{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T13:23:43Z","timestamp":1766755423196,"version":"3.48.0"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Calcif Tissue Int"],"DOI":"10.1007\/s00223-025-01448-9","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T16:08:24Z","timestamp":1764605304000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Calcium-Based Phosphate Binder Induces Osteomalacia in a Rat Model of Adynamic Bone Disease"],"prefix":"10.1007","volume":"116","author":[{"given":"T\u00e2nia Priante","family":"de Oliveira Truyts","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Cunha","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia Rodrigues","family":"Neves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner Vasques","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosilene Motta","family":"Elias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Maria Affonso","family":"Moyses","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanda","family":"Jorgetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8476-6780","authenticated-orcid":false,"given":"Luciene Machado","family":"dos Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"1448_CR1","doi-asserted-by":"publisher","first-page":"100584","DOI":"10.1016\/j.xkme.2022.100584","volume":"5","author":"M Guedes","year":"2023","unstructured":"Guedes M, Bieber B, Dasgupta I, Vega A, Nitta K, Brunelli S, Hartman J, Raimann JG, Robinson BM, Pisoni RL (2023) Serum phosphorus level rises in US Hemodialysis patients over the past decade: a DOPPS special report. Kidney Med 5:100584","journal-title":"Kidney Med"},{"key":"1448_CR2","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1097\/MNH.0000000000001087","volume":"34","author":"V Pothula Venkata","year":"2025","unstructured":"Pothula Venkata V, Scialla JJ (2025) Momentum toward patient outcome trials in chronic kidney disease-mineral bone disorder. Curr Opin Nephrol Hypertens 34:304\u2013313","journal-title":"Curr Opin Nephrol Hypertens"},{"key":"1448_CR3","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1038\/ki.2013.213","volume":"84","author":"JL Finch","year":"2013","unstructured":"Finch JL, Lee DH, Liapis H, Ritter C, Zhang S, Suarez E, Ferder L, Slatopolsky E (2013) Phosphate restriction significantly reduces mortality in uremic rats with established vascular calcification. Kidney Int 84:1145\u20131153","journal-title":"Kidney Int"},{"key":"1448_CR4","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.1038\/sj.ki.5002241","volume":"71","author":"KR Neves","year":"2007","unstructured":"Neves KR, Graciolli FG, dos Reis LM, Graciolli RG, Neves CL, Magalhaes AO, Custodio MR, Batista DG, Jorgetti V, Moyses RM (2007) Vascular calcification: contribution of parathyroid hormone in renal failure. Kidney Int 71:1262\u20131270","journal-title":"Kidney Int"},{"key":"1448_CR5","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1007\/s00223-014-9929-5","volume":"96","author":"S Yamada","year":"2015","unstructured":"Yamada S, Tatsumoto N, Tokumoto M, Noguchi H, Ooboshi H, Kitazono T, Tsuruya K (2015) Phosphate binders prevent phosphate-induced cellular senescence of vascular smooth muscle cells and vascular calcification in a modified, adenine-based uremic rat model. Calcif Tissue Int 96:347\u2013358","journal-title":"Calcif Tissue Int"},{"key":"1448_CR6","doi-asserted-by":"crossref","unstructured":"Barrera-Baena P, Rodriguez-Garcia M, Rodriguez-Rubio E, Gonzalez-Llorente L, Ortiz A, Zoccali C, Locatelli F, Floege J, Cohen-Solal M, Ferreira MA, Ketteler M, London GM, Gorriz-Teruel JL, Sanchez-Alvarez E, Hevia-Suarez MA, Fernandez-Gomez JM, Martin-Carro B, Gomez-Alonso C, Alonso-Montes C, Cannata-Andia JB, Fernandez-Martin JL (2024) Cosmos Serum phosphate is associated with increased risk of bone fragility fractures in haemodialysis patients. Nephrol Dial Transplant 39:618\u2013626","DOI":"10.1093\/ndt\/gfad190"},{"key":"1448_CR7","doi-asserted-by":"publisher","first-page":"2237","DOI":"10.1111\/j.1523-1755.2004.66013.x","volume":"66","author":"KR Neves","year":"2004","unstructured":"Neves KR, Graciolli FG, dos Reis LM, Pasqualucci CA, Moyses RM, Jorgetti V (2004) Adverse effects of hyperphosphatemia on myocardial hypertrophy, renal function, and bone in rats with renal failure. Kidney Int 66:2237\u20132244","journal-title":"Kidney Int"},{"key":"1448_CR8","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/0019-2791(65)90004-2","volume":"2","author":"G Mancini","year":"1965","unstructured":"Mancini G, Carbonara AO, Heremans JF (1965) Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235\u2013254","journal-title":"Immunochemistry"},{"key":"1448_CR9","doi-asserted-by":"crossref","unstructured":"Duayer IF, Duque EJ, Fujihara CK, de Oliveira IB, Dos Reis LM, Machado FG, Graciolli FG, Jorgetti V, Zatz R, Elias RM, Moyses RMA (2021) The Protein-Independent role of phosphate in the progression of chronic kidney disease. Toxins (Basel) 13","DOI":"10.3390\/toxins13070503"},{"key":"1448_CR10","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1590\/S0100-879X2001000800007","volume":"34","author":"LH da Paz","year":"2001","unstructured":"da Paz LH, de Falco V, Teng NC, dos Reis LM, Pereira RM, Jorgetti V (2001) Effect of 17beta-estradiol or alendronate on the bone densitometry, bone histomorphometry and bone metabolism of ovariectomized rats. Braz J Med Biol Res 34:1015\u20131022","journal-title":"Braz J Med Biol Res"},{"key":"1448_CR11","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1002\/jbmr.1805","volume":"28","author":"DW Dempster","year":"2013","unstructured":"Dempster DW, Compston JE, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR, Parfitt AM (2013) Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR histomorphometry nomenclature committee. J Bone Min Res 28:2\u201317","journal-title":"J Bone Min Res"},{"key":"1448_CR12","doi-asserted-by":"publisher","first-page":"1436","DOI":"10.1016\/j.kint.2016.12.029","volume":"91","author":"FG Graciolli","year":"2017","unstructured":"Graciolli FG, Neves KR, Barreto F, Barreto DV, Dos Reis LM, Canziani ME, Sabbagh Y, Carvalho AB, Jorgetti V, Elias RM, Schiavi S, Moyses RMA (2017) The complexity of chronic kidney disease-mineral and bone disorder across stages of chronic kidney disease. Kidney Int 91:1436\u20131446","journal-title":"Kidney Int"},{"key":"1448_CR13","doi-asserted-by":"publisher","first-page":"153063","DOI":"10.1016\/j.phymed.2019.153063","volume":"64","author":"S Chai","year":"2019","unstructured":"Chai S, Wan L, Wang JL, Huang JC, Huang HX (2019) Gushukang inhibits osteocyte apoptosis and enhances BMP-2\/Smads signaling pathway in ovariectomized rats. Phytomedicine 64:153063","journal-title":"Phytomedicine"},{"key":"1448_CR14","doi-asserted-by":"publisher","first-page":"e79721","DOI":"10.1371\/journal.pone.0079721","volume":"8","author":"JC Ferreira","year":"2013","unstructured":"Ferreira JC, Ferrari GO, Neves KR, Cavallari RT, Dominguez WV, Dos Reis LM, Graciolli FG, Oliveira EC, Liu S, Sabbagh Y, Jorgetti V, Schiavi S, Moyses RM (2013) Effects of dietary phosphate on adynamic bone disease in rats with chronic kidney disease\u2013role of. sclerostin? PLoS One 8:e79721","journal-title":"sclerostin? PLoS One"},{"key":"1448_CR15","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1016\/j.ecl.2018.07.008","volume":"47","author":"MR Rubin","year":"2018","unstructured":"Rubin MR (2018) Skeletal manifestations of hypoparathyroidism. Endocrinol Metab Clin North Am 47:825\u2013837","journal-title":"Endocrinol Metab Clin North Am"},{"key":"1448_CR16","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.bone.2019.01.029","volume":"121","author":"GO Pires","year":"2019","unstructured":"Pires GO, Vieira IO, Hernandes FR, Teixeira AL, Oliveira IB, Dominguez WV, Dos Reis LM, Montenegro FM, Moyses RM, Carvalho AB, Jorgetti V (2019) Effects of parathyroidectomy on the biology of bone tissue in patients with chronic kidney disease and secondary hyperparathyroidism. Bone 121:277\u2013283","journal-title":"Bone"},{"key":"1448_CR17","doi-asserted-by":"publisher","first-page":"1904","DOI":"10.1093\/ndt\/gfh876","volume":"20","author":"Y Iwasaki-Ishizuka","year":"2005","unstructured":"Iwasaki-Ishizuka Y, Yamato H, Nii-Kono T, Kurokawa K, Fukagawa M (2005) Downregulation of parathyroid hormone receptor gene expression and osteoblastic dysfunction associated with skeletal resistance to parathyroid hormone in a rat model of renal failure with low turnover bone. Nephrol Dial Transpl 20:1904\u20131911","journal-title":"Nephrol Dial Transpl"},{"key":"1448_CR18","doi-asserted-by":"publisher","first-page":"1653","DOI":"10.1046\/j.1523-1755.2003.00284.x","volume":"64","author":"M Cozzolino","year":"2003","unstructured":"Cozzolino M, Staniforth ME, Liapis H, Finch J, Burke SK, Dusso AS, Slatopolsky E (2003) Sevelamer hydrochloride attenuates kidney and cardiovascular calcifications in long-term experimental uremia. Kidney Int 64:1653\u20131661","journal-title":"Kidney Int"},{"key":"1448_CR19","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1046\/j.1365-2362.1999.00499.x","volume":"29","author":"A Szabo","year":"1999","unstructured":"Szabo A, Freesmeyer MG, Abendroth K, Stein G, Rosivall L, El-Shakmak A, Ritz E (1999) Physiological doses of calcium regulatory hormones do not normalize bone cells in uraemic rats. Eur J Clin Invest 29:529\u2013535","journal-title":"Eur J Clin Invest"},{"key":"1448_CR20","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/8756-3282(90)90070-F","volume":"11","author":"WG Lieuallen","year":"1990","unstructured":"Lieuallen WG, Weisbrode SE, Horst RL, Nagode LA (1990) The effects of uremia and dietary phosphorus on the bone of rats. Bone 11:41\u201346","journal-title":"Bone"},{"key":"1448_CR21","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1081\/JDI-65423","volume":"27","author":"GJ Behets","year":"2005","unstructured":"Behets GJ, Gritters M, Dams G, De Broe ME, D\u2019Haese PC (2005) Effects of efficient phosphate binding on bone in chronic renal failure rats. Ren Fail 27:475\u2013484","journal-title":"Ren Fail"},{"key":"1448_CR22","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1002\/jcb.21001","volume":"100","author":"R Saunders","year":"2007","unstructured":"Saunders R, Szymczyk KH, Shapiro IM, Adams CS (2007) Matrix regulation of skeletal cell apoptosis III: mechanism of ion pair-induced apoptosis. J Cell Biochem 100:703\u2013715","journal-title":"J Cell Biochem"}],"container-title":["Calcified Tissue International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00223-025-01448-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00223-025-01448-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00223-025-01448-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T13:20:56Z","timestamp":1766755256000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00223-025-01448-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["1448"],"URL":"https:\/\/doi.org\/10.1007\/s00223-025-01448-9","relation":{},"ISSN":["1432-0827"],"issn-type":[{"type":"electronic","value":"1432-0827"}],"subject":[],"published":{"date-parts":[[2025,12,1]]},"assertion":[{"value":"7 August 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"T\u00e2nia P. O. M. Truyts, Juliana C. Ferreira, Katia R. Neves, Wagner V. Dominguez, Rosilene M. Elias and Luciene M. dos Reis have no conflict of interest to declare related to this study. Vanda Jorgetti has a grant provided by CNPq (National Council for Scientific and Technological Development). Rosa M.A. Moyses has a grant provided by CNPq, relationship with Inozyme Pharma that includes consulting or advisory; a relationship with Fresenius Medical Care and Baxter Hospital that includes speaking and lecture fees.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was carried out in accordance with the guidelines of the Ethics Committee for Analysis of Research Projects of the Hospital das Cl\u00ednicas and Faculdade de Medicina da Universidade de S\u00e3o Paulo (CAPPesq No. 371\/12) and the Ethics Committee on the Use of Animals (CEUA No. 1709\/2022).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}],"article-number":"139"}}