{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:59:46Z","timestamp":1790686786408,"version":"4.1.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274092"],"award-info":[{"award-number":["22274092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Anal Bioanal Chem"],"DOI":"10.1007\/s00216-026-06850-2","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:20:00Z","timestamp":1790684400000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fluorescence biosensing for simultaneous detection of HPV16 and HPV18"],"prefix":"10.1007","author":[{"given":"Ze-Lin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao-Li","family":"Mou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Liang","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Ying","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Qi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"6850_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.esmoop.2021.100154","volume":"6","author":"L Cabel","year":"2021","unstructured":"Cabel L, Bonneau C, Bernard-Tessier A, Hequet D, Tran-Perennou C, Bataillon G, et al. HPV ctDNA detection of high-risk HPV types during chemoradiotherapy for locally advanced cervical cancer. ESMO Open. 2021;6:100154. https:\/\/doi.org\/10.1016\/j.esmoop.2021.100154.","journal-title":"ESMO Open"},{"key":"6850_CR2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13080653","volume":"13","author":"EA Flores-Contreras","year":"2024","unstructured":"Flores-Contreras EA, Gonzalez-Gonzalez E, Trujillo-Rodriguez GJ, Rodriguez-Sanchez IP, Ancer-Rodriguez J, Perez-Maya AA, et al. Isothermal technologies for HPV detection: current trends and future perspectives. Pathogens. 2024;13:653. https:\/\/doi.org\/10.3390\/pathogens13080653.","journal-title":"Pathogens"},{"key":"6850_CR3","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1001\/jama.2023.13174","volume":"330","author":"RB Perkins","year":"2023","unstructured":"Perkins RB, Wentzensen N, Guido RS, Schiffman M. Cervical cancer screening: a review. J Am Med Assoc. 2023;330:547\u201358. https:\/\/doi.org\/10.1001\/jama.2023.13174.","journal-title":"J Am Med Assoc"},{"key":"6850_CR4","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1016\/S0140-6736(13)60022-7","volume":"382","author":"EJ Crosbie","year":"2013","unstructured":"Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet. 2013;382:889\u201399. https:\/\/doi.org\/10.1016\/S0140-6736(13)60022-7.","journal-title":"Lancet"},{"key":"6850_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2020.100435","volume":"23","author":"DM Harper","year":"2020","unstructured":"Harper DM, Sheinfeld Gorin S. HPV vaccination bridges to HPV screening. EClinicalMedicine. 2020;23:100435. https:\/\/doi.org\/10.1016\/j.eclinm.2020.100435.","journal-title":"EClinicalMedicine"},{"key":"6850_CR6","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1459572","volume":"14","author":"Y Shao","year":"2024","unstructured":"Shao Y, Shah PT, Su Q, Li S, Huang F, Wang J, et al. Recombinant adenoviruses expressing HPV16\/18 E7 upregulate the HDAC6 and DNMT3B genes in C33A cells. Front Cell Infect Microbiol. 2024;14:1459572. https:\/\/doi.org\/10.3389\/fcimb.2024.1459572.","journal-title":"Front Cell Infect Microbiol"},{"key":"6850_CR7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-13423-3","volume":"15","author":"A Lahdhiri","year":"2025","unstructured":"Lahdhiri A, Benzina B, Ennaifer E, Tounsi H, Gzara A, Rammeh-Rommani S, et al. HPV DNA screening and vaccination strategies in Tunisia. Sci Rep. 2025;15:27916. https:\/\/doi.org\/10.1038\/s41598-025-13423-3.","journal-title":"Sci Rep"},{"key":"6850_CR8","doi-asserted-by":"publisher","first-page":"3793","DOI":"10.1002\/jmv.25962","volume":"92","author":"X Ma","year":"2020","unstructured":"Ma X, Li Y, Liu R, Wei W, Ding C. Development of a sensitive and specific nanoparticle-assisted PCR assay for detecting HPV-16 and HPV-18 DNA. J Med Virol. 2020;92:3793\u20138. https:\/\/doi.org\/10.1002\/jmv.25962.","journal-title":"J Med Virol"},{"key":"6850_CR9","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.jviromet.2019.04.023","volume":"270","author":"M Daskou","year":"2019","unstructured":"Daskou M, Tsakogiannis D, Dimitriou TG, Amoutzias GD, Mossialos D, Kottaridi C, et al. WarmStart colorimetric LAMP for the specific and rapid detection of HPV16 and HPV18 DNA. J Virol Methods. 2019;270:87\u201394. https:\/\/doi.org\/10.1016\/j.jviromet.2019.04.023.","journal-title":"J Virol Methods"},{"key":"6850_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.121015","volume":"217","author":"Z Fan","year":"2020","unstructured":"Fan Z, Feng X, Zhang W, Li N, Zhang X, Lin JM. Visual detection of high-risk HPV16 and HPV18 based on loop-mediated isothermal amplification. Talanta. 2020;217:121015. https:\/\/doi.org\/10.1016\/j.talanta.2020.121015.","journal-title":"Talanta"},{"key":"6850_CR11","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.jcv.2016.12.004","volume":"87","author":"DA Cook","year":"2017","unstructured":"Cook DA, Smith LW, Law J, Mei W, van Niekerk DJ, Ceballos K, et al. Aptima HPV Assay versus Hybrid Capture((R)) 2 HPV test for primary cervical cancer screening in the HPV FOCAL trial. J Clin Virol. 2017;87:23\u20139. https:\/\/doi.org\/10.1016\/j.jcv.2016.12.004.","journal-title":"J Clin Virol"},{"key":"6850_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117831","volume":"288","author":"Y Long","year":"2025","unstructured":"Long Y, Yu T, Shi D, Song L, Liu G. T7-assisted special rolling circular amplification platform for point-of-care cervical cancer screening. Biosens Bioelectron. 2025;288:117831. https:\/\/doi.org\/10.1016\/j.bios.2025.117831.","journal-title":"Biosens Bioelectron"},{"key":"6850_CR13","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1016\/j.snb.2019.01.158","volume":"286","author":"X Peng","year":"2019","unstructured":"Peng X, Zhang Y, Lu D, Guo Y, Guo S. Ultrathin Ti3C2 nanosheets based \u201coff-on\u201d fluorescent nanoprobe for rapid and sensitive detection of HPV infection. Sens Actuators B Chem. 2019;286:222\u20139. https:\/\/doi.org\/10.1016\/j.snb.2019.01.158.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR14","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.talanta.2014.05.011","volume":"128","author":"W Wang","year":"2014","unstructured":"Wang W, Pang DW, Tang HW. Sensitive multiplexed DNA detection using silica nanoparticles as the target capturing platform. Talanta. 2014;128:263\u20137. https:\/\/doi.org\/10.1016\/j.talanta.2014.05.011.","journal-title":"Talanta"},{"key":"6850_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2023.135128","volume":"403","author":"X Jiang","year":"2024","unstructured":"Jiang X, Yin C, Wu M, Cao S, Niu N, Chen L. Fluorescent switch based on QDs modified DNA probe and NiTPP for simultaneous dual color sensitive sensing of HPV16 and HPV18. Sens Actuators B Chem. 2024;403:135128. https:\/\/doi.org\/10.1016\/j.snb.2023.135128.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR16","doi-asserted-by":"publisher","first-page":"10605","DOI":"10.1039\/c3cc45459b","volume":"49","author":"S Persano","year":"2013","unstructured":"Persano S, Valentini P, Kim JH, Pompa PP. Colorimetric detection of human papilloma virus by double isothermal amplification. Chem Commun (Camb). 2013;49:10605\u20137. https:\/\/doi.org\/10.1039\/c3cc45459b.","journal-title":"Chem Commun (Camb)"},{"key":"6850_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2025.129166","volume":"300","author":"M Xi","year":"2026","unstructured":"Xi M, Li Q, Chen X, Fei S, Zhang K, Deng Z, et al. Cobalt-ferrocene MOF-based colorimetric biosensing platform for naked-eye detection of human papillomavirus. Talanta. 2026;300:129166. https:\/\/doi.org\/10.1016\/j.talanta.2025.129166.","journal-title":"Talanta"},{"key":"6850_CR18","doi-asserted-by":"publisher","first-page":"10353","DOI":"10.1021\/acs.analchem.5c00655","volume":"97","author":"Y Liu","year":"2025","unstructured":"Liu Y, Liang H, Yuan R, Yang X. PER-based DNA cube captor for high specific and efficient SERS detection of HPV. Anal Chem. 2025;97:10353\u20139. https:\/\/doi.org\/10.1021\/acs.analchem.5c00655.","journal-title":"Anal Chem"},{"key":"6850_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.118239","volume":"294","author":"X Chen","year":"2026","unstructured":"Chen X, Chen Y, Tang W, Xu J, Zhu Z, Hu Q, et al. Enzyme-linked lectin assay for amplified electrochemical detection of HPV DNA. Biosens Bioelectron. 2026;294:118239. https:\/\/doi.org\/10.1016\/j.bios.2025.118239.","journal-title":"Biosens Bioelectron"},{"key":"6850_CR20","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1021\/acsami.0c20742","volume":"13","author":"Y He","year":"2021","unstructured":"He Y, Liu Y, Cheng L, Yang Y, Qiu B, Guo L, et al. Highly reproducible and sensitive electrochemiluminescence biosensors for HPV detection based on bovine serum albumin carrier platforms and hyperbranched rolling circle amplification. ACS Appl Mater Interfaces. 2021;13:298\u2013305. https:\/\/doi.org\/10.1021\/acsami.0c20742.","journal-title":"ACS Appl Mater Interfaces"},{"key":"6850_CR21","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1007\/s00604-022-05283-9","volume":"189","author":"HH Zhu","year":"2022","unstructured":"Zhu HH, Li Y, Wu LX, Wang KS, Zhang Y, Fan QY, et al. Internal heating method of loop-mediated isothermal amplification for detection of HPV-6 DNA. Microchim Acta. 2022;189:212. https:\/\/doi.org\/10.1007\/s00604-022-05283-9.","journal-title":"Microchim Acta"},{"key":"6850_CR22","doi-asserted-by":"publisher","first-page":"4281","DOI":"10.1039\/c7cs00055c","volume":"46","author":"S Bi","year":"2017","unstructured":"Bi S, Yue S, Zhang S. Hybridization chain reaction: a versatile molecular tool for biosensing, bioimaging, and biomedicine. Chem Soc Rev. 2017;46:4281\u201398. https:\/\/doi.org\/10.1039\/c7cs00055c.","journal-title":"Chem Soc Rev"},{"key":"6850_CR23","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1038\/35020524","volume":"406","author":"B Yurke","year":"2000","unstructured":"Yurke B, Turberfield AJ, Mills AP, Simmel FC, Neumann JL. A DNA-fuelled molecular machine made of DNA. Nature. 2000;406:605\u20138. https:\/\/doi.org\/10.1038\/35020524.","journal-title":"Nature"},{"key":"6850_CR24","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2025.137591","volume":"434","author":"Y Lan","year":"2025","unstructured":"Lan Y, Ma Y, Xu Y, Zhang Y, Deng R, Liu L, et al. A label-free fluorescent aptasensor for AFB1 and AFM1 based on the aptamer tailoring strategy and synergistic signal amplification of HCR and MoS2 nanosheets. Sens Actuators B Chem. 2025;434:137591. https:\/\/doi.org\/10.1016\/j.snb.2025.137591.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.135867","volume":"413","author":"Z Song","year":"2024","unstructured":"Song Z, Fan Y, Zeng S, Sun L. Target and acidic tumor microenvironment-induced in situ HCR for bispecific tumor cell imaging and therapy. Sens Actuators B Chem. 2024;413:135867. https:\/\/doi.org\/10.1016\/j.snb.2024.135867.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.137222","volume":"427","author":"M Cheng","year":"2025","unstructured":"Cheng M, Wang L, Jiang M, Bao Y, Fan M, Shen H, et al. Enzyme-free nano-sensor for high-sensitivity miRNA detection and tumor imaging based on multicolor silver nanoclusters pair and DNA-mediated CHA reactions. Sens Actuators B Chem. 2025;427:137222. https:\/\/doi.org\/10.1016\/j.snb.2024.137222.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR27","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.136259","volume":"418","author":"D Zhu","year":"2024","unstructured":"Zhu D, Hu Y, Xia Y, Wei Y, Lin S, Chao J, et al. Non-enzymatic aptazyme-driven autonomous DNA circuits coupled with three-junction CHA for amplified live-cell ATP imaging. Sens Actuators B Chem. 2024;418:136259. https:\/\/doi.org\/10.1016\/j.snb.2024.136259.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2023.133655","volume":"385","author":"Y Liu","year":"2023","unstructured":"Liu Y, Shi Y, Ding K, Liu J, Wang H, Zhou H. Enhanced fluorescent detection of nucleic acid based on hairpin DNA-assisted toehold-mediated strand displacement reaction. Sens Actuators B Chem. 2023;385:133655. https:\/\/doi.org\/10.1016\/j.snb.2023.133655.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.136751","volume":"423","author":"DSS Marpaung","year":"2025","unstructured":"Marpaung DSS, Jiang SS, Fang W-T, Liao Y-C, Chuang M-C. Activated single nucleotide mismatch determination through toehold-embedded hairpin-mediated strand displacement reaction alongside CRISPR-Cas12a for detection of TP53 point mutation. Sens Actuators B Chem. 2025;423:136751. https:\/\/doi.org\/10.1016\/j.snb.2024.136751.","journal-title":"Sens Actuators B Chem"},{"key":"6850_CR30","doi-asserted-by":"publisher","first-page":"8602","DOI":"10.1039\/d5ay01184a","volume":"17","author":"ZL Wang","year":"2025","unstructured":"Wang ZL, Yang C, Sun JY, Zhan HY, Zhang ZQ. A dual-toehold-initiated strand displacement reaction-based fluorescent sensor for HIV DNA detection. Anal Methods. 2025;17:8602\u20139. https:\/\/doi.org\/10.1039\/d5ay01184a.","journal-title":"Anal Methods"},{"key":"6850_CR31","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2022.123554","volume":"247","author":"J Chen","year":"2022","unstructured":"Chen J, Wang M, Zhou C, Zhang J, Su X. Label-free and dual-mode biosensor for HPV DNA based on DNA\/silver nanoclusters and G-quadruplex\/hemin DNAzyme. Talanta. 2022;247:123554. https:\/\/doi.org\/10.1016\/j.talanta.2022.123554.","journal-title":"Talanta"},{"key":"6850_CR32","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1007\/s40242-019-9085-6","volume":"35","author":"Y Yuan","year":"2019","unstructured":"Yuan Y, Li S, Luo L, Wang Q, Fang H, Huang J, et al. DNA-silver nanocluster binary probes for ratiometric fluorescent detection of HPV-related DNA. Chem Res Chin Univ. 2019;35:581\u20135. https:\/\/doi.org\/10.1007\/s40242-019-9085-6.","journal-title":"Chem Res Chin Univ"}],"container-title":["Analytical and Bioanalytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-026-06850-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00216-026-06850-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00216-026-06850-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:20:01Z","timestamp":1790684401000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00216-026-06850-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":32,"alternative-id":["6850"],"URL":"https:\/\/doi.org\/10.1007\/s00216-026-06850-2","relation":{},"ISSN":["1618-2642","1618-2650"],"issn-type":[{"value":"1618-2642","type":"print"},{"value":"1618-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"7 July 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 September 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 September 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The Academic Committee of Shaanxi Normal University approved the use of human serum samples (No. 202610089).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}