{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:08:11Z","timestamp":1792782491422,"version":"4.5.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"20","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"The Danish Council for Independent Research|Medical Sciences","award":["DFF \u2013 4002-00391"],"award-info":[{"award-number":["DFF \u2013 4002-00391"]}],"id":[{"id":"https:\/\/ror.org\/05svhj534","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002808","name":"Carlsbergfondet","doi-asserted-by":"publisher","award":["CF14-0214"],"award-info":[{"award-number":["CF14-0214"]}],"id":[{"id":"10.13039\/501100002808","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Odontology, University of Copenhagen","id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Copenhagen University","id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1007\/s00216-026-06515-0","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T21:51:20Z","timestamp":1776981080000},"page":"6657-6667","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In situ MALDI mass spectrometry imaging of pharmaceuticals and metabolites in human teeth and jawbone"],"prefix":"10.1007","volume":"418","author":[{"given":"Hameda","family":"Amin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Daniel Brandbjerg Bohn","family":"Lorensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald Severin","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azam","family":"Bakhshandeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kofod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"Werner Moeller Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla","family":"Kragelund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Janfelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,23]]},"reference":[{"key":"6515_CR1","unstructured":"Nanci A.\u00a0Ten cate's oral histology: development, structure, and function. 10th ed. Elsevier; 2024."},{"issue":"10","key":"6515_CR2","doi-asserted-by":"publisher","first-page":"2675","DOI":"10.1007\/s00204-023-03573-6","volume":"97","author":"M Klima","year":"2023","unstructured":"Klima M, Auw\u00e4rter V, Altenburger MJ, Neukamm MA. In vitro studies on the dependence of drug deposition in dentin on drug concentration, contact time, and the physicochemical properties of the drugs. Arch Toxicol. 2023;97(10):2675\u201385.","journal-title":"Arch Toxicol"},{"key":"6515_CR3","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.talanta.2017.08.047","volume":"176","author":"K Henkel","year":"2018","unstructured":"Henkel K, Altenburger MJ, Auw\u00e4rter V, Neukamm MA. Full validation of a method for the determination of drugs of abuse in non-mineralized dental biofilm using liquid chromatography-tandem mass spectrometry and application to postmortem samples. Talanta. 2018;176:360\u20136.","journal-title":"Talanta"},{"key":"6515_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e23177","author":"A Riedel","year":"2024","unstructured":"Riedel A, Neukamm MA, Klima M, Henkel K, Auw\u00e4rter V, Altenburger MJ. Drugs in dental biofilm and enamel\u2013a pilot study. Heliyon. 2024. https:\/\/doi.org\/10.1016\/j.heliyon.2023.e23177.","journal-title":"Heliyon"},{"key":"6515_CR5","doi-asserted-by":"crossref","unstructured":"Coulter CA, Moore CM. Analysis of drugs in oral fluid using LC-MS\/MS. In: LC-MS in Drug Analysis: Methods and Protocols. Springer; 2018. p. 237\u201359.","DOI":"10.1007\/978-1-4939-8823-5_22"},{"issue":"2","key":"6515_CR6","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.forsciint.2007.03.034","volume":"170","author":"M Concheiro","year":"2007","unstructured":"Concheiro M, de Castro A, Quintela \u00d3, Cruz A, L\u00f3pez-Rivadulla M. Confirmation by LC\u2013MS of drugs in oral fluid obtained from roadside testing. Forensic Sci Int. 2007;170(2):156\u201362.","journal-title":"Forensic Sci Int"},{"key":"6515_CR7","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1016\/j.forsciint.2016.02.021","volume":"265","author":"M Klima","year":"2016","unstructured":"Klima M, Altenburger MJ, Kempf J, Auw\u00e4rter V, Neukamm MA. Determination of medicinal and illicit drugs in post mortem dental hard tissues and comparison with analytical results for body fluids and hair samples. Forensic Sci Int. 2016;265:166\u201371.","journal-title":"Forensic Sci Int"},{"key":"6515_CR8","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2024.112185","volume":"363","author":"I Bianchi","year":"2024","unstructured":"Bianchi I, Cippitelli M, Buratti E, Cerioni A, Mietti G, Focardi M, et al. A pilot study on post-mortem determination of drug abuse on dental tissues. Forensic Sci Int. 2024;363:112185.","journal-title":"Forensic Sci Int"},{"issue":"1","key":"6515_CR9","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1093\/jat\/bkw105","volume":"41","author":"G Ottaviani","year":"2017","unstructured":"Ottaviani G, Cameriere R, Cippitelli M, Froldi R, Tassoni G, Zampi M, et al. Determination of drugs of abuse in a single sample of human teeth by a gas chromatography-mass spectrometry method. J Anal Toxicol. 2017;41(1):32\u20136.","journal-title":"J Anal Toxicol"},{"issue":"3","key":"6515_CR10","doi-asserted-by":"publisher","first-page":"310","DOI":"10.15644\/asc59\/3\/8","volume":"59","author":"I Bianchi","year":"2025","unstructured":"Bianchi I, Croce E, Gelli F, Dimitrova A, Pradella F, Defraia B, et al. A pilot study on the deposition of drugs in dental tissues as alternative matrices for forensic toxicology. Acta stomatologica Croatica: International journal of oral sciences and dental medicine. 2025;59(3):310\u201321.","journal-title":"Acta stomatologica Croatica: International journal of oral sciences and dental medicine"},{"issue":"5","key":"6515_CR11","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/j.bone.2005.05.003","volume":"38","author":"GH Nancollas","year":"2006","unstructured":"Nancollas GH, Tang R, Phipps RJ, Henneman Z, Gulde S, Wu W, et al. Novel insights into actions of bisphosphonates on bone: differences in interactions with hydroxyapatite. Bone. 2006;38(5):617\u201327.","journal-title":"Bone"},{"key":"6515_CR12","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1039\/C3NP70100J","volume":"31","author":"N Bjarnholt","year":"2014","unstructured":"Bjarnholt N, Li B, D\u2019Alvise J, Janfelt C. Mass spectrometry imaging of plant metabolites \u2013 principles and possibilities. Nat Prod Rep. 2014;31:818\u201337.","journal-title":"Nat Prod Rep"},{"key":"6515_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2021.116482","volume":"146","author":"JR Granborg","year":"2022","unstructured":"Granborg JR, Handler AM, Janfelt C. Mass spectrometry imaging in drug distribution and drug metabolism studies \u2013 principles, applications and perspectives. TrAC Trends Anal Chem. 2022;146:116482.","journal-title":"TrAC Trends Anal Chem"},{"issue":"29","key":"6515_CR14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2114365119","volume":"119","author":"T Bien","year":"2022","unstructured":"Bien T, Koerfer K, Schwenzfeier J, Dreisewerd K, Soltwisch J. Mass spectrometry imaging to explore molecular heterogeneity in cell culture. Proc Natl Acad Sci U S A. 2022;119(29):e2114365119.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"15","key":"6515_CR15","doi-asserted-by":"publisher","first-page":"2690","DOI":"10.1039\/C7AN00565B","volume":"142","author":"P-M Vaysse","year":"2017","unstructured":"Vaysse P-M, Heeren RMA, Porta T, Balluff B. Mass spectrometry imaging for clinical research \u2013 latest developments, applications, and current limitations. Analyst. 2017;142(15):2690\u2013712.","journal-title":"Analyst"},{"issue":"11","key":"6515_CR16","doi-asserted-by":"publisher","first-page":"7630","DOI":"10.1021\/acs.analchem.0c00359","volume":"92","author":"M Colley","year":"2020","unstructured":"Colley M, Liang S, Tan C, Trobough KP, Bach SBH, Chun Y-H. Mapping and identification of native proteins of developing teeth in mouse mandibles. Anal Chem. 2020;92(11):7630\u20137.","journal-title":"Anal Chem"},{"issue":"8","key":"6515_CR17","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9486","volume":"37","author":"J Dekker","year":"2023","unstructured":"Dekker J, Larson T, Tzvetkov J, Harvey VL, Dowle A, Hagan R, et al. Spatial analysis of the ancient proteome of archeological teeth using mass spectrometry imaging. Rapid Commun Mass Spectrom. 2023;37(8):e9486.","journal-title":"Rapid Commun Mass Spectrom"},{"key":"6515_CR18","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/j.microc.2012.09.005","volume":"106","author":"J Farell","year":"2013","unstructured":"Farell J, Amarasiriwardena D, Goodman AH, Arriaza B. Bioimaging of trace metals in ancient Chilean mummies and contemporary Egyptian teeth by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Microchem J. 2013;106:340\u20136.","journal-title":"Microchem J"},{"issue":"5","key":"6515_CR19","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/j.jdent.2011.03.004","volume":"39","author":"D Hare","year":"2011","unstructured":"Hare D, Austin C, Doble P, Arora M. Elemental bio-imaging of trace elements in teeth using laser ablation-inductively coupled plasma-mass spectrometry. J Dent. 2011;39(5):397\u2013403.","journal-title":"J Dent"},{"issue":"5","key":"6515_CR20","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1007\/s00216-013-7075-y","volume":"406","author":"H Hirano","year":"2014","unstructured":"Hirano H, Masaki N, Hayasaka T, Watanabe Y, Masumoto K, Nagata T, et al. Matrix-assisted laser desorption\/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure. Anal Bioanal Chem. 2014;406(5):1355\u201363.","journal-title":"Anal Bioanal Chem"},{"issue":"1","key":"6515_CR21","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.bone.2007.08.037","volume":"42","author":"V Balter","year":"2008","unstructured":"Balter V, Reynard B. Secondary ionization mass spectrometry imaging of dilute stable strontium labeling in dentin and enamel. Bone. 2008;42(1):229\u201334.","journal-title":"Bone"},{"issue":"6","key":"6515_CR22","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1039\/D0JA00045K","volume":"35","author":"M Edinborough","year":"2020","unstructured":"Edinborough M, Pilgrim M, Fearn S, Cvetkovi\u0107 A, Mihailovi\u0107 B, Grbi\u0107 R, et al. Mineralisation within human tooth cementum identified by secondary ion mass spectrometry. J Anal At Spectrom. 2020;35(6):1199\u2013206.","journal-title":"J Anal At Spectrom"},{"issue":"3","key":"6515_CR23","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1080\/17425247.2017.1260119","volume":"14","author":"E Wenande","year":"2017","unstructured":"Wenande E, Olesen UH, Nielsen MM, Janfelt C, Hansen SH, Anderson RR, et al. Fractional laser-assisted topical delivery leads to enhanced, accelerated and deeper cutaneous 5-fluorouracil uptake. Expert Opin Drug Deliv. 2017;14(3):307\u201317.","journal-title":"Expert Opin Drug Deliv"},{"issue":"1","key":"6515_CR24","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/s13361-017-1809-6","volume":"29","author":"MT Bokhart","year":"2018","unstructured":"Bokhart MT, Nazari M, Garrard KP, Muddiman DC. MSiReader v1.0: evolving open-source mass spectrometry imaging software for targeted and untargeted analyses. J Am Soc Mass Spectrom. 2018;29(1):8\u201316.","journal-title":"J Am Soc Mass Spectrom"},{"issue":"1","key":"6515_CR25","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/nmeth.4072","volume":"14","author":"A Palmer","year":"2017","unstructured":"Palmer A, Phapale P, Chernyavsky I, Lavigne R, Fay D, Tarasov A, et al. FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry. Nat Methods. 2017;14(1):57\u201360.","journal-title":"Nat Methods"},{"issue":"9","key":"6515_CR26","doi-asserted-by":"publisher","first-page":"3256","DOI":"10.1021\/ac2004967","volume":"83","author":"J Thunig","year":"2011","unstructured":"Thunig J, Hansen SH, Janfelt C. Analysis of secondary plant metabolites by indirect desorption electrospray ionization imaging mass spectrometry. Anal Chem. 2011;83(9):3256\u20139.","journal-title":"Anal Chem"},{"issue":"10","key":"6515_CR27","doi-asserted-by":"publisher","first-page":"2683","DOI":"10.1007\/s00216-020-02920-1","volume":"413","author":"M Vandenbosch","year":"2021","unstructured":"Vandenbosch M, Nauta SP, Svirkova A, Poeze M, Heeren RM, Siegel TP, et al. Sample preparation of bone tissue for MALDI-MSI for forensic and (pre) clinical applications. Anal Bioanal Chem. 2021;413(10):2683\u201394.","journal-title":"Anal Bioanal Chem"},{"key":"6515_CR28","doi-asserted-by":"publisher","first-page":"1634","DOI":"10.1002\/pmic.201500422","volume":"16","author":"S Okutan","year":"2016","unstructured":"Okutan S, Hansen HS, Janfelt C. A simplified approach to cryo-sectioning of mice for whole-body imaging of drugs and metabolites with desorption electrospray ionization mass spectrometry imaging. Proteomics. 2016;16:1634\u201341.","journal-title":"Proteomics"},{"issue":"1-4","key":"6515_CR29","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1159\/000151454","volume":"189","author":"WJ Landis","year":"2008","unstructured":"Landis WJ, Silver FH. Mineral deposition in the extracellular matrices of vertebrate tissues: identification of possible apatite nucleation sites on type I collagen. Cells Tissues Organs. 2008;189(1\u20134):20\u20134.","journal-title":"Cells Tissues Organs"},{"issue":"4","key":"6515_CR30","first-page":"105","volume":"8","author":"Y Lee","year":"2017","unstructured":"Lee Y, Seo E, Park T-M, Bae K-H, Cha S. Analysis of lipids in deciduous teeth by matrix-assisted laser desorption\/ionization mass spectrometry (MALDI MS). Mass Spectrometry Letters. 2017;8(4):105\u20138.","journal-title":"Mass Spectrometry Letters"},{"issue":"11","key":"6515_CR31","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12111096","volume":"12","author":"AL Sohn","year":"2022","unstructured":"Sohn AL, Ping L, Glass JD, Seyfried NT, Hector EC, Muddiman DC. Interrogating the metabolomic profile of amyotrophic lateral sclerosis in the post-mortem human brain by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI). Metabolites. 2022;12(11):1096.","journal-title":"Metabolites"},{"issue":"3","key":"6515_CR32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jebdp.2020.101470","volume":"20","author":"MN Bijle","year":"2020","unstructured":"Bijle MN, Ekambaram M, Yiu CKY. A scoping review on arginine in caries prevention. J Evid Based Dent Pract. 2020;20(3):101470.","journal-title":"J Evid Based Dent Pract"},{"issue":"1-3","key":"6515_CR33","doi-asserted-by":"publisher","first-page":"105","DOI":"10.3109\/03008209509016989","volume":"33","author":"M Goldberg","year":"1995","unstructured":"Goldberg M, Septier D, Lecolle S, Vermelin L, Bissila-Mapahou R, Carreau J, et al. Lipids in predentine and dentine. Connect Tissue Res. 1995;33(1\u20133):105\u201314.","journal-title":"Connect Tissue Res"},{"issue":"9","key":"6515_CR34","first-page":"649","volume":"75","author":"AS Gaffen","year":"2009","unstructured":"Gaffen AS, Haas DA. Survey of local anesthetic use by Ontario dentists. J Can Dent Assoc. 2009;75(9):649.","journal-title":"J Can Dent Assoc"},{"issue":"1","key":"6515_CR35","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-84756-8","volume":"15","author":"PJ Chen","year":"2025","unstructured":"Chen PJ, Wang K, Lin MH, Sharma R, Kalajzic Z, O\u2019Brien M, et al. Alendronate partially rescues the periodontal defects in OIM mouse model of osteogenesis imperfecta. Sci Rep. 2025;15(1):88.","journal-title":"Sci Rep"},{"key":"6515_CR36","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.jchromb.2011.11.031","volume":"881-882","author":"S Ghassabian","year":"2012","unstructured":"Ghassabian S, Wright LA, deJager AD, Smith MT. Development and validation of a sensitive solid-phase-extraction (SPE) method using high-performance liquid chromatography\/tandem mass spectrometry (LC\u2013MS\/MS) for determination of risedronate concentrations in human plasma. J Chromatogr B. 2012;881\u2013882:34\u201341.","journal-title":"J Chromatogr B"},{"key":"6515_CR37","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.jchromb.2013.07.019","volume":"935","author":"BS Raccor","year":"2013","unstructured":"Raccor BS, Sun J, Lawrence RF, Li L, Zhang H, Somerman MJ, et al. Quantitation of zoledronic acid in murine bone by liquid chromatography coupled with tandem mass spectrometry. J Chromatogr B. 2013;935:54\u201360.","journal-title":"J Chromatogr B"},{"issue":"7","key":"6515_CR38","doi-asserted-by":"publisher","DOI":"10.1002\/jms.5148","volume":"60","author":"MM Rahman","year":"2025","unstructured":"Rahman MM, Afroz MS, Mamun MA, Islam A, Sakamoto T, Aramaki S, et al. Quantitative mass spectrometry imaging by targeted-DESI-MSI in MRM mode provides higher sensitivity and specificity for fast quantification of chloroquine drug in mice kidney. J Mass Spectrom. 2025;60(7):e5148.","journal-title":"J Mass Spectrom"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-026-06515-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-026-06515-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-026-06515-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:03:00Z","timestamp":1790622180000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-026-06515-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,23]]},"references-count":38,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["6515"],"URL":"https:\/\/doi.org\/10.1007\/s00216-026-06515-0","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,23]]},"assertion":[{"value":"28 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 April 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 April 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study was approved by The Danish Data Protection Agency (P-2022-856) and the Regional Scientific Ethical Committee.","order":1,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflicts of interest.","order":2,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Raw data from the study in the form of imzML files have been uploaded to the METASPACE annotation platform from where they can be freely accessed via the following link:","order":3,"name":"Ethics","label":"Associated content","group":{"name":"EthicsHeading","label":"Declarations"}}]}}