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Nodavirus (NNV) is one of the most prevalent viruses worldwide causing disease in more than 170 species. In this study, we aimed to develop a non-lethal method to diagnose NNV in the gonad of asymptomatic broodstock specimens of greater amberjack (\n                    <jats:italic>Seriola dumerili<\/jats:italic>\n                    ) using a RTG cell line expressing the luciferase reporter gene under the control of the Mx promoter (RTG-pmx-luciferase system) and real-time PCR. We also characterize the immune response and reproductive health of both sexes by means of gene expression and functional parameters analysis. Our data showed that NNV is present in both male and female blood and gonads in similar proportions demonstrating that both sexes might develop persistent infection and transfer the virus to their progeny by vertical transmission. Regarding immune response, the levels observed in the testis were higher in infected males. Specifically, we observed an up-regulation of genes related to the interferon pathway, antimicrobial peptides and leucocyte-markers molecules in infected testis, which might lead to a functional alteration. Otherwise, there were no alterations of immune parameters in the infected ovaries suggesting a latent infection. Serum estradiol levels were high in infected females constituting a potential mechanism to fight against the virus. In conclusion, we optimized a non-lethal method for NNV diagnosis involving gonadal biopsies. Moreover, our study revealed sex-biased host-NNV interactions.\n                  <\/jats:p>","DOI":"10.1007\/s00248-026-02733-2","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T09:02:22Z","timestamp":1773219742000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diagnosis of Betanodavirus Infection in the Gonad of Greater Amberjack Broodstocks Shows a Sex-Biased Infection and Immune Responses"],"prefix":"10.1007","volume":"89","author":[{"given":"L.","family":"Cervera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"\u00c1lvarez-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Barreto-Bailet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"B\u00e9jar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Cuesta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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