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Here, we evaluate six triarylpyridinium\u2013bodipy (TAPY\u2013BDP) conjugates as mitochondrial markers in\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and\n                    <jats:italic>Pichia kudriavzevii<\/jats:italic>\n                    (formerly\n                    <jats:italic>Candida krusei<\/jats:italic>\n                    ), using a triphenylphosphonium\u2013bodipy (TPP\u2013BDP) dyad and a non-targeted propyl\u2013bodipy (prop-BDP)\u00a0as references. Confocal microscopy and flow cytometry quantified uptake and localization. Colocalization with MitoTracker\u2122 Deep Red gave Pearson correlation coefficients up to 0.9, supporting efficient targeting for most TAPY derivatives. TAPY\u2013BDPs with R\u2009=\u2009H, Me, OMe, or NMe\n                    <jats:sub>2<\/jats:sub>\n                    showed higher mitochondrial fluorescence than the more lipophilic analogues (R\u2009=\u2009Cl, CF\n                    <jats:sub>3<\/jats:sub>\n                    ) and the TPP\u2013BDP reference at 500\u00a0nM, consistent with flow cytometric analysis. Notably, TAPY(OMe)\u2013BDP maintained robust mitochondrial staining at 100\u00a0nM in both fungi, whereas TPP\u2013BDP decreased to near-background. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) reduced fluorescence for all cationic probes, confirming mitochondrial\u00a0membrane potential\u2013driven accumulation. Overall,\u00a0these results indicate that TAPY is a competitive alternative to TPP for mitochondrial vectorization in fungi, expanding probe options for bioanalytical studies in azole-resistant yeasts and potentially enabling mitochondria-directed therapeutic delivery.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s00216-026-06404-6","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T13:47:29Z","timestamp":1772113649000},"page":"2745-2756","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fluorescent TAPY\u2013bodipy dyads as tools for imaging fungal mitochondria by confocal microscopy and flow cytometry"],"prefix":"10.1007","volume":"418","author":[{"given":"Jean C.","family":"Neto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"de Llanos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0826-6084","authenticated-orcid":false,"given":"Francisco","family":"Galindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"issue":"23","key":"6404_CR1","doi-asserted-by":"publisher","first-page":"14276","DOI":"10.1021\/acs.jmedchem.0c01013","volume":"63","author":"Y Xu","year":"2020","unstructured":"Xu Y, Xue D, Bankhead A, Neamati N. 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