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Remarkably, no environmental or locally transmitted clinical case of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    has been reported in Denmark for more than 100\u00a0years. We, however, have recovered a near-complete genome out of 115 metagenomic sewage samples taken over the past 8\u00a0years, despite the extremely low relative abundance of one\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    read out of 500,000 sequenced reads. Due to the very low relative abundance, routine screening of the individual samples did not reveal\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . The recovered genome lacks the gene responsible for cholerae toxin production, but although this strain may not pose an immediate public health risk, our finding illustrates the importance, challenges, and effectiveness of wastewater-based pathogen surveillance.\n                  <\/jats:p>","DOI":"10.1007\/s00248-024-02419-7","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T16:02:29Z","timestamp":1722441749000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Discovery of Vibrio cholerae in Urban Sewage in Copenhagen, Denmark"],"prefix":"10.1007","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5074-7183","authenticated-orcid":false,"given":"Christian","family":"Brinch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2538-8086","authenticated-orcid":false,"given":"Saria","family":"Otani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8813-4019","authenticated-orcid":false,"given":"Patrick","family":"Munk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaike","family":"van den Beld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eelco","family":"Franz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank M.","family":"Aarestrup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"issue":"1","key":"2419_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/MBIO.02703-20\/ASSET\/1FCC5B74-F52F-4B47-82D7-4EC4764697A2\/ASSETS\/GRAPHIC\/MBIO.02703-20-F0003.JPEG","volume":"12","author":"A Crits-Christoph","year":"2021","unstructured":"Crits-Christoph A et al (2021) Genome sequencing of sewage detects regionally prevalent SARS-CoV-2 variants. mBio 12(1):1\u20139. https:\/\/doi.org\/10.1128\/MBIO.02703-20\/ASSET\/1FCC5B74-F52F-4B47-82D7-4EC4764697A2\/ASSETS\/GRAPHIC\/MBIO.02703-20-F0003.JPEG","journal-title":"mBio"},{"issue":"1","key":"2419_CR2","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1038\/S41467-019-08853-3","volume":"10","author":"RS Hendriksen","year":"2019","unstructured":"Hendriksen RS et al (2019) Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage. 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