al. Reverse transcription-enzymatic recombinase amplification coupled with CRISPR-Cas12a for rapid detection and differentiation of PEDV wild-type strains and attenuated vaccine strains. Anal Bioanal Chem. 2021;413(30):7521\u20139. https:\/\/doi.org\/10.1007\/s00216-021-03716-7.","journal-title":"Anal Bioanal Chem"},{"issue":"39","key":"6152_CR20","doi-asserted-by":"publisher","first-page":"12700","DOI":"10.1021\/acs.jafc.2c04548","volume":"70","author":"C Yan","year":"2022","unstructured":"Yan C, Shi G, Chen J. Fluorescent detection of two pesticides based on CRISPR-Cas12a and its application for the construction of four molecular logic gates. J Agric Food Chem. 2022;70(39):12700\u20137. https:\/\/doi.org\/10.1021\/acs.jafc.2c04548.","journal-title":"J Agric Food Chem"},{"key":"6152_CR21","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2022.124210","volume":"255","author":"J Pan","year":"2023","unstructured":"Pan J, Deng F, Liu Z, Zeng L, Chen J. Construction of molecular logic gates using heavy metal ions as inputs based on catalytic hairpin assembly and CRISPR-Cas12a. Talanta. 2023;255:124210. https:\/\/doi.org\/10.1016\/j.talanta.2022.124210.","journal-title":"Talanta"},{"issue":"10","key":"6152_CR22","doi-asserted-by":"publisher","first-page":"3623","DOI":"10.1021\/acssensors.3c01232","volume":"8","author":"Z Yang","year":"2023","unstructured":"Yang Z, Mao S, Wang L, Fu S, Dong Y, Jaffrezic-Renault N, et al. CRISPR\/Cas and argonaute-based biosensors for pathogen detection. ACS Sens. 2023;8(10):3623\u201342. https:\/\/doi.org\/10.1021\/acssensors.3c01232.","journal-title":"ACS Sens"},{"issue":"9","key":"6152_CR23","doi-asserted-by":"publisher","first-page":"5016","DOI":"10.1093\/nar\/gkaa208","volume":"48","author":"T Karvelis","year":"2020","unstructured":"Karvelis T, Bigelyte G, Young JK, Hou Z, Zedaveinyte R, Budre K, et al. Pam recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage. Nucleic Acids Res. 2020;48(9):5016\u201323. https:\/\/doi.org\/10.1093\/nar\/gkaa208.","journal-title":"Nucleic Acids Res"},{"issue":"11","key":"6152_CR24","doi-asserted-by":"publisher","first-page":"5665","DOI":"10.1021\/acssensors.4c01631","volume":"9","author":"Y Li","year":"2024","unstructured":"Li Y, Zhao L, Wang J, Ma L, Bai Y, Feng F. Argonaute-based nucleic acid detection technology: advantages, current status, challenges, and perspectives. ACS Sens. 2024;9(11):5665\u201382. https:\/\/doi.org\/10.1021\/acssensors.4c01631.","journal-title":"ACS Sens"},{"issue":"3","key":"6152_CR25","doi-asserted-by":"publisher","DOI":"10.3390\/biom12030355","volume":"12","author":"X Jiang","year":"2022","unstructured":"Jiang X, Liu Y, Liu Q, Ma L. Characterization of a programmable Argonaute nuclease from the mesophilic bacterium Rummeliibacillus suwonensis. Biomolecules. 2022;12(3):355. https:\/\/doi.org\/10.3390\/biom12030355.","journal-title":"Biomolecules"},{"issue":"88","key":"6152_CR26","doi-asserted-by":"publisher","first-page":"13219","DOI":"10.1039\/c9cc07339f","volume":"55","author":"R He","year":"2019","unstructured":"He R, Wang L, Wang F, Li W, Liu Y, Li A, et al. Pyrococcus furiosus argonaute-mediated nucleic acid detection. Chem Commun (Camb). 2019;55(88):13219\u201322. https:\/\/doi.org\/10.1039\/c9cc07339f.","journal-title":"Chem Commun (Camb)"},{"issue":"29","key":"6152_CR27","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202416631","volume":"12","author":"L Wang","year":"2025","unstructured":"Wang L, Xie X, Huang F, Wei Q, Cai T, Yu N, et al. An engineered PfAgo with wide catalytic temperature range and substrate spectrum. Adv Sci. 2025;12(29):e2416631. https:\/\/doi.org\/10.1002\/advs.202416631.","journal-title":"Adv Sci"},{"issue":"13","key":"6152_CR28","doi-asserted-by":"publisher","first-page":"7529","DOI":"10.1093\/nar\/gkac565","volume":"50","author":"L Zheng","year":"2022","unstructured":"Zheng L, Lu H, Zan B, Li S, Liu H, Liu Z, et al. Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity. Nucleic Acids Res. 2022;50(13):7529\u201344. https:\/\/doi.org\/10.1093\/nar\/gkac565.","journal-title":"Nucleic Acids Res"},{"key":"6152_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112932","volume":"177","author":"F Wang","year":"2021","unstructured":"Wang F, Yang J, He R, Yu X, Chen S, Liu Y, et al. PfAgo-based detection of SARS-CoV-2. Biosens Bioelectron. 2021;177:112932. https:\/\/doi.org\/10.1016\/j.bios.2020.112932.","journal-title":"Biosens Bioelectron"},{"issue":"7","key":"6152_CR30","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-024-06516-9","volume":"191","author":"Y Xiang","year":"2024","unstructured":"Xiang Y, Ke W, Qin Y, Zhou B, Hu Y. PfAgo-based dual signal amplification biosensor for rapid and highly sensitive detection of alkaline phosphatase activity. Mikrochim Acta. 2024;191(7):439. https:\/\/doi.org\/10.1007\/s00604-024-06516-9.","journal-title":"Mikrochim Acta"},{"issue":"13","key":"6152_CR31","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab274","volume":"49","author":"Q Liu","year":"2021","unstructured":"Liu Q, Guo X, Xun G, Li Z, Chong Y, Yang L, et al. Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations. Nucleic Acids Res. 2021;49(13):e75. https:\/\/doi.org\/10.1093\/nar\/gkab274.","journal-title":"Nucleic Acids Res"},{"issue":"16","key":"6152_CR32","doi-asserted-by":"publisher","first-page":"3335","DOI":"10.1039\/d4cc06481j","volume":"61","author":"J Lin","year":"2025","unstructured":"Lin J, Zhang J, Zhu X, Xia X, Zhang Y, Zeng Q, et al. Pyrococcus furiosus argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens. Chem Commun (Camb). 2025;61(16):3335\u20138. https:\/\/doi.org\/10.1039\/d4cc06481j.","journal-title":"Chem Commun (Camb)"},{"issue":"49","key":"6152_CR33","doi-asserted-by":"publisher","first-page":"17151","DOI":"10.1021\/acs.analchem.2c03563","volume":"94","author":"C Zhao","year":"2022","unstructured":"Zhao C, Yang L, Zhang X, Tang Y, Wang Y, Shao X, et al. Rapid and sensitive genotyping of SARS-CoV-2 key mutation L452R with an RPA-PfAgo method. Anal Chem. 2022;94(49):17151\u20139. https:\/\/doi.org\/10.1021\/acs.analchem.2c03563.","journal-title":"Anal Chem"},{"issue":"1","key":"6152_CR34","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-024-06575-0","volume":"17","author":"L Chen","year":"2024","unstructured":"Chen L, Chen W, Wei H, Lin W, Zhang C, Hu H, et al. Rapid and supersensitive allele detection of Plasmodium falciparum chloroquine resistance via a Pyrococcus furiosus argonaute-triggered dual-signal biosensing platform. Parasit Vectors. 2024;17(1):488. https:\/\/doi.org\/10.1186\/s13071-024-06575-0.","journal-title":"Parasit Vectors"},{"key":"6152_CR35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2024.100568","volume":"26","author":"H Zhu","year":"2024","unstructured":"Zhu H, Zhu D, Li Y, Li Y, Song X, Mo J, et al. Rapid detection of mutations in the suspected piperaquine resistance gene E415G-exo in Plasmodium falciparum exonuclease via AS-PCR and RAA with CRISPR\/Cas12a. Int J Parasitol Drugs Drug Resist. 2024;26:100568. https:\/\/doi.org\/10.1016\/j.ijpddr.2024.100568.","journal-title":"Int J Parasitol Drugs Drug Resist"},{"issue":"2","key":"6152_CR36","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02719-21","volume":"10","author":"W Cheng","year":"2022","unstructured":"Cheng W, Song X, Zhu H, Wu K, Wang W, Li J. A rapid and specific genotyping platform for Plasmodium falciparum chloroquine resistance via allele-specific PCR with a lateral flow assay. Microbiol Spectr. 2022;10(2):e0271921. https:\/\/doi.org\/10.1128\/spectrum.02719-21.","journal-title":"Microbiol Spectr"},{"issue":"8","key":"6152_CR37","doi-asserted-by":"publisher","first-page":"4678","DOI":"10.1021\/acs.analchem.4c07109","volume":"97","author":"F Su","year":"2025","unstructured":"Su F, Zhao W, Zhao F, Cao M, Zhu T, Lv W, et al. Pyrococcus furiosus Argonaute-based fluorometric biosensor for one-tube detection of cancer-associated single nucleotide polymorphisms in microRNAs. Anal Chem. 2025;97(8):4678\u201386. https:\/\/doi.org\/10.1021\/acs.analchem.4c07109.","journal-title":"Anal Chem"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-06152-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-025-06152-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-025-06152-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T18:16:49Z","timestamp":1764181009000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-025-06152-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,30]]},"references-count":37,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["6152"],"URL":"https:\/\/doi.org\/10.1007\/s00216-025-06152-z","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,30]]},"assertion":[{"value":"20 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 September 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 October 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of the First Dongguan Affiliated Hospital, Guangdong Medical University (No. YSYJ202501028).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}