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Because no method for determination of muscle mass was available for large cohort studies of aging men and women, lean body mass determined by dual X-ray absorptiometry or bioelectrical impedance was used as a surrogate measure of muscle mass. The data from these studies showed either no or a poor relationship between LBM and functional capacity and health related outcomes, leading to the conclusion of many that the amount of muscle may not be associated with these age-associated outcomes. It was assumed that some undefined index of muscle quality is the critical contributor. These studies also consistently showed that muscle strength is lost more quickly than lean mass. Total body muscle mass can now be measured directly, accurately and non-invasively using the D<jats:sub>3<\/jats:sub>creatine (D<jats:sub>3<\/jats:sub>Cr) dilution method. D<jats:sub>3<\/jats:sub>Cr muscle mass, but not DXA derived LBM, is strongly associated with functional capacity, falls and insulin resistance in older men and women. In addition, D<jats:sub>3<\/jats:sub>Cr muscle mass is associated with risk of disability, hip fracture and mortality. New and emerging data demonstrate that low muscle mass may serve as a diagnostic criterion for sarcopenia.<\/jats:p>","DOI":"10.1007\/s00223-023-01124-w","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T12:02:15Z","timestamp":1692360135000},"page":"3-8","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["D3Creatine Dilution as a Direct, Non-invasive and Accurate Measurement of Muscle Mass for Aging Research"],"prefix":"10.1007","volume":"114","author":[{"given":"William J.","family":"Evans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy M.","family":"Cawthon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"1124_CR1","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1093\/gerona\/50A.Special_Issue.5","volume":"50A","author":"W Evans","year":"1995","unstructured":"Evans W (1995) What is sarcopenia? 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