{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:28:29Z","timestamp":1786681709090,"version":"build-2736575974"},"reference-count":66,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000072","name":"NIDCR","doi-asserted-by":"crossref","award":["R01DE027076"],"award-info":[{"award-number":["R01DE027076"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["T32DE007306"],"award-info":[{"award-number":["T32DE007306"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Calcif Tissue Int"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Enamel, the outermost mineralized tissue of the tooth, is produced by specialized dental epithelial cells called ameloblasts. Unlike human enamel, which lacks regenerative capacity, the mouse incisor grows throughout life, driven by adult stem cells residing in the labial cervical loop (LaCl). To maintain tissue homeostasis, dental epithelial stem cells produce transit-amplifying cells (TACs) that commit to preameloblasts (PABs), migrate distally, and differentiate into enamel-forming ameloblasts. The full dental epithelial differentiation trajectory coexists within a single mouse incisor, making it an accessible model for studying adult tissue repair and regeneration. We have shown that the genome organizer SATB1 is enriched in PABs and is required for their differentiation into ameloblasts. Here, we investigated the injury response of PABs following mouse incisor tip trimming. Injured wild-type (\n                    <jats:italic>wt)<\/jats:italic>\n                    incisors exhibited an expanded PAB zone with intensive proliferation, reduced SATB1 in the ameloblast lineage, associated with a spatial delay in the deposition of the dentin\/enamel matrix compared to uninjured controls. Trimming of\n                    <jats:italic>Satb1 cKO<\/jats:italic>\n                    mouse incisor failed to elicit this response, highlighting SATB1\u2019s role in PAB\u2019s response to injury. Compared with\n                    <jats:italic>wt<\/jats:italic>\n                    controls, injured\n                    <jats:italic>wt<\/jats:italic>\n                    incisors and both\n                    <jats:italic>Satb1 cKO<\/jats:italic>\n                    groups showed increased Ki67 immunoreactivity in LaCl mesenchymal and epithelial compartments, along with reduced\n                    <jats:italic>Col1a1<\/jats:italic>\n                    expression in PAB microenvironment. In vitro, SATB1-transduced ameloblast lineage cells (ALCs) cultured on increasing concentrations of type I collagen exhibited reduced Ki67 but elevated\n                    <jats:italic>Amelx\/Ambn<\/jats:italic>\n                    expression. There findings indicate that SATB1 is required for epithelial TACs to exit the cell cycle and transition toward PABs. Incisor tip injury delays PAB differentiation by stimulating LaCL mesenchymal proliferation and altering ECM remodeling within the PAB niche.\n                  <\/jats:p>","DOI":"10.1007\/s00223-026-01571-1","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T01:40:54Z","timestamp":1786671654000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatial Regulation of Preameloblast Development in Response to Distal Mouse Incisor Injury"],"prefix":"10.1007","volume":"117","author":[{"given":"Alexia","family":"Campbell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake","family":"Ngu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cierra Rose","family":"Ahlstrand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terumi","family":"Kohwi-Shigematsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2373-1696","authenticated-orcid":false,"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"issue":"4","key":"1571_CR1","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1016\/j.stem.2020.09.011","volume":"27","author":"E Fuchs","year":"2020","unstructured":"Fuchs E, Blau HM (2020) Tissue stem cells: architects of their niches. Cell Stem Cell 27(4):532\u2013556","journal-title":"Cell Stem Cell"},{"issue":"1","key":"1571_CR2","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1002\/jcp.30562","volume":"237","author":"G Mannino","year":"2022","unstructured":"Mannino G et al (2022) Adult stem cell niches for tissue homeostasis. J Cell Physiol 237(1):239\u2013257","journal-title":"J Cell Physiol"},{"issue":"3","key":"1571_CR3","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1016\/j.cell.2025.12.025","volume":"189","author":"H Clevers","year":"2026","unstructured":"Clevers H (2026) Shifting paradigms in tissue stem cell biology: Insights from the intestine. Cell 189(3):706\u2013724","journal-title":"Cell"},{"issue":"6","key":"1571_CR4","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1016\/S0955-0674(96)80086-X","volume":"8","author":"I Thesleff","year":"1996","unstructured":"Thesleff I, Nieminen P (1996) Tooth morphogenesis and cell differentiation. Curr Opin Cell Biol 8(6):844\u2013850","journal-title":"Curr Opin Cell Biol"},{"issue":"8","key":"1571_CR5","doi-asserted-by":"publisher","first-page":"5008","DOI":"10.1074\/jbc.M509295200","volume":"281","author":"S Fukumoto","year":"2006","unstructured":"Fukumoto S et al (2006) Laminin alpha5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. J Biol Chem 281(8):5008\u20135016","journal-title":"J Biol Chem"},{"issue":"3","key":"1571_CR6","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1111\/j.1601-6343.2005.00331.x","volume":"8","author":"S Shi","year":"2005","unstructured":"Shi S et al (2005) The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res 8(3):191\u2013199","journal-title":"Orthod Craniofac Res"},{"issue":"1","key":"1571_CR7","doi-asserted-by":"publisher","first-page":"e79","DOI":"10.1371\/journal.pone.0000079","volume":"1","author":"W Sonoyama","year":"2006","unstructured":"Sonoyama W et al (2006) Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS ONE 1(1):e79","journal-title":"PLoS ONE"},{"issue":"16","key":"1571_CR8","doi-asserted-by":"publisher","first-page":"6503","DOI":"10.1073\/pnas.1015449108","volume":"108","author":"J Feng","year":"2011","unstructured":"Feng J et al (2011) Dual origin of mesenchymal stem cells contributing to organ growth and repair. Proc Natl Acad Sci U S A 108(16):6503\u20136508","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"13","key":"1571_CR9","doi-asserted-by":"publisher","first-page":"2273","DOI":"10.1242\/dev.134189","volume":"143","author":"PT Sharpe","year":"2016","unstructured":"Sharpe PT (2016) Dental mesenchymal stem cells. Development 143(13):2273\u20132280","journal-title":"Development"},{"issue":"1","key":"1571_CR10","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1038\/s41467-017-02785-6","volume":"9","author":"Z An","year":"2018","unstructured":"An Z et al (2018) A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors. Nat Commun 9(1):378","journal-title":"Nat Commun"},{"issue":"6","key":"1571_CR11","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1007\/s13770-025-00725-w","volume":"22","author":"EM Sedek","year":"2025","unstructured":"Sedek EM, Holiel AA (2025) Next-Generation Strategies for Enamel Repair and Regeneration: Advances in Biomaterials and Translational Challenges. Tissue Eng Regen Med 22(6):771\u2013789","journal-title":"Tissue Eng Regen Med"},{"key":"1571_CR12","doi-asserted-by":"crossref","unstructured":"Yu T, Klein OD (2020) Molecular and cellular mechanisms of tooth development, homeostasis and repair. Development, 147(2)","DOI":"10.1242\/dev.184754"},{"issue":"1","key":"1571_CR13","doi-asserted-by":"publisher","first-page":"4816","DOI":"10.1038\/s41467-020-18512-7","volume":"11","author":"J Krivanek","year":"2020","unstructured":"Krivanek J et al (2020) Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth. Nat Commun 11(1):4816","journal-title":"Nat Commun"},{"issue":"Suppl 1","key":"1571_CR14","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/j.1600-0722.2011.00881.x","volume":"119","author":"RS Lacruz","year":"2011","unstructured":"Lacruz RS et al (2011) Gene-expression analysis of early- and late-maturation-stage rat enamel organ. Eur J Oral Sci 119(Suppl 1):149\u2013157","journal-title":"Eur J Oral Sci"},{"issue":"4","key":"1571_CR15","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1002\/ar.1091790403","volume":"179","author":"H Warshawsky","year":"1974","unstructured":"Warshawsky H, Smith CE (1974) Morphological classification of rat incisor ameloblasts. Anat Rec 179(4):423\u2013446","journal-title":"Anat Rec"},{"issue":"Pt 1","key":"1571_CR16","first-page":"45","volume":"124","author":"K Josephsen","year":"1977","unstructured":"Josephsen K, Fejerskov O (1977) Ameloblast modulation in the maturation zone of the rat incisor enamel organ. A light and electron microscopic study. J Anat 124(Pt 1):45\u201370","journal-title":"J Anat"},{"issue":"Pt 3","key":"1571_CR17","first-page":"381","volume":"133","author":"EJ Reith","year":"1981","unstructured":"Reith EJ, Boyde A (1981) The arrangement of ameloblasts on the surface of maturing enamel of the rat incisor tooth. J Anat 133(Pt 3):381\u2013388","journal-title":"J Anat"},{"issue":"1","key":"1571_CR18","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1089\/ten.tec.2012.0232","volume":"19","author":"MG Chavez","year":"2013","unstructured":"Chavez MG et al (2013) Characterization of dental epithelial stem cells from the mouse incisor with two-dimensional and three-dimensional platforms. Tissue Eng Part C Methods 19(1):15\u201324","journal-title":"Tissue Eng Part C Methods"},{"issue":"7512","key":"1571_CR19","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nature13613","volume":"512","author":"E Harjunmaa","year":"2014","unstructured":"Harjunmaa E et al (2014) Replaying evolutionary transitions from the dental fossil record. Nature 512(7512):44\u201348","journal-title":"Nature"},{"issue":"5","key":"1571_CR20","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/j.celrep.2015.03.064","volume":"11","author":"V Tapaltsyan","year":"2015","unstructured":"Tapaltsyan V et al (2015) Continuously growing rodent molars result from a predictable quantitative evolutionary change over 50 million years. Cell Rep 11(5):673\u2013680","journal-title":"Cell Rep"},{"issue":"6","key":"1571_CR21","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1242\/dev.00332","volume":"130","author":"M Tummers","year":"2003","unstructured":"Tummers M, Thesleff I (2003) Root or crown: a developmental choice orchestrated by the differential regulation of the epithelial stem cell niche in the tooth of two rodent species. Development 130(6):1049\u20131057","journal-title":"Development"},{"issue":"1","key":"1571_CR22","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1083\/jcb.147.1.105","volume":"147","author":"H Harada","year":"1999","unstructured":"Harada H et al (1999) Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling. J Cell Biol 147(1):105\u2013120","journal-title":"J Cell Biol"},{"issue":"4","key":"1571_CR23","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.jdsr.2016.04.001","volume":"52","author":"M Tamura","year":"2016","unstructured":"Tamura M, Nemoto E (2016) Role of the Wnt signaling molecules in the tooth. Jpn Dent Sci Rev 52(4):75\u201383","journal-title":"Jpn Dent Sci Rev"},{"issue":"2","key":"1571_CR24","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.devcel.2015.02.021","volume":"33","author":"J Li","year":"2015","unstructured":"Li J et al (2015) BMP-SHH signaling network controls epithelial stem cell fate via regulation of its niche in the developing tooth. Dev Cell 33(2):125\u2013135","journal-title":"Dev Cell"},{"issue":"9","key":"1571_CR25","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1038\/s41556-019-0378-2","volume":"21","author":"A Sharir","year":"2019","unstructured":"Sharir A et al (2019) A large pool of actively cycling progenitors orchestrates self-renewal and injury repair of an ectodermal appendage. Nat Cell Biol 21(9):1102\u20131112","journal-title":"Nat Cell Biol"},{"key":"1571_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0065-3233(08)60374-5","volume":"39","author":"GR Martin","year":"1988","unstructured":"Martin GR, Timpl R, Kuhn K (1988) Basement membrane proteins: molecular structure and function. Adv Protein Chem 39:1\u201350","journal-title":"Adv Protein Chem"},{"issue":"5","key":"1571_CR27","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1016\/S0955-0674(96)80102-5","volume":"8","author":"R Timpl","year":"1996","unstructured":"Timpl R (1996) Macromolecular organization of basement membranes. Curr Opin Cell Biol 8(5):618\u2013624","journal-title":"Curr Opin Cell Biol"},{"issue":"4","key":"1571_CR28","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","volume":"45","author":"AW Bauer","year":"1966","unstructured":"Bauer AW et al (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45(4):493\u2013496","journal-title":"Am J Clin Pathol"},{"issue":"3","key":"1571_CR29","first-page":"241","volume":"6","author":"I Thesleff","year":"1978","unstructured":"Thesleff I (1978) Role of the basement membrane in odontoblast differentiation. J Biol Buccale 6(3):241\u2013249","journal-title":"J Biol Buccale"},{"issue":"22","key":"1571_CR30","doi-asserted-by":"publisher","first-page":"3753","DOI":"10.1242\/dev.056358","volume":"137","author":"K Seidel","year":"2010","unstructured":"Seidel K et al (2010) Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor. Development 137(22):3753\u20133761","journal-title":"Development"},{"issue":"7","key":"1571_CR31","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1038\/ncb2766","volume":"15","author":"B Biehs","year":"2013","unstructured":"Biehs B et al (2013) BMI1 represses Ink4a\/Arf and Hox genes to regulate stem cells in the rodent incisor. Nat Cell Biol 15(7):846\u2013852","journal-title":"Nat Cell Biol"},{"issue":"1","key":"1571_CR32","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1186\/s12915-019-0722-9","volume":"17","author":"Y Zhang","year":"2019","unstructured":"Zhang Y et al (2019) SATB1 establishes ameloblast cell polarity and regulates directional amelogenin secretion for enamel formation. BMC Biol 17(1):104","journal-title":"BMC Biol"},{"issue":"2","key":"1571_CR33","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1038\/ni.3646","volume":"18","author":"Y Kitagawa","year":"2017","unstructured":"Kitagawa Y et al (2017) Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment. Nat Immunol 18(2):173\u2013183","journal-title":"Nat Immunol"},{"issue":"1","key":"1571_CR34","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.stem.2017.03.023","volume":"21","author":"JK Hu","year":"2017","unstructured":"Hu JK et al (2017) An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice. Cell Stem Cell 21(1):91\u2013106e6","journal-title":"Cell Stem Cell"},{"key":"1571_CR35","doi-asserted-by":"publisher","first-page":"1116091","DOI":"10.3389\/fphys.2023.1116091","volume":"14","author":"B Chan","year":"2023","unstructured":"Chan B et al (2023) Sodium\/(calcium\u2009+\u2009potassium) exchanger NCKX4 optimizes KLK4 activity in the enamel matrix microenvironment to regulate ECM modeling. Front Physiol 14:1116091","journal-title":"Front Physiol"},{"key":"1571_CR36","unstructured":"Huang Y et al (2021) A N-terminus domain determines amelogenin\u2019s stability to guide the development of mouse enamel matrix. J Bone Min Res"},{"issue":"4","key":"1571_CR37","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1016\/S0006-291X(03)01467-0","volume":"308","author":"A Nakata","year":"2003","unstructured":"Nakata A et al (2003) Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. Biochem Biophys Res Commun 308(4):834\u2013839","journal-title":"Biochem Biophys Res Commun"},{"key":"1571_CR38","doi-asserted-by":"publisher","first-page":"277","DOI":"10.3389\/fphys.2014.00277","volume":"5","author":"J Sarkar","year":"2014","unstructured":"Sarkar J et al (2014) Comparison of two mouse ameloblast-like cell lines for enamel-specific gene expression. Front Physiol 5:277","journal-title":"Front Physiol"},{"issue":"2","key":"1571_CR39","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.ajhg.2021.01.007","volume":"108","author":"J den Hoed","year":"2021","unstructured":"den Hoed J et al (2021) Mutation-specific pathophysiological mechanisms define different neurodevelopmental disorders associated with SATB1 dysfunction. Am J Hum Genet 108(2):346\u2013356","journal-title":"Am J Hum Genet"},{"issue":"Pt 4","key":"1571_CR40","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1242\/jcs.92.4.531","volume":"92","author":"R Verheijen","year":"1989","unstructured":"Verheijen R et al (1989) Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II. Localization in mitotic cells and association with chromosomes. J Cell Sci 92(Pt 4):531\u2013540","journal-title":"J Cell Sci"},{"key":"1571_CR41","doi-asserted-by":"publisher","first-page":"e01641","DOI":"10.7554\/eLife.01641","volume":"3","author":"DG Booth","year":"2014","unstructured":"Booth DG et al (2014) Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:e01641","journal-title":"Elife"},{"key":"1571_CR42","doi-asserted-by":"crossref","unstructured":"Andres-Sanchez N, Fisher D, Krasinska L (2022) Physiological functions and roles in cancer of the proliferation marker Ki-67. J Cell Sci, 135(11)","DOI":"10.1242\/jcs.258932"},{"issue":"2","key":"1571_CR43","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1002\/ar.1092240206","volume":"224","author":"A Linde","year":"1989","unstructured":"Linde A (1989) Dentin matrix proteins: composition and possible functions in calcification. Anat Rec 224(2):154\u2013166","journal-title":"Anat Rec"},{"issue":"9","key":"1571_CR44","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1111\/j.1365-2591.2007.01263.x","volume":"40","author":"G Orsini","year":"2007","unstructured":"Orsini G et al (2007) Immunohistochemical identification of type I and type III collagen and chondroitin sulphate in human pre-dentine: a correlative FEI-SEM\/TEM study. Int Endod J 40(9):669\u2013678","journal-title":"Int Endod J"},{"issue":"17","key":"1571_CR45","doi-asserted-by":"publisher","first-page":"1823","DOI":"10.1091\/mbc.E19-12-0709","volume":"31","author":"VW Tang","year":"2020","unstructured":"Tang VW (2020) Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology. Mol Biol Cell 31(17):1823\u20131834","journal-title":"Mol Biol Cell"},{"key":"1571_CR46","doi-asserted-by":"crossref","unstructured":"Sun B (2021) The mechanics of fibrillar collagen extracellular matrix. Cell Rep Phys Sci, 2(8)","DOI":"10.1016\/j.xcrp.2021.100515"},{"key":"1571_CR47","doi-asserted-by":"publisher","first-page":"p7602951","DOI":"10.1155\/2017\/7602951","volume":"2017","author":"E Rangel-Huerta","year":"2017","unstructured":"Rangel-Huerta E, Maldonado E (2017) Transit-Amplifying Cells in the Fast Lane from Stem Cells towards Differentiation. Stem Cells Int 2017:p7602951","journal-title":"Stem Cells Int"},{"issue":"6","key":"1571_CR48","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1016\/j.stem.2010.11.016","volume":"7","author":"N Barker","year":"2010","unstructured":"Barker N, Bartfeld S, Clevers H (2010) Tissue-resident adult stem cell populations of rapidly self-renewing organs. Cell Stem Cell 7(6):656\u2013670","journal-title":"Cell Stem Cell"},{"key":"1571_CR49","doi-asserted-by":"publisher","first-page":"1189225","DOI":"10.3389\/fbioe.2023.1189225","volume":"11","author":"R Cancedda","year":"2023","unstructured":"Cancedda R, Mastrogiacomo M (2023) Transit Amplifying Cells (TACs): a still not fully understood cell population. Front Bioeng Biotechnol 11:1189225","journal-title":"Front Bioeng Biotechnol"},{"issue":"10","key":"1571_CR50","doi-asserted-by":"publisher","first-page":"e12912","DOI":"10.1111\/cpr.12912","volume":"53","author":"SS Jin","year":"2020","unstructured":"Jin SS et al (2020) Mechanical force modulates periodontal ligament stem cell characteristics during bone remodelling via TRPV4. Cell Prolif 53(10):e12912","journal-title":"Cell Prolif"},{"key":"1571_CR51","doi-asserted-by":"crossref","unstructured":"Gauthier R et al (2021) Tissue engineering for periodontal ligament regeneration: biomechanical specifications. J Biomech Eng, 143(3)","DOI":"10.1115\/1.4048810"},{"issue":"2","key":"1571_CR52","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.stem.2013.12.013","volume":"14","author":"H Zhao","year":"2014","unstructured":"Zhao H et al (2014) Secretion of shh by a neurovascular bundle niche supports mesenchymal stem cell homeostasis in the adult mouse incisor. Cell Stem Cell 14(2):160\u2013173","journal-title":"Cell Stem Cell"},{"key":"1571_CR53","doi-asserted-by":"publisher","first-page":"724098","DOI":"10.3389\/fphys.2021.724098","volume":"12","author":"T Liang","year":"2021","unstructured":"Liang T et al (2021) Enamel defects associated with dentin sialophosphoprotein mutation in mice. Front Physiol 12:724098","journal-title":"Front Physiol"},{"issue":"4","key":"1571_CR54","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1038\/ijos.2012.69","volume":"4","author":"A Rangiani","year":"2012","unstructured":"Rangiani A et al (2012) Dentin matrix protein 1 and phosphate homeostasis are critical for postnatal pulp, dentin and enamel formation. Int J Oral Sci 4(4):189\u2013195","journal-title":"Int J Oral Sci"},{"issue":"1","key":"1571_CR55","doi-asserted-by":"publisher","first-page":"20653","DOI":"10.1038\/s41598-021-00219-4","volume":"11","author":"T Liang","year":"2021","unstructured":"Liang T et al (2021) Mouse Dspp frameshift model of human dentinogenesis imperfecta. Sci Rep 11(1):20653","journal-title":"Sci Rep"},{"issue":"5","key":"1571_CR56","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/j.matbio.2010.03.001","volume":"29","author":"P He","year":"2010","unstructured":"He P et al (2010) Ameloblast differentiation in the human developing tooth: effects of extracellular matrices. Matrix Biol 29(5):411\u2013419","journal-title":"Matrix Biol"},{"issue":"11","key":"1571_CR57","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1007\/BF02388111","volume":"25","author":"M Goldberg","year":"1993","unstructured":"Goldberg M, Takagi M (1993) Dentine proteoglycans: composition, ultrastructure and functions. Histochem J 25(11):781\u2013806","journal-title":"Histochem J"},{"issue":"11","key":"1571_CR58","doi-asserted-by":"publisher","first-page":"2897","DOI":"10.1111\/j.1742-4658.2007.05823.x","volume":"274","author":"J Candiello","year":"2007","unstructured":"Candiello J et al (2007) Biomechanical properties of native basement membranes. FEBS J 274(11):2897\u20132908","journal-title":"FEBS J"},{"issue":"6","key":"1571_CR59","doi-asserted-by":"publisher","first-page":"2204","DOI":"10.1529\/biophysj.107.111013","volume":"94","author":"L Yang","year":"2008","unstructured":"Yang L et al (2008) Mechanical properties of native and cross-linked type I collagen fibrils. Biophys J 94(6):2204\u20132211","journal-title":"Biophys J"},{"issue":"3","key":"1571_CR60","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.bpj.2014.12.023","volume":"108","author":"JC Kohn","year":"2015","unstructured":"Kohn JC et al (2015) Cooperative effects of matrix stiffness and fluid shear stress on endothelial cell behavior. Biophys J 108(3):471\u2013478","journal-title":"Biophys J"},{"issue":"3","key":"1571_CR61","doi-asserted-by":"publisher","first-page":"e1010902","DOI":"10.1371\/journal.pcbi.1010902","volume":"19","author":"M Pensalfini","year":"2023","unstructured":"Pensalfini M, Tepole AB (2023) Mechano-biological and bio-mechanical pathways in cutaneous wound healing. PLoS Comput Biol 19(3):e1010902","journal-title":"PLoS Comput Biol"},{"issue":"12","key":"1571_CR62","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1177\/00220345251336164","volume":"104","author":"Y Zheng","year":"2025","unstructured":"Zheng Y et al (2025) Epithelial YAP1 Regulates Ameloblast Differentiation through SHH\/FGF Signaling. J Dent Res 104(12):1395\u20131404","journal-title":"J Dent Res"},{"issue":"6","key":"1571_CR63","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1096\/fasebj.4.6.2180767","volume":"4","author":"PD Yurchenco","year":"1990","unstructured":"Yurchenco PD, Schittny JC (1990) Molecular architecture of basement membranes. FASEB J 4(6):1577\u20131590","journal-title":"FASEB J"},{"issue":"1\u20132","key":"1571_CR64","first-page":"93","volume":"27","author":"M Paulsson","year":"1992","unstructured":"Paulsson M (1992) Basement membrane proteins: structure, assembly, and cellular interactions. Crit Rev Biochem Mol Biol 27(1\u20132):93\u2013127","journal-title":"Crit Rev Biochem Mol Biol"},{"issue":"5","key":"1571_CR65","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/S0955-0674(02)00371-X","volume":"14","author":"MA Arnaout","year":"2002","unstructured":"Arnaout MA, Goodman SL, Xiong JP (2002) Coming to grips with integrin binding to ligands. Curr Opin Cell Biol 14(5):641\u2013651","journal-title":"Curr Opin Cell Biol"},{"issue":"7","key":"1571_CR66","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1046\/j.1538-7836.2003.00277.x","volume":"1","author":"JP Xiong","year":"2003","unstructured":"Xiong JP et al (2003) Integrins, cations and ligands: making the connection. J Thromb Haemost 1(7):1642\u20131654","journal-title":"J Thromb Haemost"}],"container-title":["Calcified Tissue International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00223-026-01571-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00223-026-01571-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00223-026-01571-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T03:59:23Z","timestamp":1786679963000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00223-026-01571-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":66,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["1571"],"URL":"https:\/\/doi.org\/10.1007\/s00223-026-01571-1","relation":{},"ISSN":["1432-0827"],"issn-type":[{"value":"1432-0827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]},"assertion":[{"value":"26 February 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All authors have no financial or non-financial interests to declare.","order":1,"name":"Ethics","label":"<b>Conflict of Interests<\/b>","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal studies relevant to this publication were all reviewed and approved by the University of California, San Francisco Institutional Animal and Care Use Committee (IACUC).","order":2,"name":"Ethics","label":"Ethical Approval","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"129"}}